Standard name
Human Ortholog
Description Mitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.05
Bud 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.53 0.37 0.31 0.34 0.24 0.14 0.14 0.1 – 0.07 0 0 0.77 0.82 0.82 0.38 0.41 0.2 0.23 0.17 0.1 0.15 0.17 0.21
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0.05 0 0.05 0 0.1 0 0 0 – 0 0 0 0.05 0 0.07 0.19 0.19 0.4 0.05 0.07 0.11 0.08 0.07 0.07
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
Mitochondria 0.37 0.62 0.59 0.62 0.58 0.87 0.81 0.86 – 0.93 0.94 0.93 0.06 0.05 0 0.14 0.12 0.05 0.52 0.54 0.59 0.51 0.47 0.42
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0.08 0.2 0 0 0.06 0.05 0.05 0
SpindlePole 0 0 0.07 0 0.07 0 0.08 0.07 – 0.05 0 0.07 0 0 0 0.06 0.06 0.15 0 0 0 0 0.06 0.06
Vac/Vac Membrane 0.06 0 0 0 0.1 0.07 0 0 – 0 0 0 0.05 0 0.07 0.24 0.29 0.25 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 9 0 1 0 0 0 0 – 0 0 0 2 7 2 0 0 1 6 10 11 18 28 31
Bud 2 14 7 10 3 8 22 28 – 31 13 28 1 1 1 0 0 0 2 6 2 7 17 16
Bud Neck 1 1 2 0 1 0 0 2 – 1 0 1 3 4 1 0 0 0 1 9 4 8 9 21
Bud Site 1 2 1 0 1 1 13 12 – 22 8 14 0 0 0 1 0 0 – – – – – –
Cell Periphery 2 3 3 0 1 2 5 2 – 2 5 8 3 0 2 2 1 1 0 2 0 3 5 6
Cytoplasm 94 140 101 68 82 57 87 76 – 47 16 26 179 191 210 41 49 4 53 73 38 76 103 136
Endoplasmic Reticulum 1 23 2 1 1 3 1 1 – 2 1 0 2 5 5 3 6 0 3 2 2 5 4 5
Endosome 9 0 16 8 35 11 11 13 – 0 1 2 12 6 18 21 23 8 11 30 39 38 40 44
Golgi 0 0 1 0 1 0 0 0 – 0 0 0 2 2 1 1 0 2 3 5 7 6 7 6
Mitochondria 65 237 193 125 193 365 510 676 – 649 471 642 15 12 1 15 14 1 121 232 219 253 287 274
Nucleus 0 1 0 0 3 4 11 19 – 5 6 11 1 1 2 5 7 0 0 0 0 1 2 0
Nuclear Periphery 1 1 0 0 5 5 16 17 – 21 18 27 0 0 0 0 0 0 1 0 1 2 3 4
Nucleolus 0 1 0 1 3 3 17 32 – 33 9 19 0 0 0 0 0 0 0 1 0 1 3 3
Peroxisomes 5 0 26 9 20 6 15 28 – 6 2 12 5 0 4 4 10 4 5 5 21 22 31 25
SpindlePole 5 6 24 8 22 8 53 58 – 34 17 46 6 2 2 7 7 3 8 15 12 21 38 40
Vac/Vac Membrane 10 14 13 3 35 30 28 17 – 20 12 23 12 8 17 26 35 5 4 18 3 13 17 22
Unique Cell Count 177 382 326 200 335 420 633 789 699 499 689 231 234 256 109 120 20 233 429 372 493 617 654
Labelled Cell Count 196 452 389 234 406 503 789 981 873 579 859 243 239 266 126 152 29 233 429 372 493 617 654


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.8 3.9 3.9 4.1 3.6 3.5 3.8 – 3.4 3.7 3.8 5.2 5.3 5.5 5.9 6.8 8.2 5.4 5.6 5.6
Std Deviation (1e-4) 1.0 0.6 1.1 1.3 1.5 1.2 1.2 1.2 – 1.0 1.1 1.1 1.1 1.0 1.1 1.6 1.9 1.4 1.4 1.7 1.6
Intensity Change (Log2) – – – 0.01 0.08 -0.11 -0.15 -0.02 – -0.18 -0.06 -0.03 0.44 0.46 0.52 0.62 0.82 1.09 0.48 0.54 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 6.3 7.6 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0086 0.0132 0.0386 0.0057 0.0068 0.0346 0.0577 0.0062 0.0277 0.0743 0.0222 0.0202 – – – – – –
Bud 0.001 0.0031 0.0045 0.002 0.0031 0.0041 0.0014 0.0023 0.002 0.0048 0.0004 0.0015 – – – – – –
Bud Neck 0.002 0.002 0.0009 0.0033 0.0012 0.0063 0.0043 0.0016 0.0033 0.0006 0.0003 0.0264 – – – – – –
Bud Periphery 0.0017 0.0046 0.0197 0.0059 0.009 0.011 0.003 0.004 0.0059 0.0085 0.0004 0.0036 – – – – – –
Bud Site 0.0141 0.0493 0.013 0.0088 0.0215 0.0033 0.0219 0.0301 0.0131 0.0047 0.0006 0.0023 – – – – – –
Cell Periphery 0.0009 0.0013 0.001 0.0044 0.0006 0.0011 0.0039 0.0022 0.0028 0.0004 0.0003 0.0013 – – – – – –
Cytoplasm 0.0344 0.0592 0.0577 0.0427 0.0383 0.0539 0.0669 0.1376 0.0546 0.0419 0.026 0.0887 – – – – – –
Cytoplasmic Foci 0.1062 0.0814 0.0633 0.0901 0.0942 0.1038 0.175 0.1178 0.0948 0.0627 0.0779 0.1008 – – – – – –
Eisosomes 0.0008 0.0009 0.0007 0.0013 0.0004 0.0008 0.0014 0.0007 0.0015 0.0002 0.0009 0.0006 – – – – – –
Endoplasmic Reticulum 0.0055 0.0056 0.0064 0.0038 0.0024 0.0054 0.004 0.0058 0.003 0.0036 0.0005 0.0052 – – – – – –
Endosome 0.1419 0.1101 0.1298 0.0907 0.1461 0.1003 0.1016 0.0802 0.1013 0.1101 0.0691 0.0676 – – – – – –
Golgi 0.039 0.0624 0.0563 0.0364 0.0666 0.0624 0.0347 0.0315 0.0541 0.0246 0.0409 0.0529 – – – – – –
Lipid Particles 0.091 0.0211 0.0221 0.0412 0.0266 0.0507 0.0459 0.0302 0.025 0.0506 0.0313 0.0131 – – – – – –
Mitochondria 0.2339 0.2938 0.3727 0.4286 0.3662 0.2937 0.1076 0.1508 0.2611 0.27 0.1594 0.2641 – – – – – –
None 0.2685 0.2301 0.155 0.1418 0.1331 0.1925 0.2608 0.2701 0.2756 0.2327 0.5313 0.2882 – – – – – –
Nuclear Periphery 0.0038 0.0016 0.0026 0.0017 0.0007 0.0023 0.0025 0.001 0.0009 0.0015 0.0001 0.0009 – – – – – –
Nucleolus 0.0009 0.0013 0.0005 0.009 0.0003 0.0019 0.004 0.0013 0.0014 0.0004 0.0001 0.0008 – – – – – –
Nucleus 0.0011 0.0017 0.0012 0.0046 0.0006 0.0036 0.0027 0.0021 0.0008 0.0007 0.0002 0.001 – – – – – –
Peroxisomes 0.0268 0.0394 0.0255 0.0594 0.0659 0.0511 0.0791 0.0896 0.0588 0.0854 0.035 0.052 – – – – – –
Punctate Nuclear 0.0043 0.0023 0.0014 0.0013 0.001 0.005 0.0113 0.0012 0.0009 0.0007 0.0004 0.0012 – – – – – –
Vacuole 0.0084 0.0117 0.0156 0.0137 0.0096 0.0086 0.0079 0.0266 0.0088 0.0129 0.0017 0.0053 – – – – – –
Vacuole Periphery 0.0052 0.0041 0.0112 0.0034 0.0056 0.0039 0.0024 0.0071 0.0027 0.0087 0.0009 0.0024 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.5769 33.5406 22.7853 27.299 33.9154 25.3516 28.7781 27.5634 31.1316 40.1197
Translational Efficiency 0.4514 0.3731 0.4667 0.4251 0.422 0.658 0.3603 0.3515 0.3363 0.3498

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
434 1157 175 568 1364 2161 3157 1309 1798 3318 3332 1877

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 834.87 671.73 930.63 855.98 706.55 722.56 789.60 810.65 737.52 704.84 797.01 824.37
Standard Deviation 134.14 97.60 101.36 109.01 81.50 90.23 101.84 107.68 111.34 95.97 106.57 110.07
Intensity Change Log 2 — -0.313670 0.156656 0.036026 — 0.032326 0.160330 0.198288 — -0.144729 0.158341 0.112674

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007279 0.001176 0.007057 0.025962 0.001859 0.001077 0.002749 0.008304 0.003168 0.001111 0.002975 0.013648
Bud Neck 0.073324 0.058507 0.010940 0.022569 0.027770 0.048105 0.089506 0.023764 0.038766 0.051732 0.085379 0.023402
Bud Site 0.113053* 0.039944 0.025803 0.107922* 0.017992 0.030881 0.081051 0.053749 0.040937 0.034041 0.078150 0.070142
Cell Periphery 0.003983 0.000221 0.000271 0.000541 0.000640 0.000226 0.000248 0.000239 0.001447 0.000225 0.000249 0.000330
Cytoplasm 0.151185* 0.201295* 0.149735* 0.266760* 0.077626 0.140989* 0.212252* 0.309860* 0.095381 0.162018* 0.208968* 0.296818*
Cytoplasmic Foci 0.158381 0.224688* 0.111986 0.109226 0.180773* 0.236541* 0.054412 0.113340 0.175368 0.232408* 0.057436 0.112095
Eisosomes 0.000507 0.000150 0.000157 0.000239 0.000477 0.000197 0.000090 0.000151 0.000484 0.000181 0.000093 0.000177
Endoplasmic Reticulum 0.002952 0.000942 0.009614 0.005756 0.001159 0.000537 0.002089 0.002232 0.001592 0.000679 0.002484 0.003299
Endosome 0.092578 0.071769 0.041744 0.021655 0.060070 0.073349 0.036748 0.036985 0.067917 0.072798 0.037011 0.032346
Golgi 0.045605 0.035630 0.017516 0.014247 0.095005 0.057936 0.013562 0.014067 0.083081 0.050158 0.013770 0.014121
Lipid Particles 0.007417 0.011946 0.014667 0.005559 0.019258 0.009736 0.002658 0.003916 0.016400 0.010506 0.003289 0.004413
Mitochondria 0.065174 0.117308* 0.007860 0.023111 0.391171* 0.214154* 0.138601* 0.025940 0.312482* 0.180383* 0.131734* 0.025084
Mitotic Spindle 0.023575 0.023384 0.056712 0.034434 0.007215 0.018563 0.088469 0.033019 0.011164 0.020245 0.086801 0.033447
None 0.000516 0.003469 0.001639 0.002444 0.000706 0.001266 0.001455 0.001871 0.000660 0.002035 0.001465 0.002044
Nuclear Periphery 0.000539 0.000764 0.002608 0.000760 0.000221 0.000538 0.001331 0.001388 0.000298 0.000617 0.001398 0.001198
Nuclear Periphery Foci 0.000636 0.000532 0.007422 0.002076 0.000224 0.000262 0.000308 0.000903 0.000323 0.000356 0.000682 0.001258
Nucleolus 0.000803 0.004530 0.003327 0.000410 0.001146 0.001541 0.002094 0.001367 0.001063 0.002583 0.002159 0.001077
Nucleus 0.050624 0.066163 0.378734* 0.113692 0.017512 0.032978 0.099098 0.159941* 0.025505 0.044550 0.113785 0.145946*
Peroxisomes 0.043491 0.054595 0.048995 0.082334 0.064190 0.060799 0.044319 0.061611 0.059194 0.058635 0.044565 0.067882
Vacuole 0.147819 0.073258 0.099707 0.158568 0.025549 0.062897 0.122949 0.143780 0.055062 0.066510 0.121728 0.148255
Vacuole Periphery 0.010559 0.009728 0.003507 0.001736 0.009437 0.007427 0.006011 0.003571 0.009708 0.008229 0.005880 0.003016

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.21 0.55 -2.80 -5.28 -3.11 2.56 -1.77 -3.53 -4.30 -2.98 4.87 0.86 -4.76 -6.76 -5.27
Bud Neck 1.44 7.01 6.50 9.97 -1.52 -6.29 -16.64 2.63 10.20 21.48* -4.09 -12.29 6.38 14.06 22.52
Bud Site 7.28 8.30 3.34 -4.85 -6.27 -5.43 -18.42 -6.40 -2.86 7.75 2.07 -8.30 -2.57 -5.13 5.18
Cell Periphery 4.23 4.15 4.03 -3.74 -2.43 4.31 4.34 4.59 1.00 0.98 5.40 5.38 5.24 -1.66 -1.43
Cytoplasm -3.99 0.51 -7.03 -4.14 -6.42 -10.28 -22.64 -25.41 -17.87 -8.92 -11.82 -19.84 -25.48 -16.75 -9.23
Cytoplasmic Foci -6.51 4.52 7.35 18.29 0.88 -7.98 22.89 12.60 23.02 -10.52 -9.97 24.61 14.81 29.09 -10.68
Eisosomes 3.77 3.55 3.22 -3.17 -1.58 3.80 5.32 4.57 4.90 -7.24 4.98 6.50 5.36 2.51 -9.52
Endoplasmic Reticulum 3.88 -6.32 -4.03 -8.12 3.66 3.51 -4.36 -6.36 -12.18 -1.79 4.94 -4.40 -7.43 -13.10 -3.50
Endosome 2.35 5.86 10.72 15.01 3.71 -3.44 8.50 9.18 13.79 2.27 -1.58 11.02 13.82 18.92 4.63
Golgi 1.53 4.44 6.60 9.17 1.03 7.70 19.46 19.58 17.89 1.69 8.44 19.94 20.49 19.91 2.40
Lipid Particles -2.90 -2.83 1.71 4.69 3.83 5.39 10.08 9.52 8.26 -2.96 4.11 10.08 9.49 9.26 -1.74
Mitochondria -4.94 6.48 5.63 14.17 -1.73 15.73* 26.21 37.87* 30.09* 23.01* 14.20 21.89 35.00* 32.60 23.91*
Mitotic Spindle -0.05 -1.92 -0.18 -0.17 1.84 -5.84 -22.30* -6.04 -2.14 13.40 -4.79 -20.74 -5.24 -1.81 14.41
None -5.38 -3.83 -3.72 1.88 -1.30 -2.56 -5.77 -4.22 -1.66 -1.15 -5.78 -6.81 -5.58 0.51 -1.96
Nuclear Periphery -1.09 -6.71 -6.13 -3.86 4.26 -1.28 -11.72 -10.46 -4.02 0.66 -1.83 -11.93 -12.40 -5.04 1.57
Nuclear Periphery Foci 0.84 -3.62 -8.27 -8.48 2.70 -0.38 -2.64 -7.90 -6.04 -7.02 -0.41 -3.55 -11.58 -9.91 -4.80
Nucleolus -6.27 -2.60 2.50 7.17 3.09 -0.79 -3.04 -0.26 0.81 4.22 -4.10 -4.06 0.27 5.65 6.06
Nucleus -1.45 -12.89 -8.44 -8.94 9.27 -5.99 -25.75 -26.70 -24.01 -10.01 -6.21 -24.17 -27.38 -24.62 -7.15
Peroxisomes -2.18 0.47 -1.06 0.64 -1.27 0.77 5.99 4.10 3.95 -0.78 0.08 5.43 3.16 3.58 -1.45
Vacuole 7.68 2.12 -7.10 -16.42 -8.65 -11.96 -30.49 -27.23 -20.74 -8.84 -2.41 -18.04 -25.54 -26.19 -13.03
Vacuole Periphery 0.38 4.21 5.87 10.25 2.08 2.55 5.26 8.34 9.12 5.29 2.01 6.10 10.40 13.08 7.15
Standard name
Human Ortholog
Description Mitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress
Localization
Cell Percentages mitochondrion (62%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mdl1

Mdl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available