Standard name
Description Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.08 0.06 0.06 0 0.05 0 0 0.05 0 0.1 0.07 0.13 0.05 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.11 0 0.07 0 0.13 0.14 0.09 0.31 0.26 0.37 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.86 0.88 0.89 0.88 0.91 0.89 0.87 0.88 0.8 0.81 0.75 0.86 0.88 0.84 0.8 0.8 0.76 0.82 0.74 0.82 0.75 0.64 0.51 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.11 0 0 0 0.06 0.12 0.1 0.24 0.26 0.23 0.18 0.16 0.13 0.17 0.11 0.07 0.08 0.11 0.05 0.1 0.13 0.19 0.16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.1 0 0 0 0.08 0.13 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 4
Bud 1 0 0 0 0 0 2 2 4 3 4 4 0 0 0 0 0 0 1 4 3 0 4 6
Bud Neck 0 0 0 0 1 0 1 2 0 0 2 6 0 0 0 0 0 0 1 0 2 0 0 3
Bud Site 1 0 0 1 1 9 8 7 8 13 14 9 1 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 8 5 13 14 13 19 16 20 9 8 11 5 19 14 25 8 10 4 3 0 0 0 2 5
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 5 2 3 0 0 0 1 1 2
Endosome 3 4 2 0 0 0 0 1 0 0 0 0 0 0 3 3 5 0 3 4 2 1 3 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 1 2 1 1 2 2
Mitochondria 24 25 2 12 2 43 54 39 71 80 87 42 3 1 2 7 1 3 1 2 1 0 3 3
Nucleus 186 205 202 157 200 300 328 366 183 247 177 265 166 169 159 136 118 119 174 197 176 42 92 91
Nuclear Periphery 2 1 0 1 0 4 0 1 2 2 4 2 2 1 1 1 0 1 2 0 0 0 1 1
Nucleolus 36 26 2 7 6 21 44 43 56 80 55 56 30 27 34 19 11 11 26 12 24 9 34 29
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
SpindlePole 0 1 3 2 3 5 5 12 1 1 1 3 1 1 1 0 2 4 8 2 9 1 3 5
Vac/Vac Membrane 9 11 8 3 2 2 2 6 0 0 2 2 0 2 2 12 13 14 10 9 9 5 24 20
Unique Cell Count 217 232 227 178 220 337 377 414 230 304 237 308 189 201 198 169 156 145 236 241 235 67 180 190
Labelled Cell Count 271 279 234 197 228 403 460 499 334 434 357 394 222 217 227 193 165 160 236 241 235 67 180 190


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.9 6.0 5.6 6.3 5.3 5.1 5.6 4.8 4.7 4.4 4.9 6.4 6.7 6.5 9.1 9.5 8.9 5.8 5.9 7.4
Std Deviation (1e-4) 1.1 1.1 1.0 1.1 1.3 1.7 1.1 1.4 1.2 1.2 1.2 0.9 2.0 1.8 1.6 2.1 2.4 1.9 0.9 1.6 3.9
Intensity Change (Log2) -0.09 0.08 -0.18 -0.22 -0.1 -0.31 -0.35 -0.43 -0.28 0.1 0.16 0.12 0.6 0.67 0.57 -0.04 -0.03 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.1 0 -0.8 -0.5 -0.9 -1.8 -0.5 -2.6 1.6 0.5 2.5 -0.4 0.3 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.2 0 5.1 5.5 4.2 0 0 0 5.3 0 0 0 0 0 0
Nucleus -0.2 0.7 0 -0.7 -0.2 -2.8 -2.4 -4.0 -1.0 -0.4 -1.5 -2.5 -2.4 -3.5 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.2 4.8 4.5 0 0 0 6.4 5.7 5.2 6.0 4.6 3.3 3.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1.3 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.6 2.0 2.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swi6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available