Standard name
Description Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.07 0.08 0.07 0.15 0.13 0.12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.88 0.86 0.8 0.94 0.92 0.86 0.81 0.84 0.78 0.8 0.71 0.97 0.99 0.98 0.84 0.8 0.85 0.7 0.64 0.59 0.38 0.43 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.14 0.21 0.13 0.35 0.36 0.36 0 0 0 0 0 0 0 0 0 0 0.05 0
Nucleus 0.06 0.05 0.14 0 0.05 0.07 0.07 0.07 0.06 0 0.05 0 0 0 0 0.09 0 0.06 0.06 0.11 0.05 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.11 0.08 0.11 0.05
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.09 0 0 0 0.13 0.08 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 2 1 0 0 4 4
Bud 1 3 1 0 1 2 4 4 4 0 6 1 0 0 1 2 1 23 16 7 13 27 20
Bud Neck 2 0 0 0 1 1 2 5 5 1 1 0 0 0 0 0 0 1 3 0 1 4 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 0 0 1 0 0 0 0 1 0 10 14 5 1 1 0 0 1 1
Cytoplasm 300 176 74 64 59 85 98 162 111 47 85 229 266 291 223 140 166 248 139 62 32 95 89
Endoplasmic Reticulum 0 0 0 0 1 0 2 0 0 0 0 2 0 2 16 9 6 0 0 0 1 2 0
Endosome 4 6 1 0 0 0 0 0 1 0 0 0 0 0 5 2 6 5 3 0 3 5 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 1 1 0 0 0 0 0
Mitochondria 7 4 4 5 1 14 26 25 49 21 43 2 0 2 7 6 3 3 4 3 3 11 4
Nucleus 20 11 13 1 3 7 9 13 8 1 6 0 1 0 11 15 7 21 13 11 4 10 5
Nuclear Periphery 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 1 4 3 1 0 2 3 2 2 1 0 1 0 0 6 3 2 9 5 2 3 6 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 2 0 2 0 0 0 1 2 0 0 1 0 0 0 0 1 1 26 20 11 6 23 9
Vac/Vac Membrane 11 10 2 0 0 1 1 1 3 0 4 0 2 3 29 16 18 5 3 3 11 17 18
Unique Cell Count 339 205 93 68 64 99 121 194 142 59 119 235 268 296 266 175 196 356 217 106 86 220 173
Labelled Cell Count 348 214 100 71 67 113 147 216 183 71 146 235 270 298 311 211 217 356 217 106 86 220 173


Bud, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.1 4.7 4.3 5.1 3.8 4.0 4.2 3.5 3.4 3.5 4.5 4.9 4.8 8.3 8.6 8.4 4.9 4.8 5.3
Std Deviation (1e-4) 0.9 1.1 0.8 0.9 1.2 0.8 1.1 1.1 0.5 1.0 0.8 1.0 1.2 1.2 2.4 2.9 2.9 1.4 1.6 2.1
Intensity Change (Log2) -0.13 0.12 -0.3 -0.23 -0.16 -0.44 -0.5 -0.42 -0.06 0.06 0.03 0.82 0.87 0.83 0.04 0.02 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.6 2.2 1.2 0.3 0.8 -0.3 0 -1.4 5.5 7.0 6.6 0.9 0.1 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -2.8 -1.9 -1.6 -1.6 -2.0 -2.2 -2.6 -2.3 0 0 0 -3.3 -1.4 -3.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 2.6 2.2 2.2