Standard name
Description mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; TIS11 has a paralog, CTH1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.93 0.98 0.98 0.97 0.95 0.9 0.91 0.78 0.84 0.79 0.8 0.96 0.94 0.96 0.95 0.89 0.81 0.82 0.8 0.62 0.56 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0.06 0.1 0.15 0.07 0.39 0.24 0.31 0.3 0 0 0 0.07 0 0.09 0.05 0.06 0.18 0.24 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.08 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 2 1 0 0 0 0 1 0 0 0 1 1 5 1 3 3
Bud 0 1 0 1 0 1 6 3 8 2 7 6 0 1 0 0 0 0 0 11 3 4 9
Bud Neck 0 1 13 4 7 2 9 16 2 5 6 8 0 0 0 0 0 0 1 2 0 0 2
Bud Site 0 0 0 0 0 0 0 0 1 3 0 4 0 0 0 0 0
Cell Periphery 2 0 4 2 2 1 2 1 2 1 3 1 0 0 0 1 0 0 0 2 0 1 1
Cytoplasm 146 198 484 365 352 464 446 642 405 427 436 474 131 207 227 95 117 138 180 387 136 131 144
Endoplasmic Reticulum 5 4 0 0 0 0 1 0 1 0 0 0 0 2 0 9 12 0 0 2 3 3 1
Endosome 1 0 0 0 1 2 0 2 2 0 3 2 0 0 0 1 5 3 5 5 5 5 7
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 2 4 7 6
Mitochondria 7 13 0 10 22 48 75 49 201 122 170 181 0 0 0 7 4 14 10 28 40 55 39
Nucleus 0 0 2 1 3 1 8 7 6 4 3 2 2 0 1 2 0 0 0 1 1 1 0
Nuclear Periphery 0 2 0 0 0 0 1 0 1 2 0 2 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 0 1 2 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 2 0 0 0 1 1 0 0 0 0 0 0 0 1 0 3 3 7
SpindlePole 0 0 2 1 1 3 1 11 5 6 6 13 0 1 0 0 0 1 2 10 6 5 4
Vac/Vac Membrane 2 3 3 6 1 6 0 6 5 6 7 6 9 18 19 0 11 3 2 5 2 4 2
Unique Cell Count 159 212 492 372 362 490 497 703 517 509 549 596 137 220 237 100 132 171 219 482 221 236 237
Labelled Cell Count 163 222 509 391 390 530 550 741 640 580 642 699 142 230 247 117 149 171 219 482 221 236 237


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.2 5.3 5.0 4.2 3.9 3.2 4.4 3.2 3.7 3.6 3.8 16.5 14.5 13.4 4.5 5.9 6.0 6.0 6.2 6.4
Std Deviation (1e-4) 0.6 1.4 1.9 2.1 1.4 1.1 1.2 1.6 1.0 1.1 1.0 1.0 4.5 3.8 3.1 2.3 2.6 2.5 2.4 1.9 2.2
Intensity Change (Log2) -0.07 -0.32 -0.43 -0.73 -0.26 -0.72 -0.5 -0.53 -0.48 1.65 1.46 1.35 -0.22 0.16 0.19 0.18 0.24 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tis11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available