Standard name
Human Ortholog
Description S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated UPR; regulates HAC1 mRNA translocation, splicing and translation with KIN1 during ER stress; direct phosphorylation of Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the PM and sites of polarized growth; may regulate septin and cell wall organization; activation loop phosphorylation (T281) required for full kinase activity; orthologous to MARK/PAR-1, AMPK family members
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.1 0.05 0.05 0.07 0.12 0.09 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0.06 0.1 0.16 0.06 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.11 0 0 0 0 0 0 0 – 0 0 0 0.11 0.06 0.05 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.9 0.9 0.9 0.97 0.91 0.9 0.83 0.77 – 0.72 0.7 0.65 0.97 0.99 0.97 0.92 0.92 0.82 0.71 0.76 0.64 0.36 0.65 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.1 0.22 0.28 0.37 – 0.45 0.46 0.49 0 0 0 0.08 0 0 0 0 0.05 0.07 0.05 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 15 2 0 1 0 0 0 – 0 1 1 3 2 4 12 19 36 27 13 24 27 32 13
Bud 4 8 12 5 4 11 13 21 – 24 18 22 1 2 3 4 5 15 13 6 8 35 17 9
Bud Neck 0 0 1 0 0 0 0 1 – 0 0 0 3 2 0 4 8 0 45 16 36 37 21 12
Bud Site 0 1 1 0 0 1 0 1 – 2 1 1 0 0 0 2 2 3 – – – – – –
Cell Periphery 45 10 15 14 10 12 15 4 – 10 21 17 18 10 15 14 9 23 7 3 10 5 6 6
Cytoplasm 381 237 324 323 320 463 555 591 – 452 363 427 155 159 286 512 389 301 373 213 242 82 241 297
Endoplasmic Reticulum 0 7 0 1 3 2 6 0 – 2 3 4 5 2 2 30 17 22 20 2 13 7 8 12
Endosome 21 0 5 0 5 4 4 11 – 9 5 6 0 0 0 1 0 2 9 6 8 3 4 4
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 4 1 1 1 2 2
Mitochondria 11 8 12 20 36 116 190 279 – 287 237 320 0 0 0 43 19 10 9 6 17 16 18 10
Nucleus 1 1 0 1 0 0 2 4 – 5 2 4 0 0 0 2 5 1 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 4 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 2 0 1 1 0 0 3 1 – 2 2 0 0 0 0 0 0 0 1 0 0 0 1 0
Peroxisomes 4 1 9 2 4 0 2 1 – 0 0 0 0 0 0 0 0 5 0 0 0 0 0 0
SpindlePole 13 0 7 1 3 1 5 3 – 3 3 0 0 0 0 8 9 14 2 0 1 1 1 1
Vac/Vac Membrane 4 0 1 0 8 3 7 15 – 5 9 15 0 0 1 1 0 5 7 5 5 5 7 14
Unique Cell Count 422 264 359 334 352 517 668 763 631 517 653 159 161 294 554 425 367 529 280 379 228 370 392
Labelled Cell Count 489 288 390 368 394 613 802 936 801 665 818 185 177 311 633 482 437 529 280 379 228 370 392


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.9 4.8 5.0 4.6 4.0 4.1 4.1 – 3.7 3.7 3.8 6.6 6.6 7.0 4.9 5.1 6.9 6.9 6.9 7.0
Std Deviation (1e-4) 0.6 0.8 0.8 1.2 1.2 1.0 1.2 0.8 – 1.2 0.9 0.9 0.9 0.9 1.0 1.0 0.8 1.2 1.5 1.4 1.6
Intensity Change (Log2) – – – 0.06 -0.04 -0.24 -0.24 -0.24 – -0.37 -0.38 -0.32 0.46 0.46 0.54 0.04 0.09 0.52 0.53 0.53 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 1.9 3.4 5.6
Bud -1.6 0 0 0 0 – 0 0 0 0 0 -2.0 -2.9 -2.1 0.5
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 3.1 1.0 0.6 -1.4 -1.7 1.3
Cytoplasm 3.4 0.3 -0.3 -3.1 -5.2 – -6.8 -7.1 -8.6 2.9 3.5 3.6 1.2 0.6 -3.2
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 4.5 3.8 4.7
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 1.7 0 0 0 0 – 0 0 0 0 0 -3.2 2.7 0.8 -0.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes -2.0 0 0 0 0 – 0 0 0 0 0 0 0 0 -1.1
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 -0.6 0.2 1.5
Vacuole 0 0 0 0 2.2 – 0 0 2.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.031 0.0024 0.0294 0.0319 0.0726 0.0183 0.0582 0.0041 0.032 0.0489 0.023 0.0184 – – – – – –
Bud 0.0033 0.0065 0.0181 0.0122 0.0018 0.0032 0.0029 0.0025 0.0215 0.0105 0.0061 0.0008 – – – – – –
Bud Neck 0.009 0.0012 0.0052 0.0028 0.0246 0.1718 0.0092 0.0018 0.0009 0.0081 0.0014 0.22 – – – – – –
Bud Periphery 0.0078 0.0083 0.0535 0.0464 0.0038 0.0046 0.0076 0.0016 0.0538 0.0422 0.0267 0.0021 – – – – – –
Bud Site 0.0265 0.0538 0.029 0.0033 0.0014 0.004 0.0305 0.1016 0.0039 0.0147 0.0027 0.0027 – – – – – –
Cell Periphery 0.0063 0.0044 0.004 0.0033 0.0011 0.0023 0.0121 0.0028 0.0039 0.0058 0.0061 0.002 – – – – – –
Cytoplasm 0.5877 0.7837 0.6742 0.7756 0.6778 0.6564 0.611 0.8044 0.6671 0.6378 0.6784 0.6388 – – – – – –
Cytoplasmic Foci 0.0354 0.0115 0.0172 0.0129 0.0309 0.0157 0.0375 0.0127 0.0185 0.0203 0.0296 0.0056 – – – – – –
Eisosomes 0.0004 0.0001 0.0003 0.0002 0.0005 0.0002 0.0008 0.0001 0.0005 0.0009 0.0003 0.0001 – – – – – –
Endoplasmic Reticulum 0.0146 0.0044 0.0053 0.0075 0.0115 0.0056 0.0096 0.0043 0.0059 0.003 0.0045 0.0019 – – – – – –
Endosome 0.0298 0.006 0.0163 0.0246 0.0463 0.0101 0.018 0.0041 0.015 0.0226 0.0047 0.0065 – – – – – –
Golgi 0.0058 0.0013 0.0054 0.0053 0.0147 0.0025 0.0148 0.0015 0.0077 0.0095 0.0044 0.0032 – – – – – –
Lipid Particles 0.0052 0.0006 0.002 0.0014 0.0204 0.0012 0.0123 0.0017 0.007 0.0127 0.0122 0.001 – – – – – –
Mitochondria 0.0075 0.0004 0.0039 0.0033 0.0029 0.0011 0.0036 0.0001 0.0095 0.0034 0.0031 0.0118 – – – – – –
None 0.167 0.1074 0.1165 0.0544 0.0381 0.0874 0.1364 0.0514 0.1321 0.1431 0.1217 0.0808 – – – – – –
Nuclear Periphery 0.0225 0.0006 0.0072 0.0019 0.006 0.0065 0.0017 0.0005 0.002 0.0011 0.0019 0.0012 – – – – – –
Nucleolus 0.0011 0.0003 0.0003 0.0002 0.0009 0.0003 0.0004 0.0001 0.0002 0.0006 0.004 0.0001 – – – – – –
Nucleus 0.0142 0.0019 0.0023 0.0017 0.0241 0.0029 0.001 0.0008 0.001 0.0013 0.0025 0.0006 – – – – – –
Peroxisomes 0.0045 0.0002 0.0021 0.0021 0.0074 0.0007 0.0221 0.0002 0.0127 0.0056 0.054 0.0007 – – – – – –
Punctate Nuclear 0.0119 0.0006 0.0022 0.0012 0.0075 0.002 0.0061 0.0008 0.0017 0.0035 0.0103 0.0005 – – – – – –
Vacuole 0.0066 0.0039 0.0046 0.0067 0.0047 0.0025 0.0035 0.0029 0.0026 0.0038 0.0018 0.001 – – – – – –
Vacuole Periphery 0.0021 0.0002 0.001 0.0011 0.001 0.0005 0.0005 0.0002 0.0006 0.0006 0.0005 0.0003 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.5823 16.6114 15.9393 26.9272 22.8466 18.572 23.0942 24.2357 24.5365 27.6109
Translational Efficiency 0.8695 0.7716 0.8429 0.4577 0.5582 0.8693 0.6385 0.5414 0.5531 0.5602

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1039 1272 942 1339 1584 1792 2395 1679 2623 3064 3337 3018

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 833.94 823.07 852.79 977.99 623.02 757.93 916.88 929.05 706.57 784.97 898.79 950.76
Standard Deviation 96.02 104.75 95.95 124.80 70.84 106.97 107.57 119.80 131.62 110.80 108.33 124.44
Intensity Change Log 2 — -0.018928 0.032247 0.229876 — 0.282786 0.557454 0.576478 — 0.117876 0.280519 0.388374

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002405 0.001425 0.001676 0.016320 0.001464 0.001044 0.001882 0.003720 0.001837 0.001202 0.001824 0.009310
Bud Neck 0.031917 0.055042 0.013258 0.019725 0.095789 0.062666 0.035116 0.024881 0.070489 0.059501 0.028946 0.022594
Bud Site 0.079818 0.076939 0.079074 0.136295* 0.069525 0.064429 0.089136 0.123424* 0.073602 0.069622 0.086295 0.129134*
Cell Periphery 0.000378 0.000435 0.000364 0.000965 0.001966 0.000243 0.000234 0.000432 0.001337 0.000323 0.000271 0.000668
Cytoplasm 0.471354* 0.507385* 0.618748* 0.489726* 0.487583* 0.485127* 0.668674* 0.614056* 0.481154* 0.494367* 0.654581* 0.558895*
Cytoplasmic Foci 0.057510 0.192196* 0.083562 0.046174 0.196721* 0.192927* 0.037983 0.055883 0.141578 0.192624* 0.050849 0.051575
Eisosomes 0.000221 0.000574 0.000088 0.000533 0.002847 0.000404 0.000106 0.000263 0.001807 0.000475 0.000101 0.000383
Endoplasmic Reticulum 0.000486 0.000910 0.002040 0.001598 0.002548 0.000584 0.000331 0.000533 0.001731 0.000720 0.000814 0.001005
Endosome 0.010571 0.021928 0.007109 0.004839 0.011162 0.029401 0.002960 0.003401 0.010928 0.026298 0.004131 0.004039
Golgi 0.002162 0.009157 0.000794 0.002353 0.020404 0.008999 0.000426 0.001516 0.013178 0.009064 0.000530 0.001887
Lipid Particles 0.002619 0.003350 0.001687 0.001640 0.010334 0.004480 0.000457 0.000556 0.007278 0.004011 0.000804 0.001037
Mitochondria 0.001876 0.001970 0.000117 0.002427 0.024251 0.005927 0.000243 0.000909 0.015388 0.004284 0.000207 0.001582
Mitotic Spindle 0.008050 0.001297 0.001457 0.012540 0.000154 0.004113 0.003040 0.001695 0.003282 0.002944 0.002593 0.006506
None 0.000305 0.000652 0.001444 0.000305 0.004907 0.001033 0.000431 0.001014 0.003084 0.000875 0.000717 0.000699
Nuclear Periphery 0.000132 0.000058 0.000168 0.000116 0.000303 0.000115 0.000053 0.000043 0.000235 0.000092 0.000086 0.000076
Nuclear Periphery Foci 0.001226 0.000273 0.004702 0.001401 0.000379 0.000192 0.000653 0.000675 0.000714 0.000225 0.001796 0.000997
Nucleolus 0.000174 0.000265 0.000170 0.000127 0.000438 0.000379 0.000077 0.000059 0.000333 0.000332 0.000103 0.000089
Nucleus 0.025866 0.022279 0.066773 0.023707 0.029763 0.019797 0.028172 0.018465 0.028219 0.020827 0.039068 0.020791
Peroxisomes 0.003978 0.006323 0.003166 0.005690 0.006687 0.009203 0.003021 0.003411 0.005614 0.008007 0.003062 0.004422
Vacuole 0.298371* 0.095655 0.113346 0.232161* 0.029683 0.106748 0.126853 0.144837 0.136113 0.102143 0.123040 0.183580
Vacuole Periphery 0.000581 0.001887 0.000259 0.001358 0.003092 0.002190 0.000152 0.000226 0.002097 0.002064 0.000182 0.000729

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.69 0.38 -5.18 -6.47 -6.24 2.20 -0.97 -5.13 -7.17 -4.48 1.31 0.01 -6.39 -7.96 -7.15
Bud Neck -6.02 4.47 4.24 11.36 -0.68 6.16 13.96 15.93 11.35 3.26 2.48 13.41 16.13 15.96 3.75
Bud Site -1.14 -0.24 -3.71 -2.89 -3.28 1.36 -1.03 -5.74 -6.79 -4.79 0.13 -0.99 -6.75 -7.06 -5.75
Cell Periphery -2.05 -0.42 -6.09 -3.90 -5.67 14.47 14.80 12.91 -2.99 -3.91 13.12 14.37 10.04 -5.10 -7.73
Cytoplasm -4.26 -11.42 0.99 5.65 13.06 0.29 -20.06 -12.01 -11.76 6.32 -2.62 -23.28 -7.28 -4.86 15.10
Cytoplasmic Foci -25.25 -6.30 4.75 28.63 10.16 0.58 36.29 30.62 28.47 -7.16 -11.69 27.33 27.75 40.19 0.54
Eisosomes -5.89 7.44 -5.41 2.34 -8.75 13.80 15.81 14.70 3.33 -3.04 11.75 15.74 13.39 3.93 -7.41
Endoplasmic Reticulum -1.50 -8.82 -4.82 -1.75 2.68 6.15 7.01 6.02 0.15 -1.43 4.67 4.43 3.66 -1.52 -0.65
Endosome -8.86 3.57 8.27 13.88 4.63 -12.42 16.75 14.39 18.52 -0.86 -15.19 15.80 16.16 23.04 1.86
Golgi -7.65 1.70 0.63 9.49 -1.51 10.28 22.89 20.06 9.74 -2.08 5.10 20.54 17.54 13.48 -2.78
Lipid Particles -1.13 0.66 0.70 3.88 0.14 7.14 14.80 14.64 7.95 -1.61 5.08 11.89 11.39 8.69 -2.52
Mitochondria -0.43 1.51 0.16 1.35 -3.24 10.19 14.67 14.04 6.21 -1.33 9.40 13.95 12.82 6.24 -3.05
Mitotic Spindle 2.90 2.95 -0.91 -4.00 -4.08 -3.63 -3.21 -3.27 2.04 0.95 -0.11 0.69 -1.97 -1.89 -2.77
None -2.26 -3.75 -0.19 2.51 3.83 4.99 6.72 5.30 0.31 -1.16 4.70 5.80 5.38 0.78 0.27
Nuclear Periphery 4.20 -1.80 -0.72 -6.23 1.24 0.82 0.98 1.06 1.75 3.19 1.10 0.95 0.97 -1.03 0.25
Nuclear Periphery Foci 1.61 -5.24 -0.23 -8.84 8.23 0.86 -1.55 -1.79 -6.28 -0.56 1.82 -4.12 -1.20 -10.70 5.79
Nucleolus -2.05 0.03 1.34 4.40 2.06 0.59 6.09 6.41 5.20 3.55 -0.11 5.74 6.13 6.45 1.89
Nucleus 2.91 -12.21 -0.02 -3.79 13.25 5.97 -3.78 4.97 -1.29 9.08 6.38 -10.14 3.39 -3.65 14.24
Peroxisomes -4.62 0.93 -0.69 2.76 -1.47 -3.50 8.86 7.15 9.05 -1.51 -5.27 7.29 3.82 8.42 -2.18
Vacuole 26.10* 19.51 -0.71 -28.31 -21.11 -23.20 -32.92 -30.71 -13.90 -4.96 9.06 -0.97 -16.77 -29.30 -18.24
Vacuole Periphery -4.47 1.40 -0.86 3.18 -2.09 3.68 16.27 16.04 11.23 -1.29 0.07 14.16 9.40 8.78 -2.45
Standard name
Human Ortholog
Description S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated UPR; regulates HAC1 mRNA translocation, splicing and translation with KIN1 during ER stress; direct phosphorylation of Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the PM and sites of polarized growth; may regulate septin and cell wall organization; activation loop phosphorylation (T281) required for full kinase activity; orthologous to MARK/PAR-1, AMPK family members
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Kin2

Kin2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kin2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available