Standard name
Description Aspartic protease; hyperglycosylated member of the yapsin family of proteases, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; involved in nutrient limitation-induced cleavage of the extracellular inhibitory domain of signaling mucin Msb2p, resulting in activation of the filamentous growth MAPK pathway; involved with other yapsins in the cell wall integrity response; role in KEX2-independent processing of the alpha factor precursor

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.1 0 0 0.05 0 0 0 0 0 0.08 0.1 0.06 0.12 0.13 0.17 0 0 0 0 0 0
Cytoplasm 0.87 0.82 0.92 0.9 0.81 0.71 0.67 0.79 0.77 0.63 0.73 0.72 0.68 0.9 0.89 0.9 0.82 0.39 0.4 0.41 0.32 0.09 0.18
Endoplasmic Reticulum 0.08 0.14 0 0.07 0.09 0.16 0.23 0 0.08 0.13 0.12 0.32 0.32 0.07 0 0 0.05 0.34 0.37 0.22 0.34 0.7 0.54
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.05 0.11 0.09 0.24 0.11 0 0 0 0 0 0 0.1 0.06 0.17 0.09 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0.08 0.05 0.05 0.06 0 0 0 0 0.05 0 0 0 0.05 0 0.05 0.05 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 4 5 11 15 20 4 6 6 7 0 0 0 0 3 3 10 11 9 1 2 5
Bud 1 2 0 5 4 5 7 9 9 5 4 0 0 0 0 1 2 5 3 2 1 4 5
Bud Neck 0 0 10 1 0 0 0 0 3 1 2 0 0 0 0 1 1 6 6 10 1 0 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Cell Periphery 4 4 26 8 13 22 22 11 10 9 13 2 15 10 10 12 19 7 3 8 0 2 3
Cytoplasm 285 206 249 274 303 320 372 239 263 210 244 18 102 152 75 83 94 146 116 127 15 6 20
Endoplasmic Reticulum 26 35 9 20 32 70 127 12 29 42 41 8 48 11 3 4 6 128 107 69 16 54 63
Endosome 0 3 1 0 2 2 5 0 3 0 1 0 1 1 1 1 1 2 2 3 1 0 0
Golgi 1 2 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0
Mitochondria 9 3 0 2 3 27 27 34 30 79 37 0 0 0 1 0 1 37 18 53 4 1 4
Nucleus 1 1 0 3 3 4 6 3 3 3 4 2 1 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 1 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
SpindlePole 3 0 0 0 3 0 1 2 0 1 3 0 1 0 0 0 1 2 0 1 0 1 0
Vac/Vac Membrane 5 9 5 6 34 35 27 14 21 6 13 0 0 8 3 2 4 18 12 17 2 2 9
Unique Cell Count 327 252 271 304 373 451 553 301 342 332 334 25 149 169 84 92 115 375 292 310 50 78 118
Labelled Cell Count 337 268 304 324 408 500 616 328 378 363 369 30 169 182 93 107 134 375 292 310 50 78 118


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.7 6.1 6.1 7.1 6.0 6.1 5.4 5.4 4.6 5.1 8.0 7.6 7.0 8.3 8.4 8.1 10.0 10.7
Std Deviation (1e-4) 0.8 1.0 0.9 1.6 1.8 1.2 1.1 1.4 1.0 0.8 1.0 1.9 1.4 1.6 2.0 2.6 2.4 3.0 2.7
Intensity Change (Log2) 0.02 0.23 -0.0 0.01 -0.18 -0.18 -0.39 -0.25 0.39 0.33 0.2 0.45 0.47 0.42 0.72 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 1.2 1.5 1.7 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -2.9 0 0 0 0 -2.4 0 -2.7 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -3.5 -3.2 -2.5 -3.2 -2.9 -3.5 0 -2.8 0 0.2 -1.4 0.6 0.9 1.9
Cytoplasm -0.7 -3.8 -6.7 -7.7 -4.2 -5.0 -8.2 -5.9 -3.2 -6.2 -0.7 -0.7 -0.5 -2.9
Endoplasmic Reticulum 1.8 2.7 5.1 7.1 0.4 2.6 0 4.0 0 8.3 1.6 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 3.7 5.7 5.0 0 5.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 3.8 3.4 2.1 1.9 2.6 0 1.5 0 0 0 0 0 0