HOME PAGE

  • To retrieve micrographs, protein localization and abundance data:

    1. Type in the ORF or common name of the protein(s) of interest. Users are allowed to input more than one protein. Users can also paste in a list of proteins on the search box.
    2. Hit the search button.

  • For details regarding CYCLoPs, click the About link

  • For the user's guide, click the Help link

  • To download files, click the Supplemental Files link

MICROGRAPHS
  • Clicking the micrographs thumbnail opens up a modal which contains images from 21 screens.
  • Each screen has 4 fields. Slide through different fields by doing either of the following:
    • click the left or right icon
    • click the dots at the bottom of the image
    • click and hold on the image then swipe left or right
  • Toggle channel display by choosing either Overlay, GFP or RFP on the modal header. By default, the overlay image is displayed.
  • For a closer look on the cells, enable the magnifying glass by clicking on the icon. To disable it, just click again on the icon.
  • To adjust the image brightness, click on the icon and use the slider to increase or decrease the brightness.
SUB-CELLULAR LOCALIZATION
  • Displays the LOC-score (quantitative measurement of localization) of the protein in each screen.
  • The LOC-score is calculated from the proportion of cells that exhibit morphology specific to a localization class.
  • If the column is left blank, it means than insufficient cells were obtained in the screen to make a definitive measurement of localization changes.
  • Toggle between LOC-score and Cell count to see percentage vs raw data.
YEAST-GFP LOCALIZATION
PROTEIN ABUNDANCE
  • Displays the GFP intensity measures of the protein in each screen as a proxy of protein abundance.
  • Click the Plot time-course intensity button to open up a collapsible element which shows subplots of intensity levels across time-points for each of the treatment conditions: rapamycin, hydroxy-urea, rpd3 knockout and alpha-factor. Hide the plots by clicking on the button again.
LOCALIZATION CHANGE
  • The z-LOC scores quantify changes in sub-cellular compartments with respect to wildtype LOC-scores.
  • A negative z-LOC value denotes the exit of a protein from a particular compartment while a positive z-LOC value represents movement towards a specific location.