Standard name
Human Ortholog
Description Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.08 0.06 0.06 0 0.05 0 0 0.05 0 0.1 0.07 0.13 0.05 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.11 0 0.07 0 0.13 0.14 0.09 0.31 0.26 0.37 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.86 0.88 0.89 0.88 0.91 0.89 0.87 0.88 0.8 0.81 0.75 0.86 0.88 0.84 0.8 0.8 0.76 0.82 0.74 0.82 0.75 0.64 0.51 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.11 0 0 0 0.06 0.12 0.1 0.24 0.26 0.23 0.18 0.16 0.13 0.17 0.11 0.07 0.08 0.11 0.05 0.1 0.13 0.19 0.16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.1 0 0 0 0.08 0.13 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 4
Bud 1 0 0 0 0 0 2 2 4 3 4 4 0 0 0 0 0 0 1 4 3 0 4 6
Bud Neck 0 0 0 0 1 0 1 2 0 0 2 6 0 0 0 0 0 0 1 0 2 0 0 3
Bud Site 1 0 0 1 1 9 8 7 8 13 14 9 1 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 8 5 13 14 13 19 16 20 9 8 11 5 19 14 25 8 10 4 3 0 0 0 2 5
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 5 2 3 0 0 0 1 1 2
Endosome 3 4 2 0 0 0 0 1 0 0 0 0 0 0 3 3 5 0 3 4 2 1 3 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 1 2 1 1 2 2
Mitochondria 24 25 2 12 2 43 54 39 71 80 87 42 3 1 2 7 1 3 1 2 1 0 3 3
Nucleus 186 205 202 157 200 300 328 366 183 247 177 265 166 169 159 136 118 119 174 197 176 42 92 91
Nuclear Periphery 2 1 0 1 0 4 0 1 2 2 4 2 2 1 1 1 0 1 2 0 0 0 1 1
Nucleolus 36 26 2 7 6 21 44 43 56 80 55 56 30 27 34 19 11 11 26 12 24 9 34 29
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
SpindlePole 0 1 3 2 3 5 5 12 1 1 1 3 1 1 1 0 2 4 8 2 9 1 3 5
Vac/Vac Membrane 9 11 8 3 2 2 2 6 0 0 2 2 0 2 2 12 13 14 10 9 9 5 24 20
Unique Cell Count 217 232 227 178 220 337 377 414 230 304 237 308 189 201 198 169 156 145 236 241 235 67 180 190
Labelled Cell Count 271 279 234 197 228 403 460 499 334 434 357 394 222 217 227 193 165 160 236 241 235 67 180 190


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.9 6.0 5.6 6.3 5.3 5.1 5.6 4.8 4.7 4.4 4.9 6.4 6.7 6.5 9.1 9.5 8.9 5.8 5.9 7.4
Std Deviation (1e-4) 1.1 1.1 1.0 1.1 1.3 1.7 1.1 1.4 1.2 1.2 1.2 0.9 2.0 1.8 1.6 2.1 2.4 1.9 0.9 1.6 3.9
Intensity Change (Log2) -0.09 0.08 -0.18 -0.22 -0.1 -0.31 -0.35 -0.43 -0.28 0.1 0.16 0.12 0.6 0.67 0.57 -0.04 -0.03 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.1 0 -0.8 -0.5 -0.9 -1.8 -0.5 -2.6 1.6 0.5 2.5 -0.4 0.3 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.2 0 5.1 5.5 4.2 0 0 0 5.3 0 0 0 0 0 0
Nucleus -0.2 0.7 0 -0.7 -0.2 -2.8 -2.4 -4.0 -1.0 -0.4 -1.5 -2.5 -2.4 -3.5 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.2 4.8 4.5 0 0 0 6.4 5.7 5.2 6.0 4.6 3.3 3.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1.3 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.6 2.0 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4284 4.3153 4.2054 3.529 4.338 3.911 2.6457 2.6457 2.4154 2.0137 1.7227 2.0909 2.3882 2.3443 2.0974 2.4727 3.6322 1.7878
Actin 0.0831 0.0001 0.002 0.0079 0.0017 0.0077 0.0216 0.0001 0.0034 0.0044 0.0001 0.0023 0.0286 0 0.0125 0.0065 0.0243 0
Bud 0.0012 0.0003 0.0002 0.0031 0.0001 0.0001 0.0003 0 0.0007 0.0022 0.0001 0.0002 0.0009 0 0.0007 0.0002 0.0037 0
Bud Neck 0.0077 0.0003 0.0013 0.0027 0.0018 0.0019 0.0008 0.0001 0.0004 0.0009 0.001 0.0011 0.0015 0.0001 0.0004 0.0009 0.0016 0.0012
Bud Periphery 0.0023 0.0008 0.0003 0.0058 0.0001 0.0005 0.0005 0.0001 0.0007 0.0054 0.0001 0.0006 0.0018 0 0.0021 0.0003 0.0037 0.0001
Bud Site 0.0174 0.0062 0.0013 0.0384 0.0003 0.0004 0.0011 0.0001 0.005 0.0015 0.0005 0.0002 0.0038 0.0001 0.0005 0.0007 0.004 0.0001
Cell Periphery 0.0006 0.001 0.0002 0.0016 0 0.0001 0.0002 0 0.0001 0.0003 0 0.0001 0.0006 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.0038 0.0002 0.001 0.0024 0.0057 0.0001 0.0038 0.0001 0.0035 0.0023 0.0016 0.0006 0.0013 0.0001 0.0001 0.0004 0.0052 0.0009
Cytoplasmic Foci 0.0227 0 0.0027 0.0101 0.0007 0.0004 0.0226 0 0.0115 0.0006 0.033 0.0008 0.0234 0 0.0002 0.0094 0.0159 0.0003
Eisosomes 0.0006 0 0.0002 0.0003 0 0.0001 0.0002 0 0 0.0001 0 0 0.0002 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0047 0.0001 0.0005 0.0006 0.0032 0.0008 0.0047 0 0.0009 0.0017 0.0006 0.0015 0.0027 0 0.0003 0.0001 0.0018 0
Endosome 0.0346 0.0001 0.002 0.0066 0.0122 0.0015 0.0584 0 0.0081 0.0059 0.0322 0.0041 0.0227 0 0.0012 0.0058 0.0265 0.0001
Golgi 0.0169 0 0.001 0.0052 0.0005 0.0008 0.0152 0 0.0038 0.0012 0.0034 0.0011 0.0112 0 0.0008 0.0109 0.0107 0
Lipid Particles 0.0571 0.0001 0.0009 0.0188 0.0008 0.0029 0.0286 0 0.006 0.0007 0.0144 0.0012 0.0305 0 0.0006 0.05 0.0022 0
Mitochondria 0.0125 0.0006 0.0035 0.0043 0.0005 0.0029 0.025 0.0018 0.0027 0.0141 0.0004 0.0066 0.0125 0.0002 0.0026 0.0036 0.0194 0.0001
None 0.006 0.0001 0.0096 0.0013 0.0021 0.0004 0.0033 0 0.0006 0.0009 0.0001 0.0002 0.0012 0 0.0001 0.0002 0.0025 0.0002
Nuclear Periphery 0.0143 0.0079 0.0021 0.003 0.05 0.0014 0.0192 0.0076 0.009 0.0257 0.0034 0.0025 0.0038 0.0075 0.0013 0.0029 0.0039 0.0007
Nucleolus 0.0246 0.0299 0.0288 0.009 0.073 0.1279 0.0366 0.0348 0.0329 0.039 0.1071 0.1031 0.0258 0.0296 0.0195 0.0348 0.0314 0.0837
Nucleus 0.6257 0.948 0.9324 0.8627 0.7702 0.8459 0.736 0.9538 0.9013 0.8839 0.7922 0.8686 0.772 0.9619 0.9529 0.8452 0.7142 0.8986
Peroxisomes 0.0372 0 0.0053 0.0067 0.0001 0.0013 0.0062 0 0.0042 0.0007 0.0006 0.0011 0.0459 0 0.0004 0.0232 0.0075 0
Punctate Nuclear 0.0139 0.0019 0.0039 0.0063 0.0749 0.0016 0.0059 0.001 0.0034 0.003 0.0076 0.0029 0.004 0.0004 0.0029 0.0038 0.1194 0.0138
Vacuole 0.0104 0.0015 0.0007 0.0028 0.0011 0.0007 0.0065 0.0001 0.001 0.0026 0.0013 0.0006 0.0041 0 0.0004 0.0006 0.0011 0.0001
Vacuole Periphery 0.0029 0.001 0.0002 0.0004 0.0008 0.0003 0.0035 0.0002 0.0007 0.0027 0.0002 0.0006 0.0013 0.0001 0.0003 0.0002 0.0008 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5538 15.9489 13.0486 15.8848 18.0902 15.5452 18.7102 18.5584 17.2624 22.5457
Translational Efficiency 1.8648 1.4965 1.0946 0.9255 1.1453 1.4961 1.0891 0.7697 0.9273 0.962

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Swi6

Swi6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swi6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available