Standard name
Description Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.82 0.93 0.9 0.86 0.66 0.47 0.45 0.42 0.42 0.36 0.33 0.43 0.96 0.97 0.98 0.91 0.96 0.91 0.63 0.73 0.68 0.84 0.73 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.18 0.21 0.25 0.65 0.64 0.66 0.75 0.76 0.8 0.73 0 0 0 0.09 0 0.07 0.09 0 0.15 0.05 0.11 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.07 0 0.06 0.1 0.09 0.1 0.07 0.05 0.06 0.08 0 0 0 0 0.06 0 0 0.08 0.08 0.06 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 1 0 3 5 10
Bud 6 0 1 3 2 6 6 11 4 9 12 13 5 1 3 0 0 1 2 1 0 2 3 19
Bud Neck 0 0 0 0 0 0 0 0 2 1 0 1 0 0 0 1 1 0 1 1 0 1 3 10
Bud Site 0 0 0 0 0 1 1 1 2 1 2 6 0 0 0 0 0 0
Cell Periphery 2 0 1 1 1 0 3 1 2 2 0 3 0 2 4 4 0 1 1 0 0 0 1 3
Cytoplasm 239 130 95 134 151 132 164 175 123 142 104 183 464 732 814 258 115 115 217 115 83 289 391 382
Endoplasmic Reticulum 4 1 0 0 5 3 1 6 3 0 2 2 7 5 6 16 2 5 5 1 5 5 8 21
Endosome 5 0 0 1 13 3 5 3 0 0 0 0 2 2 1 4 2 4 31 7 1 4 20 23
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 3 1 0 3 2 5
Mitochondria 35 6 19 32 58 184 232 274 221 301 251 307 3 4 0 25 4 9 29 4 18 16 59 72
Nucleus 0 0 0 0 2 2 4 5 7 8 8 11 3 0 2 0 1 1 2 8 0 1 1 1
Nuclear Periphery 2 1 1 0 0 0 2 3 0 5 4 3 0 0 0 0 0 1 2 0 1 0 2 2
Nucleolus 1 0 0 0 0 0 2 0 0 2 0 2 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 4 4 2 3 6 8 0 0 0 1 0 0 8 0 0 4 9 15
Vac/Vac Membrane 19 10 4 9 23 26 35 31 15 25 24 17 13 23 19 16 3 4 28 11 7 6 16 46
Unique Cell Count 290 140 106 156 228 281 361 415 295 397 315 422 485 752 833 282 120 127 347 157 123 346 534 628
Labelled Cell Count 313 148 121 180 255 358 459 514 381 499 413 556 497 770 851 326 129 141 347 157 123 346 534 628


Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.8 4.4 4.5 4.4 3.7 3.3 3.5 3.3 3.1 3.1 3.0 5.9 5.8 5.8 5.0 5.9 6.3 5.7 5.6 6.2
Std Deviation (1e-4) 0.7 1.0 0.7 1.1 1.1 1.1 1.1 0.7 0.9 1.0 1.0 1.2 1.0 1.2 1.3 1.2 1.5 1.2 1.8 1.3 1.4
Intensity Change (Log2) 0.03 -0.0 -0.23 -0.41 -0.34 -0.39 -0.48 -0.48 -0.55 0.44 0.42 0.41 0.2 0.43 0.52 0.38 0.35 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -4.5 -7.6 -8.0 -8.7 -8.5 -9.9 -10.1 -8.5 2.5 4.0 4.5 0.6 1.8 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -2.5 -3.6 -2.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 2.0 1.8 1.9 1.4 0.5 1.0 1.4 0 0 0 0 0.8 0 0