Standard name
Description Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23; other members of the complex are Spc1p, Spc2p, and Sec11p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.07
Bud 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.05 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0.09 0.13 0 0 0 0 0 0
Cytoplasm 0.22 0.33 0.36 0.33 0.33 0.32 0.36 0.32 0.28 0.31 0.34 0.33 0.37 0.3 0.05 0.1 0.06 0.12 0.07 0.09
Endoplasmic Reticulum 0.68 0.6 0.49 0.23 0.26 0.32 0.13 0.11 0.17 0.08 0.15 0.6 0.55 0.63 0.79 0.68 0.73 0.55 0.59 0.55
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.21 0.52 0.43 0.46 0.51 0.53 0.48 0.59 0.57 0 0 0 0.05 0.07 0.07 0.1 0.11 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.09 0 0.06 0.17 0.09 0.17 0.2 0.21 0.17 0.09 0 0.05 0 0 0 0 0.09 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 5 7 9 5 9 5 7 6 4 7 3 2 6 4 7 8 1 3 6
Bud 2 1 2 12 8 10 11 15 14 21 17 0 0 1 2 6 1 3 4 4
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 2 0 0
Bud Site 0 0 0 0 0 1 0 0 2 1 0 0 0 0
Cell Periphery 4 5 4 14 10 11 14 15 17 18 24 2 7 21 1 1 4 1 1 2
Cytoplasm 61 52 59 107 102 87 142 160 110 100 145 16 28 50 14 17 12 8 6 8
Endoplasmic Reticulum 185 96 80 75 80 87 53 56 70 25 64 29 41 106 247 120 143 42 58 53
Endosome 5 0 0 6 8 1 5 9 3 3 0 0 0 1 3 0 1 0 1 0
Golgi 2 1 0 1 0 0 0 0 0 0 0 0 0 2 4 0 0 0 0 3
Mitochondria 11 2 35 171 135 124 204 265 191 192 244 0 0 0 16 13 13 7 10 10
Nucleus 0 0 0 1 1 2 3 10 9 7 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 1 2 2 2 1 3 7 2 4 2 8 0 0 4 0 0 0 0 1 0
Nucleolus 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 0 1
Vac/Vac Membrane 34 14 5 21 53 25 66 98 84 56 39 2 4 1 13 4 5 6 7 4
Unique Cell Count 272 160 164 329 312 271 397 501 400 327 429 48 75 167 313 178 197 77 99 97
Labelled Cell Count 311 179 194 419 403 361 510 637 511 429 549 52 82 194 313 178 197 77 99 97


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 7.1 5.3 5.5 5.7 5.6 5.3 5.4 5.1 5.1 5.3 8.3 7.9 8.1 8.4 8.7 8.8
Std Deviation (1e-4) 1.7 1.7 1.3 1.5 1.8 1.5 1.6 1.5 1.3 1.5 1.4 2.6 2.2 1.7 2.4 2.6 2.0
Intensity Change (Log2) 0.03 0.1 0.06 -0.02 0.0 -0.07 -0.07 -0.02 0.64 0.57 0.59 0.65 0.71 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 0 -0.5 0 0 0 0 0 0 0 -0.3
Bud 0 0 0 0 0 0 2.6 1.7 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 0 0.9 0.7 0 1.0 1.5 1.6 0 0 3.5
Cytoplasm -0.8 -0.7 -0.8 0 -1.0 -2.0 -1.2 -0.5 -0.3 0.2 -1.2
Endoplasmic Reticulum -5.9 -5.1 -3.5 -9.0 -10.4 -7.6 -10.5 -8.6 1.4 0.8 2.7
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.5 4.7 5.1 6.5 7.0 5.8 7.8 7.8 -3.5 -4.3 -6.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.6 4.4 2.5 4.4 5.1 5.3 4.5 2.5 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available