Standard name
Human Ortholog
Description Endonuclease involved in processing DNA; acts during recombination, repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in complex with Slx1p; involved in interstrand cross-link repair, Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress; FANCP-related factor

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.51 0.48 0.8 0.65 0.51 0.55 0.58 0.41 0.45 0.41 0.45 0.41 0.55 0.64 0.45 0.37 0.39 0.31 0.25 0.32 0.3 0.36 0.51
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 0 0 0 0.06 0 0.1 0 0.1 0.11 0.1 0.08 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.17 0 0.15 0.06 0.27 0.29 0.58 0.49 0.55 0.36 0.27 0.1 0.11 0.08 0 0 0.09 0.07 0.1 0.12 0.13 0.12
Nucleus 0.07 0.1 0 0.1 0 0.07 0.08 0.09 0.11 0.09 0.08 0.17 0.08 0.09 0.09 0.14 0.23 0.11 0.16 0.09 0.07 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.13 0.27 0.12 0.08 0.05 0.06 0.06 0.07 0 0 0 0 0 0.05 0.11 0.13 0.16 0.05 0 0
Vac/Vac Membrane 0.24 0.29 0.1 0 0.08 0.08 0.05 0.05 0 0.06 0.08 0.19 0.24 0.19 0.39 0.43 0.34 0.21 0.28 0.21 0.36 0.34 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 3 0 0 0 0 1 0 0 0 0 0 0 0 1 3 2 1 5 7
Bud 0 0 2 6 4 5 6 11 7 8 11 6 1 0 0 0 0 1 2 3 5 6 11
Bud Neck 0 0 12 6 28 12 13 4 4 10 8 0 0 0 1 0 0 0 3 2 3 3 9
Bud Site 0 0 0 0 2 5 0 5 6 12 9 3 0 0 0 0 0
Cell Periphery 0 0 0 1 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 1 2
Cytoplasm 77 94 191 133 142 197 279 176 232 158 185 165 249 278 89 43 24 64 61 104 110 167 324
Endoplasmic Reticulum 3 1 0 0 3 0 1 0 0 0 1 2 2 1 9 6 3 2 1 2 4 5 5
Endosome 2 11 1 6 8 15 3 1 2 1 26 13 43 15 20 13 6 15 11 8 9 8 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 2
Mitochondria 40 34 0 30 18 98 141 247 257 213 150 110 45 50 15 2 1 19 16 32 42 60 76
Nucleus 10 19 7 20 10 24 37 38 58 34 34 70 38 41 17 16 14 22 39 27 27 16 11
Nuclear Periphery 0 0 0 0 0 0 0 2 0 0 1 0 0 2 0 0 0 2 1 0 0 0 0
Nucleolus 0 0 0 2 1 2 0 4 4 2 0 6 4 1 4 3 1 0 2 3 1 0 0
Peroxisomes 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 6 8 16 27 76 42 40 21 32 24 30 6 9 2 7 5 3 22 32 52 17 17 13
Vac/Vac Membrane 37 57 24 8 22 27 25 23 23 24 33 78 106 82 77 50 21 43 68 70 132 158 151
Unique Cell Count 152 195 238 204 278 359 478 428 520 388 414 407 449 436 197 115 61 207 250 328 364 463 638
Labelled Cell Count 175 224 255 241 317 428 545 532 626 487 490 459 497 472 241 138 73 207 250 328 364 463 638


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.8 4.8 4.1 4.8 3.8 3.9 3.4 3.4 3.2 3.9 3.8 3.8 4.0 5.8 6.0 5.9 3.7 3.8 4.0
Std Deviation (1e-4) 0.6 0.7 1.2 1.0 1.3 1.2 0.9 1.1 1.1 1.0 0.9 0.7 1.0 0.8 1.1 1.0 0.9 0.9 1.2 1.2
Intensity Change (Log2) -0.23 -0.0 -0.34 -0.31 -0.47 -0.48 -0.57 -0.29 -0.33 -0.31 -0.27 0.29 0.33 0.3 -0.36 -0.33 -0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 7.1 7.2 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5113 -0.9421 -0.3618 -0.0836 0.7351 -0.9331 -0.9205 -0.8137 -0.7188 -0.8825 -0.5728 -0.8295 -0.6988 -0.5866 -0.6879 -0.8559 -0.8383 -0.9902
Actin 0.0302 0.0005 0.0386 0.0137 0.0019 0.0007 0.0132 0.0007 0.0268 0.0056 0.0026 0.0037 0.0334 0.0012 0.0021 0.0221 0.0065 0.0002
Bud 0.0004 0.0001 0.0006 0.0004 0.0002 0.0001 0.0015 0.0006 0.0004 0.0002 0.0003 0.0008 0.0005 0.0017 0.0012 0.0003 0.0001 0.0001
Bud Neck 0.0065 0.0005 0.0017 0.0029 0.0005 0.0017 0.0041 0.0004 0.0011 0.001 0.0014 0.0035 0.0103 0.0002 0.0017 0.0004 0.0007 0.0013
Bud Periphery 0.0006 0.0002 0.0006 0.0009 0.0002 0.0002 0.0021 0.0009 0.0017 0.0003 0.0004 0.0008 0.0007 0.0004 0.0013 0.0007 0.0002 0
Bud Site 0.0036 0.0014 0.0109 0.0028 0.0002 0.0003 0.0175 0.005 0.0015 0.001 0.0007 0.0006 0.015 0.0043 0.0019 0.0003 0.0002 0.0001
Cell Periphery 0.0004 0.0002 0.0003 0.0007 0.0001 0 0.0008 0.0002 0.0001 0.0001 0.0001 0.0002 0.0007 0.0001 0.0002 0.0001 0 0
Cytoplasm 0.0885 0.1356 0.1981 0.063 0.048 0.1485 0.1656 0.1274 0.1059 0.1359 0.1053 0.211 0.2013 0.2138 0.2035 0.0972 0.1683 0.2828
Cytoplasmic Foci 0.063 0.0201 0.0307 0.0282 0.0367 0.0168 0.027 0.0152 0.032 0.023 0.0403 0.0109 0.0746 0.0219 0.0329 0.0034 0.0052 0.0057
Eisosomes 0.0004 0.0002 0.0004 0.001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0009 0.0002 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0097 0.0053 0.0142 0.0033 0.0032 0.0022 0.0057 0.0041 0.0042 0.0036 0.0024 0.0034 0.0067 0.0028 0.0078 0.0103 0.0052 0.0023
Endosome 0.0449 0.0042 0.0383 0.075 0.111 0.0036 0.0287 0.0093 0.0129 0.0083 0.0564 0.0062 0.029 0.0058 0.0214 0.0037 0.0377 0.0052
Golgi 0.0081 0.0002 0.0108 0.0298 0.0655 0.0008 0.0084 0.0003 0.0071 0.0007 0.0156 0.0003 0.0062 0.0011 0.0016 0.001 0.003 0.0002
Lipid Particles 0.0315 0.0108 0.0054 0.1126 0.0045 0.007 0.0185 0.0021 0.0204 0.0018 0.0251 0.0012 0.0147 0.0054 0.0031 0.001 0.003 0.0014
Mitochondria 0.0054 0.0005 0.0072 0.0257 0.0139 0.001 0.0069 0.0008 0.0134 0.0014 0.0102 0.0094 0.003 0.0005 0.002 0.0008 0.0021 0.0004
None 0.432 0.6713 0.3066 0.409 0.4799 0.6396 0.3496 0.615 0.354 0.3645 0.2581 0.3283 0.2809 0.5617 0.4158 0.5194 0.6313 0.346
Nuclear Periphery 0.0556 0.0064 0.0304 0.0073 0.1231 0.0075 0.0353 0.0177 0.0303 0.0205 0.0129 0.0129 0.0321 0.0063 0.0203 0.0089 0.0294 0.0176
Nucleolus 0.0038 0.0049 0.0074 0.0079 0.001 0.004 0.0068 0.0061 0.0071 0.0168 0.0057 0.0051 0.0036 0.0016 0.0167 0.0063 0.0008 0.0023
Nucleus 0.139 0.1001 0.2125 0.1789 0.069 0.1308 0.2266 0.1495 0.2366 0.3051 0.2884 0.3291 0.225 0.1263 0.1449 0.3021 0.0942 0.2621
Peroxisomes 0.0192 0.0004 0.002 0.008 0.0006 0.0066 0.0139 0.0004 0.0113 0.0038 0.048 0.0004 0.0102 0.0022 0.0012 0.0002 0.0006 0.0001
Punctate Nuclear 0.0499 0.0345 0.0775 0.0137 0.0305 0.0273 0.0545 0.0383 0.1296 0.1033 0.1199 0.069 0.0462 0.04 0.1077 0.02 0.0076 0.0701
Vacuole 0.0046 0.0024 0.0045 0.0113 0.0042 0.001 0.0098 0.005 0.0022 0.0025 0.0037 0.0025 0.0036 0.0021 0.0101 0.0012 0.0019 0.0016
Vacuole Periphery 0.0028 0.0003 0.001 0.0038 0.0059 0.0002 0.0032 0.001 0.001 0.0007 0.0024 0.0006 0.0014 0.0003 0.0025 0.0004 0.002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.3301 1.7559 1.303 4.1029 10.1868 8.8341 5.77 5.9913 4.0657 7.1794
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
209 805 455 1681 2033 1961 1742 1588 2242 2766 2197 3269

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 634.97 740.99 874.32 846.99 682.08 713.19 783.14 819.44 677.69 721.28 802.02 833.61
Standard Deviation 74.48 106.74 90.65 114.97 70.68 96.33 144.23 104.18 72.35 100.27 139.86 110.72
Intensity Change Log 2 0.222766 0.461473 0.415657 0.064346 0.199329 0.264697 0.142896 0.331664 0.339451

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000026 0.000411 0.000963 0.001354 0.000060 0.000259 0.000248 0.000386 0.000056 0.000303 0.000396 0.000883
Bud Neck 0.005599 0.015534 0.005321 0.027735 0.006213 0.013659 0.006109 0.014090 0.006155 0.014205 0.005946 0.021107
Bud Site 0.000358 0.009559 0.005415 0.038633 0.001153 0.005641 0.005503 0.015283 0.001079 0.006781 0.005485 0.027290
Cell Periphery 0.000152 0.000154 0.000108 0.000155 0.000128 0.000140 0.000380 0.000136 0.000131 0.000144 0.000324 0.000146
Cytoplasm 0.326448* 0.308035* 0.052985 0.173921* 0.358672* 0.290457* 0.191136* 0.133676* 0.355668* 0.295573* 0.162525* 0.154371*
Cytoplasmic Foci 0.191094* 0.125014 0.003525 0.006338 0.124237 0.137120 0.009184 0.003223 0.130469 0.133597 0.008012 0.004825
Eisosomes 0.000165 0.000091 0.000057 0.000037 0.000078 0.000088 0.000105 0.000027 0.000086 0.000089 0.000095 0.000032
Endoplasmic Reticulum 0.000376 0.002201 0.007663 0.005334 0.000959 0.001242 0.001990 0.004741 0.000905 0.001521 0.003165 0.005046
Endosome 0.005483 0.009880 0.001342 0.008345 0.004219 0.009924 0.001400 0.003549 0.004337 0.009911 0.001388 0.006015
Golgi 0.000291 0.002437 0.000084 0.005679 0.000620 0.003429 0.000133 0.000743 0.000589 0.003141 0.000123 0.003281
Lipid Particles 0.011454 0.008914 0.001605 0.001213 0.016176 0.017355 0.000988 0.001004 0.015736 0.014898 0.001116 0.001111
Mitochondria 0.000700 0.008644 0.001048 0.008398 0.006279 0.012177 0.001264 0.002278 0.005759 0.011148 0.001220 0.005425
Mitotic Spindle 0.000026 0.025282 0.004452 0.078323 0.001107 0.016092 0.007934 0.026235 0.001006 0.018766 0.007213 0.053020
None 0.007025 0.009657 0.004672 0.007586 0.005387 0.009088 0.049027 0.007230 0.005539 0.009254 0.039841 0.007413
Nuclear Periphery 0.000646 0.001898 0.001819 0.004702 0.001820 0.002014 0.001597 0.005873 0.001711 0.001980 0.001643 0.005271
Nuclear Periphery Foci 0.000979 0.004414 0.004878 0.006194 0.001708 0.002847 0.004159 0.003620 0.001640 0.003303 0.004308 0.004943
Nucleolus 0.008176 0.009386 0.006086 0.004338 0.007671 0.012333 0.004720 0.004013 0.007718 0.011476 0.005003 0.004181
Nucleus 0.426159* 0.410050* 0.877175* 0.569605* 0.445969* 0.435989* 0.700809* 0.752305* 0.444122* 0.428440* 0.737334* 0.658356*
Peroxisomes 0.004003 0.014531 0.000809 0.001414 0.005142 0.011524 0.000399 0.000768 0.005036 0.012399 0.000484 0.001100
Vacuole 0.010687 0.032139 0.019761 0.045055 0.012029 0.017321 0.012652 0.019738 0.011904 0.021634 0.014125 0.032757
Vacuole Periphery 0.000152 0.001767 0.000235 0.005642 0.000375 0.001303 0.000262 0.001081 0.000354 0.001438 0.000256 0.003427

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.78 -3.51 -8.54 -4.00 1.05 -4.25 -9.82 -15.71 -8.28 -5.49 -7.51 -6.58 -13.12 -7.89 -2.17
Bud Neck -3.31 -2.16 -6.24 -4.04 -6.83 -6.47 -4.12 -9.06 -4.38 -6.08 -7.64 -4.82 -13.28 -6.66 -9.45
Bud Site -5.15 -4.31 -9.74 -4.07 -4.55 -4.87 -5.54 -8.09 -4.56 -4.47 -6.76 -6.77 -12.27 -6.85 -7.25
Cell Periphery -0.08 0.88 1.51 2.30 0.72 -0.57 -4.12 -0.24 0.71 4.58 -0.79 -3.94 -0.13 1.75 4.67
Cytoplasm 1.20 15.60 11.17 16.20 -12.00 8.75 25.49 34.07 24.45 6.70 8.76 31.07 37.17 29.33 2.68
Cytoplasmic Foci 5.12 14.55 14.57 20.37 0.22 -2.27 30.83 32.58 33.23 6.01 -0.17 33.94 35.33 38.89 5.75
Eisosomes 2.59 2.95 4.09 7.14 3.19 -2.34 -0.69 7.69 9.69 2.21 -0.84 -0.21 8.41 11.92 2.42
Endoplasmic Reticulum -9.90 -13.45 -26.75 -16.59 3.04 -2.03 -15.12 -20.83 -19.90 -8.61 -5.95 -19.35 -31.70 -28.06 -7.08
Endosome -2.08 1.83 1.16 6.61 -2.75 -6.75 6.32 3.13 8.85 -2.65 -7.91 6.39 2.85 10.73 -4.42
Golgi -3.81 2.53 -1.70 1.18 -2.04 -5.18 5.67 1.57 5.54 -1.63 -6.04 5.92 -0.85 4.29 -2.35
Lipid Particles 1.08 4.82 5.17 10.72 2.49 -0.68 13.20 13.18 14.27 -0.18 0.56 13.77 13.86 16.55 0.57
Mitochondria -5.45 -2.52 -6.54 2.64 -5.38 -3.87 4.64 3.62 8.81 -4.36 -4.06 4.61 2.39 8.30 -6.36
Mitotic Spindle -7.54 -4.03 -16.31 -7.36 -9.87 -8.27 -7.10 -11.86 -6.79 -6.90 -10.97 -8.04 -19.84 -11.41 -12.72
None -1.43 2.41 0.21 2.09 -3.68 -4.68 -8.30 -2.87 2.36 7.63 -5.03 -7.92 -2.61 3.43 7.37
Nuclear Periphery -7.19 -10.82 -15.64 -12.34 -11.50 -0.81 -7.19 -10.23 -9.89 -6.34 -1.94 -8.15 -16.63 -15.82 -10.97
Nuclear Periphery Foci -3.32 -3.25 -4.84 -0.64 1.38 -2.09 -4.56 -6.11 -4.93 -1.99 -3.66 -5.53 -7.84 -4.74 -0.62
Nucleolus -0.22 0.17 0.57 1.47 0.55 -3.19 -1.06 -0.49 2.57 0.57 -2.81 -0.87 0.39 3.52 1.31
Nucleus 0.65 -17.87 -8.92 -15.03 15.38 2.08 -28.44 -25.91 -26.91 1.37 2.76 -34.20 -25.80 -29.12 10.34
Peroxisomes -4.87 2.75 3.20 9.73 1.10 -7.74 10.42 10.46 15.62 0.39 -9.29 10.55 10.86 18.27 0.80
Vacuole -7.97 -5.30 -17.85 -10.27 -8.88 -4.19 -7.37 -11.75 -9.76 -6.04 -8.98 -9.07 -22.20 -16.64 -13.39
Vacuole Periphery -3.26 -2.66 -3.44 0.01 -3.08 -3.89 2.17 -0.90 2.49 -1.63 -4.84 1.93 -3.11 0.88 -3.55