Standard name
Description S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated UPR; regulates HAC1 mRNA translocation, splicing and translation with KIN1 during ER stress; direct phosphorylation of Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the PM and sites of polarized growth; may regulate septin and cell wall organization; activation loop phosphorylation (T281) required for full kinase activity; orthologous to MARK/PAR-1, AMPK family members

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0.05 0.07 0.12 0.09 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.1 0.16 0.06 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.11 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0.05 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.9 0.9 0.9 0.97 0.91 0.9 0.83 0.77 0.72 0.7 0.65 0.97 0.99 0.97 0.92 0.92 0.82 0.71 0.76 0.64 0.36 0.65 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.1 0.22 0.28 0.37 0.45 0.46 0.49 0 0 0 0.08 0 0 0 0 0.05 0.07 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 15 2 0 1 0 0 0 0 1 1 3 2 4 12 19 36 27 13 24 27 32 13
Bud 4 8 12 5 4 11 13 21 24 18 22 1 2 3 4 5 15 13 6 8 35 17 9
Bud Neck 0 0 1 0 0 0 0 1 0 0 0 3 2 0 4 8 0 45 16 36 37 21 12
Bud Site 0 1 1 0 0 1 0 1 2 1 1 0 0 0 2 2 3
Cell Periphery 45 10 15 14 10 12 15 4 10 21 17 18 10 15 14 9 23 7 3 10 5 6 6
Cytoplasm 381 237 324 323 320 463 555 591 452 363 427 155 159 286 512 389 301 373 213 242 82 241 297
Endoplasmic Reticulum 0 7 0 1 3 2 6 0 2 3 4 5 2 2 30 17 22 20 2 13 7 8 12
Endosome 21 0 5 0 5 4 4 11 9 5 6 0 0 0 1 0 2 9 6 8 3 4 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 1 1 2 2
Mitochondria 11 8 12 20 36 116 190 279 287 237 320 0 0 0 43 19 10 9 6 17 16 18 10
Nucleus 1 1 0 1 0 0 2 4 5 2 4 0 0 0 2 5 1 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 4 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 2 0 1 1 0 0 3 1 2 2 0 0 0 0 0 0 0 1 0 0 0 1 0
Peroxisomes 4 1 9 2 4 0 2 1 0 0 0 0 0 0 0 0 5 0 0 0 0 0 0
SpindlePole 13 0 7 1 3 1 5 3 3 3 0 0 0 0 8 9 14 2 0 1 1 1 1
Vac/Vac Membrane 4 0 1 0 8 3 7 15 5 9 15 0 0 1 1 0 5 7 5 5 5 7 14
Unique Cell Count 422 264 359 334 352 517 668 763 631 517 653 159 161 294 554 425 367 529 280 379 228 370 392
Labelled Cell Count 489 288 390 368 394 613 802 936 801 665 818 185 177 311 633 482 437 529 280 379 228 370 392


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.9 4.8 5.0 4.6 4.0 4.1 4.1 3.7 3.7 3.8 6.6 6.6 7.0 4.9 5.1 6.9 6.9 6.9 7.0
Std Deviation (1e-4) 0.6 0.8 0.8 1.2 1.2 1.0 1.2 0.8 1.2 0.9 0.9 0.9 0.9 1.0 1.0 0.8 1.2 1.5 1.4 1.6
Intensity Change (Log2) 0.06 -0.04 -0.24 -0.24 -0.24 -0.37 -0.38 -0.32 0.46 0.46 0.54 0.04 0.09 0.52 0.53 0.53 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1.9 3.4 5.6
Bud -1.6 0 0 0 0 0 0 0 0 0 -2.0 -2.9 -2.1 0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 3.1 1.0 0.6 -1.4 -1.7 1.3
Cytoplasm 3.4 0.3 -0.3 -3.1 -5.2 -6.8 -7.1 -8.6 2.9 3.5 3.6 1.2 0.6 -3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 4.5 3.8 4.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.7 0 0 0 0 0 0 0 0 0 -3.2 2.7 0.8 -0.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -2.0 0 0 0 0 0 0 0 0 0 0 0 0 -1.1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 -0.6 0.2 1.5
Vacuole 0 0 0 0 2.2 0 0 2.5 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kin2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available