Standard name | |
---|---|
Human Ortholog | |
Description | Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII during osmotic shock; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; binds calmodulin; stimulates antisense transcription to activate CDC28; defines novel S-phase checkpoint with Mrc1p that prevent replication/transcription conflicts; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 |
Bud | 1 | 3 | 4 | 1 | 3 | 4 | 25 | 12 | 11 | 16 | 18 | 13 | 1 | 2 | 0 | 3 | 3 | 0 | 0 | 1 | 1 | 8 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 9 | 16 | 147 | 130 | 148 | 212 | 176 | 183 | 220 | 178 | 182 | 149 | 110 | 120 | 92 | 43 | 47 | 52 | 0 | 1 | 3 | 0 | 0 | 2 |
Cytoplasm | 98 | 348 | 787 | 510 | 600 | 730 | 722 | 797 | 584 | 662 | 505 | 529 | 533 | 469 | 403 | 429 | 375 | 398 | 96 | 325 | 769 | 551 | 508 | 519 |
Endoplasmic Reticulum | 0 | 1 | 13 | 7 | 11 | 17 | 19 | 9 | 35 | 40 | 14 | 21 | 5 | 4 | 13 | 7 | 8 | 5 | 1 | 1 | 12 | 2 | 2 | 6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 3 | 1 | 3 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 3 | 0 | 0 | 2 |
Mitochondria | 1 | 0 | 1 | 0 | 5 | 6 | 22 | 12 | 15 | 28 | 19 | 22 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 1 |
Nucleus | 0 | 2 | 6 | 2 | 2 | 1 | 3 | 2 | 1 | 0 | 1 | 1 | 2 | 8 | 45 | 24 | 7 | 3 | 0 | 1 | 0 | 4 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 8 | 8 | 5 | 14 | 18 | 3 | 4 | 5 | 3 | 1 | 2 | 13 | 3 | 2 | 10 | 0 | 0 | 2 | 4 | 1 | 2 |
Unique Cell Count | 99 | 350 | 804 | 535 | 641 | 780 | 798 | 858 | 661 | 762 | 580 | 602 | 538 | 476 | 438 | 446 | 385 | 415 | 106 | 341 | 808 | 586 | 526 | 552 |
Labelled Cell Count | 109 | 370 | 962 | 658 | 778 | 977 | 983 | 1036 | 871 | 934 | 748 | 740 | 652 | 606 | 566 | 514 | 445 | 471 | 106 | 341 | 808 | 586 | 526 | 552 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.1 | 17.4 | 14.0 | 14.4 | 14.8 | 14.7 | 13.5 | 14.2 | 14.1 | 13.1 | 12.8 | 12.1 | 17.6 | 17.5 | 16.9 | 17.4 | 15.5 | 17.6 | 18.2 | 18.1 | 18.7 |
Std Deviation (1e-4) | 2.5 | 3.5 | 2.2 | 2.2 | 2.4 | 2.3 | 2.4 | 2.5 | 2.5 | 2.4 | 2.5 | 2.6 | 3.4 | 3.7 | 3.7 | 4.4 | 4.2 | 4.2 | 3.3 | 3.5 | 3.4 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.08 | 0.08 | -0.04 | 0.03 | 0.01 | -0.09 | -0.13 | -0.21 | 0.33 | 0.32 | 0.28 | 0.31 | 0.15 | 0.33 | 0.38 | 0.38 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 4.0 | 1.9 | 2.2 | 2.8 | 3.8 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.7 | 2.3 | 4.2 | 1.9 | 1.6 | 6.6 | 2.5 | 5.6 | 2.9 | 1.0 | 2.9 | 1.2 | -4.1 | -2.7 | -2.6 |
Cytoplasm | -2.6 | -4.1 | -4.2 | -6.3 | -4.8 | -7.4 | -8.3 | -8.0 | -7.6 | 1.7 | 0.8 | -4.9 | -1.8 | -0.5 | -2.0 |
Endoplasmic Reticulum | -0.5 | 0.1 | 0.8 | 1.1 | -1.0 | 3.9 | 4.0 | 1.1 | 2.3 | -1.1 | -1.2 | 1.6 | -0.1 | 0.6 | -0.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.4 | 2.9 | 3.9 | 5.2 | 4.8 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 8.1 | 5.1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 19.0239 | 21.1992 | 20.3625 | 18.864 | 17.9725 | 20.5494 | 20.9866 | 22.3812 | 21.8153 | 20.8444 | 20.7008 | 20.7923 | 19.4411 | 22.4106 | 21.4145 | 20.5784 | 20.7075 | 21.1955 |
Actin | 0.0134 | 0.0026 | 0.0136 | 0.0303 | 0.026 | 0.0044 | 0.0005 | 0.0007 | 0.0047 | 0.0008 | 0.0027 | 0.0005 | 0.0071 | 0.0088 | 0.0065 | 0.0089 | 0.0082 | 0.0089 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0.0014 | 0.0009 | 0.0005 | 0.0003 | 0.0002 | 0.0025 | 0 | 0.0054 | 0.0008 | 0.0057 | 0.013 | 0.0168 | 0.0043 | 0.0056 | 0.0063 |
Bud Neck | 0.0023 | 0.0004 | 0.0012 | 0.0063 | 0.0029 | 0.0039 | 0.0013 | 0.0008 | 0.0013 | 0.0014 | 0.0031 | 0.0044 | 0.001 | 0.0008 | 0.0008 | 0.0013 | 0.0043 | 0.007 |
Bud Periphery | 0.0004 | 0 | 0.0002 | 0.0016 | 0.0013 | 0.0018 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0085 | 0.0003 | 0.0028 | 0.0025 | 0.0069 | 0.0015 | 0.0023 | 0.0052 |
Bud Site | 0.0021 | 0.0017 | 0.0043 | 0.0068 | 0.0009 | 0.0006 | 0.0023 | 0.0017 | 0.0024 | 0.0001 | 0.0032 | 0.0002 | 0.0079 | 0.0155 | 0.0131 | 0.0006 | 0.0013 | 0.0006 |
Cell Periphery | 0.0014 | 0.0003 | 0.0006 | 0.0006 | 0.0008 | 0.0007 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0018 | 0.0002 | 0.0012 | 0.001 | 0.0017 | 0.0003 | 0.0012 | 0.0008 |
Cytoplasm | 0.9279 | 0.9899 | 0.9495 | 0.865 | 0.9029 | 0.972 | 0.9863 | 0.9772 | 0.9708 | 0.9929 | 0.9671 | 0.9824 | 0.933 | 0.9445 | 0.9241 | 0.9608 | 0.9646 | 0.9603 |
Cytoplasmic Foci | 0.0134 | 0.001 | 0.0057 | 0.0133 | 0.0082 | 0.0018 | 0.0011 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0.0007 | 0.0063 | 0.0012 | 0.0008 | 0.0022 | 0.0022 | 0.0009 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0004 | 0.001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0048 | 0.0012 | 0.0016 | 0.0029 | 0.0122 | 0.0024 | 0.0014 | 0.0015 | 0.0013 | 0.001 | 0.002 | 0.0018 | 0.0044 | 0.0013 | 0.0022 | 0.0017 | 0.0021 | 0.0013 |
Endosome | 0.0072 | 0.0002 | 0.0014 | 0.0111 | 0.0115 | 0.0023 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0015 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Golgi | 0.0023 | 0.0001 | 0.0019 | 0.0039 | 0.0087 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Lipid Particles | 0.0057 | 0.0001 | 0.0022 | 0.0068 | 0.0028 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0036 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0001 |
Mitochondria | 0.0011 | 0 | 0.0007 | 0.0239 | 0.0068 | 0.0011 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0051 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0001 |
None | 0.0052 | 0.0006 | 0.0022 | 0.0038 | 0.0017 | 0.0013 | 0.0017 | 0.0013 | 0.0011 | 0.0006 | 0.0006 | 0.0018 | 0.0031 | 0.0017 | 0.0016 | 0.0062 | 0.002 | 0.0013 |
Nuclear Periphery | 0.0013 | 0.0003 | 0.0005 | 0.0032 | 0.0055 | 0.001 | 0.0004 | 0.0007 | 0.0012 | 0.0003 | 0.0005 | 0.0005 | 0.0038 | 0.0015 | 0.001 | 0.0026 | 0.0008 | 0.0011 |
Nucleolus | 0.0003 | 0 | 0.0003 | 0.0015 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0035 | 0.0012 | 0.0079 | 0.0045 | 0.002 | 0.0027 | 0.0023 | 0.0137 | 0.0106 | 0.0018 | 0.0031 | 0.0055 | 0.0052 | 0.0057 | 0.0215 | 0.0045 | 0.002 | 0.0044 |
Peroxisomes | 0.0036 | 0 | 0.003 | 0.0057 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0023 | 0.0001 | 0.0026 | 0.0026 | 0.0009 | 0.0005 | 0.0003 | 0.0009 | 0.0015 | 0.0001 | 0.0003 | 0.0006 | 0.0039 | 0.0011 | 0.0006 | 0.0035 | 0.001 | 0.0012 |
Vacuole | 0.001 | 0.0001 | 0.0003 | 0.0028 | 0.0013 | 0.0014 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.001 | 0.0004 | 0.0009 | 0.0004 | 0.0005 | 0.0003 |
Vacuole Periphery | 0.0001 | 0 | 0 | 0.0013 | 0.0009 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 71.2826 | 77.6734 | 55.3987 | 80.2977 | 80.9368 | 68.2923 | 83.6822 | 80.2811 | 76.2906 | 99.0266 |
Translational Efficiency | 0.9619 | 0.8573 | 0.8621 | 0.5483 | 0.6772 | 1.1078 | 0.7869 | 0.7566 | 0.8361 | 0.6682 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1748 | 909 | 2916 | 1562 | 1820 | 1749 | 117 | 78 | 3568 | 2658 | 3033 | 1640 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1247.02 | 1277.66 | 1723.30 | 1692.52 | 1228.25 | 1352.88 | 1844.87 | 2051.13 | 1237.45 | 1327.16 | 1727.99 | 1709.58 |
Standard Deviation | 175.10 | 296.11 | 242.48 | 301.09 | 170.71 | 214.19 | 249.59 | 375.77 | 173.13 | 247.89 | 243.89 | 314.46 |
Intensity Change Log 2 | — | 0.035019 | 0.466689 | 0.440688 | — | 0.139430 | 0.586915 | 0.739815 | — | 0.087773 | 0.527598 | 0.596860 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001891 | 0.000810 | 0.002686 | 0.004450 | 0.001644 | 0.001894 | 0.004556 | 0.006266 | 0.001765 | 0.001523 | 0.002758 | 0.004536 |
Bud Neck | 0.003115 | 0.002430 | 0.003929 | 0.005225 | 0.003789 | 0.002483 | 0.007280 | 0.011810 | 0.003459 | 0.002465 | 0.004058 | 0.005538 |
Bud Site | 0.033426 | 0.022903 | 0.027630 | 0.039017 | 0.030620 | 0.026714 | 0.042477 | 0.081053 | 0.031995 | 0.025411 | 0.028203 | 0.041017 |
Cell Periphery | 0.001946 | 0.000789 | 0.001304 | 0.001607 | 0.002116 | 0.000650 | 0.001266 | 0.005610 | 0.002033 | 0.000698 | 0.001302 | 0.001797 |
Cytoplasm | 0.835840* | 0.840461* | 0.833959* | 0.712868* | 0.797968* | 0.800056* | 0.336702* | 0.436471* | 0.816522* | 0.813874* | 0.814777* | 0.699722* |
Cytoplasmic Foci | 0.047574 | 0.046162 | 0.018902 | 0.025486 | 0.060906 | 0.066344 | 0.061838 | 0.103133 | 0.054374 | 0.059442 | 0.020559 | 0.029179 |
Eisosomes | 0.000131 | 0.000023 | 0.000069 | 0.000040 | 0.000174 | 0.000047 | 0.000101 | 0.000129 | 0.000153 | 0.000039 | 0.000070 | 0.000044 |
Endoplasmic Reticulum | 0.001122 | 0.000403 | 0.000117 | 0.001030 | 0.001787 | 0.000732 | 0.001586 | 0.018299 | 0.001461 | 0.000619 | 0.000174 | 0.001851 |
Endosome | 0.000793 | 0.001555 | 0.000306 | 0.009711 | 0.000997 | 0.001754 | 0.008234 | 0.002687 | 0.000897 | 0.001686 | 0.000612 | 0.009377 |
Golgi | 0.001789 | 0.002024 | 0.000245 | 0.007689 | 0.001725 | 0.002339 | 0.000842 | 0.000671 | 0.001756 | 0.002231 | 0.000268 | 0.007355 |
Lipid Particles | 0.002742 | 0.002175 | 0.003720 | 0.007702 | 0.002844 | 0.002866 | 0.012841 | 0.037847 | 0.002794 | 0.002630 | 0.004071 | 0.009136 |
Mitochondria | 0.000290 | 0.000358 | 0.000090 | 0.002195 | 0.000159 | 0.000415 | 0.052550 | 0.000013 | 0.000223 | 0.000396 | 0.002114 | 0.002091 |
Mitotic Spindle | 0.000809 | 0.001173 | 0.000651 | 0.017798 | 0.001091 | 0.004839 | 0.001234 | 0.000795 | 0.000953 | 0.003585 | 0.000674 | 0.016990 |
None | 0.005241 | 0.009762 | 0.003306 | 0.000995 | 0.008226 | 0.006403 | 0.000313 | 0.000918 | 0.006764 | 0.007552 | 0.003190 | 0.000992 |
Nuclear Periphery | 0.000037 | 0.000044 | 0.000046 | 0.000242 | 0.000046 | 0.000051 | 0.000260 | 0.000282 | 0.000041 | 0.000049 | 0.000054 | 0.000244 |
Nuclear Periphery Foci | 0.004851 | 0.010765 | 0.002530 | 0.004325 | 0.008617 | 0.007416 | 0.024341 | 0.023975 | 0.006772 | 0.008561 | 0.003371 | 0.005260 |
Nucleolus | 0.000203 | 0.000958 | 0.000105 | 0.000403 | 0.000210 | 0.000340 | 0.000045 | 0.000027 | 0.000206 | 0.000551 | 0.000103 | 0.000385 |
Nucleus | 0.029136 | 0.031920 | 0.046166 | 0.025265 | 0.044427 | 0.037563 | 0.023199 | 0.016265 | 0.036936 | 0.035633 | 0.045280 | 0.024837 |
Peroxisomes | 0.000608 | 0.000625 | 0.000893 | 0.003380 | 0.000586 | 0.001009 | 0.002173 | 0.000456 | 0.000597 | 0.000878 | 0.000943 | 0.003241 |
Vacuole | 0.027847 | 0.023971 | 0.053285 | 0.125047 | 0.031848 | 0.035701 | 0.417895* | 0.252887* | 0.029888 | 0.031689 | 0.067350 | 0.131127 |
Vacuole Periphery | 0.000610 | 0.000689 | 0.000062 | 0.005525 | 0.000221 | 0.000384 | 0.000265 | 0.000405 | 0.000411 | 0.000488 | 0.000070 | 0.005281 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 6.04 | -3.85 | -2.11 | -4.99 | 0.90 | -0.56 | -1.60 | -5.59 | -5.08 | -1.51 | 1.56 | -4.72 | -2.99 | -3.54 | 0.67 |
Bud Neck | 1.38 | -5.00 | -2.24 | -2.32 | 1.73 | 4.24 | -5.23 | -6.25 | -7.36 | -3.05 | 3.75 | -4.26 | -2.39 | -5.09 | 0.79 |
Bud Site | 4.90 | 2.93 | 4.16 | -1.49 | 2.18 | 2.62 | -3.92 | -5.70 | -6.43 | -3.12 | 5.22 | 1.90 | 2.66 | -1.81 | 1.36 |
Cell Periphery | 7.35 | 3.90 | 6.80 | -0.54 | 4.87 | 13.38 | 5.82 | -4.61 | -7.45 | -6.34 | 14.40 | 6.57 | 8.13 | -3.33 | 3.14 |
Cytoplasm | 0.80 | 12.64 | 27.36 | 22.17 | 19.32 | 1.40 | 20.44 | 16.05 | 15.66 | -1.21 | 2.97 | 12.69 | 27.64 | 24.09 | 18.38 |
Cytoplasmic Foci | -0.78 | 13.43 | 13.22 | 8.59 | 0.79 | -2.97 | 0.07 | -2.92 | -1.88 | -2.63 | -3.92 | 20.34 | 17.58 | 16.70 | -0.50 |
Eisosomes | 10.34 | 5.59 | 9.63 | -2.96 | 12.57 | 13.48 | 8.50 | 3.35 | -6.18 | -3.02 | 16.11 | 11.16 | 16.71 | 1.28 | 11.46 |
Endoplasmic Reticulum | 2.39 | 3.21 | 2.61 | 0.67 | -2.22 | 2.58 | 1.72 | -0.59 | -0.88 | -0.79 | 3.28 | 4.92 | 2.97 | 0.33 | -1.55 |
Endosome | -2.24 | 6.51 | -3.88 | -1.98 | -4.90 | -2.94 | -5.46 | -0.66 | 0.91 | 4.35 | -3.93 | 5.83 | -3.70 | -2.03 | -4.63 |
Golgi | 0.08 | 7.72 | 0.23 | 0.12 | -2.63 | -0.54 | 6.22 | 6.63 | 3.46 | 0.57 | -0.40 | 10.80 | 0.29 | 0.49 | -2.63 |
Lipid Particles | 1.67 | -6.46 | -7.34 | -7.99 | -1.68 | -0.08 | -3.74 | -6.11 | -6.10 | -4.45 | 0.54 | -7.27 | -9.13 | -8.91 | -3.45 |
Mitochondria | -0.06 | 1.83 | -0.88 | -0.79 | -2.22 | -1.28 | -0.98 | 4.01 | 2.40 | 1.00 | -0.89 | -0.52 | -1.28 | -0.64 | -0.18 |
Mitotic Spindle | -0.54 | 0.63 | -4.13 | -3.96 | -4.44 | -2.31 | -0.63 | 1.03 | 3.17 | 2.59 | -2.50 | 0.97 | -4.02 | -2.34 | -4.41 |
None | -2.38 | 4.83 | 8.28 | 5.81 | 12.32 | 2.09 | 8.70 | 8.52 | 6.35 | -0.43 | 0.05 | 7.82 | 11.19 | 8.33 | 12.30 |
Nuclear Periphery | -1.47 | -5.80 | -7.50 | -6.80 | -6.11 | -1.54 | -9.03 | -4.52 | -4.15 | 3.00 | -2.22 | -10.24 | -7.83 | -7.11 | -5.86 |
Nuclear Periphery Foci | -7.03 | 3.88 | 3.16 | 8.49 | -0.49 | 1.60 | -4.00 | -5.37 | -5.82 | -1.41 | -4.19 | 8.60 | 6.42 | 9.07 | -1.35 |
Nucleolus | -1.41 | 7.15 | 0.83 | 1.49 | -1.64 | -0.97 | 12.72 | 12.38 | 2.57 | 2.20 | -1.69 | 11.34 | 1.13 | 1.92 | -1.61 |
Nucleus | -1.70 | -15.83 | -3.10 | -0.79 | 11.85 | 3.20 | 3.12 | 11.15 | 9.03 | 3.31 | 0.41 | -11.30 | 2.52 | 2.04 | 12.24 |
Peroxisomes | 0.26 | -0.27 | -1.53 | -1.49 | -1.28 | -1.53 | -0.88 | 2.17 | 2.44 | 1.12 | -1.34 | -0.52 | -1.53 | -0.76 | -1.16 |
Vacuole | -1.52 | -27.96 | -37.23 | -34.81 | -25.58 | -6.39 | -19.67 | -12.20 | -11.76 | 2.79 | -6.76 | -28.47 | -38.59 | -36.24 | -23.70 |
Vacuole Periphery | 0.06 | 1.98 | -1.22 | -1.19 | -3.14 | -0.87 | 1.71 | 1.10 | 1.56 | 0.05 | -0.01 | 2.53 | -1.92 | -1.93 | -3.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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