Standard name
Human Ortholog
Description Component of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.61 0.22 0.75 0.61 0.62 0.35 0.33 0.39 0.25 0.25 0.17 0.39 0.63 0.78 0.78 0.55 0.59 0.56 0.81 0.75 0.86 0.83 0.83 0.83
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Mitochondria 0.27 0.49 0.23 0.43 0.28 0.53 0.54 0.35 0.66 0.63 0.72 0.52 0.14 0 0 0.16 0.12 0.07 0 0.06 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.07 0 0.05 0 0 0 0 0 0
Nucleolus 0.43 0.29 0.23 0.23 0.17 0.14 0.13 0.14 0.1 0.11 0.11 0.08 0.37 0.24 0.25 0.2 0.2 0.26 0 0 0 0 0 0
Peroxisomes 0 0 0.16 0.07 0 0.07 0.1 0.12 0.07 0.07 0.05 0.13 0.08 0.08 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 0 0.22 0.17 0.15 0.12 0.15 0.21 0.08 0.1 0.08 0.12 0.22 0.26 0.28 0.13 0.14 0.13 0 0 0 0.06 0 0
Vac/Vac Membrane 0.29 0.32 0.1 0.09 0.15 0.1 0.08 0.09 0.07 0.09 0.09 0.13 0.16 0.3 0.19 0.41 0.38 0.41 0.05 0.09 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 3 4 2 2 2 3 0 0 1 2 0 1 0 0 1 0 1 2
Bud 0 0 12 6 6 24 35 50 28 44 36 35 2 1 0 2 0 2 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 0 2 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 2 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 23 15 34 33 14 29 18 9 8 16 4 6 6 6 11 7 0 0 0 0 0 1
Endoplasmic Reticulum 0 2 0 0 1 2 2 4 7 7 3 1 0 1 2 1 1 7 0 0 2 0 1 3
Endosome 120 34 524 316 468 300 322 388 192 230 116 414 74 209 207 69 90 78 170 130 624 133 260 305
Golgi 1 2 2 2 3 3 1 6 2 7 0 5 0 2 4 7 8 7 5 2 17 1 3 6
Mitochondria 53 76 164 222 212 453 534 350 502 582 495 558 16 5 7 20 18 10 4 10 6 2 9 9
Nucleus 0 4 0 0 7 2 13 7 2 4 1 4 2 1 1 1 1 1 0 1 0 0 2 1
Nuclear Periphery 9 16 14 11 5 12 24 16 17 15 8 15 7 5 7 9 2 7 3 1 10 0 1 3
Nucleolus 85 44 161 119 130 122 124 142 79 100 74 84 44 65 65 25 30 36 1 1 1 1 1 0
Peroxisomes 2 0 111 38 21 61 97 117 55 65 36 135 10 21 11 1 2 0 4 1 16 2 6 5
SpindlePole 19 4 153 88 117 99 151 210 59 94 56 133 26 69 74 16 21 18 8 4 27 9 9 4
Vac/Vac Membrane 57 49 72 44 112 89 79 90 52 81 62 138 19 81 51 52 57 58 9 16 6 6 12 20
Unique Cell Count 198 154 700 517 756 848 989 1007 755 917 690 1074 118 269 264 126 152 140 212 173 723 161 314 367
Labelled Cell Count 348 237 1236 861 1116 1201 1400 1419 1015 1242 900 1546 204 466 436 211 241 232 212 173 723 161 314 367


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.7 7.1 6.8 6.4 5.3 5.4 6.2 5.8 6.0 5.8 6.0 8.3 8.0 8.1 9.2 8.7 9.1 7.0 6.9 7.4
Std Deviation (1e-4) 0.9 1.2 1.2 1.4 1.5 1.5 1.0 1.4 1.3 1.1 1.1 1.1 9.2 0.9 1.1 1.9 1.7 1.7 0.9 1.1 1.2
Intensity Change (Log2) – – – -0.07 -0.14 -0.42 -0.38 -0.2 -0.28 -0.25 -0.29 -0.24 0.23 0.17 0.19 0.38 0.3 0.36 -0.02 -0.04 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.8 -1.6 1.4 2.2 3.5 2.3 3.4 3.6 2.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 1.2 0.6 -2.6 -0.5 -1.0 -3.3 -2.7 -2.5 0 -0.9 -0.8 0 2.3 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -5.1 -5.3 -15.5 -17.1 -14.8 -18.8 -19.9 -21.7 -15.0 -2.8 0.9 1.1 -4.6 -3.9 -4.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 7.2 2.0 12.0 12.6 5.0 16.5 16.0 18.0 12.0 -2.4 -7.9 -7.5 -1.9 -3.2 -4.3
Nucleus 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.2 -2.2 -0.9 0.6 -0.6 0.3 -0.5 -1.3 -1.0 0 -0.1 0.6 0 0 0
Nucleolus 0 -2.8 -4.4 -5.7 -4.7 -6.4 -6.5 -6.1 -9.1 3.3 0.4 0.5 -0.8 -0.9 0.7
Peroxisomes -4.5 -8.7 -5.4 -3.7 -2.5 -5.1 -5.6 -6.4 -2.0 -2.1 -3.3 -4.9 -4.5 -4.8 -5.1
SpindlePole -2.1 -3.1 -5.4 -3.5 -0.5 -7.6 -6.4 -7.2 -5.3 0 1.3 2.0 -2.3 -2.2 -2.4
Vacuole -1.0 2.6 0.1 -1.6 -0.9 -2.3 -1.0 -0.8 1.6 1.9 7.6 3.7 9.0 8.5 9.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0628 2.5346 2.341 1.7931 1.8385 2.1899 3.5594 4.1402 3.9164 3.8148 3.8673 4.0629 2.12 2.9474 2.725 2.6916 2.7473 2.4627
Actin 0.0001 0.0004 0.0002 0 0.0002 0.0001 0.0025 0 0.0027 0.005 0.0006 0.0016 0.0131 0.0001 0.0016 0.005 0.0009 0.0006
Bud 0 0.0002 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0003 0 0.0002 0.0005 0.0002 0
Bud Neck 0.0002 0.0003 0.0003 0.0002 0.0003 0.0003 0.0007 0.0006 0.0004 0.0004 0.0004 0.0003 0.0013 0.001 0.0003 0.0008 0.0003 0.0002
Bud Periphery 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0003 0.0001 0.0002 0.0006 0.0002 0.0001
Bud Site 0.0003 0.0005 0.0002 0.0001 0.0003 0.0001 0.0004 0.0005 0.0003 0.0011 0.0003 0.0002 0.0067 0.0003 0.0007 0.0019 0.0003 0.0001
Cell Periphery 0.0001 0.0002 0.0001 0 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0007 0.0008 0.0006 0.0006 0.0005 0.0004 0.0059 0.0004 0.0005 0.0013 0.0008 0.0021 0.0055 0.0005 0.0013 0.0007 0.0005 0.0003
Cytoplasmic Foci 0.1339 0.1346 0.1343 0.1854 0.1526 0.0822 0.1204 0.1528 0.1369 0.1445 0.1249 0.0983 0.1363 0.134 0.1351 0.1475 0.1286 0.073
Eisosomes 0 0 0 0 0 0 0 0 0.0002 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0003 0.0002 0.0002 0.0002 0.0001 0.0002 0.0008 0.0001 0.0002 0.0016 0.001 0.0003 0.0016 0.0001 0.0002 0.0002 0.0002 0.0002
Endosome 0.4171 0.3472 0.4032 0.5178 0.4997 0.4102 0.4014 0.3353 0.3739 0.5082 0.4604 0.3816 0.3916 0.3429 0.3973 0.5926 0.5155 0.2951
Golgi 0.056 0.0721 0.0972 0.1236 0.0957 0.095 0.0709 0.0715 0.0893 0.0808 0.0913 0.0901 0.0491 0.0638 0.0907 0.0798 0.116 0.1032
Lipid Particles 0.2997 0.3392 0.2583 0.07 0.1959 0.3138 0.3028 0.3308 0.2486 0.1701 0.2462 0.3444 0.2729 0.3597 0.2464 0.089 0.1461 0.4477
Mitochondria 0.0024 0.0096 0.0133 0.0011 0.0029 0.0072 0.0063 0.0016 0.0148 0.0019 0.0113 0.0034 0.022 0.0174 0.0101 0.0025 0.035 0.0064
None 0.0006 0.0001 0.0001 0 0.0001 0.0001 0.0018 0.0001 0.0004 0.0005 0.0002 0.0002 0.0038 0.0001 0.0014 0.0002 0.0001 0.0001
Nuclear Periphery 0.0051 0.0005 0.0007 0.0005 0.0001 0.0005 0.0009 0.0002 0.0002 0.0013 0.002 0.0007 0.0014 0.0004 0.0003 0.0002 0.0001 0.0003
Nucleolus 0.0048 0.0031 0.0021 0.0003 0.0023 0.0037 0.0009 0.0087 0.0017 0.0002 0.0031 0.0005 0.0034 0.0052 0.0008 0.0006 0.0002 0.0038
Nucleus 0.0013 0.0008 0.0003 0.0001 0.0002 0.0005 0.0007 0.0007 0.0002 0.0002 0.0005 0.003 0.0009 0.0003 0.0002 0.0002 0.0001 0.0003
Peroxisomes 0.0442 0.0393 0.0484 0.0491 0.0347 0.0478 0.0599 0.0711 0.1111 0.0706 0.0361 0.054 0.0482 0.0334 0.069 0.0518 0.0448 0.0489
Punctate Nuclear 0.0034 0.0042 0.0007 0.0005 0.0035 0.0005 0.0046 0.0079 0.0008 0.0023 0.0019 0.0043 0.0082 0.0007 0.0008 0.0012 0.0004 0.0023
Vacuole 0.0228 0.035 0.0286 0.0316 0.0076 0.0238 0.0131 0.0143 0.0137 0.0073 0.0117 0.0084 0.0238 0.0223 0.0329 0.0174 0.0072 0.01
Vacuole Periphery 0.007 0.0116 0.011 0.0188 0.0033 0.0134 0.0058 0.0029 0.0039 0.0025 0.007 0.0065 0.0094 0.0176 0.0102 0.0071 0.0031 0.0075

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.2078 10.3754 9.5573 16.8275 18.421 11.1999 14.4697 15.2595 15.5483 16.937
Translational Efficiency 0.8351 1.0686 1.261 0.6825 0.5729 1.0871 0.9331 0.7442 0.9051 0.7598

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1213 1893 269 241 2183 1901 2097 119 3396 3794 2366 360

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 794.64 880.03 996.80 1040.58 839.04 803.99 924.63 1210.39 823.18 841.93 932.84 1096.71
Standard Deviation 98.85 114.54 122.89 157.26 108.67 118.12 122.98 209.19 107.40 122.40 125.08 193.40
Intensity Change Log 2 — 0.147251 0.327003 0.389015 — -0.061562 0.140137 0.528660 — 0.043784 0.234055 0.462421

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000222 0.000115 0.000267 0.000607 0.000116 0.000114 0.000060 0.000130 0.000153 0.000114 0.000083 0.000450
Bud Neck 0.001751 0.001399 0.024040 0.007559 0.001112 0.001255 0.009225 0.003664 0.001341 0.001327 0.010910 0.006271
Bud Site 0.002049 0.001395 0.016097 0.050267 0.000998 0.001426 0.007124 0.012945 0.001374 0.001410 0.008144 0.037930
Cell Periphery 0.000161 0.000058 0.000211 0.000260 0.000088 0.000056 0.000128 0.000131 0.000114 0.000057 0.000137 0.000217
Cytoplasm 0.001694 0.000521 0.002936 0.013354 0.000560 0.001233 0.002398 0.002354 0.000965 0.000878 0.002459 0.009718
Cytoplasmic Foci 0.062173 0.064520 0.082246 0.105012 0.080440 0.066687 0.045329 0.031488 0.073915 0.065606 0.049526 0.080708
Eisosomes 0.000027 0.000015 0.000068 0.000019 0.000016 0.000017 0.000035 0.000010 0.000020 0.000016 0.000038 0.000016
Endoplasmic Reticulum 0.000468 0.000162 0.000927 0.000571 0.000145 0.000210 0.000584 0.000242 0.000261 0.000186 0.000623 0.000462
Endosome 0.732304* 0.762744* 0.523821* 0.436322* 0.716922* 0.729522* 0.610482* 0.196679 0.722416* 0.746098* 0.600629* 0.357107*
Golgi 0.062194 0.055909 0.022054 0.074859 0.096213 0.082762 0.034172 0.107587 0.084062 0.069364 0.032795 0.085678
Lipid Particles 0.022292 0.020379 0.016413 0.005881 0.016225 0.016239 0.005398 0.000604 0.018392 0.018305 0.006651 0.004136
Mitochondria 0.031716 0.016071 0.010025 0.005963 0.027963 0.024288 0.014960 0.008235 0.029303 0.020188 0.014399 0.006714
Mitotic Spindle 0.001154 0.002260 0.019336 0.017578 0.001064 0.003102 0.010822 0.006853 0.001096 0.002682 0.011790 0.014033
None 0.000162 0.000045 0.000260 0.000328 0.000041 0.000155 0.000879 0.000098 0.000084 0.000100 0.000809 0.000252
Nuclear Periphery 0.000247 0.000305 0.002859 0.000928 0.000166 0.000258 0.001326 0.001091 0.000195 0.000281 0.001500 0.000982
Nuclear Periphery Foci 0.003539 0.004784 0.024285 0.004129 0.001405 0.003779 0.008255 0.002317 0.002168 0.004280 0.010078 0.003530
Nucleolus 0.002768 0.000695 0.009308 0.000802 0.000488 0.000822 0.010278 0.000174 0.001302 0.000758 0.010168 0.000595
Nucleus 0.000859 0.000296 0.006600 0.001120 0.000167 0.000510 0.007537 0.000151 0.000414 0.000403 0.007431 0.000800
Peroxisomes 0.020035 0.010898 0.053658 0.019573 0.015153 0.013312 0.011295 0.002948 0.016897 0.012107 0.016112 0.014078
Vacuole 0.037296 0.040991 0.148978 0.205051* 0.026190 0.035643 0.171474 0.419756* 0.030157 0.038312 0.168917 0.276023*
Vacuole Periphery 0.016891 0.016439 0.035611 0.049816 0.014526 0.018610 0.048240 0.202543* 0.015371 0.017526 0.046804 0.100301*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.99 -0.69 -2.20 -2.76 -1.92 0.04 1.84 -0.38 -0.40 -1.51 1.17 2.73 -2.50 -2.79 -3.07
Bud Neck 0.68 -7.22 -6.56 -7.02 4.79 -0.42 -9.52 -1.67 -1.59 3.29 0.03 -11.51 -6.61 -6.67 3.36
Bud Site 1.17 -5.44 -6.75 -6.84 -4.69 -1.65 -8.32 -3.01 -2.91 -1.44 -0.19 -9.28 -7.21 -7.20 -5.94
Cell Periphery 2.67 -0.99 -1.56 -2.89 -1.04 1.47 -1.57 -1.60 -4.22 -0.15 2.91 -1.01 -2.21 -3.29 -1.88
Cytoplasm 1.64 -0.94 -3.86 -4.19 -3.42 -1.77 -5.70 -2.07 -1.26 0.06 0.21 -4.00 -4.20 -4.23 -3.59
Cytoplasmic Foci -0.69 -2.72 -5.15 -4.94 -2.33 4.02 11.37 7.03 5.10 2.05 3.30 9.41 -1.24 -2.55 -5.04
Eisosomes 6.48 -5.23 2.81 -2.28 6.05 -0.89 -5.49 2.49 3.17 6.48 3.16 -5.93 1.76 -0.40 6.49
Endoplasmic Reticulum 2.29 -2.23 -0.26 -1.18 0.99 -1.54 -6.83 -1.77 -0.62 4.66 1.36 -4.93 -0.85 -1.20 0.73
Endosome -3.50 11.67 16.44 18.55 4.06 -1.54 13.18 24.49 24.95 19.21* -4.03 17.17 24.53 26.21 15.91
Golgi 1.89 13.12 -1.27 -2.17 -6.94 3.45 20.45 -0.68 -1.51 -4.54 5.52 22.03 0.08 -1.93 -7.03
Lipid Particles 0.65 1.42 5.32 5.33 2.60 -0.03 6.46 9.98 8.96 7.13 0.04 7.94 7.60 7.66 1.71
Mitochondria 5.53 7.16 9.11 5.44 1.78 1.33 5.65 5.24 4.31 1.97 4.69 7.92 10.90 7.49 4.39
Mitotic Spindle -2.40 -3.28 -4.20 -3.88 0.21 -3.02 -7.49 -2.17 -1.40 1.33 -3.96 -8.11 -4.66 -4.07 -0.84
None 2.95 -0.80 -1.05 -1.59 -0.56 -1.65 -2.41 -1.29 0.74 2.24 -0.41 -2.35 -1.42 -1.29 1.48
Nuclear Periphery -1.16 -3.05 -2.55 -2.36 2.08 -1.76 -7.44 -1.36 -1.23 0.37 -2.38 -7.76 -2.80 -2.50 1.48
Nuclear Periphery Foci -1.25 -4.37 -0.33 0.49 4.16 -3.27 -7.27 -0.89 1.25 4.36 -3.66 -7.80 -1.30 0.79 4.90
Nucleolus 2.01 -3.15 1.76 -0.79 4.44 -1.29 -8.28 1.94 2.97 8.62 1.31 -7.93 1.49 0.26 8.86
Nucleus 2.16 -2.86 -1.27 -1.67 1.57 -1.83 -7.83 0.31 1.93 7.85 0.07 -8.08 -1.50 -1.51 4.29
Peroxisomes 5.41 -5.68 0.28 -2.71 5.37 1.08 2.57 8.15 7.52 7.09 4.07 0.54 1.41 -0.75 1.06
Vacuole -1.19 -10.14 -12.14 -11.95 -3.38 -4.43 -32.79 -16.81 -16.39 -10.43 -4.61 -33.60 -18.78 -18.15 -7.91
Vacuole Periphery 0.26 -4.15 -6.07 -6.25 -1.99 -2.60 -16.80 -11.54 -11.27 -9.43 -1.87 -17.26 -11.31 -11.01 -6.96