Standard name
Description Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII during osmotic shock; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; binds calmodulin; stimulates antisense transcription to activate CDC28; defines novel S-phase checkpoint with Mrc1p that prevent replication/transcription conflicts; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.09 0.05 0.18 0.24 0.23 0.27 0.22 0.21 0.33 0.23 0.31 0.25 0.2 0.25 0.21 0.1 0.12 0.13 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.95 0.94 0.94 0.9 0.93 0.88 0.87 0.87 0.88 0.99 0.99 0.92 0.96 0.97 0.96 0.91 0.95 0.95 0.94 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 2 2 1 0 0 0 0 0 0 0 0 3 0 0 1
Bud 1 3 4 1 3 4 25 12 11 16 18 13 1 2 0 3 3 0 0 1 1 8 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 9 16 147 130 148 212 176 183 220 178 182 149 110 120 92 43 47 52 0 1 3 0 0 2
Cytoplasm 98 348 787 510 600 730 722 797 584 662 505 529 533 469 403 429 375 398 96 325 769 551 508 519
Endoplasmic Reticulum 0 1 13 7 11 17 19 9 35 40 14 21 5 4 13 7 8 5 1 1 12 2 2 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 1 0 1 1 3 1 3
Golgi 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 2 1 0 0 2 3 0 0 2
Mitochondria 1 0 1 0 5 6 22 12 15 28 19 22 0 0 0 1 2 1 0 0 2 0 1 1
Nucleus 0 2 6 2 2 1 3 2 1 0 1 1 2 8 45 24 7 3 0 1 0 4 1 1
Nuclear Periphery 0 0 1 0 1 1 1 1 1 4 1 1 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 2 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 0 0 2 8 8 5 14 18 3 4 5 3 1 2 13 3 2 10 0 0 2 4 1 2
Unique Cell Count 99 350 804 535 641 780 798 858 661 762 580 602 538 476 438 446 385 415 106 341 808 586 526 552
Labelled Cell Count 109 370 962 658 778 977 983 1036 871 934 748 740 652 606 566 514 445 471 106 341 808 586 526 552


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.1 17.4 14.0 14.4 14.8 14.7 13.5 14.2 14.1 13.1 12.8 12.1 17.6 17.5 16.9 17.4 15.5 17.6 18.2 18.1 18.7
Std Deviation (1e-4) 2.5 3.5 2.2 2.2 2.4 2.3 2.4 2.5 2.5 2.4 2.5 2.6 3.4 3.7 3.7 4.4 4.2 4.2 3.3 3.5 3.4
Intensity Change (Log2) – – – 0.04 0.08 0.08 -0.04 0.03 0.01 -0.09 -0.13 -0.21 0.33 0.32 0.28 0.31 0.15 0.33 0.38 0.38 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 4.0 1.9 2.2 2.8 3.8 2.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.7 2.3 4.2 1.9 1.6 6.6 2.5 5.6 2.9 1.0 2.9 1.2 -4.1 -2.7 -2.6
Cytoplasm -2.6 -4.1 -4.2 -6.3 -4.8 -7.4 -8.3 -8.0 -7.6 1.7 0.8 -4.9 -1.8 -0.5 -2.0
Endoplasmic Reticulum -0.5 0.1 0.8 1.1 -1.0 3.9 4.0 1.1 2.3 -1.1 -1.2 1.6 -0.1 0.6 -0.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.4 2.9 3.9 5.2 4.8 5.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 1.6 8.1 5.1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.0 3.5 0 0 0 0 0 0 4.2 0 0 0