Standard name
Description Mitochondrial membrane protein; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0.29 0.07 – – 0 0 0 0 0 0 0
Bud 0 0.06 0 0 0 0 0 0.05 0.05 0 0.05 0.05 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 – – 0.05 0 0 0 0 0 0
Cytoplasm 0 0.08 0 0 0 0.07 0 0 0 0 0.06 0 0.12 0.19 0.38 – – 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 – – 0.12 0 0 0 0 0 0
Endosome 0 0 0.06 0 0.12 0 0 0 0 0 0 0 0 0 0.06 – – 0.26 0.08 0.05 0.08 0.1 0.08 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.91 0.92 0.88 0.87 0.84 0.9 0.9 0.87 0.93 0.96 0.94 0.96 0.44 0.2 0.2 – – 0.11 0.76 0.79 0.7 0.7 0.66 0.68
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.09 0.14 0 0 0 0 0 0 0 0 0 0 – – 0.18 0.09 0 0.12 0.09 0.1 0.07
SpindlePole 0.08 0 0 0 0.07 0.05 0.08 0.08 0.05 0.05 0 0.06 0.17 0.18 0.09 – – 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.05 0 0 0.08 0.07 0 0 0.05 0 0 0 0.1 0.09 0.17 – – 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 12 0 0 0 0 0 0 0 0 0 0 15 63 18 – – 0 1 5 6 2 14 24
Bud 1 17 13 10 9 9 22 45 27 17 24 26 3 5 6 – – 1 0 0 0 1 3 1
Bud Neck 0 3 0 0 0 0 0 1 1 0 0 0 3 2 1 – – 0 0 0 3 4 4 14
Bud Site 2 2 0 0 0 9 14 19 10 12 10 9 0 0 1 – – 0 – – – – – –
Cell Periphery 2 6 5 10 4 1 2 3 1 3 1 5 8 10 9 – – 3 0 0 1 0 1 0
Cytoplasm 3 22 14 11 14 27 30 41 23 10 29 12 19 41 94 – – 13 0 2 1 1 3 1
Endoplasmic Reticulum 2 7 2 1 1 0 0 1 2 5 1 0 8 24 3 – – 8 0 1 2 2 1 6
Endosome 2 0 20 7 43 9 20 18 2 2 2 5 7 5 15 – – 17 10 12 29 37 51 51
Golgi 2 0 1 2 0 0 0 0 0 0 0 0 10 6 3 – – 0 3 2 6 12 25 16
Mitochondria 87 243 287 215 290 352 632 797 521 607 448 544 69 45 50 – – 7 98 215 241 267 417 446
Nucleus 0 1 0 0 2 10 25 34 6 7 10 4 4 5 3 – – 1 0 0 0 0 0 2
Nuclear Periphery 1 2 4 4 0 12 11 12 11 16 11 8 3 0 0 – – 0 0 2 3 1 5 5
Nucleolus 0 0 2 1 3 9 12 21 11 10 9 6 0 0 0 – – 0 0 0 1 3 3 3
Peroxisomes 2 0 35 22 49 12 30 36 8 10 5 10 3 3 10 – – 12 11 8 40 34 66 43
SpindlePole 8 4 9 8 24 20 59 73 26 29 18 34 26 40 22 – – 7 0 2 0 2 13 5
Vac/Vac Membrane 8 14 9 8 29 26 30 35 28 18 9 15 16 20 41 – – 10 0 9 2 4 10 25
Unique Cell Count 96 263 327 246 347 391 702 916 559 633 477 569 157 220 245 66 129 271 347 382 633 657
Labelled Cell Count 121 333 401 299 468 496 887 1136 677 746 577 678 194 269 276 79 129 271 347 382 633 657


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 3.8 4.4 4.6 4.4 3.9 3.5 3.8 3.5 3.2 3.5 3.5 6.8 6.6 6.7 3.6 4.5 5.2 6.5 6.8 6.9
Std Deviation (1e-4) 0.6 0.5 1.2 1.4 1.2 1.6 1.2 1.5 1.4 0.8 1.4 1.1 0.9 0.9 1.0 0.5 1.1 1.3 1.5 1.5 1.8
Intensity Change (Log2) – – – 0.05 0.01 -0.2 -0.33 -0.23 -0.32 -0.49 -0.33 -0.35 0.62 0.58 0.61 -0.31 0.02 0.23 0.56 0.62 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5342 2.1214 2.4165 2.1377 1.3611 2.1663 1.5278 1.524 1.2787 1.5081 0.8222 1.247 0.8656 1.1296 0.7832 0.8846 0.3764 0.7834
Actin 0.0313 0.0081 0.0196 0.0212 0.0143 0.0259 0.0917 0.005 0.0103 0.0123 0.0009 0.0057 0.0139 0.0168 0.0226 0.0329 0.0027 0.0116
Bud 0.0006 0.0009 0.0003 0.0002 0.0045 0.0005 0.0008 0.0004 0.0006 0.0009 0.0001 0.0001 0.0004 0.0008 0.0004 0.0005 0.0005 0.0003
Bud Neck 0.0028 0.0004 0.0005 0.0003 0.0004 0.0011 0.0008 0.0002 0.0012 0.0004 0.0001 0.0005 0.0004 0.0004 0.001 0.0008 0.0003 0.0019
Bud Periphery 0.0009 0.0013 0.0004 0.0007 0.0038 0.0015 0.0014 0.0008 0.001 0.0015 0.0004 0.0004 0.0009 0.001 0.0006 0.0007 0.0013 0.0007
Bud Site 0.0052 0.0076 0.0009 0.0003 0.002 0.0018 0.0033 0.0011 0.0089 0.0009 0.0002 0.0002 0.0011 0.0057 0.0049 0.0011 0.0006 0.0003
Cell Periphery 0.0004 0.0006 0.0002 0.0004 0.0003 0.0005 0.0004 0.0002 0.0003 0.0002 0.0003 0.0001 0.0003 0.0002 0.0015 0.0002 0.0002 0.0002
Cytoplasm 0.0132 0.0043 0.0023 0.0002 0.0008 0.0009 0.0011 0.0006 0.0009 0.0002 0.0003 0.0002 0.0024 0.0023 0.0017 0.001 0.0028 0.0004
Cytoplasmic Foci 0.0219 0.0201 0.0107 0.0088 0.0336 0.0182 0.0222 0.0199 0.0118 0.0052 0.0118 0.008 0.025 0.0285 0.0261 0.0337 0.0395 0.0073
Eisosomes 0.0004 0.0004 0.0003 0.0036 0.0003 0.0003 0.0011 0.0002 0.0016 0.0003 0.0002 0.0002 0.0005 0.0003 0.0012 0.0004 0.0001 0.0003
Endoplasmic Reticulum 0.0104 0.0014 0.0014 0.0015 0.0003 0.0007 0.0009 0.0002 0.0002 0 0.0002 0.0001 0.0016 0.0006 0.0005 0.0006 0.0002 0.0007
Endosome 0.0881 0.0681 0.045 0.0219 0.0184 0.0377 0.0497 0.0349 0.0282 0.0039 0.0477 0.0217 0.0669 0.0569 0.0344 0.0333 0.0355 0.0331
Golgi 0.0716 0.0606 0.0368 0.0313 0.0549 0.0584 0.0526 0.052 0.0249 0.0165 0.0537 0.0438 0.0807 0.0733 0.067 0.0689 0.0389 0.0676
Lipid Particles 0.0373 0.0197 0.0061 0.037 0.0293 0.0313 0.0412 0.0185 0.0074 0.0004 0.0276 0.0337 0.0659 0.0157 0.0133 0.025 0.0079 0.0156
Mitochondria 0.6275 0.7119 0.8422 0.8515 0.7822 0.7634 0.5973 0.7616 0.8426 0.9506 0.7849 0.8394 0.6565 0.7532 0.7369 0.7107 0.8107 0.8068
None 0.0032 0.0011 0.0003 0.0006 0.0023 0.0085 0.0019 0.0004 0.0113 0.0001 0.0002 0.0001 0.0084 0.0003 0.0029 0.0006 0.0014 0.0001
Nuclear Periphery 0.009 0.0004 0.0011 0.0011 0.0004 0.001 0.0108 0.0001 0.0001 0 0.0003 0.0001 0.0072 0.0002 0.0022 0.0012 0.0001 0.0017
Nucleolus 0.0008 0.0002 0.0002 0.0001 0.001 0.0003 0.0005 0.0002 0.0003 0 0.0002 0.0001 0.0008 0.0001 0.0004 0.0002 0.0001 0.0001
Nucleus 0.0033 0.0003 0.0003 0.0002 0.0004 0.0003 0.0014 0.0001 0.0002 0 0.0001 0.0001 0.001 0.0001 0.0018 0.0002 0.0001 0.0002
Peroxisomes 0.056 0.0831 0.0222 0.0137 0.0465 0.0421 0.11 0.0995 0.0452 0.0056 0.0651 0.0426 0.0523 0.0408 0.0769 0.0792 0.0526 0.0449
Punctate Nuclear 0.0058 0.0003 0.0002 0.0002 0.0019 0.0003 0.0029 0.0001 0.0002 0 0.0001 0.0001 0.0042 0.0001 0.0008 0.0065 0.0002 0.0001
Vacuole 0.005 0.0046 0.006 0.0022 0.001 0.0026 0.0035 0.0017 0.0015 0.0003 0.0024 0.0008 0.0042 0.0012 0.0014 0.0009 0.0014 0.0026
Vacuole Periphery 0.0052 0.0046 0.0029 0.0029 0.0014 0.0025 0.0044 0.0021 0.0011 0.0005 0.0031 0.002 0.0054 0.0017 0.0016 0.0014 0.0028 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.569 9.1898 4.925 8.851 6.2941 6.8496 8.7756 10.2781 7.3656 9.7771
Translational Efficiency 1.0083 0.5914 1.1952 0.5402 0.6854 0.6786 0.6032 0.5915 0.8825 0.6868

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘