Standard name | |
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Description | Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 3 | 5 | 10 |
Bud | 6 | 0 | 1 | 3 | 2 | 6 | 6 | 11 | 4 | 9 | 12 | 13 | 5 | 1 | 3 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 3 | 19 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 3 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 1 | 1 | 1 | 0 | 3 | 1 | 2 | 2 | 0 | 3 | 0 | 2 | 4 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 3 |
Cytoplasm | 239 | 130 | 95 | 134 | 151 | 132 | 164 | 175 | 123 | 142 | 104 | 183 | 464 | 732 | 814 | 258 | 115 | 115 | 217 | 115 | 83 | 289 | 391 | 382 |
Endoplasmic Reticulum | 4 | 1 | 0 | 0 | 5 | 3 | 1 | 6 | 3 | 0 | 2 | 2 | 7 | 5 | 6 | 16 | 2 | 5 | 5 | 1 | 5 | 5 | 8 | 21 |
Endosome | 5 | 0 | 0 | 1 | 13 | 3 | 5 | 3 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 4 | 2 | 4 | 31 | 7 | 1 | 4 | 20 | 23 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 3 | 2 | 5 |
Mitochondria | 35 | 6 | 19 | 32 | 58 | 184 | 232 | 274 | 221 | 301 | 251 | 307 | 3 | 4 | 0 | 25 | 4 | 9 | 29 | 4 | 18 | 16 | 59 | 72 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 5 | 7 | 8 | 8 | 11 | 3 | 0 | 2 | 0 | 1 | 1 | 2 | 8 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 2 | 1 | 1 | 0 | 0 | 0 | 2 | 3 | 0 | 5 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 2 | 2 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 2 | 3 | 6 | 8 | 0 | 0 | 0 | 1 | 0 | 0 | 8 | 0 | 0 | 4 | 9 | 15 |
Vac/Vac Membrane | 19 | 10 | 4 | 9 | 23 | 26 | 35 | 31 | 15 | 25 | 24 | 17 | 13 | 23 | 19 | 16 | 3 | 4 | 28 | 11 | 7 | 6 | 16 | 46 |
Unique Cell Count | 290 | 140 | 106 | 156 | 228 | 281 | 361 | 415 | 295 | 397 | 315 | 422 | 485 | 752 | 833 | 282 | 120 | 127 | 347 | 157 | 123 | 346 | 534 | 628 |
Labelled Cell Count | 313 | 148 | 121 | 180 | 255 | 358 | 459 | 514 | 381 | 499 | 413 | 556 | 497 | 770 | 851 | 326 | 129 | 141 | 347 | 157 | 123 | 346 | 534 | 628 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.8 | 4.4 | 4.5 | 4.4 | 3.7 | 3.3 | 3.5 | 3.3 | 3.1 | 3.1 | 3.0 | 5.9 | 5.8 | 5.8 | 5.0 | 5.9 | 6.3 | 5.7 | 5.6 | 6.2 |
Std Deviation (1e-4) | 0.7 | 1.0 | 0.7 | 1.1 | 1.1 | 1.1 | 1.1 | 0.7 | 0.9 | 1.0 | 1.0 | 1.2 | 1.0 | 1.2 | 1.3 | 1.2 | 1.5 | 1.2 | 1.8 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.0 | -0.23 | -0.41 | -0.34 | -0.39 | -0.48 | -0.48 | -0.55 | 0.44 | 0.42 | 0.41 | 0.2 | 0.43 | 0.52 | 0.38 | 0.35 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | -4.5 | -7.6 | -8.0 | -8.7 | -8.5 | -9.9 | -10.1 | -8.5 | 2.5 | 4.0 | 4.5 | 0.6 | 1.8 | 0.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.5 | -3.6 | -2.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.7 | 2.0 | 1.8 | 1.9 | 1.4 | 0.5 | 1.0 | 1.4 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 |