HOME PAGE
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To retrieve micrographs, protein localization and abundance data:
- Type in the ORF or common name of the protein(s) of interest. Users are allowed to input more than one protein. Users can also paste in a list of proteins on the search box.
- Hit the search button.
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For details regarding CYCLoPs, click the About link
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For the user's guide, click the Help link
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To download files, click the Supplemental Files link
MICROGRAPHS
- Clicking the micrographs thumbnail opens up a modal which contains images from 21 screens.
- Each screen has 4 fields. Slide through different fields by doing
either of the following:
- click the left or right icon
- click the dots at the bottom of the image
- click and hold on the image then swipe left or right
- Toggle channel display by choosing either Overlay, GFP or RFP on the modal header. By default, the overlay image is displayed.
- For a closer look on the cells, enable the magnifying glass by clicking on the icon. To disable it, just click again on the icon.
- To adjust the image brightness, click on the icon and use the slider to increase or decrease the brightness.
SUB-CELLULAR LOCALIZATION
- Displays the LOC-score (quantitative measurement of localization) of the protein in each screen.
- The LOC-score is calculated from the proportion of cells that exhibit morphology specific to a localization class.
- If the column is left blank, it means than insufficient cells were obtained in the screen to make a definitive measurement of localization changes.
- Toggle between LOC-score and Cell count to see percentage vs raw data.
YEAST-GFP LOCALIZATION
- Shows the localization assignment determined manually by 2 annotators in previous study - W.-K. Huh, J.V. Falvo, L.C. Gerke, A.S. Carroll, R.W. Howson, J.S. Weissman & E.K. O'Shea, Global Analysis of Protein Localization in Budding Yeast, Nature, 425, 686-691 (2003).
PROTEIN ABUNDANCE
- Displays the GFP intensity measures of the protein in each screen as a proxy of protein abundance.
- Click the Plot time-course intensity button to open up a collapsible element which shows subplots of intensity levels across time-points for each of the treatment conditions: rapamycin, hydroxy-urea, rpd3 knockout and alpha-factor. Hide the plots by clicking on the button again.
LOCALIZATION CHANGE
- The z-LOC scores quantify changes in sub-cellular compartments with respect to wildtype LOC-scores.
- A negative z-LOC value denotes the exit of a protein from a particular compartment while a positive z-LOC value represents movement towards a specific location.