Standard name
Human Ortholog
Description NADPH-dependent quinone reductase; GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.94 0.94 0.92 0.85 0.75 0.76 0.65 0.74 0.65 0.56 0.99 0.99 0.98 0.96 0.97 0.97 0.96 0.94 0.87 0.94 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.07 0.17 0.07 0.25 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.08 0.08 0.13 0.19 0.2 0.23 0.16 0.17 0.29 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 1 0 1 0 1 0 0 0 0 0 0 1 0 0 0 1 1
Bud 0 0 0 1 0 0 4 4 4 2 4 2 0 0 0 0 0 0 1 0 1 0 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 1 2 5 9 8 4 5 10 5 10 0 5 3 15 6 8 0 0 0 0 0 0
Cytoplasm 316 65 76 201 313 292 390 327 209 248 170 140 304 389 456 309 116 318 309 73 96 242 365 390
Endoplasmic Reticulum 2 0 2 1 5 1 8 4 2 7 3 2 1 1 4 13 3 15 0 0 2 3 1 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3 0 0 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 5 1 3 0 0 0 1 0 0
Mitochondria 2 1 1 1 5 14 39 29 53 24 64 34 0 0 0 0 2 1 0 0 0 0 1 2
Nucleus 1 0 2 2 2 2 10 16 6 13 10 6 2 1 5 0 0 1 0 1 2 1 0 2
Nuclear Periphery 1 0 0 0 0 2 1 1 1 1 2 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3
Vac/Vac Membrane 1 0 1 17 26 43 97 86 75 54 44 73 0 6 2 9 0 4 1 1 2 1 0 3
Unique Cell Count 321 66 81 213 339 342 518 432 320 337 260 249 307 392 463 322 119 328 324 78 111 258 384 419
Labelled Cell Count 325 66 83 225 356 364 558 472 355 360 302 269 308 404 470 353 128 350 324 78 111 258 384 419


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 9.9 9.6 9.5 10.0 8.9 8.7 9.1 8.3 8.7 8.1 8.9 12.5 13.2 14.8 34.9 34.8 33.7 9.8 9.7 9.7
Std Deviation (1e-4) 1.5 2.3 1.4 2.0 1.9 1.6 1.7 2.0 2.0 1.9 2.0 2.3 2.8 3.1 3.4 8.2 8.4 9.3 2.5 2.4 2.1
Intensity Change (Log2) – – – -0.02 0.06 -0.11 -0.15 -0.08 -0.21 -0.14 -0.25 -0.11 0.38 0.46 0.63 1.86 1.86 1.81 0.03 0.01 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -0.5 -2.0 -3.7 -3.6 -5.1 -3.9 -5.0 -6.2 2.9 3.4 2.6 0.8 1.3 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.1 0 3.6 0 4.7 3.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.1 3.0 4.0 4.1 4.6 3.5 3.6 5.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4095 10.7765 8.8535 8.3409 7.0611 9.6133 5.0225 7.7821 6.1407 5.0498 4.1391 6.6258 7.3213 10.2169 8.6037 7.4089 6.9006 8.9917
Actin 0.009 0.0011 0.0049 0.0338 0.0084 0.0011 0.0273 0.004 0.0371 0.0372 0.0266 0.0083 0.0189 0.0001 0.0186 0.0259 0.0723 0.0085
Bud 0.0137 0.0056 0.0163 0.0016 0.002 0.0094 0.0005 0.0035 0.0007 0.0006 0.0006 0.0004 0.0007 0.0001 0.0009 0.0007 0.002 0.0003
Bud Neck 0.0014 0.0002 0.0021 0.0009 0.0024 0.0013 0.0012 0.0003 0.0033 0.0233 0.0015 0.0013 0.0017 0.0001 0.0009 0.0016 0.0021 0.0009
Bud Periphery 0.0045 0.001 0.0051 0.0023 0.0041 0.0042 0.0005 0.0007 0.0006 0.0004 0.0006 0.0004 0.0005 0 0.0008 0.0003 0.0023 0.0002
Bud Site 0.006 0.0029 0.0205 0.0066 0.0014 0.0007 0.0014 0.0062 0.0031 0.0017 0.002 0.0002 0.0011 0.0002 0.0022 0.0017 0.0027 0.0004
Cell Periphery 0.0005 0.0001 0.0006 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0003 0
Cytoplasm 0.7118 0.9519 0.8128 0.6383 0.7987 0.9185 0.7504 0.9538 0.8828 0.8357 0.545 0.9279 0.8222 0.9884 0.9122 0.8501 0.6368 0.921
Cytoplasmic Foci 0.0311 0.004 0.0257 0.0293 0.0054 0.0096 0.0363 0.0045 0.0097 0.0142 0.0805 0.0088 0.0486 0.0009 0.0089 0.0509 0.0251 0.0135
Eisosomes 0.0001 0 0.0001 0.0003 0.0001 0 0.0001 0 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0003 0
Endoplasmic Reticulum 0.0085 0.0015 0.0085 0.0038 0.0161 0.0021 0.0062 0.0011 0.0038 0.0023 0.0064 0.0035 0.0056 0.0007 0.0023 0.0026 0.0049 0.0017
Endosome 0.0722 0.0016 0.0174 0.0925 0.0328 0.0036 0.0369 0.0051 0.0102 0.0283 0.0532 0.0063 0.0218 0.0003 0.0143 0.0073 0.0478 0.0121
Golgi 0.005 0.0001 0.0048 0.0526 0.0051 0.0002 0.0132 0.0018 0.0032 0.0116 0.0188 0.0021 0.0052 0 0.0044 0.0037 0.0279 0.0041
Lipid Particles 0.0163 0.0003 0.0012 0.0576 0.0011 0.0001 0.0236 0.001 0.0011 0.0018 0.0569 0.0016 0.0197 0 0.0087 0.0072 0.0266 0.0024
Mitochondria 0.0044 0.0004 0.0098 0.0251 0.0093 0.0005 0.0038 0.0005 0.0014 0.0029 0.0171 0.0041 0.0039 0 0.0021 0.0007 0.0591 0.0008
None 0.0196 0.0056 0.0188 0.0055 0.0033 0.004 0.0356 0.0036 0.0144 0.0216 0.0077 0.0073 0.0136 0.0039 0.0033 0.0055 0.0024 0.0014
Nuclear Periphery 0.0135 0.0023 0.0062 0.0077 0.042 0.0036 0.0111 0.0013 0.0098 0.002 0.0481 0.0062 0.0024 0.0008 0.0034 0.0027 0.0147 0.0029
Nucleolus 0.0024 0.0001 0.0014 0.0006 0.0005 0.0001 0.0011 0.0001 0.0001 0.0001 0.0011 0.0001 0.0007 0 0.0002 0.0001 0.0004 0
Nucleus 0.0408 0.0181 0.0336 0.0138 0.046 0.0313 0.014 0.0092 0.0127 0.0117 0.0183 0.0153 0.0089 0.0041 0.0078 0.0153 0.0074 0.023
Peroxisomes 0.0033 0.0001 0.0032 0.0136 0.0002 0.0007 0.0209 0.0011 0.0016 0.0021 0.0357 0.0009 0.017 0 0.0013 0.0154 0.0565 0.004
Punctate Nuclear 0.0168 0.0009 0.0041 0.0058 0.002 0.0075 0.0119 0.0012 0.0022 0.0009 0.038 0.0027 0.0044 0.0002 0.0028 0.0054 0.003 0.0013
Vacuole 0.016 0.0019 0.0022 0.0053 0.0107 0.0012 0.0025 0.0008 0.0013 0.0009 0.0245 0.0017 0.0023 0.0002 0.0034 0.0024 0.0026 0.0012
Vacuole Periphery 0.003 0.0002 0.0007 0.0024 0.0082 0.0003 0.0012 0.0001 0.0007 0.0005 0.017 0.0007 0.0005 0 0.0014 0.0004 0.0028 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.3885 19.6294 28.4034 32.2546 18.5054 36.2111 55.3242 56.9311 54.7153 34.7889
Translational Efficiency 2.7499 3.506 2.2406 2.7216 2.0804 3.6523 2.3932 2.0737 2.2796 2.042

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
294 155 2770 3 1515 53 2190 1691 1809 208 4960 1694

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1178.92 1231.92 1464.98 3338.69 1057.15 295.61 1010.73 913.57 1076.94 993.34 1264.41 917.86
Standard Deviation 258.18 264.71 253.80 741.53 230.06 12.97 142.46 168.97 239.12 467.68 309.54 199.67
Intensity Change Log 2 — 0.063443 0.313415 1.501816 — -1.838413 -0.064782 -0.210593 — -0.549765 0.146877 0.927264

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000251 0.000468 0.000132 0.000490 0.000137 0.000031 0.000074 0.000317 0.000156 0.000357 0.000107 0.000317
Bud Neck 0.001062 0.001771 0.001241 0.000126 0.001169 0.000008 0.002158 0.012582 0.001151 0.001322 0.001646 0.012560
Bud Site 0.007713 0.018652 0.002187 0.006179 0.005543 0.000022 0.004365 0.015282 0.005895 0.013905 0.003149 0.015266
Cell Periphery 0.000161 0.000056 0.000080 0.001049 0.000065 0.000077 0.000049 0.000144 0.000081 0.000061 0.000066 0.000145
Cytoplasm 0.789414* 0.758563* 0.769581* 0.962757* 0.850391* 0.000037 0.814287* 0.591268* 0.840481* 0.565285* 0.789320* 0.591926*
Cytoplasmic Foci 0.098338 0.050414 0.006143 0.000837 0.043853 0.000014 0.024209 0.018620 0.052708 0.037572 0.014120 0.018589
Eisosomes 0.000026 0.000010 0.000002 0.000002 0.000007 0.000084 0.000010 0.000029 0.000010 0.000029 0.000006 0.000028
Endoplasmic Reticulum 0.001017 0.000157 0.000378 0.000003 0.000514 0.000001 0.000364 0.002254 0.000595 0.000118 0.000372 0.002250
Endosome 0.005727 0.003271 0.000418 0.000004 0.001123 0.000001 0.001925 0.005351 0.001871 0.002438 0.001083 0.005341
Golgi 0.001043 0.010142 0.000169 0.000083 0.000885 0.000005 0.000117 0.000978 0.000911 0.007559 0.000146 0.000977
Lipid Particles 0.001443 0.000311 0.000417 0.000074 0.000406 0.000022 0.000314 0.001350 0.000575 0.000237 0.000371 0.001347
Mitochondria 0.000081 0.003954 0.000285 0.000007 0.000831 0.000010 0.000124 0.001139 0.000709 0.002949 0.000214 0.001136
Mitotic Spindle 0.000802 0.023848 0.003850 0.000098 0.003009 0.000262 0.001764 0.027123 0.002650 0.017838 0.002929 0.027075
None 0.003736 0.013304 0.005412 0.014575 0.004854 0.999259* 0.002842 0.008603 0.004672 0.264533* 0.004277 0.008614
Nuclear Periphery 0.000108 0.000096 0.000332 0.000007 0.000086 0.000000 0.000194 0.001573 0.000089 0.000072 0.000271 0.001571
Nuclear Periphery Foci 0.005865 0.001563 0.001215 0.004273 0.001997 0.000037 0.001023 0.001514 0.002626 0.001174 0.001130 0.001519
Nucleolus 0.000160 0.000089 0.000131 0.000032 0.000150 0.000029 0.000258 0.000604 0.000152 0.000073 0.000187 0.000603
Nucleus 0.034266 0.060254 0.192002* 0.007246 0.061046 0.000011 0.070123 0.224980* 0.056693 0.044904 0.138188* 0.224595*
Peroxisomes 0.000403 0.003997 0.000032 0.000022 0.000236 0.000089 0.000218 0.001423 0.000263 0.003001 0.000114 0.001420
Vacuole 0.048072 0.047959 0.015856 0.002128 0.022374 0.000001 0.075413 0.084420 0.026550 0.035739 0.042152 0.084274
Vacuole Periphery 0.000311 0.001119 0.000139 0.000009 0.001326 0.000000 0.000169 0.000446 0.001161 0.000834 0.000152 0.000446

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN 6.65 1.83 -5.79 -9.55 -6.44 -0.16 1.29 -5.43 -2.69 -6.15
Bud Neck NaN NaN NaN NaN NaN 8.08 -2.27 -7.43 -8.49 -6.42 1.00 -3.47 -7.46 -7.60 -6.78
Bud Site NaN NaN NaN NaN NaN 8.46 1.95 -4.12 -9.11 -5.55 -0.56 4.18 -4.01 -1.26 -6.40
Cell Periphery NaN NaN NaN NaN NaN -0.63 4.25 -8.54 -3.11 -11.72 3.81 1.53 -6.50 -9.15 -8.04
Cytoplasm NaN NaN NaN NaN NaN 190.17* 8.97 41.33 -73.02* 34.74* 10.57 14.41 40.64 1.47 34.61
Cytoplasmic Foci NaN NaN NaN NaN NaN 19.15 7.25 11.16 -17.75 6.80 5.33 15.88 14.74 1.95 -0.81
Eisosomes NaN NaN NaN NaN NaN -2.46 -5.08 -19.75 1.50 -17.20 -2.23 5.68 -17.09 -0.99 -21.21
Endoplasmic Reticulum NaN NaN NaN NaN NaN 6.83 1.84 -15.07 -20.05 -17.38 6.54 2.38 -14.72 -19.22 -17.11
Endosome NaN NaN NaN NaN NaN 9.13 -3.09 -7.29 -10.44 -5.57 1.60 4.09 -5.40 -5.53 -7.59
Golgi NaN NaN NaN NaN NaN 2.49 2.17 0.59 -2.38 -1.94 -0.73 2.49 0.72 0.86 -1.78
Lipid Particles NaN NaN NaN NaN NaN 7.19 1.10 -11.62 -18.53 -14.11 3.95 0.70 -9.38 -14.06 -11.81
Mitochondria NaN NaN NaN NaN NaN 1.56 1.34 -0.42 -7.40 -6.57 -0.36 1.04 -0.75 -0.05 -5.66
Mitotic Spindle NaN NaN NaN NaN NaN 3.39 1.24 -8.01 -9.42 -8.71 -1.11 -2.06 -8.24 -3.46 -7.76
None NaN NaN NaN NaN NaN -2543.22* 4.70 -2.10 1241.82* -4.70 -8.55 1.31 -2.35 8.48 -3.10
Nuclear Periphery NaN NaN NaN NaN NaN 11.43 -12.01 -29.00 -30.36* -26.95* -2.08 -20.10 -28.97 -26.47 -24.89
Nuclear Periphery Foci NaN NaN NaN NaN NaN 10.53 4.57 1.25 -14.71 -4.61 4.30 6.13 3.81 -2.07 -3.10
Nucleolus NaN NaN NaN NaN NaN 3.90 -1.94 -3.69 -4.70 -2.93 2.30 -1.65 -3.70 -4.25 -3.35
Nucleus NaN NaN NaN NaN NaN 29.34 -4.23 -33.53 -46.65 -31.85* 3.28 -31.55 -34.74 -28.61 -19.98
Peroxisomes NaN NaN NaN NaN NaN 4.56 0.63 -2.65 -3.17 -2.74 -1.69 4.33 -2.57 0.35 -3.07
Vacuole NaN NaN NaN NaN NaN 16.32 -19.32 -22.26 -29.74 -7.59 -5.49 -11.67 -21.56 -2.50 -16.14
Vacuole Periphery NaN NaN NaN NaN NaN 2.26 1.98 1.64 -7.27 -3.76 1.56 2.05 1.63 -0.19 -3.97
Standard name
Human Ortholog
Description NADPH-dependent quinone reductase; GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Zta1

Zta1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zta1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available