Standard name | |
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Human Ortholog | |
Description | Adhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 5 | 1 | 1 | 5 | – | 14 | 3 | 16 | 7 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 7 | 5 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 129 | 123 | 197 | 229 | 232 | 376 | – | 492 | 389 | 483 | 330 | 315 | 208 | 279 | 359 | 136 | 120 | 132 | 38 | 28 | 34 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 2 | 2 | 4 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 8 | 7 | 12 | 2 | 0 | 5 | 0 | 1 | 0 |
Endosome | 23 | 3 | 2 | 0 | 4 | 2 | – | 2 | 1 | 2 | 0 | 0 | 1 | 3 | 1 | 1 | 1 | 2 | 13 | 10 | 15 | 41 | 23 | 45 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 4 | 1 | 0 | 3 | 6 |
Mitochondria | 46 | 4 | 78 | 54 | 17 | 74 | – | 85 | 131 | 143 | 123 | 131 | 0 | 1 | 1 | 4 | 2 | 4 | 22 | 4 | 10 | 0 | 2 | 5 |
Nucleus | 24 | 11 | 18 | 14 | 38 | 31 | – | 31 | 12 | 8 | 7 | 2 | 5 | 1 | 1 | 1 | 2 | 4 | 4 | 0 | 1 | 1 | 6 | 6 |
Nuclear Periphery | 5 | 0 | 0 | 1 | 0 | 2 | – | 9 | 4 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 10 |
Nucleolus | 3 | 1 | 1 | 1 | 0 | 0 | – | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 11 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 3 | 0 | 0 | 0 | – | 6 | 1 | 4 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 3 | 3 | 3 | 2 | 0 |
Vac/Vac Membrane | 210 | 111 | 100 | 99 | 139 | 138 | – | 101 | 77 | 60 | 46 | 33 | 26 | 15 | 7 | 3 | 3 | 7 | 309 | 161 | 293 | 92 | 133 | 201 |
Unique Cell Count | 378 | 219 | 332 | 316 | 367 | 517 | 641 | 491 | 607 | 417 | 416 | 231 | 295 | 367 | 143 | 128 | 145 | 421 | 230 | 380 | 152 | 190 | 294 | |
Labelled Cell Count | 446 | 256 | 406 | 403 | 431 | 628 | 749 | 618 | 722 | 518 | 504 | 243 | 302 | 372 | 155 | 137 | 163 | 421 | 230 | 380 | 152 | 190 | 294 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 5.7 | 3.8 | 3.9 | 5.2 | 4.1 | – | 4.0 | 3.3 | 3.5 | 3.3 | 3.1 | 4.7 | 4.8 | 4.5 | 5.7 | 5.6 | 6.0 | 11.3 | 12.8 | 13.7 |
Std Deviation (1e-4) | 4.2 | 1.4 | 1.0 | 1.3 | 2.4 | 2.3 | – | 1.5 | 1.3 | 1.3 | 1.0 | 1.1 | 1.1 | 1.2 | 1.1 | 1.8 | 1.6 | 2.6 | 3.2 | 4.8 | 4.5 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.44 | 0.09 | – | 0.05 | -0.23 | -0.14 | -0.23 | -0.33 | 0.28 | 0.32 | 0.22 | 0.57 | 0.55 | 0.65 | 1.56 | 1.74 | 1.84 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.5 | 1.1 | 4.1 | – | 5.7 | 6.2 | 6.6 | 5.9 | 4.8 | 8.0 | 10.3 | 12.6 | 7.8 | 7.1 | 6.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -7.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.5 | -5.6 | -5.5 |
Nucleus | -0.6 | 2.4 | 0.4 | – | -0.4 | -2.2 | -3.7 | -2.8 | -4.2 | -1.9 | -3.7 | -4.2 | -2.4 | -1.8 | -1.3 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.3 | 2.2 | -1.1 | – | -5.2 | -4.9 | -7.9 | -6.6 | -7.9 | -5.3 | -8.1 | -10.3 | -6.8 | -6.4 | -6.1 |