Standard name
Description Mitochondrial elongation factor involved in translational elongation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.06 0.1 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.05 0 0.06 0.08 0.08 0 0.09 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.98 0.97 0.88 0.92 0.8 0.7 0.63 0.66 0.67 0.98 0.97 0.97 0.96 0.95 0.99 0.97 0.95 0.79 0.81 0.79
Endoplasmic Reticulum 0 0 0 0 0.13 0.05 0.12 0.16 0.14 0.17 0.16 0 0 0 0 0 0 0 0 0.13 0.12 0.15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.07 0.13 0.13 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 4 13 23 14 12 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2 8 5 14 3 12 17 20 8 16 1 2 2 8 4 0 0 0 0 0 0
Cytoplasm 169 317 263 211 231 133 161 150 149 117 126 255 292 319 358 212 165 305 260 165 199 232
Endoplasmic Reticulum 0 2 5 5 33 7 24 35 34 31 29 5 11 12 14 3 0 1 6 27 29 44
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 4 10 8 7 7 0 0 0 2 4 0 0 0 0 2 1
Mitochondria 0 0 0 0 0 3 0 16 30 23 12 0 0 0 5 0 0 0 0 3 2 1
Nucleus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 1 1 1 7 2 7 0 1 1 3 5 1 3 3 5 2 6
Unique Cell Count 169 318 268 218 263 144 200 214 237 178 187 260 301 328 371 222 168 314 274 211 245 295
Labelled Cell Count 169 321 276 222 278 148 206 242 272 204 209 261 306 334 391 228 168 314 274 211 245 295


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 64.8 75.8 49.8 45.5 40.8 33.8 29.6 27.7 23.3 23.1 24.1 59.1 58.3 54.1 63.1 66.3 54.5 52.8 57.7
Std Deviation (1e-4) 10.8 13.8 10.1 9.5 8.1 7.4 6.4 6.5 5.1 5.6 5.7 12.5 9.6 11.8 17.8 17.2 13.5 13.8 14.9
Intensity Change (Log2) -0.13 -0.29 -0.56 -0.75 -0.85 -1.1 -1.11 -1.05 0.24 0.23 0.12 0.34 0.41 0.13 0.08 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 4.1 5.2 4.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 1.4 0 1.6 2.4 2.7 0.8 2.6 0 0 0 -0.7 -0.8
Cytoplasm -1.0 -4.7 -2.9 -6.5 -8.7 -10.2 -9.4 -9.2 0 -0.9 -0.7 -1.2 -1.7
Endoplasmic Reticulum 0 4.8 0 4.5 5.7 5.2 5.9 5.4 0 1.3 1.3 1.4 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 4.6 6.0 6.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mef1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available