Standard name
Human Ortholog
Description C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0.05 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.08 0.07 – 0.07 0.05 0 0.06 0.06 0.11 0.14 0.15 0.08 0.15 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.07 0.07 0.31 – 0.29 0.38 0.38 0.39 0.47 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.96 0.96 0.89 0.82 0.77 – 0.72 0.71 0.74 0.74 0.7 0.85 0.76 0.68 0.84 0.85 0.81 0.94 0.92 0.95 0.8 0.69 0.57
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0.05 0.06 – 0.1 0.07 0.08 0.11 0.06 0 0.12 0.18 0 0 0 0 0 0 0 0.07 0.09
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0.13 0.19 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0
Bud 0 0 0 0 2 1 – 3 3 3 2 4 1 0 0 1 0 0 7 3 0 1 6 1
Bud Neck 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 6 8 – 14 3 21 13 20 1 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 9 4 11 28 24 – 45 16 11 24 27 35 45 58 13 5 18 1 2 0 1 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 2 0 1 0 0 2 1 0 0
Endosome 0 0 0 0 1 0 – 1 0 1 0 0 1 1 7 0 0 0 3 1 1 2 1 1
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 1 0 2 0 2 1
Mitochondria 8 0 13 15 24 106 – 184 133 150 157 201 3 1 2 0 1 1 8 0 2 0 2 2
Nucleus 910 439 242 191 272 267 – 448 247 292 297 298 269 250 258 132 29 106 916 434 246 259 258 170
Nuclear Periphery 0 1 1 0 2 3 – 0 0 2 5 12 0 4 1 0 0 1 0 0 0 2 0 3
Nucleolus 19 5 4 15 17 22 – 64 26 32 44 26 10 40 70 5 0 4 3 4 1 8 26 27
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 3 6 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 – 3 0 0 1 0 3 5 9 0 0 0 2 0 0 0 0 1
Vac/Vac Membrane 16 8 0 4 10 14 – 25 12 20 5 4 1 4 8 8 1 3 25 20 3 40 69 82
Unique Cell Count 944 457 252 214 330 347 624 348 393 401 427 318 331 381 157 34 131 971 474 260 325 374 300
Labelled Cell Count 961 462 264 236 364 445 788 441 532 548 593 324 355 420 162 36 134 971 474 260 325 374 300


Endoplasmic Reticulum, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.8 19.0 8.3 9.4 7.7 5.6 – 5.4 4.9 5.2 5.1 5.1 15.5 15.0 13.3 27.9 20.1 23.1 19.2 17.8 18.0
Std Deviation (1e-4) 3.7 5.4 2.0 2.4 1.9 1.3 – 1.3 1.1 1.3 1.2 1.4 5.6 5.4 4.7 9.9 7.9 7.9 5.4 4.5 4.7
Intensity Change (Log2) – – – 0.18 -0.11 -0.57 – -0.62 -0.76 -0.69 -0.72 -0.71 0.9 0.86 0.68 1.75 1.28 1.48 1.21 1.1 1.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 3.7 2.9 3.5 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 3.6 3.1 – 3.3 2.0 1.0 2.7 2.9 4.4 5.2 5.6 3.3 0 4.8
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 1.0 7.7 – 7.8 9.3 9.4 9.6 11.4 -3.0 -3.8 -3.8 0 0 0
Nucleus -2.8 -5.1 -6.5 – -7.9 -7.8 -7.1 -7.2 -8.2 -4.5 -6.8 -8.6 -4.2 -2.6 -4.9
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.9 2.3 2.8 – 4.3 3.3 3.5 4.5 2.8 1.2 4.8 6.4 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.2 – 3.2 3.0 3.6 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.5336 16.2114 15.0604 14.1507 13.5997 14.3613 12.8753 13.9774 13.2299 12.1832 10.9145 12.6748 16.4708 16.3583 15.6972 14.9763 14.5728 15.1464
Actin 0.0125 0 0 0 0 0 0.0015 0 0 0 0.0205 0.0001 0.0073 0 0 0.0056 0 0
Bud 0.0002 0 0 0 0 0 0.0004 0 0.0001 0 0.001 0 0.0002 0 0 0 0 0.0001
Bud Neck 0.0003 0 0 0 0.0001 0.0002 0.0005 0.0001 0 0 0.0019 0.0004 0.0009 0 0 0.0084 0 0.0003
Bud Periphery 0.0002 0 0 0 0.0001 0.0001 0.0008 0 0.0002 0 0.0018 0.0001 0.0003 0 0 0.0001 0 0.0001
Bud Site 0.0004 0 0 0 0 0 0.0013 0 0.0001 0 0.0037 0 0.0023 0 0 0.0003 0 0
Cell Periphery 0.0003 0 0 0 0.0001 0.0001 0.0008 0 0.0003 0 0.001 0.0001 0.0002 0 0 0.0002 0 0.0001
Cytoplasm 0.001 0 0 0 0 0 0.0009 0.0001 0.0002 0 0.0047 0 0.0012 0 0 0.001 0 0
Cytoplasmic Foci 0.0047 0 0 0 0 0 0.0093 0.0001 0.0003 0 0.0153 0.0006 0.0023 0 0 0.0007 0 0
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0002 0 0.0001 0 0 0.0008 0 0
Endoplasmic Reticulum 0.0005 0 0 0 0 0 0.0007 0 0.0002 0 0.0019 0 0.0007 0 0 0.0004 0 0
Endosome 0.0011 0 0 0 0 0 0.0043 0 0.0008 0 0.0123 0.0001 0.0026 0 0 0.0009 0 0
Golgi 0.0007 0 0 0 0 0 0.0017 0 0.0001 0 0.0184 0.0001 0.0015 0 0 0.0005 0 0
Lipid Particles 0.0029 0 0 0 0 0 0.0117 0.0001 0.0012 0 0.0155 0.0035 0.0032 0 0 0.001 0 0
Mitochondria 0.0037 0.0002 0.0002 0.0001 0.0004 0.0002 0.0068 0.0002 0.0011 0 0.0173 0.0009 0.0016 0.0002 0.0001 0.0002 0.0002 0.0004
None 0.0008 0 0 0 0 0 0.0007 0.0001 0.0003 0 0.0007 0.0001 0.0008 0 0 0.0055 0 0
Nuclear Periphery 0.0038 0.0011 0.0006 0.0004 0.0006 0.0003 0.009 0.0022 0.0036 0.0016 0.0139 0.0006 0.0033 0.0008 0.0006 0.0029 0.0004 0.0004
Nucleolus 0.0301 0.0058 0.0048 0.0028 0.0419 0.0212 0.0371 0.0153 0.0189 0.003 0.0723 0.0484 0.0069 0.0061 0.0065 0.0043 0.0286 0.0352
Nucleus 0.9285 0.9928 0.9942 0.9966 0.955 0.9777 0.8907 0.9803 0.9651 0.9952 0.7722 0.9438 0.9516 0.9928 0.9927 0.963 0.9706 0.9626
Peroxisomes 0.0003 0 0 0 0 0 0.009 0 0.0001 0 0.0027 0.0001 0.0112 0 0 0.0006 0 0
Punctate Nuclear 0.0045 0 0 0 0.0011 0 0.0062 0.0013 0.0013 0 0.0157 0.0005 0.0006 0 0.0001 0.0033 0.0001 0.0001
Vacuole 0.0014 0 0 0 0.0004 0.0002 0.0034 0.0001 0.0036 0 0.0043 0.0003 0.0009 0 0 0.0001 0 0.0003
Vacuole Periphery 0.0022 0 0.0001 0 0.0003 0.0001 0.0029 0.0001 0.0027 0 0.0028 0.0002 0.0005 0.0001 0 0.0001 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.2385 37.4075 46.7503 50.2581 75.6711 35.219 43.7947 48.3684 48.3918 46.9534
Translational Efficiency 5.9902 5.9596 4.1203 2.8904 2.4413 4.2128 2.6956 2.4854 1.9038 2.8979

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Bud20

Bud20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available