Standard name | |
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Description | Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter mediates export of many different organic anions including oligomycin; homolog of human cystic fibrosis transmembrane receptor (CFTR) |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 123 | 94 | 160 | 89 | 131 | 121 | – | 110 | 81 | 80 | 67 | 54 | 38 | 38 | 27 | 36 | 42 | 8 | 13 | 14 | 13 | 11 | 21 | 20 |
Bud | 13 | 16 | 11 | 18 | 21 | 26 | – | 63 | 52 | 68 | 54 | 50 | 0 | 0 | 1 | 6 | 5 | 1 | 2 | 0 | 0 | 1 | 0 | 4 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 6 | 10 | 9 | 10 | 9 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 1 | – | 2 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 452 | 226 | 300 | 178 | 185 | 210 | – | 170 | 182 | 153 | 126 | 122 | 246 | 246 | 213 | 193 | 118 | 32 | 586 | 282 | 411 | 269 | 478 | 412 |
Cytoplasm | 49 | 29 | 76 | 83 | 60 | 145 | – | 308 | 249 | 298 | 220 | 234 | 19 | 19 | 36 | 48 | 31 | 1 | 8 | 3 | 3 | 5 | 2 | 3 |
Endoplasmic Reticulum | 18 | 5 | 8 | 1 | 5 | 4 | – | 3 | 8 | 16 | 2 | 6 | 4 | 5 | 4 | 16 | 6 | 2 | 10 | 7 | 12 | 4 | 7 | 15 |
Endosome | 3 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 2 |
Golgi | 19 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 68 | 48 | 65 | 68 | 51 | 84 | – | 142 | 122 | 154 | 131 | 111 | 2 | 2 | 1 | 20 | 14 | 3 | 5 | 4 | 6 | 11 | 10 | 5 |
Nucleus | 0 | 3 | 0 | 1 | 1 | 2 | – | 2 | 1 | 1 | 1 | 2 | 2 | 2 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 2 | – | 7 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 8 | 2 | 7 | 14 | 3 | 22 | – | 9 | 5 | 6 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 10 | 3 | 10 | 3 | 4 | 4 | – | 3 | 3 | 5 | 4 | 3 | 2 | 1 | 1 | 2 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 0 | 4 | 0 | 2 | 2 | – | 6 | 0 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 2 | 4 |
Unique Cell Count | 542 | 274 | 411 | 268 | 288 | 384 | 574 | 469 | 556 | 416 | 411 | 269 | 267 | 237 | 227 | 145 | 35 | 651 | 326 | 474 | 321 | 539 | 482 | |
Labelled Cell Count | 768 | 426 | 643 | 458 | 464 | 624 | 825 | 707 | 790 | 610 | 589 | 313 | 313 | 284 | 324 | 222 | 50 | 651 | 326 | 474 | 321 | 539 | 482 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 9.1 | 7.2 | 7.3 | 7.3 | 6.4 | – | 6.1 | 5.7 | 5.5 | 5.4 | 5.3 | 10.0 | 10.0 | 10.1 | 7.8 | 9.2 | 9.8 | 9.1 | 9.2 | 9.6 |
Std Deviation (1e-4) | 1.4 | 1.4 | 1.3 | 1.5 | 1.4 | 1.3 | – | 1.4 | 1.1 | 1.2 | 1.1 | 1.0 | 1.4 | 1.4 | 1.5 | 1.6 | 1.5 | 2.2 | 1.6 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.04 | -0.17 | – | -0.22 | -0.32 | -0.39 | -0.4 | -0.42 | 0.48 | 0.48 | 0.5 | 0.12 | 0.36 | 0.45 | 0.35 | 0.36 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.5 | 1.7 | -2.2 | – | -6.9 | -7.2 | -8.7 | -7.4 | -8.4 | -7.0 | -6.9 | -7.5 | -6.0 | -2.1 | -1.9 |
Bud | 2.5 | 2.9 | 2.7 | – | 4.9 | 4.8 | 5.4 | 5.5 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.8 | -2.5 | -5.4 | – | -13.4 | -10.2 | -14.0 | -12.3 | -12.4 | 5.9 | 6.2 | 5.1 | 3.5 | 2.0 | 2.4 |
Cytoplasm | 3.8 | 0.8 | 6.1 | – | 11.2 | 10.6 | 11.1 | 10.3 | 11.4 | -4.2 | -4.2 | -1.1 | 0.8 | 0.8 | -2.3 |
Endoplasmic Reticulum | 0 | -0.2 | -1.0 | – | 0 | -0.3 | 0.9 | 0 | -0.5 | 0 | -0.1 | 0 | 3.2 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.1 | 0.7 | 2.2 | – | 3.4 | 3.7 | 4.4 | 5.3 | 3.9 | -6.4 | -6.4 | -6.2 | -2.5 | -1.8 | -1.1 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2.6 | 0 | 3.0 | – | -0.2 | -0.8 | -0.8 | 0 | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.2 | -1.0 | -1.5 | – | -2.6 | -2.2 | -1.9 | -1.6 | -2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9898 | 6.8682 | 5.6381 | 5.8531 | 4.6673 | 5.6417 | 6.5544 | 8.0425 | 7.2903 | 7.3554 | 6.6335 | 7.3864 | 6.0013 | 7.0196 | 7.1684 | 8.0656 | 6.6662 | 6.9615 |
Actin | 0.003 | 0.0003 | 0.0009 | 0.0001 | 0.0002 | 0.002 | 0.0865 | 0.0029 | 0.0209 | 0.0018 | 0.0109 | 0.0064 | 0.1665 | 0.0005 | 0.0161 | 0.0002 | 0.0001 | 0.0002 |
Bud | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0024 | 0.0004 | 0.0007 | 0.0004 | 0.0012 | 0.0003 | 0.0015 | 0.0002 | 0.0015 | 0.0003 | 0.0002 | 0.0002 |
Bud Neck | 0.004 | 0.0026 | 0.007 | 0.0072 | 0.0032 | 0.0045 | 0.0085 | 0.0022 | 0.0126 | 0.0245 | 0.0013 | 0.0029 | 0.0015 | 0.0043 | 0.0078 | 0.0095 | 0.0021 | 0.0016 |
Bud Periphery | 0.0038 | 0.0014 | 0.0041 | 0.0048 | 0.0037 | 0.0043 | 0.0189 | 0.0016 | 0.0077 | 0.0072 | 0.0342 | 0.0052 | 0.0066 | 0.0023 | 0.0028 | 0.0068 | 0.0031 | 0.0062 |
Bud Site | 0.0081 | 0.0414 | 0.0208 | 0.0012 | 0.001 | 0.0015 | 0.0307 | 0.0711 | 0.03 | 0.0027 | 0.0018 | 0.0012 | 0.0144 | 0.0225 | 0.0261 | 0.0024 | 0.0013 | 0.0008 |
Cell Periphery | 0.7761 | 0.7761 | 0.8118 | 0.8534 | 0.9304 | 0.8898 | 0.6291 | 0.7326 | 0.7942 | 0.8416 | 0.8044 | 0.9085 | 0.5724 | 0.7953 | 0.6834 | 0.8778 | 0.9076 | 0.9131 |
Cytoplasm | 0.1292 | 0.1574 | 0.1332 | 0.0826 | 0.0519 | 0.0771 | 0.0925 | 0.1613 | 0.0979 | 0.0778 | 0.0452 | 0.0571 | 0.0547 | 0.1681 | 0.1418 | 0.091 | 0.0826 | 0.069 |
Cytoplasmic Foci | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.0053 | 0.001 | 0.0047 | 0.0023 | 0.0084 | 0.0003 | 0.0389 | 0.0001 | 0.0167 | 0.0001 | 0 | 0.0001 |
Eisosomes | 0.025 | 0.0024 | 0.0015 | 0.0002 | 0.001 | 0.002 | 0.0047 | 0.013 | 0.0033 | 0.0002 | 0.0012 | 0.0014 | 0.0098 | 0.0008 | 0.0007 | 0.0001 | 0.0001 | 0.0004 |
Endoplasmic Reticulum | 0.0158 | 0.0051 | 0.0098 | 0.0044 | 0.0047 | 0.0089 | 0.0268 | 0.0046 | 0.0074 | 0.0052 | 0.0084 | 0.0095 | 0.0087 | 0.0032 | 0.0034 | 0.0031 | 0.0009 | 0.0029 |
Endosome | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0149 | 0.0001 | 0.0024 | 0.0125 | 0.0148 | 0.0001 | 0.0284 | 0 | 0.0268 | 0 | 0 | 0 |
Golgi | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0089 | 0.0002 | 0.0012 | 0.0008 | 0.0104 | 0.0002 | 0.0093 | 0.0001 | 0.0055 | 0 | 0 | 0 |
Lipid Particles | 0.0022 | 0.002 | 0.0017 | 0.0011 | 0.0003 | 0.0026 | 0.0057 | 0.0011 | 0.0021 | 0.0011 | 0.0137 | 0.0005 | 0.01 | 0.0004 | 0.0058 | 0.0005 | 0.0001 | 0.0006 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0.0038 | 0 | 0.0014 | 0.0002 | 0.0246 | 0.0002 | 0.0051 | 0 | 0.0012 | 0 | 0 | 0 |
None | 0.0245 | 0.0008 | 0.0007 | 0.0001 | 0.0001 | 0.0006 | 0.0116 | 0.0035 | 0.0019 | 0.0019 | 0.0008 | 0.0019 | 0.0088 | 0.0002 | 0.035 | 0.0001 | 0.0001 | 0.0016 |
Nuclear Periphery | 0.0012 | 0.0004 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0211 | 0.0004 | 0.0009 | 0.005 | 0.0015 | 0.0003 | 0.0203 | 0.0002 | 0.0022 | 0.0003 | 0.0001 | 0.0001 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0012 | 0.0002 | 0.0007 | 0.0005 | 0.0003 | 0 | 0.0003 | 0 | 0.0005 | 0 | 0 | 0 |
Nucleus | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0075 | 0.0003 | 0.0008 | 0.0038 | 0.0005 | 0.0001 | 0.0072 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0001 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0031 | 0.0001 | 0.0009 | 0.0001 | 0.0061 | 0 | 0.0246 | 0 | 0.0076 | 0 | 0 | 0 |
Punctate Nuclear | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0054 | 0.0004 | 0.0033 | 0.0046 | 0.0049 | 0 | 0.0056 | 0 | 0.0005 | 0 | 0 | 0 |
Vacuole | 0.0057 | 0.0092 | 0.0071 | 0.0437 | 0.0029 | 0.0053 | 0.0103 | 0.003 | 0.0047 | 0.0055 | 0.0048 | 0.0037 | 0.0042 | 0.0017 | 0.0105 | 0.0074 | 0.0017 | 0.003 |
Vacuole Periphery | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0013 | 0 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0013 | 0 | 0.0034 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 39.6917 | 37.5953 | 49.5219 | 52.6508 | 41.2987 | 47.2555 | 58.031 | 60.4964 | 59.7101 | 52.6428 |
Translational Efficiency | 0.5553 | 0.5431 | 0.5147 | 0.498 | 0.4269 | 0.4772 | 0.4528 | 0.3806 | 0.4087 | 0.3901 |
Dataset | Hit |
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Protein Abundance | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |