Standard name
Human Ortholog
Description Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.2 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0.05 0 0.08 – 0.09 0.09 0 0.09 – 0 0 0.06 0 0.06 0 0 0 0 0 0
Cytoplasm 1.0 1.0 – 0.95 0.97 0.95 0.88 – 0.8 0.78 0.8 0.79 – 0.98 0.99 0.93 0.94 0.97 0.95 0.95 0.96 0.65 0.93 0.9
Endoplasmic Reticulum 0 0 – 0 0 0 0.11 – 0.13 0.11 0 0.12 – 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0 – 0.05 0.06 0.19 0.1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 1 0 1 6 – 8 9 1 4 – 0 0 0 1 1 0 0 0 0 0 0
Bud 0 0 – 0 1 0 1 – 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 – 2 10 11 29 – 16 27 2 18 – 4 4 18 10 16 0 0 0 0 0 0
Cytoplasm 45 62 – 109 208 256 306 – 144 246 99 165 – 100 156 297 243 246 67 82 15 2 85 116
Endoplasmic Reticulum 0 0 – 3 6 8 38 – 24 35 4 26 – 1 1 15 13 6 0 1 0 0 0 1
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 – 1 0 0 1 – 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 2 3 – 9 19 23 21 – 0 0 1 1 3 0 0 0 0 0 0
Nucleus 0 0 – 2 2 2 1 – 3 0 3 0 – 3 2 4 5 1 0 1 0 0 0 4
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 2 8 2 2 – 0 0 16 5 5 1 0 0 0 1 2
Unique Cell Count 45 62 115 214 269 348 181 315 123 208 102 157 321 258 254 71 87 16 4 92 130
Labelled Cell Count 46 62 118 227 280 385 206 345 135 237 108 163 352 279 278 71 87 16 4 92 130


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 12.6 – 12.5 14.5 13.4 13.4 – 11.6 11.9 10.2 11.3 – 14.7 13.6 21.7 21.2 20.1 – 13.5 13.4
Std Deviation (1e-4) 2.4 2.4 – 2.2 1.8 2.2 2.1 – 2.5 2.2 2.1 2.3 – 2.6 2.0 4.9 5.1 5.0 – 2.5 2.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.2413 10.898 9.4229 8.8471 7.7822 9.3144 6.8058 10.0667 8.5321 7.3547 8.4326 9.3986 5.5135 7.9457 6.7063 6.9761 6.6267 7.0389
Actin 0.0134 0.0012 0.0098 0.0003 0.0064 0.0015 0.035 0.0003 0.0098 0.0117 0 0.0034 0.032 0.0004 0.0169 0.0014 0.0001 0.0012
Bud 0.0023 0.0083 0.0104 0.019 0.0123 0.0071 0.0009 0.0004 0.0006 0.003 0.0002 0.0004 0.0006 0.0002 0.0004 0.0007 0.0001 0.0002
Bud Neck 0.0012 0.0004 0.0021 0.001 0.0012 0.0019 0.0037 0.0001 0.004 0.0012 0.0003 0.0009 0.0006 0.0001 0.0004 0.0006 0.0008 0.0006
Bud Periphery 0.0016 0.0025 0.0029 0.0089 0.0099 0.0024 0.0012 0.0002 0.0007 0.0022 0 0.0005 0.0008 0.0001 0.0003 0.0002 0 0.0001
Bud Site 0.0084 0.008 0.0101 0.0078 0.0023 0.0006 0.0086 0.0007 0.003 0.0121 0 0.0002 0.0025 0.0004 0.0016 0.0002 0 0.0001
Cell Periphery 0.0008 0.0006 0.0005 0.0005 0.0005 0.0003 0.0008 0.0002 0.0006 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.6586 0.9412 0.8704 0.8064 0.682 0.8587 0.701 0.9639 0.8462 0.6659 0.9413 0.9007 0.8527 0.9753 0.8985 0.9424 0.9809 0.932
Cytoplasmic Foci 0.0518 0.0056 0.0224 0.011 0.0283 0.0127 0.0276 0.0066 0.0166 0.0443 0.0071 0.0082 0.0123 0.004 0.0149 0.0091 0.006 0.0076
Eisosomes 0.0004 0 0.0001 0 0.0001 0 0.0004 0 0.0003 0.0001 0 0.0001 0.0003 0 0.0005 0 0 0
Endoplasmic Reticulum 0.0228 0.0079 0.0055 0.0081 0.0042 0.0246 0.0273 0.0088 0.0075 0.0041 0.0019 0.0308 0.0178 0.0048 0.0052 0.0035 0.0021 0.0099
Endosome 0.091 0.0106 0.0325 0.0593 0.0996 0.0422 0.0584 0.0112 0.0303 0.097 0.0293 0.0276 0.0338 0.0049 0.0276 0.0191 0.0063 0.0232
Golgi 0.0234 0.0016 0.0055 0.0039 0.0557 0.0089 0.0135 0.001 0.0098 0.0165 0.0002 0.0037 0.0084 0.0006 0.0023 0.0008 0.0002 0.0038
Lipid Particles 0.0136 0.0001 0.0024 0.0082 0.0162 0.0021 0.0207 0.0002 0.0092 0.0236 0 0.0011 0.0102 0 0.0034 0.0001 0 0.0083
Mitochondria 0.0399 0.0015 0.0069 0.0018 0.0528 0.0183 0.0199 0.0003 0.0103 0.0069 0.0001 0.0112 0.0095 0.0002 0.0015 0.0005 0.0001 0.0015
None 0.0179 0.0026 0.0039 0.0009 0.0012 0.0012 0.0074 0.0012 0.0204 0.0019 0.0004 0.0007 0.0027 0.001 0.0033 0.0006 0.0007 0.0007
Nuclear Periphery 0.0063 0.0008 0.0012 0.0025 0.0021 0.0031 0.0226 0.0005 0.0057 0.0028 0.0005 0.0036 0.0022 0.0005 0.0042 0.0011 0.0003 0.0038
Nucleolus 0.0022 0.0006 0.0003 0.0009 0.0003 0.001 0.0022 0 0.0008 0.0003 0 0.0001 0.0004 0 0.0016 0 0 0.0001
Nucleus 0.004 0.0016 0.0016 0.0026 0.0013 0.0042 0.0065 0.0005 0.0026 0.0019 0.0008 0.001 0.0009 0.0006 0.0028 0.0012 0.0005 0.001
Peroxisomes 0.0141 0.0001 0.0016 0.0004 0.0042 0.0005 0.0099 0 0.0075 0.051 0 0.0002 0.0023 0 0.0016 0.0001 0 0.0007
Punctate Nuclear 0.0077 0.0002 0.0007 0.0004 0.0005 0.0003 0.0084 0.0001 0.0055 0.004 0 0.0002 0.0006 0 0.0064 0.0001 0 0.0022
Vacuole 0.0147 0.0041 0.0078 0.0505 0.0135 0.0059 0.0188 0.0034 0.0072 0.0419 0.0141 0.003 0.0071 0.0063 0.0051 0.0166 0.0013 0.0019
Vacuole Periphery 0.0038 0.0006 0.0015 0.0058 0.0055 0.0023 0.0051 0.0005 0.0014 0.0072 0.0037 0.0022 0.0022 0.0004 0.0012 0.0015 0.0004 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.6712 11.2793 12.1361 17.4287 17.1772 18.9158 21.8111 24.2464 26.1164 19.856
Translational Efficiency 1.4167 1.7237 1.6031 1.3575 0.9892 1.1165 1.1944 1.0077 1.1861 1.2203

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1766 1006 543 498 1244 1571 533 1566 3010 2577 1076 2064

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 990.58 1003.61 1677.99 1464.84 1049.50 1077.94 1414.81 1513.98 1014.93 1048.92 1547.62 1502.12
Standard Deviation 157.71 162.02 250.44 241.36 141.14 158.16 242.02 227.89 153.84 163.74 279.25 232.17
Intensity Change Log 2 — 0.018853 0.760389 0.564398 — 0.038575 0.430906 0.528644 — 0.029032 0.600288 0.546115

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000161 0.000367 0.000407 0.000789 0.000208 0.000386 0.000287 0.000525 0.000181 0.000378 0.000347 0.000589
Bud Neck 0.000836 0.000660 0.003494 0.001758 0.000335 0.000582 0.002046 0.001102 0.000629 0.000612 0.002777 0.001260
Bud Site 0.026645 0.024679 0.012483 0.030240 0.015524 0.018547 0.011200 0.015095 0.022048 0.020941 0.011847 0.018749
Cell Periphery 0.000268 0.000165 0.000747 0.001603 0.000163 0.000140 0.000300 0.001215 0.000225 0.000150 0.000526 0.001309
Cytoplasm 0.765011* 0.830517* 0.647405* 0.862780* 0.802554* 0.806375* 0.530660* 0.910239* 0.780527* 0.815800* 0.589575* 0.898788*
Cytoplasmic Foci 0.129236 0.090639 0.034775 0.021110 0.128265 0.107518 0.048567 0.012962 0.128835 0.100929 0.041607 0.014928
Eisosomes 0.000009 0.000006 0.000007 0.000006 0.000005 0.000007 0.000018 0.000004 0.000007 0.000006 0.000012 0.000004
Endoplasmic Reticulum 0.005569 0.001827 0.003439 0.000176 0.005064 0.003107 0.005007 0.000269 0.005360 0.002607 0.004216 0.000246
Endosome 0.004430 0.005928 0.011643 0.004182 0.006444 0.008659 0.014672 0.001203 0.005263 0.007593 0.013144 0.001922
Golgi 0.011695 0.007668 0.008121 0.003601 0.010493 0.011083 0.002569 0.003580 0.011198 0.009750 0.005371 0.003585
Lipid Particles 0.000543 0.000590 0.005420 0.005496 0.000693 0.000721 0.003958 0.003350 0.000605 0.000670 0.004696 0.003868
Mitochondria 0.001359 0.000262 0.000033 0.000558 0.000267 0.001623 0.000042 0.000195 0.000908 0.001092 0.000037 0.000282
Mitotic Spindle 0.000412 0.000432 0.003699 0.003109 0.000773 0.001773 0.000779 0.001186 0.000561 0.001250 0.002253 0.001650
None 0.001968 0.000468 0.000251 0.000130 0.000565 0.000736 0.000850 0.000255 0.001388 0.000631 0.000548 0.000225
Nuclear Periphery 0.000019 0.000049 0.000284 0.000031 0.000023 0.000037 0.000367 0.000056 0.000021 0.000042 0.000325 0.000050
Nuclear Periphery Foci 0.000288 0.000603 0.007291 0.001043 0.000630 0.000473 0.003457 0.001026 0.000430 0.000524 0.005392 0.001030
Nucleolus 0.000171 0.000095 0.000114 0.000072 0.000029 0.000071 0.000185 0.000096 0.000113 0.000080 0.000149 0.000090
Nucleus 0.004503 0.005294 0.011148 0.002678 0.002422 0.002853 0.021622 0.004485 0.003643 0.003806 0.016336 0.004049
Peroxisomes 0.000347 0.000615 0.000322 0.001075 0.000238 0.000633 0.000395 0.000396 0.000302 0.000626 0.000358 0.000560
Vacuole 0.021914 0.023352 0.242846* 0.057999 0.016900 0.029811 0.347591* 0.041120 0.019842 0.027290 0.294732* 0.045192
Vacuole Periphery 0.024615 0.005785 0.006072 0.001567 0.008407 0.004865 0.005427 0.001642 0.017916 0.005224 0.005752 0.001624

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.71 -6.40 -4.62 -2.53 -2.65 -2.08 -2.32 -3.02 -1.19 -2.03 -2.85 -6.38 -4.80 -2.14 -2.58
Bud Neck 1.40 -10.35 -5.91 -7.68 6.06 -4.26 -10.13 -9.09 -5.83 4.80 0.21 -13.41 -7.39 -8.72 8.97
Bud Site 0.97 7.95 -1.32 -1.91 -5.95 -2.11 2.29 0.08 2.03 -2.08 0.88 7.87 2.13 1.33 -4.79
Cell Periphery 6.25 -4.42 -6.85 -7.42 -3.85 1.82 -4.12 -5.88 -6.01 -4.99 7.02 -5.28 -7.49 -8.04 -5.05
Cytoplasm -7.54 8.50 -8.18 -1.70 -13.05 -0.41 17.39 -13.71 -13.92 -25.41 -5.64 17.92 -19.83 -13.29 -28.83
Cytoplasmic Foci 6.92 21.71 24.85 15.09 4.97 3.47 14.63 24.03 24.64 12.41 6.89 25.36 36.72 29.47 14.23
Eisosomes 3.73 2.10 2.18 -0.64 0.62 -3.12 -5.77 1.54 3.89 6.06 0.87 -4.30 4.81 3.50 6.50
Endoplasmic Reticulum 4.33 2.28 6.90 4.49 4.27 1.90 0.27 6.51 3.87 4.94 3.82 1.78 9.24 5.00 6.45
Endosome -2.61 -9.26 0.41 1.45 5.19 -2.76 -7.81 11.14 10.74 14.24 -4.55 -12.25 8.41 10.05 16.42
Golgi 4.31 3.95 6.24 2.88 2.81 -0.62 15.02 7.38 6.11 -1.02 1.92 10.43 10.09 6.61 2.03
Lipid Particles -0.76 -8.36 -4.37 -4.33 -0.20 -0.36 -6.92 -9.39 -9.08 1.01 -1.15 -10.87 -9.35 -9.09 1.44
Mitochondria 7.61 9.91 2.71 -0.78 -1.58 -2.22 4.25 0.83 2.34 -1.77 -0.49 10.61 5.20 2.17 -2.37
Mitotic Spindle -0.21 -5.24 -1.21 -1.20 0.60 -1.40 0.24 -0.38 0.89 -0.59 -1.78 -4.80 -1.46 -0.37 1.29
None 4.37 5.13 5.49 3.93 2.69 -0.58 -0.38 2.60 1.77 0.91 2.86 2.43 5.74 2.40 1.01
Nuclear Periphery -0.91 -5.68 -1.17 0.46 5.28 -0.75 -3.07 -1.05 -0.50 2.68 -1.22 -5.23 -1.24 -0.27 4.42
Nuclear Periphery Foci -2.29 -9.86 -7.11 -3.03 8.63 1.03 -7.72 -4.43 -3.86 6.51 -0.88 -12.28 -8.92 -4.84 10.59
Nucleolus 0.90 0.47 1.29 0.65 3.57 -1.53 -5.68 -8.94 -0.22 3.08 0.42 -0.90 0.49 0.01 4.03
Nucleus -0.61 -6.84 1.95 1.66 8.63 -1.57 -7.72 -4.55 -3.80 6.52 -0.29 -9.76 -1.75 -1.21 8.94
Peroxisomes -2.09 0.25 -4.53 -2.34 -4.51 -4.78 -3.39 -3.55 3.01 0.31 -4.70 -2.21 -5.29 0.92 -3.53
Vacuole -0.59 -18.10 -8.95 -8.44 13.16 -5.62 -24.29 -11.13 -5.31 22.02 -4.28 -29.66 -13.46 -9.54 25.80
Vacuole Periphery 9.65 10.02 12.61 5.16 6.01 3.22 2.61 6.36 6.09 4.55 10.49 10.13 13.90 7.72 7.20
Standard name
Human Ortholog
Description Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Cki1

Cki1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cki1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available