Standard name
Human Ortholog
Description Sterol transporter that transfers sterols between membranes; may regulate and coordinate formation of contact sites between organelles; localizes to ER-mitochondrial contact sites in a Tom70p- and Tom71p-dependent manner; mitochondrial localization requires GRAM domain; also localizes to ER-vacuole contact sites, in a Vac8p-dependent manner; has GRAM and StART-like (VASt) domains; one of six StART-like domain-containing proteins in yeast; conserved across eukaryotes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0.06 0 0.06 0.07
Bud 0 0.06 0 0.09 0.06 0.07 0.1 0.13 0.11 0.12 0.12 0.05 0 0.05 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0.13 0.11 0.13 0.08 0.1 0.06 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.15 0.42 0.57 0.4 0.38 0.22 0.16 0.09 0.1 0.08 0.09 0.47 0.5 0.49 0.25 0.24 0.22 0.05 0.11 0.07 0.09 0 0.05
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0.09 0 0 0 0 0.09 0.18 0.22 0.16 0.25 0.25 0.22 0.43 0.27 0.23 0.24 0.3 0.25
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.59 0.38 0 0.17 0.05 0.28 0.27 0.57 0.52 0.64 0.36 0.2 0.08 0.12 0.07 0.05 0.08 0.23 0.31 0.38 0.27 0.24 0.26
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.14 0.15 0.12 0 0.05 0.09 0 0.05 0.1 0.12 0.11
SpindlePole 0.24 0.12 0.24 0.32 0.44 0.4 0.56 0.36 0.42 0.32 0.5 0.16 0.16 0.18 0.38 0.42 0.38 0.1 0.09 0.09 0.09 0.12 0.13
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.08 0 0.08 0.05 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 7 14 8 12 4 20 0 3 4 7 5 0 5 5 12 12 6 9 21 15 33 46
Bud 6 9 9 19 19 23 57 47 56 52 63 17 18 33 6 4 10 0 0 2 1 8 7
Bud Neck 3 4 29 24 43 28 58 22 19 18 41 1 5 6 0 7 2 0 1 5 15 12 20
Bud Site 0 2 0 2 4 3 20 15 15 15 17 5 1 5 2 2 3
Cell Periphery 0 0 2 0 1 0 0 0 0 0 0 0 0 0 1 1 2 0 1 1 2 0 3
Cytoplasm 22 65 124 87 131 76 97 33 48 38 48 173 234 307 74 49 44 10 25 24 36 24 34
Endoplasmic Reticulum 6 13 5 1 2 5 1 0 0 2 3 4 12 10 15 5 7 0 1 1 2 5 8
Endosome 7 2 1 8 7 30 14 7 11 0 48 68 102 103 74 52 43 92 65 83 99 178 169
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 6 5 1 0 2 2 3 3
Mitochondria 85 58 8 37 17 97 157 206 262 290 198 74 35 76 21 11 15 49 74 139 111 141 172
Nucleus 2 1 4 2 0 4 10 6 10 10 5 1 0 2 2 4 2 0 0 0 1 0 0
Nuclear Periphery 1 1 0 0 1 3 1 0 1 0 4 0 0 1 0 0 3 2 2 0 0 1 0
Nucleolus 3 0 0 2 0 5 6 5 16 18 12 0 0 1 1 1 1 0 0 1 3 2 1
Peroxisomes 2 0 0 6 4 10 4 16 17 6 31 23 65 93 35 9 9 19 8 18 39 72 75
SpindlePole 35 19 51 69 149 135 332 131 212 143 274 60 76 111 110 87 75 20 21 34 38 74 90
Vac/Vac Membrane 6 6 5 3 8 3 2 8 5 6 14 11 13 8 25 14 15 4 19 17 26 21 27
Unique Cell Count 144 154 217 217 341 341 592 364 503 450 543 372 465 631 292 206 197 214 241 367 409 593 675
Labelled Cell Count 180 187 253 268 398 426 779 496 675 602 765 442 561 761 372 264 248 214 241 367 409 593 675


Mitochondria, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.6 4.8 4.3 4.9 3.9 4.1 3.7 3.6 3.6 4.1 3.9 3.9 3.9 6.3 6.5 6.5 3.7 3.9 4.0
Std Deviation (1e-4) 0.5 0.5 0.8 1.4 1.1 0.9 1.2 1.2 0.9 0.9 0.9 0.8 0.8 0.9 1.6 1.4 1.6 0.8 1.2 1.1
Intensity Change (Log2) -0.16 0.02 -0.29 -0.21 -0.37 -0.4 -0.42 -0.22 -0.28 -0.31 -0.29 0.41 0.45 0.45 -0.37 -0.31 -0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.0 2.2 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0046 -0.3613 -0.533 -0.1271 0.4547 -0.6388 -0.4492 -0.9054 -0.7717 -0.5936 -0.094 -0.8856 -0.032 -0.7634 -0.8512 0.1585 -1.3526 -0.8285
Actin 0.0158 0.0068 0.0135 0.0021 0.0639 0.0161 0.0374 0.0157 0.0137 0.0596 0.006 0.0279 0.0693 0.0067 0.0237 0.0613 0.0112 0.0162
Bud 0.0006 0.0024 0.0009 0.0007 0.0058 0.0013 0.0013 0.0003 0.0005 0.0021 0.0032 0.0003 0.0006 0.0005 0.0012 0.0015 0.0002 0.0002
Bud Neck 0.0065 0.0007 0.0081 0.0049 0.0024 0.0053 0.0037 0.0005 0.0116 0.0011 0.0007 0.0056 0.001 0.0014 0.0074 0.0033 0.0009 0.0071
Bud Periphery 0.0009 0.0014 0.0013 0.0018 0.0081 0.0021 0.0019 0.0006 0.0006 0.0037 0.0048 0.0004 0.0007 0.0006 0.0022 0.0023 0.0002 0.0002
Bud Site 0.008 0.0135 0.0055 0.0491 0.0365 0.0013 0.0251 0.0034 0.0046 0.0087 0.0161 0.0005 0.0022 0.013 0.0033 0.008 0.0003 0.0004
Cell Periphery 0.0004 0.0009 0.0006 0.0001 0.0005 0.0003 0.0011 0.0002 0.0002 0.0002 0.0003 0.0001 0.0002 0.0004 0.0005 0.0004 0.0001 0.0001
Cytoplasm 0.0208 0.0363 0.0185 0.0321 0.0058 0.0251 0.0376 0.018 0.0124 0.0216 0.0068 0.0308 0.017 0.0493 0.0137 0.0528 0.0345 0.0455
Cytoplasmic Foci 0.1718 0.2449 0.2549 0.1405 0.0967 0.2314 0.2036 0.2909 0.2899 0.3318 0.3292 0.2496 0.1862 0.2537 0.2713 0.2928 0.2882 0.2694
Eisosomes 0.0005 0.0009 0.0004 0.0001 0.0009 0.0005 0.0006 0.0006 0.0002 0.0004 0.0001 0.0001 0.0004 0.0007 0.0009 0.0005 0.0001 0.0001
Endoplasmic Reticulum 0.005 0.0042 0.0036 0.0048 0.0172 0.003 0.0075 0.0028 0.0048 0.0021 0.001 0.0025 0.0045 0.004 0.002 0.0018 0.0014 0.0068
Endosome 0.0689 0.1118 0.09 0.1366 0.0333 0.0605 0.0848 0.1023 0.1224 0.0723 0.1591 0.1008 0.1409 0.0995 0.1166 0.1522 0.0582 0.1462
Golgi 0.0222 0.0233 0.0181 0.0172 0.0172 0.0193 0.0272 0.0207 0.0382 0.0427 0.0942 0.0337 0.0391 0.0246 0.0385 0.0512 0.0349 0.0443
Lipid Particles 0.2355 0.181 0.1901 0.1664 0.1805 0.2261 0.2511 0.1729 0.1637 0.1212 0.1004 0.2208 0.2604 0.1388 0.1774 0.1225 0.1824 0.2014
Mitochondria 0.0103 0.0136 0.0202 0.0074 0.036 0.0149 0.0223 0.0053 0.0224 0.0174 0.0104 0.0068 0.0074 0.0083 0.0192 0.0151 0.0346 0.0105
None 0.1832 0.1921 0.0818 0.0844 0.0655 0.1135 0.1163 0.214 0.0825 0.1641 0.0994 0.0647 0.0481 0.2453 0.0947 0.0212 0.0904 0.0526
Nuclear Periphery 0.0208 0.0072 0.0112 0.0182 0.0462 0.0051 0.0128 0.0016 0.013 0.0012 0.0008 0.0024 0.0192 0.0019 0.0011 0.0007 0.0007 0.0029
Nucleolus 0.0013 0.002 0.0046 0.0021 0.0288 0.0011 0.009 0.0013 0.001 0.0007 0.0013 0.0002 0.0014 0.0014 0.0009 0.0005 0.0001 0.0004
Nucleus 0.0082 0.0054 0.0126 0.0583 0.0304 0.0018 0.0139 0.0026 0.0024 0.0004 0.0008 0.0009 0.0077 0.0027 0.0006 0.0008 0.0001 0.0009
Peroxisomes 0.1937 0.1317 0.226 0.2497 0.3073 0.2516 0.1154 0.1332 0.1916 0.1392 0.1559 0.2414 0.1513 0.133 0.214 0.1919 0.2557 0.1761
Punctate Nuclear 0.0141 0.0108 0.0295 0.0134 0.0122 0.0156 0.02 0.009 0.0156 0.0068 0.0048 0.0058 0.0374 0.0088 0.0067 0.0097 0.0035 0.0127
Vacuole 0.0082 0.0075 0.0066 0.0066 0.0022 0.0027 0.0052 0.0031 0.0057 0.002 0.0035 0.0034 0.003 0.0046 0.0033 0.0078 0.0015 0.0047
Vacuole Periphery 0.0036 0.0016 0.0019 0.0034 0.0025 0.0013 0.002 0.001 0.0029 0.0008 0.0013 0.0012 0.0018 0.0009 0.001 0.0017 0.0008 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2146 5.6852 13.7106 14.1888 13.3991 10.5936 10.4187 14.3872 18.564 14.6797
Translational Efficiency 0.8731 1.1888 0.6598 0.7254 0.5968 0.8091 0.8716 0.6258 0.6202 0.6302

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1689 1738 202 823 1679 1506 2099 1499 3368 3244 2301 2322

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 597.77 735.98 897.36 836.66 659.99 724.02 798.44 816.78 628.79 730.43 807.12 823.83
Standard Deviation 67.62 91.68 86.50 107.62 71.02 91.94 93.23 106.29 76.00 91.99 96.80 107.18
Intensity Change Log 2 0.300076 0.586096 0.485051 0.133585 0.274740 0.307503 0.215112 0.431109 0.394614

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000250 0.002168 0.016742 0.054033 0.000798 0.001613 0.004577 0.049032 0.000523 0.001910 0.005645 0.050804
Bud Neck 0.045848 0.029922 0.011511 0.013942 0.019070 0.033603 0.018542 0.013960 0.032499 0.031631 0.017925 0.013954
Bud Site 0.005924 0.012732 0.034935 0.054150 0.003607 0.009963 0.018464 0.053113 0.004769 0.011446 0.019910 0.053481
Cell Periphery 0.000171 0.000097 0.000458 0.000226 0.000133 0.000210 0.000150 0.000251 0.000152 0.000149 0.000177 0.000242
Cytoplasm 0.159341* 0.063304 0.063631 0.058229 0.039552 0.066214 0.060761 0.057929 0.099624 0.064655 0.061013 0.058035
Cytoplasmic Foci 0.405676* 0.358897* 0.154148 0.177688 0.349275* 0.348089* 0.226083* 0.185880* 0.377559* 0.353880* 0.219768* 0.182976*
Eisosomes 0.000361 0.000201 0.000293 0.000258 0.000300 0.000361 0.000135 0.000299 0.000331 0.000275 0.000149 0.000284
Endoplasmic Reticulum 0.001140 0.000737 0.020672 0.002765 0.000769 0.000885 0.001398 0.002318 0.000955 0.000806 0.003090 0.002477
Endosome 0.073169 0.123035 0.058704 0.057456 0.120800 0.128330 0.118811 0.063825 0.096914 0.125493 0.113534 0.061568
Golgi 0.018271 0.053375 0.004252 0.012665 0.058999 0.061533 0.009923 0.015080 0.038574 0.057162 0.009425 0.014224
Lipid Particles 0.093221 0.047102 0.063440 0.022144 0.156007* 0.072927 0.068997 0.028659 0.124521* 0.059091 0.068509 0.026350
Mitochondria 0.031073 0.044941 0.001491 0.003188 0.031297 0.074904 0.007251 0.005600 0.031185 0.058851 0.006745 0.004745
Mitotic Spindle 0.004034 0.032743 0.102390* 0.088969 0.009783 0.013792 0.087406 0.069471 0.006900 0.023945 0.088721 0.076382
None 0.006382 0.000657 0.000954 0.000819 0.001233 0.000837 0.000908 0.000266 0.003815 0.000740 0.000912 0.000462
Nuclear Periphery 0.000856 0.000826 0.003654 0.000462 0.000523 0.000546 0.001583 0.000355 0.000690 0.000696 0.001764 0.000393
Nuclear Periphery Foci 0.001272 0.001839 0.037789 0.003336 0.001655 0.001034 0.006465 0.002682 0.001463 0.001465 0.009215 0.002914
Nucleolus 0.007753 0.003556 0.005751 0.000901 0.004249 0.003114 0.004726 0.000903 0.006006 0.003350 0.004816 0.000902
Nucleus 0.062613 0.018470 0.168762* 0.013952 0.012561 0.019017 0.032826 0.016481 0.037661 0.018724 0.044760 0.015585
Peroxisomes 0.057585 0.167285* 0.151486* 0.356286* 0.176149* 0.126200* 0.273161* 0.375541* 0.116691* 0.148211* 0.262479* 0.368717*
Vacuole 0.023018 0.035482 0.098185 0.077511 0.011736 0.032269 0.056727 0.057332 0.017394 0.033990 0.060367 0.064484
Vacuole Periphery 0.002040 0.002631 0.000753 0.001019 0.001504 0.004560 0.001106 0.001024 0.001773 0.003527 0.001075 0.001022

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.12 -5.79 -10.94 -10.46 -6.21 -2.74 -7.85 -14.21 -13.88 -13.00 -6.34 -10.22 -17.97 -17.35 -15.84
Bud Neck 4.57 10.55 10.09 6.62 -0.43 -5.01 -0.62 1.95 6.95 3.26 0.24 6.67 9.51 9.63 3.60
Bud Site -5.14 -7.26 -12.47 -10.57 -3.06 -5.97 -8.92 -15.21 -12.76 -9.86 -7.80 -9.71 -19.38 -16.29 -11.88
Cell Periphery 6.84 -7.62 -3.74 -7.58 5.55 -2.56 -0.95 -5.12 -1.96 -4.95 0.21 -2.17 -6.43 -6.47 -3.83
Cytoplasm 15.39 9.47 12.36 -1.04 -0.20 -6.54 -7.35 -6.94 -0.50 0.19 8.63 6.55 6.96 -1.14 0.09
Cytoplasmic Foci 6.65 22.55 28.22 23.97 -2.95 0.32 19.36 24.40 23.35 6.93 4.90 30.06 37.59 34.03 7.20
Eisosomes 4.27 0.58 2.25 -3.24 2.23 -1.61 7.17 -0.40 1.32 -7.94 2.01 8.18 1.27 -0.96 -8.93
Endoplasmic Reticulum 2.04 -9.71 -6.51 -7.53 8.28 -0.78 -6.21 -8.55 -7.97 -3.28 0.98 -9.93 -10.29 -11.24 1.67
Endosome -10.01 2.89 4.61 13.26 0.32 -1.30 2.08 11.54 12.71 10.35 -7.28 -1.75 11.18 18.14 11.56
Golgi -13.47 11.90 4.94 16.46 -4.58 -0.68 19.24 16.82 16.14 -3.97 -7.85 19.97 16.38 23.02 -4.17
Lipid Particles 9.81 3.98 15.54 6.78 4.41 12.49 14.61 21.73 10.12 10.40 16.03 13.61 26.09 11.56 12.26
Mitochondria -4.27 13.68 12.64 16.78 -1.91 -9.76 11.29 12.26 17.48 2.73 -10.18 15.53 17.35 23.64 3.78
Mitotic Spindle -10.46 -6.21 -11.54 -7.22 0.33 -1.82 -17.50 -12.96 -12.08 2.66 -9.55 -19.53 -17.64 -12.85 2.15
None 6.16 5.52 5.34 -0.11 0.63 1.49 1.11 2.93 1.61 3.41 6.23 5.68 6.43 0.96 2.13
Nuclear Periphery -0.26 -6.12 0.41 0.53 6.19 -0.22 -7.29 -1.23 -0.92 6.97 -0.39 -7.68 0.05 0.43 7.72
Nuclear Periphery Foci -1.33 -6.74 -6.10 -4.83 6.03 2.45 -6.14 -2.62 -5.26 3.84 0.83 -9.49 -5.52 -6.47 6.21
Nucleolus 3.49 0.21 6.83 3.20 1.87 1.63 -1.96 3.44 1.91 6.01 3.71 -0.43 7.13 3.35 6.94
Nucleus 13.68 -6.53 10.96 -1.14 8.61 -3.33 -10.61 -5.22 -2.10 5.27 9.34 -5.22 5.99 -2.42 9.14
Peroxisomes -18.65 -4.60 -20.76 -10.44 -9.62 6.85 -7.74 -15.56 -21.22 -8.78 -6.56 -17.10 -27.30 -22.38 -10.07
Vacuole -6.31 -9.83 -14.59 -12.38 -0.92 -10.17 -20.64 -17.69 -12.14 -2.46 -11.41 -21.04 -22.26 -17.03 -5.06
Vacuole Periphery -1.95 6.43 4.22 5.71 -0.54 -6.37 2.68 2.23 7.17 0.22 -6.37 4.61 4.33 8.88 0.29