To retrieve micrographs, protein localization and abundance data:
Type in the ORF or common name of the protein(s) of interest. Users are allowed to input more than
one protein. Users can also paste in a list of proteins on the search box.
Hit the search button.
For details regarding CYCLoPs, click the
About link
Clicking the micrographs thumbnail opens up a modal which contains images
from 21 screens.
Each screen has 4 fields. Slide through different fields by doing
either of the following:
click the left or right icon
click the dots at the bottom of the image
click and hold on the image then swipe left or right
Toggle channel display by choosing either Overlay, GFP or
RFP on the modal header. By default, the overlay image is displayed.
For a closer look on the cells, enable the magnifying glass by
clicking on the icon. To disable it, just click again on the icon.
To adjust the image brightness, click on the
icon and use the slider to increase or decrease the brightness.
SUB-CELLULAR LOCALIZATION
Displays the LOC-score (quantitative measurement of localization) of the
protein in each screen.
The LOC-score is calculated from the proportion of cells that exhibit
morphology specific to a localization class.
If the column is left blank, it means than insufficient cells were obtained
in the screen to make a definitive measurement of localization changes.
Toggle between LOC-score and Cell count to see percentage vs raw data.
YEAST-GFP LOCALIZATION
Shows the localization assignment determined manually by 2 annotators in
previous study - W.-K. Huh, J.V. Falvo, L.C. Gerke, A.S. Carroll, R.W. Howson, J.S. Weissman & E.K.
O'Shea, Global Analysis of Protein
Localization in Budding Yeast, Nature, 425, 686-691 (2003).
PROTEIN ABUNDANCE
Displays the GFP intensity measures of the protein in each screen as a
proxy of protein abundance.
Click the Plot time-course
intensity button to open up a collapsible element which shows subplots of intensity levels across
time-points for each of the treatment conditions: rapamycin, hydroxy-urea,
rpd3 knockout and alpha-factor. Hide the plots by clicking on the button again.
LOCALIZATION CHANGE
The z-LOC scores quantify changes in sub-cellular compartments with respect
to wildtype LOC-scores.
A negative z-LOC value denotes the exit of a
protein from a particular compartment while a positive z-LOC value
represents movement towards a specific location.