Standard name
Description Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.2 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0.05 0 0.08 – 0.09 0.09 0 0.09 – 0 0 0.06 0 0.06 0 0 0 0 0 0
Cytoplasm 1.0 1.0 – 0.95 0.97 0.95 0.88 – 0.8 0.78 0.8 0.79 – 0.98 0.99 0.93 0.94 0.97 0.95 0.95 0.96 0.65 0.93 0.9
Endoplasmic Reticulum 0 0 – 0 0 0 0.11 – 0.13 0.11 0 0.12 – 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0 – 0.05 0.06 0.19 0.1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 1 0 1 6 – 8 9 1 4 – 0 0 0 1 1 0 0 0 0 0 0
Bud 0 0 – 0 1 0 1 – 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 – 2 10 11 29 – 16 27 2 18 – 4 4 18 10 16 0 0 0 0 0 0
Cytoplasm 45 62 – 109 208 256 306 – 144 246 99 165 – 100 156 297 243 246 67 82 15 2 85 116
Endoplasmic Reticulum 0 0 – 3 6 8 38 – 24 35 4 26 – 1 1 15 13 6 0 1 0 0 0 1
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 – 1 0 0 1 – 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 2 3 – 9 19 23 21 – 0 0 1 1 3 0 0 0 0 0 0
Nucleus 0 0 – 2 2 2 1 – 3 0 3 0 – 3 2 4 5 1 0 1 0 0 0 4
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 2 8 2 2 – 0 0 16 5 5 1 0 0 0 1 2
Unique Cell Count 45 62 115 214 269 348 181 315 123 208 102 157 321 258 254 71 87 16 4 92 130
Labelled Cell Count 46 62 118 227 280 385 206 345 135 237 108 163 352 279 278 71 87 16 4 92 130


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 12.6 – 12.5 14.5 13.4 13.4 – 11.6 11.9 10.2 11.3 – 14.7 13.6 21.7 21.2 20.1 – 13.5 13.4
Std Deviation (1e-4) 2.4 2.4 – 2.2 1.8 2.2 2.1 – 2.5 2.2 2.1 2.3 – 2.6 2.0 4.9 5.1 5.0 – 2.5 2.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cki1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available