Standard name
Description C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0.05 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.08 0.07 – 0.07 0.05 0 0.06 0.06 0.11 0.14 0.15 0.08 0.15 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.07 0.07 0.31 – 0.29 0.38 0.38 0.39 0.47 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.96 0.96 0.89 0.82 0.77 – 0.72 0.71 0.74 0.74 0.7 0.85 0.76 0.68 0.84 0.85 0.81 0.94 0.92 0.95 0.8 0.69 0.57
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0.05 0.06 – 0.1 0.07 0.08 0.11 0.06 0 0.12 0.18 0 0 0 0 0 0 0 0.07 0.09
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0.13 0.19 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0
Bud 0 0 0 0 2 1 – 3 3 3 2 4 1 0 0 1 0 0 7 3 0 1 6 1
Bud Neck 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 6 8 – 14 3 21 13 20 1 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 9 4 11 28 24 – 45 16 11 24 27 35 45 58 13 5 18 1 2 0 1 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 2 0 1 0 0 2 1 0 0
Endosome 0 0 0 0 1 0 – 1 0 1 0 0 1 1 7 0 0 0 3 1 1 2 1 1
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 1 0 2 0 2 1
Mitochondria 8 0 13 15 24 106 – 184 133 150 157 201 3 1 2 0 1 1 8 0 2 0 2 2
Nucleus 910 439 242 191 272 267 – 448 247 292 297 298 269 250 258 132 29 106 916 434 246 259 258 170
Nuclear Periphery 0 1 1 0 2 3 – 0 0 2 5 12 0 4 1 0 0 1 0 0 0 2 0 3
Nucleolus 19 5 4 15 17 22 – 64 26 32 44 26 10 40 70 5 0 4 3 4 1 8 26 27
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 3 6 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 – 3 0 0 1 0 3 5 9 0 0 0 2 0 0 0 0 1
Vac/Vac Membrane 16 8 0 4 10 14 – 25 12 20 5 4 1 4 8 8 1 3 25 20 3 40 69 82
Unique Cell Count 944 457 252 214 330 347 624 348 393 401 427 318 331 381 157 34 131 971 474 260 325 374 300
Labelled Cell Count 961 462 264 236 364 445 788 441 532 548 593 324 355 420 162 36 134 971 474 260 325 374 300


Endoplasmic Reticulum, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.8 19.0 8.3 9.4 7.7 5.6 – 5.4 4.9 5.2 5.1 5.1 15.5 15.0 13.3 27.9 20.1 23.1 19.2 17.8 18.0
Std Deviation (1e-4) 3.7 5.4 2.0 2.4 1.9 1.3 – 1.3 1.1 1.3 1.2 1.4 5.6 5.4 4.7 9.9 7.9 7.9 5.4 4.5 4.7
Intensity Change (Log2) – – – 0.18 -0.11 -0.57 – -0.62 -0.76 -0.69 -0.72 -0.71 0.9 0.86 0.68 1.75 1.28 1.48 1.21 1.1 1.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 3.7 2.9 3.5 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 3.6 3.1 – 3.3 2.0 1.0 2.7 2.9 4.4 5.2 5.6 3.3 0 4.8
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 1.0 7.7 – 7.8 9.3 9.4 9.6 11.4 -3.0 -3.8 -3.8 0 0 0
Nucleus -2.8 -5.1 -6.5 – -7.9 -7.8 -7.1 -7.2 -8.2 -4.5 -6.8 -8.6 -4.2 -2.6 -4.9
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.9 2.3 2.8 – 4.3 3.3 3.5 4.5 2.8 1.2 4.8 6.4 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.2 – 3.2 3.0 3.6 0 0 0 0 0 0 0 0