Standard name
Description Endonuclease involved in processing DNA; acts during recombination, repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in complex with Slx1p; involved in interstrand cross-link repair, Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress; FANCP-related factor

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.51 0.48 0.8 0.65 0.51 0.55 0.58 0.41 0.45 0.41 0.45 0.41 0.55 0.64 0.45 0.37 0.39 0.31 0.25 0.32 0.3 0.36 0.51
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 0 0 0 0.06 0 0.1 0 0.1 0.11 0.1 0.08 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.17 0 0.15 0.06 0.27 0.29 0.58 0.49 0.55 0.36 0.27 0.1 0.11 0.08 0 0 0.09 0.07 0.1 0.12 0.13 0.12
Nucleus 0.07 0.1 0 0.1 0 0.07 0.08 0.09 0.11 0.09 0.08 0.17 0.08 0.09 0.09 0.14 0.23 0.11 0.16 0.09 0.07 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.13 0.27 0.12 0.08 0.05 0.06 0.06 0.07 0 0 0 0 0 0.05 0.11 0.13 0.16 0.05 0 0
Vac/Vac Membrane 0.24 0.29 0.1 0 0.08 0.08 0.05 0.05 0 0.06 0.08 0.19 0.24 0.19 0.39 0.43 0.34 0.21 0.28 0.21 0.36 0.34 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 3 0 0 0 0 1 0 0 0 0 0 0 0 1 3 2 1 5 7
Bud 0 0 2 6 4 5 6 11 7 8 11 6 1 0 0 0 0 1 2 3 5 6 11
Bud Neck 0 0 12 6 28 12 13 4 4 10 8 0 0 0 1 0 0 0 3 2 3 3 9
Bud Site 0 0 0 0 2 5 0 5 6 12 9 3 0 0 0 0 0
Cell Periphery 0 0 0 1 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 1 2
Cytoplasm 77 94 191 133 142 197 279 176 232 158 185 165 249 278 89 43 24 64 61 104 110 167 324
Endoplasmic Reticulum 3 1 0 0 3 0 1 0 0 0 1 2 2 1 9 6 3 2 1 2 4 5 5
Endosome 2 11 1 6 8 15 3 1 2 1 26 13 43 15 20 13 6 15 11 8 9 8 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 2
Mitochondria 40 34 0 30 18 98 141 247 257 213 150 110 45 50 15 2 1 19 16 32 42 60 76
Nucleus 10 19 7 20 10 24 37 38 58 34 34 70 38 41 17 16 14 22 39 27 27 16 11
Nuclear Periphery 0 0 0 0 0 0 0 2 0 0 1 0 0 2 0 0 0 2 1 0 0 0 0
Nucleolus 0 0 0 2 1 2 0 4 4 2 0 6 4 1 4 3 1 0 2 3 1 0 0
Peroxisomes 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 6 8 16 27 76 42 40 21 32 24 30 6 9 2 7 5 3 22 32 52 17 17 13
Vac/Vac Membrane 37 57 24 8 22 27 25 23 23 24 33 78 106 82 77 50 21 43 68 70 132 158 151
Unique Cell Count 152 195 238 204 278 359 478 428 520 388 414 407 449 436 197 115 61 207 250 328 364 463 638
Labelled Cell Count 175 224 255 241 317 428 545 532 626 487 490 459 497 472 241 138 73 207 250 328 364 463 638


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.8 4.8 4.1 4.8 3.8 3.9 3.4 3.4 3.2 3.9 3.8 3.8 4.0 5.8 6.0 5.9 3.7 3.8 4.0
Std Deviation (1e-4) 0.6 0.7 1.2 1.0 1.3 1.2 0.9 1.1 1.1 1.0 0.9 0.7 1.0 0.8 1.1 1.0 0.9 0.9 1.2 1.2
Intensity Change (Log2) -0.23 -0.0 -0.34 -0.31 -0.47 -0.48 -0.57 -0.29 -0.33 -0.31 -0.27 0.29 0.33 0.3 -0.36 -0.33 -0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 7.1 7.2 4.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slx4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available