Standard name
Description Component of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.61 0.22 0.75 0.61 0.62 0.35 0.33 0.39 0.25 0.25 0.17 0.39 0.63 0.78 0.78 0.55 0.59 0.56 0.81 0.75 0.86 0.83 0.83 0.83
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Mitochondria 0.27 0.49 0.23 0.43 0.28 0.53 0.54 0.35 0.66 0.63 0.72 0.52 0.14 0 0 0.16 0.12 0.07 0 0.06 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.07 0 0.05 0 0 0 0 0 0
Nucleolus 0.43 0.29 0.23 0.23 0.17 0.14 0.13 0.14 0.1 0.11 0.11 0.08 0.37 0.24 0.25 0.2 0.2 0.26 0 0 0 0 0 0
Peroxisomes 0 0 0.16 0.07 0 0.07 0.1 0.12 0.07 0.07 0.05 0.13 0.08 0.08 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 0 0.22 0.17 0.15 0.12 0.15 0.21 0.08 0.1 0.08 0.12 0.22 0.26 0.28 0.13 0.14 0.13 0 0 0 0.06 0 0
Vac/Vac Membrane 0.29 0.32 0.1 0.09 0.15 0.1 0.08 0.09 0.07 0.09 0.09 0.13 0.16 0.3 0.19 0.41 0.38 0.41 0.05 0.09 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 3 4 2 2 2 3 0 0 1 2 0 1 0 0 1 0 1 2
Bud 0 0 12 6 6 24 35 50 28 44 36 35 2 1 0 2 0 2 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 0 2 2 2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 2 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 23 15 34 33 14 29 18 9 8 16 4 6 6 6 11 7 0 0 0 0 0 1
Endoplasmic Reticulum 0 2 0 0 1 2 2 4 7 7 3 1 0 1 2 1 1 7 0 0 2 0 1 3
Endosome 120 34 524 316 468 300 322 388 192 230 116 414 74 209 207 69 90 78 170 130 624 133 260 305
Golgi 1 2 2 2 3 3 1 6 2 7 0 5 0 2 4 7 8 7 5 2 17 1 3 6
Mitochondria 53 76 164 222 212 453 534 350 502 582 495 558 16 5 7 20 18 10 4 10 6 2 9 9
Nucleus 0 4 0 0 7 2 13 7 2 4 1 4 2 1 1 1 1 1 0 1 0 0 2 1
Nuclear Periphery 9 16 14 11 5 12 24 16 17 15 8 15 7 5 7 9 2 7 3 1 10 0 1 3
Nucleolus 85 44 161 119 130 122 124 142 79 100 74 84 44 65 65 25 30 36 1 1 1 1 1 0
Peroxisomes 2 0 111 38 21 61 97 117 55 65 36 135 10 21 11 1 2 0 4 1 16 2 6 5
SpindlePole 19 4 153 88 117 99 151 210 59 94 56 133 26 69 74 16 21 18 8 4 27 9 9 4
Vac/Vac Membrane 57 49 72 44 112 89 79 90 52 81 62 138 19 81 51 52 57 58 9 16 6 6 12 20
Unique Cell Count 198 154 700 517 756 848 989 1007 755 917 690 1074 118 269 264 126 152 140 212 173 723 161 314 367
Labelled Cell Count 348 237 1236 861 1116 1201 1400 1419 1015 1242 900 1546 204 466 436 211 241 232 212 173 723 161 314 367


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.7 7.1 6.8 6.4 5.3 5.4 6.2 5.8 6.0 5.8 6.0 8.3 8.0 8.1 9.2 8.7 9.1 7.0 6.9 7.4
Std Deviation (1e-4) 0.9 1.2 1.2 1.4 1.5 1.5 1.0 1.4 1.3 1.1 1.1 1.1 9.2 0.9 1.1 1.9 1.7 1.7 0.9 1.1 1.2
Intensity Change (Log2) -0.07 -0.14 -0.42 -0.38 -0.2 -0.28 -0.25 -0.29 -0.24 0.23 0.17 0.19 0.38 0.3 0.36 -0.02 -0.04 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.8 -1.6 1.4 2.2 3.5 2.3 3.4 3.6 2.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 1.2 0.6 -2.6 -0.5 -1.0 -3.3 -2.7 -2.5 0 -0.9 -0.8 0 2.3 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -5.1 -5.3 -15.5 -17.1 -14.8 -18.8 -19.9 -21.7 -15.0 -2.8 0.9 1.1 -4.6 -3.9 -4.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 7.2 2.0 12.0 12.6 5.0 16.5 16.0 18.0 12.0 -2.4 -7.9 -7.5 -1.9 -3.2 -4.3
Nucleus 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.2 -2.2 -0.9 0.6 -0.6 0.3 -0.5 -1.3 -1.0 0 -0.1 0.6 0 0 0
Nucleolus 0 -2.8 -4.4 -5.7 -4.7 -6.4 -6.5 -6.1 -9.1 3.3 0.4 0.5 -0.8 -0.9 0.7
Peroxisomes -4.5 -8.7 -5.4 -3.7 -2.5 -5.1 -5.6 -6.4 -2.0 -2.1 -3.3 -4.9 -4.5 -4.8 -5.1
SpindlePole -2.1 -3.1 -5.4 -3.5 -0.5 -7.6 -6.4 -7.2 -5.3 0 1.3 2.0 -2.3 -2.2 -2.4
Vacuole -1.0 2.6 0.1 -1.6 -0.9 -2.3 -1.0 -0.8 1.6 1.9 7.6 3.7 9.0 8.5 9.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pep3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available