Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.42 0.47 0.3 0.17 0.21 0.19 0.12 0.1 0.11 – 0.17 0.14 0.25 0.2 0.15 0.41 0.3 0.2 0.19 0.17 0.15 0.09 0.1 0.07
Bud 0.15 0.25 0.2 0.11 0.2 0.16 0.16 0.12 0.13 – 0.15 0.1 0.14 0.07 0.08 0.19 0.15 0.08 0.32 0.32 0.23 0.08 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.14 0.22 0.23 0.16 0.05 0
Bud Site 0.09 0.08 0.12 0 0 0.05 0 0 0.05 – 0 0.05 0 0 0 0.07 0 0 – – – – – –
Cell Periphery 0.19 0.18 0.14 0.21 0.09 0.17 0.12 0.09 0.22 – 0.23 0.21 0.4 0.5 0.5 0.19 0.28 0.36 0.16 0.11 0.11 0 0 0
Cytoplasm 0.35 0.36 0.46 0.71 0.59 0.51 0.51 0.5 0.46 – 0.4 0.4 0.57 0.63 0.69 0.33 0.38 0.5 0.09 0.1 0.15 0.59 0.68 0.72
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.05 0.21 0.25 0.35 0.4 – 0.44 0.47 0 0 0 0.08 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.12 0 0.12 0 0.05 0.05 0 0 0 – 0 0 0 0 0 0.11 0.05 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 118 149 156 60 81 103 77 75 75 – 79 57 116 145 114 31 39 26 63 63 89 42 52 47
Bud 42 79 102 40 78 84 100 90 87 – 70 41 66 53 58 14 20 10 105 116 137 39 30 22
Bud Neck 1 3 13 1 5 3 9 10 5 – 2 1 0 1 5 1 6 0 48 79 135 76 28 27
Bud Site 25 26 59 10 17 28 21 24 34 – 6 18 6 3 1 5 3 0 – – – – – –
Cell Periphery 54 55 70 74 36 91 78 66 145 – 104 84 186 364 384 14 36 47 54 41 66 5 10 26
Cytoplasm 99 114 236 254 231 273 325 364 307 – 185 160 270 463 534 25 49 65 28 35 89 288 362 460
Endoplasmic Reticulum 5 17 8 4 15 16 18 15 14 – 11 18 5 10 9 2 6 7 7 2 7 9 15 14
Endosome 1 1 4 2 8 6 1 8 2 – 0 1 0 0 0 0 0 0 3 3 4 6 4 7
Golgi 2 0 0 2 0 0 0 0 0 – 0 0 0 1 0 1 1 0 5 0 0 1 1 1
Mitochondria 3 9 20 23 21 110 156 255 263 – 203 186 2 5 5 6 4 5 5 9 25 6 9 10
Nucleus 12 3 14 2 4 6 12 16 4 – 3 5 3 3 2 2 5 4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 2 3 4 – 1 4 1 1 5 1 0 0 1 0 2 0 0 0
Nucleolus 0 0 0 1 3 2 3 8 4 – 2 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 7 5 11 14 9 15 17 9 20 – 2 4 4 2 2 1 3 4 0 0 0 2 0 1
SpindlePole 34 13 60 16 19 26 25 27 18 – 1 3 13 9 8 8 7 6 1 0 7 0 1 2
Vac/Vac Membrane 3 5 7 2 2 4 8 17 9 – 3 4 2 1 0 4 10 3 2 12 15 7 3 8
Unique Cell Count 280 314 512 357 391 531 634 723 664 458 398 470 733 770 75 130 130 333 371 585 493 533 641
Labelled Cell Count 406 479 760 505 529 768 852 987 991 672 586 674 1061 1127 115 190 177 333 371 585 493 533 641


Ambiguous, Bud, Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 6.3 5.0 5.1 4.9 4.7 4.4 4.4 4.1 – 4.1 4.1 6.4 6.7 5.9 9.1 9.4 9.5 6.8 6.9 7.2
Std Deviation (1e-4) 1.5 1.1 0.8 1.3 1.1 1.0 1.0 1.0 1.0 – 0.8 0.9 1.1 1.1 1.0 1.4 1.9 1.9 1.3 1.2 1.1
Intensity Change (Log2) – – – 0.03 -0.02 -0.1 -0.18 -0.18 -0.29 – -0.28 -0.28 0.36 0.43 0.24 0.87 0.91 0.93 0.44 0.46 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.6 -3.3 0 0 0 0 – 0 0 -2.0 -4.3 -6.7 1.9 -0.1 -2.4
Bud -3.4 0 0 0 0 0 – 0 0 -2.4 -6.7 -6.6 -0.3 -1.2 -3.3
Bud Neck -2.6 -1.3 0 0 0 0 – 0 0 -3.5 -4.0 -2.8 0 0 0
Bud Site -4.7 -3.8 0 0 0 0 – 0 0 -6.5 -8.9 -9.5 -1.3 -3.2 -4.1
Cell Periphery 2.8 -2.1 0 0 0 0 – 0 0 9.2 13.1 13.3 1.2 3.8 5.9
Cytoplasm 7.3 3.9 1.7 1.7 1.5 0 – -1.8 -1.8 3.6 6.0 8.3 -2.1 -1.7 0.8
Endoplasmic Reticulum 0 2.1 0 0 0 0 – 0 0 -0.7 -0.3 -0.6 0 0 0
Endosome 0 1.6 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1.7 1.0 0 0 0 0 – 0 0 -3.7 -4.0 -4.1 0 0 0
Nucleus -2.3 -1.8 -1.9 -1.0 -0.6 -3.0 – -2.5 -1.5 -2.5 -3.5 -3.9 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 1.5 0.2 0 0 0 0 – 0 0 -1.7 -3.2 -3.3 0 0 0
SpindlePole -3.7 -3.6 0 0 0 0 – 0 0 -5.3 -8.0 -8.4 -0.3 -2.1 -2.4
Vacuole 0 0 -1.0 -0.2 1.2 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4502 3.2194 3.2936 3.3701 2.9847 3.9385 0.8795 0.9591 1.1711 1.1737 0.9931 1.4997 6.2734 6.3689 6.7607 7.3269 6.5929 6.8005
Actin 0.0757 0.0171 0.0335 0.1152 0.0347 0.0277 0.0264 0.018 0.0078 0.0134 0.04 0.0173 0.019 0.0114 0.0131 0.0099 0.0337 0.0363
Bud 0.0484 0.0537 0.3106 0.0846 0.066 0.0194 0.0704 0.0808 0.3416 0.0887 0.0855 0.0267 0.0467 0.0889 0.2727 0.1052 0.1165 0.0267
Bud Neck 0.0467 0.0124 0.0264 0.0191 0.1023 0.5705 0.0289 0.0051 0.001 0.0041 0.0037 0.4892 0.0476 0.0074 0.0062 0.0106 0.04 0.4441
Bud Periphery 0.1656 0.0737 0.4882 0.5485 0.5585 0.0817 0.2116 0.1389 0.5071 0.6145 0.7243 0.1587 0.1878 0.147 0.519 0.73 0.6598 0.122
Bud Site 0.2333 0.3593 0.0618 0.0413 0.01 0.0145 0.1846 0.3733 0.073 0.0216 0.0047 0.0287 0.2737 0.3674 0.0899 0.0153 0.0299 0.0266
Cell Periphery 0.124 0.0746 0.0155 0.0327 0.0421 0.0544 0.13 0.0324 0.0106 0.038 0.0505 0.0287 0.2046 0.05 0.0287 0.0298 0.0433 0.0663
Cytoplasm 0.1416 0.2688 0.0262 0.03 0.0213 0.1776 0.2727 0.2464 0.0307 0.1247 0.0591 0.1896 0.1706 0.2464 0.046 0.0403 0.0694 0.2261
Cytoplasmic Foci 0.0103 0.0025 0.0026 0.0095 0.0472 0.0058 0.0084 0.0077 0.001 0.0028 0.0011 0.0111 0.0079 0.0037 0.001 0.0045 0.0006 0.0086
Eisosomes 0.0033 0.0011 0.0003 0.0019 0.0011 0.0002 0.0026 0.0021 0.0001 0.0005 0.0034 0.0007 0.0015 0.0007 0.0003 0.0002 0.0002 0.0005
Endoplasmic Reticulum 0.004 0.0029 0.0011 0.0029 0.0033 0.009 0.0064 0.0044 0.001 0.0131 0.0055 0.0034 0.0038 0.0036 0.0009 0.0009 0.0009 0.002
Endosome 0.0151 0.0018 0.0033 0.0061 0.0062 0.0099 0.0027 0.0044 0.0006 0.0026 0.0007 0.0041 0.0031 0.0014 0.0004 0.0022 0.0003 0.0012
Golgi 0.0081 0.0006 0.0017 0.0114 0.0093 0.0056 0.0015 0.0013 0.0002 0.0007 0.0006 0.0022 0.0013 0.0007 0.0003 0.0005 0.0002 0.0011
Lipid Particles 0.0118 0.0008 0.0014 0.018 0.0098 0.004 0.0017 0.0008 0.0001 0.0005 0.0002 0.0012 0.0015 0.0004 0.0001 0.0002 0 0.0006
Mitochondria 0.0097 0.0004 0.0019 0.0124 0.0072 0.0019 0.0013 0.002 0.001 0.0023 0.0018 0.0038 0.0012 0.0009 0.0025 0.0018 0.001 0.0008
None 0.0533 0.114 0.012 0.0083 0.0671 0.0018 0.0333 0.0669 0.0136 0.0434 0.0107 0.0193 0.0138 0.0605 0.0144 0.0355 0.0014 0.0257
Nuclear Periphery 0.0045 0.0004 0.0025 0.005 0.0024 0.0074 0.0008 0.0009 0.0005 0.0026 0.0017 0.0006 0.0012 0.0008 0.0002 0.0006 0.0002 0.0003
Nucleolus 0.0016 0.0006 0.0006 0.0007 0.0005 0.0004 0.0008 0.0011 0.0005 0.0015 0.0002 0.0016 0.0008 0.0007 0.0005 0.0008 0.0002 0.0008
Nucleus 0.0144 0.0016 0.0014 0.0009 0.0012 0.0013 0.0018 0.0041 0.0016 0.006 0.0019 0.0024 0.002 0.0041 0.0009 0.0021 0.0007 0.0014
Peroxisomes 0.0094 0.0002 0.0012 0.0482 0.0023 0.0012 0.0006 0.0008 0.0002 0.0004 0.0002 0.0011 0.001 0.0002 0.0002 0.0004 0.0002 0.0017
Punctate Nuclear 0.0039 0.0004 0.0025 0.0013 0.0021 0.0014 0.0005 0.0012 0.0002 0.0009 0.0003 0.001 0.0015 0.0005 0.0001 0.0018 0.0001 0.0014
Vacuole 0.0139 0.0126 0.0049 0.0013 0.0043 0.0034 0.0125 0.0071 0.0067 0.0161 0.0036 0.008 0.009 0.003 0.0024 0.0067 0.0012 0.0054
Vacuole Periphery 0.0013 0.0004 0.0003 0.0008 0.0009 0.0007 0.0006 0.0004 0.0009 0.0016 0.0005 0.0007 0.0004 0.0002 0.0002 0.0006 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.2647 19.2089 12.1157 14.9131 13.4657 11.0959 20.3932 20.646 15.909 18.4689
Translational Efficiency 1.1128 1.117 1.3601 0.8415 0.9256 1.7836 0.9999 0.8187 1.0138 0.9127

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
Localization
Cell Percentages bud neck (17%), cytoplasm (15%), mixed (34%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (7.4e-11)
bud neck - S/G2 (9e-07)
bud neck - MA (7.4e-05)
Subcompartmental Group N/A

Skg3

Skg3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Skg3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available