Standard name
Description Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats and FFAT motif; interacts with ER anchor Scs2p at the nucleus-vacuole junction; regulated by sterol binding; SWH1 has a paralog, OSH2, that arose from the whole genome duplication

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.11 0.06 0.07 0 0 0 0 0 0.06 0 0 0 0.11 0.13 0.12 0 0 0 0.06 0.10 0.10
Bud 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.10 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.20 0 0.35 0.53 0.51 0.37 0.34 0.32 0.09 0.07 0.13 0.26 0.33 0.37 0.12 0.07 0.18 0.29 0.37 0.47 0.35 0.27 0.24
Golgi 0 0.05 0.16 0.06 0.08 0 0 0 0 0 0 0.07 0.15 0.18 0.62 0.69 0.59 0.35 0.38 0.20 0.27 0.32 0.38
Mitochondria 0.80 0.87 0.43 0.26 0.21 0.52 0.53 0.47 0.79 0.80 0.70 0.65 0.52 0.34 0.12 0.07 0.10 0.05 0.05 0.07 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.09 0 0.07 0.08 0 0.06 0.05 0.07 0.11 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0 0.06 0.07 0.10 0.10 0.07 0.05 0 0 0.06 0.21 0.20 0.21 0 0 0 0.25 0.11 0.19 0.21 0.24 0.21
SpindlePole 0 0 0.10 0.22 0.24 0.11 0.15 0.14 0.05 0.07 0.09 0 0 0.08 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0.05 0 0.08 0 0 0.06 0 0.05 0.07 0.09 0.09 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 3 26 12 18 4 9 14 5 10 17 10 6 5 14 12 14 7 2 3 15 44 50
Bud 3 2 3 1 3 5 12 13 13 12 24 10 9 3 0 3 0 0 0 1 0 3 1
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 1 0 0 0 1 2 0 1 2 2 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0
Cytoplasm 7 1 5 9 9 11 14 16 9 11 13 11 9 17 14 8 8 0 0 0 1 0 1
Endoplasmic Reticulum 5 13 15 6 4 9 8 6 5 8 9 9 6 4 6 4 4 0 0 3 2 6 7
Endosome 50 3 82 107 141 110 144 136 23 18 37 114 144 179 15 7 21 76 57 127 90 121 126
Golgi 5 6 38 12 21 3 4 13 3 1 11 31 66 89 80 65 70 90 58 56 71 144 195
Mitochondria 206 108 99 52 58 154 225 197 194 212 205 286 230 165 15 7 12 14 7 18 12 12 16
Nucleus 0 1 0 0 0 1 1 4 2 3 1 1 1 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 13 9 2 2 4 6 7 9 6 10 4 10 7 8 1 0 3 1 1 1 1 0 0
Nucleolus 30 11 4 15 21 12 27 23 17 30 14 16 16 15 5 3 3 0 0 0 0 1 0
Peroxisomes 16 3 15 15 27 30 31 21 8 6 17 94 89 102 1 0 1 64 17 52 56 106 107
SpindlePole 5 2 23 44 66 31 62 59 13 18 25 19 16 38 6 2 5 1 0 3 3 5 5
Vac/Vac Membrane 8 5 15 7 8 16 16 34 11 5 17 12 24 33 11 8 15 1 4 3 1 1 0
Unique Cell Count 256 124 232 202 274 294 423 423 246 265 293 440 442 485 129 94 119 263 153 274 262 451 521
Labelled Cell Count 359 168 328 282 381 393 562 545 310 347 396 623 624 658 169 120 156 263 153 274 262 451 521

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Punctate Nuclear

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 8.4 8.2 7.7 7.9 6.6 6.7 7.0 6.0 5.5 6.3 9.0 9.2 10.3 15.4 16.1 15.0 8.4 8.2 8.8
Std Deviation (1e-4) 1.1 1.8 1.2 1.0 1.1 1.2 1.2 1.2 1.1 1.1 1.1 1.2 2.2 3.6 3.2 3.3 3.0 2.4 1.2 1.5
Intensity Change (Log2) -0.1 -0.05 -0.32 -0.3 -0.24 -0.46 -0.58 -0.39 0.13 0.16 0.32 0.91 0.97 0.87 0.03 0.0 0.1

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.9 -1.8 -4.8 -4.9 -4.0 -4.1 -3.2 -2.2 -4.9 -5.7 -6.3 -0.1 0.4 0.2
Bud 0 0 0 1.3 1.4 2.4 2.1 3.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum -1.7 -3.0 -1.9 -3.0 -3.5 -2.4 -1.8 -1.8 -2.9 -3.6 -4.4 -0.7 -0.8 -1.2
Endosome 3.7 3.6 0.5 -0.3 -0.8 -6.9 -7.9 -6.2 -2.6 -0.7 0.4 -4.9 -5.1 -3.4
Golgi -3.4 -3.0 -6.5 -7.7 -6.1 -5.9 -6.6 -4.9 -3.8 -0.5 0.6 8.9 9.3 8.2
Mitochondria -3.7 -5.2 2.2 2.6 1.0 8.1 8.6 6.3 5.6 2.3 -2.2 -6.1 -6.2 -6.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 2.1 0 0 0 0 0 0 0
Nucleolus 2.9 3.1 1.6 2.7 2.3 2.8 4.2 1.9 1.4 1.4 1.1 0 0 0
Peroxisomes 0.4 1.4 1.5 0.4 -0.8 -1.6 -2.3 -0.3 5.0 4.7 4.9 -2.5 0 -2.4
SpindlePole 3.4 4.2 0.2 1.7 1.5 -1.9 -1.3 -0.5 -2.8 -3.3 -0.9 -1.8 -2.4 -1.9
Vacuole 0 -1.8 -1.0 0 -0.4 -1.0 0 -0.7 0 0 0 0 0 0