Standard name
Description Sterol transporter that transfers sterols between membranes; may regulate and coordinate formation of contact sites between organelles; localizes to ER-mitochondrial contact sites in a Tom70p- and Tom71p-dependent manner; mitochondrial localization requires GRAM domain; also localizes to ER-vacuole contact sites, in a Vac8p-dependent manner; has GRAM and StART-like (VASt) domains; one of six StART-like domain-containing proteins in yeast; conserved across eukaryotes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0.06 0 0.06 0.07
Bud 0 0.06 0 0.09 0.06 0.07 0.1 – 0.13 0.11 0.12 0.12 0.05 0 0.05 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0.13 0.11 0.13 0.08 0.1 – 0.06 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.15 0.42 0.57 0.4 0.38 0.22 0.16 – 0.09 0.1 0.08 0.09 0.47 0.5 0.49 0.25 0.24 0.22 0.05 0.11 0.07 0.09 0 0.05
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0.09 0 – 0 0 0 0.09 0.18 0.22 0.16 0.25 0.25 0.22 0.43 0.27 0.23 0.24 0.3 0.25
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.59 0.38 0 0.17 0.05 0.28 0.27 – 0.57 0.52 0.64 0.36 0.2 0.08 0.12 0.07 0.05 0.08 0.23 0.31 0.38 0.27 0.24 0.26
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0.06 0.06 0.14 0.15 0.12 0 0.05 0.09 0 0.05 0.1 0.12 0.11
SpindlePole 0.24 0.12 0.24 0.32 0.44 0.4 0.56 – 0.36 0.42 0.32 0.5 0.16 0.16 0.18 0.38 0.42 0.38 0.1 0.09 0.09 0.09 0.12 0.13
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.07 0.08 0 0.08 0.05 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 7 14 8 12 4 20 – 0 3 4 7 5 0 5 5 12 12 6 9 21 15 33 46
Bud 6 9 9 19 19 23 57 – 47 56 52 63 17 18 33 6 4 10 0 0 2 1 8 7
Bud Neck 3 4 29 24 43 28 58 – 22 19 18 41 1 5 6 0 7 2 0 1 5 15 12 20
Bud Site 0 2 0 2 4 3 20 – 15 15 15 17 5 1 5 2 2 3 – – – – – –
Cell Periphery 0 0 2 0 1 0 0 – 0 0 0 0 0 0 0 1 1 2 0 1 1 2 0 3
Cytoplasm 22 65 124 87 131 76 97 – 33 48 38 48 173 234 307 74 49 44 10 25 24 36 24 34
Endoplasmic Reticulum 6 13 5 1 2 5 1 – 0 0 2 3 4 12 10 15 5 7 0 1 1 2 5 8
Endosome 7 2 1 8 7 30 14 – 7 11 0 48 68 102 103 74 52 43 92 65 83 99 178 169
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 1 6 5 1 0 2 2 3 3
Mitochondria 85 58 8 37 17 97 157 – 206 262 290 198 74 35 76 21 11 15 49 74 139 111 141 172
Nucleus 2 1 4 2 0 4 10 – 6 10 10 5 1 0 2 2 4 2 0 0 0 1 0 0
Nuclear Periphery 1 1 0 0 1 3 1 – 0 1 0 4 0 0 1 0 0 3 2 2 0 0 1 0
Nucleolus 3 0 0 2 0 5 6 – 5 16 18 12 0 0 1 1 1 1 0 0 1 3 2 1
Peroxisomes 2 0 0 6 4 10 4 – 16 17 6 31 23 65 93 35 9 9 19 8 18 39 72 75
SpindlePole 35 19 51 69 149 135 332 – 131 212 143 274 60 76 111 110 87 75 20 21 34 38 74 90
Vac/Vac Membrane 6 6 5 3 8 3 2 – 8 5 6 14 11 13 8 25 14 15 4 19 17 26 21 27
Unique Cell Count 144 154 217 217 341 341 592 364 503 450 543 372 465 631 292 206 197 214 241 367 409 593 675
Labelled Cell Count 180 187 253 268 398 426 779 496 675 602 765 442 561 761 372 264 248 214 241 367 409 593 675


Mitochondria, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.6 4.8 4.3 4.9 3.9 4.1 – 3.7 3.6 3.6 4.1 3.9 3.9 3.9 6.3 6.5 6.5 3.7 3.9 4.0
Std Deviation (1e-4) 0.5 0.5 0.8 1.4 1.1 0.9 1.2 – 1.2 0.9 0.9 0.9 0.8 0.8 0.9 1.6 1.4 1.6 0.8 1.2 1.1
Intensity Change (Log2) – – – -0.16 0.02 -0.29 -0.21 – -0.37 -0.4 -0.42 -0.22 -0.28 -0.31 -0.29 0.41 0.45 0.45 -0.37 -0.31 -0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.0 2.2 2.5