Standard name | |
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Description | Aspartic protease; hyperglycosylated member of the yapsin family of proteases, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; involved in nutrient limitation-induced cleavage of the extracellular inhibitory domain of signaling mucin Msb2p, resulting in activation of the filamentous growth MAPK pathway; involved with other yapsins in the cell wall integrity response; role in KEX2-independent processing of the alpha factor precursor |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 4 | 5 | 11 | 15 | 20 | – | 4 | 6 | 6 | 7 | 0 | 0 | 0 | 0 | 3 | 3 | 10 | 11 | 9 | 1 | 2 | 5 |
Bud | 1 | 2 | 0 | 5 | 4 | 5 | 7 | – | 9 | 9 | 5 | 4 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 3 | 2 | 1 | 4 | 5 |
Bud Neck | 0 | 0 | 10 | 1 | 0 | 0 | 0 | – | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 6 | 10 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | 4 | 26 | 8 | 13 | 22 | 22 | – | 11 | 10 | 9 | 13 | 2 | 15 | 10 | 10 | 12 | 19 | 7 | 3 | 8 | 0 | 2 | 3 |
Cytoplasm | 285 | 206 | 249 | 274 | 303 | 320 | 372 | – | 239 | 263 | 210 | 244 | 18 | 102 | 152 | 75 | 83 | 94 | 146 | 116 | 127 | 15 | 6 | 20 |
Endoplasmic Reticulum | 26 | 35 | 9 | 20 | 32 | 70 | 127 | – | 12 | 29 | 42 | 41 | 8 | 48 | 11 | 3 | 4 | 6 | 128 | 107 | 69 | 16 | 54 | 63 |
Endosome | 0 | 3 | 1 | 0 | 2 | 2 | 5 | – | 0 | 3 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 1 | 0 | 0 |
Golgi | 1 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 9 | 3 | 0 | 2 | 3 | 27 | 27 | – | 34 | 30 | 79 | 37 | 0 | 0 | 0 | 1 | 0 | 1 | 37 | 18 | 53 | 4 | 1 | 4 |
Nucleus | 1 | 1 | 0 | 3 | 3 | 4 | 6 | – | 3 | 3 | 3 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 0 | 0 | 3 | 0 | 1 | – | 2 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 5 | 9 | 5 | 6 | 34 | 35 | 27 | – | 14 | 21 | 6 | 13 | 0 | 0 | 8 | 3 | 2 | 4 | 18 | 12 | 17 | 2 | 2 | 9 |
Unique Cell Count | 327 | 252 | 271 | 304 | 373 | 451 | 553 | 301 | 342 | 332 | 334 | 25 | 149 | 169 | 84 | 92 | 115 | 375 | 292 | 310 | 50 | 78 | 118 | |
Labelled Cell Count | 337 | 268 | 304 | 324 | 408 | 500 | 616 | 328 | 378 | 363 | 369 | 30 | 169 | 182 | 93 | 107 | 134 | 375 | 292 | 310 | 50 | 78 | 118 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.7 | 6.1 | 6.1 | 7.1 | 6.0 | 6.1 | – | 5.4 | 5.4 | 4.6 | 5.1 | 8.0 | 7.6 | 7.0 | 8.3 | 8.4 | 8.1 | – | 10.0 | 10.7 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.9 | 1.6 | 1.8 | 1.2 | 1.1 | – | 1.4 | 1.0 | 0.8 | 1.0 | 1.9 | 1.4 | 1.6 | 2.0 | 2.6 | 2.4 | – | 3.0 | 2.7 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.23 | -0.0 | 0.01 | – | -0.18 | -0.18 | -0.39 | -0.25 | 0.39 | 0.33 | 0.2 | 0.45 | 0.47 | 0.42 | – | 0.72 | 0.82 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 1.2 | 1.5 | 1.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -2.9 | 0 | 0 | 0 | – | 0 | -2.4 | 0 | -2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -3.5 | -3.2 | -2.5 | -3.2 | – | -2.9 | -3.5 | 0 | -2.8 | 0 | 0.2 | -1.4 | 0.6 | 0.9 | 1.9 |
Cytoplasm | -0.7 | -3.8 | -6.7 | -7.7 | – | -4.2 | -5.0 | -8.2 | -5.9 | -3.2 | -6.2 | -0.7 | -0.7 | -0.5 | -2.9 |
Endoplasmic Reticulum | 1.8 | 2.7 | 5.1 | 7.1 | – | 0.4 | 2.6 | 0 | 4.0 | 0 | 8.3 | 1.6 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.1 | 3.7 | – | 5.7 | 5.0 | 0 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 3.8 | 3.4 | 2.1 | – | 1.9 | 2.6 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 |