Standard name
Description Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter mediates export of many different organic anions including oligomycin; homolog of human cystic fibrosis transmembrane receptor (CFTR)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.23 0.34 0.39 0.33 0.45 0.32 – 0.19 0.17 0.14 0.16 0.13 0.14 0.14 0.11 0.16 0.29 0.23 0 0.05 0 0 0 0
Bud 0 0.06 0 0.07 0.07 0.07 – 0.11 0.11 0.12 0.13 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.83 0.82 0.73 0.66 0.64 0.55 – 0.3 0.39 0.28 0.3 0.3 0.91 0.92 0.9 0.85 0.81 0.91 0.9 0.87 0.87 0.84 0.89 0.86
Cytoplasm 0.09 0.11 0.18 0.31 0.21 0.38 – 0.54 0.53 0.54 0.53 0.57 0.07 0.07 0.15 0.21 0.21 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.18 0.16 0.25 0.18 0.22 – 0.25 0.26 0.28 0.31 0.27 0 0 0 0.09 0.1 0.09 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 123 94 160 89 131 121 – 110 81 80 67 54 38 38 27 36 42 8 13 14 13 11 21 20
Bud 13 16 11 18 21 26 – 63 52 68 54 50 0 0 1 6 5 1 2 0 0 1 0 4
Bud Neck 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 7 6 10 9 10 9
Bud Site 0 0 1 1 0 1 – 2 3 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 452 226 300 178 185 210 – 170 182 153 126 122 246 246 213 193 118 32 586 282 411 269 478 412
Cytoplasm 49 29 76 83 60 145 – 308 249 298 220 234 19 19 36 48 31 1 8 3 3 5 2 3
Endoplasmic Reticulum 18 5 8 1 5 4 – 3 8 16 2 6 4 5 4 16 6 2 10 7 12 4 7 15
Endosome 3 0 0 0 1 1 – 0 0 1 0 0 0 0 0 0 0 0 3 0 0 1 0 2
Golgi 19 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 1 0
Mitochondria 68 48 65 68 51 84 – 142 122 154 131 111 2 2 1 20 14 3 5 4 6 11 10 5
Nucleus 0 3 0 1 1 2 – 2 1 1 1 2 2 2 0 3 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 2 – 7 0 3 0 1 0 0 0 0 0 0 0 0 1 1 0 0
Nucleolus 1 0 0 1 0 0 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 8 2 7 14 3 22 – 9 5 6 3 3 0 0 1 0 0 1 0 0 0 0 1 0
SpindlePole 10 3 10 3 4 4 – 3 3 5 4 3 2 1 1 2 5 1 0 0 0 0 0 0
Vac/Vac Membrane 4 0 4 0 2 2 – 6 0 3 2 2 0 0 0 0 0 0 2 0 2 3 2 4
Unique Cell Count 542 274 411 268 288 384 574 469 556 416 411 269 267 237 227 145 35 651 326 474 321 539 482
Labelled Cell Count 768 426 643 458 464 624 825 707 790 610 589 313 313 284 324 222 50 651 326 474 321 539 482


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 9.1 7.2 7.3 7.3 6.4 – 6.1 5.7 5.5 5.4 5.3 10.0 10.0 10.1 7.8 9.2 9.8 9.1 9.2 9.6
Std Deviation (1e-4) 1.4 1.4 1.3 1.5 1.4 1.3 – 1.4 1.1 1.2 1.1 1.0 1.4 1.4 1.5 1.6 1.5 2.2 1.6 1.6 1.5
Intensity Change (Log2) – – – 0.02 0.04 -0.17 – -0.22 -0.32 -0.39 -0.4 -0.42 0.48 0.48 0.5 0.12 0.36 0.45 0.35 0.36 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 1.7 -2.2 – -6.9 -7.2 -8.7 -7.4 -8.4 -7.0 -6.9 -7.5 -6.0 -2.1 -1.9
Bud 2.5 2.9 2.7 – 4.9 4.8 5.4 5.5 5.2 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.8 -2.5 -5.4 – -13.4 -10.2 -14.0 -12.3 -12.4 5.9 6.2 5.1 3.5 2.0 2.4
Cytoplasm 3.8 0.8 6.1 – 11.2 10.6 11.1 10.3 11.4 -4.2 -4.2 -1.1 0.8 0.8 -2.3
Endoplasmic Reticulum 0 -0.2 -1.0 – 0 -0.3 0.9 0 -0.5 0 -0.1 0 3.2 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.1 0.7 2.2 – 3.4 3.7 4.4 5.3 3.9 -6.4 -6.4 -6.2 -2.5 -1.8 -1.1
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 2.6 0 3.0 – -0.2 -0.8 -0.8 0 -1.3 0 0 0 0 0 0
SpindlePole -1.2 -1.0 -1.5 – -2.6 -2.2 -1.9 -1.6 -2.0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9898 6.8682 5.6381 5.8531 4.6673 5.6417 6.5544 8.0425 7.2903 7.3554 6.6335 7.3864 6.0013 7.0196 7.1684 8.0656 6.6662 6.9615
Actin 0.003 0.0003 0.0009 0.0001 0.0002 0.002 0.0865 0.0029 0.0209 0.0018 0.0109 0.0064 0.1665 0.0005 0.0161 0.0002 0.0001 0.0002
Bud 0.0002 0.0002 0.0003 0.0002 0.0001 0.0002 0.0024 0.0004 0.0007 0.0004 0.0012 0.0003 0.0015 0.0002 0.0015 0.0003 0.0002 0.0002
Bud Neck 0.004 0.0026 0.007 0.0072 0.0032 0.0045 0.0085 0.0022 0.0126 0.0245 0.0013 0.0029 0.0015 0.0043 0.0078 0.0095 0.0021 0.0016
Bud Periphery 0.0038 0.0014 0.0041 0.0048 0.0037 0.0043 0.0189 0.0016 0.0077 0.0072 0.0342 0.0052 0.0066 0.0023 0.0028 0.0068 0.0031 0.0062
Bud Site 0.0081 0.0414 0.0208 0.0012 0.001 0.0015 0.0307 0.0711 0.03 0.0027 0.0018 0.0012 0.0144 0.0225 0.0261 0.0024 0.0013 0.0008
Cell Periphery 0.7761 0.7761 0.8118 0.8534 0.9304 0.8898 0.6291 0.7326 0.7942 0.8416 0.8044 0.9085 0.5724 0.7953 0.6834 0.8778 0.9076 0.9131
Cytoplasm 0.1292 0.1574 0.1332 0.0826 0.0519 0.0771 0.0925 0.1613 0.0979 0.0778 0.0452 0.0571 0.0547 0.1681 0.1418 0.091 0.0826 0.069
Cytoplasmic Foci 0.0006 0.0002 0.0002 0.0001 0 0.0004 0.0053 0.001 0.0047 0.0023 0.0084 0.0003 0.0389 0.0001 0.0167 0.0001 0 0.0001
Eisosomes 0.025 0.0024 0.0015 0.0002 0.001 0.002 0.0047 0.013 0.0033 0.0002 0.0012 0.0014 0.0098 0.0008 0.0007 0.0001 0.0001 0.0004
Endoplasmic Reticulum 0.0158 0.0051 0.0098 0.0044 0.0047 0.0089 0.0268 0.0046 0.0074 0.0052 0.0084 0.0095 0.0087 0.0032 0.0034 0.0031 0.0009 0.0029
Endosome 0.0001 0 0.0001 0.0001 0 0.0001 0.0149 0.0001 0.0024 0.0125 0.0148 0.0001 0.0284 0 0.0268 0 0 0
Golgi 0.0003 0.0001 0.0001 0 0 0.0002 0.0089 0.0002 0.0012 0.0008 0.0104 0.0002 0.0093 0.0001 0.0055 0 0 0
Lipid Particles 0.0022 0.002 0.0017 0.0011 0.0003 0.0026 0.0057 0.0011 0.0021 0.0011 0.0137 0.0005 0.01 0.0004 0.0058 0.0005 0.0001 0.0006
Mitochondria 0 0 0 0 0 0 0.0038 0 0.0014 0.0002 0.0246 0.0002 0.0051 0 0.0012 0 0 0
None 0.0245 0.0008 0.0007 0.0001 0.0001 0.0006 0.0116 0.0035 0.0019 0.0019 0.0008 0.0019 0.0088 0.0002 0.035 0.0001 0.0001 0.0016
Nuclear Periphery 0.0012 0.0004 0.0004 0.0003 0.0002 0.0003 0.0211 0.0004 0.0009 0.005 0.0015 0.0003 0.0203 0.0002 0.0022 0.0003 0.0001 0.0001
Nucleolus 0.0001 0.0001 0.0001 0.0001 0 0 0.0012 0.0002 0.0007 0.0005 0.0003 0 0.0003 0 0.0005 0 0 0
Nucleus 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0075 0.0003 0.0008 0.0038 0.0005 0.0001 0.0072 0.0001 0.0008 0.0002 0.0001 0.0001
Peroxisomes 0 0 0 0 0 0 0.0031 0.0001 0.0009 0.0001 0.0061 0 0.0246 0 0.0076 0 0 0
Punctate Nuclear 0.0001 0 0 0 0 0 0.0054 0.0004 0.0033 0.0046 0.0049 0 0.0056 0 0.0005 0 0 0
Vacuole 0.0057 0.0092 0.0071 0.0437 0.0029 0.0053 0.0103 0.003 0.0047 0.0055 0.0048 0.0037 0.0042 0.0017 0.0105 0.0074 0.0017 0.003
Vacuole Periphery 0 0 0 0.0001 0 0 0.0013 0 0.0002 0.0003 0.0007 0.0001 0.0013 0 0.0034 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.6917 37.5953 49.5219 52.6508 41.2987 47.2555 58.031 60.4964 59.7101 52.6428
Translational Efficiency 0.5553 0.5431 0.5147 0.498 0.4269 0.4772 0.4528 0.3806 0.4087 0.3901

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘