Standard name | |
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Human Ortholog | |
Description | Mitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 9 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 7 | 2 | 0 | 0 | 1 | 6 | 10 | 11 | 18 | 28 | 31 |
Bud | 2 | 14 | 7 | 10 | 3 | 8 | 22 | 28 | – | 31 | 13 | 28 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 6 | 2 | 7 | 17 | 16 |
Bud Neck | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 2 | – | 1 | 0 | 1 | 3 | 4 | 1 | 0 | 0 | 0 | 1 | 9 | 4 | 8 | 9 | 21 |
Bud Site | 1 | 2 | 1 | 0 | 1 | 1 | 13 | 12 | – | 22 | 8 | 14 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 3 | 0 | 1 | 2 | 5 | 2 | – | 2 | 5 | 8 | 3 | 0 | 2 | 2 | 1 | 1 | 0 | 2 | 0 | 3 | 5 | 6 |
Cytoplasm | 94 | 140 | 101 | 68 | 82 | 57 | 87 | 76 | – | 47 | 16 | 26 | 179 | 191 | 210 | 41 | 49 | 4 | 53 | 73 | 38 | 76 | 103 | 136 |
Endoplasmic Reticulum | 1 | 23 | 2 | 1 | 1 | 3 | 1 | 1 | – | 2 | 1 | 0 | 2 | 5 | 5 | 3 | 6 | 0 | 3 | 2 | 2 | 5 | 4 | 5 |
Endosome | 9 | 0 | 16 | 8 | 35 | 11 | 11 | 13 | – | 0 | 1 | 2 | 12 | 6 | 18 | 21 | 23 | 8 | 11 | 30 | 39 | 38 | 40 | 44 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 0 | 2 | 3 | 5 | 7 | 6 | 7 | 6 |
Mitochondria | 65 | 237 | 193 | 125 | 193 | 365 | 510 | 676 | – | 649 | 471 | 642 | 15 | 12 | 1 | 15 | 14 | 1 | 121 | 232 | 219 | 253 | 287 | 274 |
Nucleus | 0 | 1 | 0 | 0 | 3 | 4 | 11 | 19 | – | 5 | 6 | 11 | 1 | 1 | 2 | 5 | 7 | 0 | 0 | 0 | 0 | 1 | 2 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 5 | 5 | 16 | 17 | – | 21 | 18 | 27 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 3 | 4 |
Nucleolus | 0 | 1 | 0 | 1 | 3 | 3 | 17 | 32 | – | 33 | 9 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 3 |
Peroxisomes | 5 | 0 | 26 | 9 | 20 | 6 | 15 | 28 | – | 6 | 2 | 12 | 5 | 0 | 4 | 4 | 10 | 4 | 5 | 5 | 21 | 22 | 31 | 25 |
SpindlePole | 5 | 6 | 24 | 8 | 22 | 8 | 53 | 58 | – | 34 | 17 | 46 | 6 | 2 | 2 | 7 | 7 | 3 | 8 | 15 | 12 | 21 | 38 | 40 |
Vac/Vac Membrane | 10 | 14 | 13 | 3 | 35 | 30 | 28 | 17 | – | 20 | 12 | 23 | 12 | 8 | 17 | 26 | 35 | 5 | 4 | 18 | 3 | 13 | 17 | 22 |
Unique Cell Count | 177 | 382 | 326 | 200 | 335 | 420 | 633 | 789 | 699 | 499 | 689 | 231 | 234 | 256 | 109 | 120 | 20 | 233 | 429 | 372 | 493 | 617 | 654 | |
Labelled Cell Count | 196 | 452 | 389 | 234 | 406 | 503 | 789 | 981 | 873 | 579 | 859 | 243 | 239 | 266 | 126 | 152 | 29 | 233 | 429 | 372 | 493 | 617 | 654 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 3.8 | 3.9 | 3.9 | 4.1 | 3.6 | 3.5 | 3.8 | – | 3.4 | 3.7 | 3.8 | 5.2 | 5.3 | 5.5 | 5.9 | 6.8 | 8.2 | 5.4 | 5.6 | 5.6 |
Std Deviation (1e-4) | 1.0 | 0.6 | 1.1 | 1.3 | 1.5 | 1.2 | 1.2 | 1.2 | – | 1.0 | 1.1 | 1.1 | 1.1 | 1.0 | 1.1 | 1.6 | 1.9 | 1.4 | 1.4 | 1.7 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.08 | -0.11 | -0.15 | -0.02 | – | -0.18 | -0.06 | -0.03 | 0.44 | 0.46 | 0.52 | 0.62 | 0.82 | 1.09 | 0.48 | 0.54 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.3 | 7.6 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0086 | 0.0132 | 0.0386 | 0.0057 | 0.0068 | 0.0346 | 0.0577 | 0.0062 | 0.0277 | 0.0743 | 0.0222 | 0.0202 | – | – | – | – | – | – |
Bud | 0.001 | 0.0031 | 0.0045 | 0.002 | 0.0031 | 0.0041 | 0.0014 | 0.0023 | 0.002 | 0.0048 | 0.0004 | 0.0015 | – | – | – | – | – | – |
Bud Neck | 0.002 | 0.002 | 0.0009 | 0.0033 | 0.0012 | 0.0063 | 0.0043 | 0.0016 | 0.0033 | 0.0006 | 0.0003 | 0.0264 | – | – | – | – | – | – |
Bud Periphery | 0.0017 | 0.0046 | 0.0197 | 0.0059 | 0.009 | 0.011 | 0.003 | 0.004 | 0.0059 | 0.0085 | 0.0004 | 0.0036 | – | – | – | – | – | – |
Bud Site | 0.0141 | 0.0493 | 0.013 | 0.0088 | 0.0215 | 0.0033 | 0.0219 | 0.0301 | 0.0131 | 0.0047 | 0.0006 | 0.0023 | – | – | – | – | – | – |
Cell Periphery | 0.0009 | 0.0013 | 0.001 | 0.0044 | 0.0006 | 0.0011 | 0.0039 | 0.0022 | 0.0028 | 0.0004 | 0.0003 | 0.0013 | – | – | – | – | – | – |
Cytoplasm | 0.0344 | 0.0592 | 0.0577 | 0.0427 | 0.0383 | 0.0539 | 0.0669 | 0.1376 | 0.0546 | 0.0419 | 0.026 | 0.0887 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.1062 | 0.0814 | 0.0633 | 0.0901 | 0.0942 | 0.1038 | 0.175 | 0.1178 | 0.0948 | 0.0627 | 0.0779 | 0.1008 | – | – | – | – | – | – |
Eisosomes | 0.0008 | 0.0009 | 0.0007 | 0.0013 | 0.0004 | 0.0008 | 0.0014 | 0.0007 | 0.0015 | 0.0002 | 0.0009 | 0.0006 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0055 | 0.0056 | 0.0064 | 0.0038 | 0.0024 | 0.0054 | 0.004 | 0.0058 | 0.003 | 0.0036 | 0.0005 | 0.0052 | – | – | – | – | – | – |
Endosome | 0.1419 | 0.1101 | 0.1298 | 0.0907 | 0.1461 | 0.1003 | 0.1016 | 0.0802 | 0.1013 | 0.1101 | 0.0691 | 0.0676 | – | – | – | – | – | – |
Golgi | 0.039 | 0.0624 | 0.0563 | 0.0364 | 0.0666 | 0.0624 | 0.0347 | 0.0315 | 0.0541 | 0.0246 | 0.0409 | 0.0529 | – | – | – | – | – | – |
Lipid Particles | 0.091 | 0.0211 | 0.0221 | 0.0412 | 0.0266 | 0.0507 | 0.0459 | 0.0302 | 0.025 | 0.0506 | 0.0313 | 0.0131 | – | – | – | – | – | – |
Mitochondria | 0.2339 | 0.2938 | 0.3727 | 0.4286 | 0.3662 | 0.2937 | 0.1076 | 0.1508 | 0.2611 | 0.27 | 0.1594 | 0.2641 | – | – | – | – | – | – |
None | 0.2685 | 0.2301 | 0.155 | 0.1418 | 0.1331 | 0.1925 | 0.2608 | 0.2701 | 0.2756 | 0.2327 | 0.5313 | 0.2882 | – | – | – | – | – | – |
Nuclear Periphery | 0.0038 | 0.0016 | 0.0026 | 0.0017 | 0.0007 | 0.0023 | 0.0025 | 0.001 | 0.0009 | 0.0015 | 0.0001 | 0.0009 | – | – | – | – | – | – |
Nucleolus | 0.0009 | 0.0013 | 0.0005 | 0.009 | 0.0003 | 0.0019 | 0.004 | 0.0013 | 0.0014 | 0.0004 | 0.0001 | 0.0008 | – | – | – | – | – | – |
Nucleus | 0.0011 | 0.0017 | 0.0012 | 0.0046 | 0.0006 | 0.0036 | 0.0027 | 0.0021 | 0.0008 | 0.0007 | 0.0002 | 0.001 | – | – | – | – | – | – |
Peroxisomes | 0.0268 | 0.0394 | 0.0255 | 0.0594 | 0.0659 | 0.0511 | 0.0791 | 0.0896 | 0.0588 | 0.0854 | 0.035 | 0.052 | – | – | – | – | – | – |
Punctate Nuclear | 0.0043 | 0.0023 | 0.0014 | 0.0013 | 0.001 | 0.005 | 0.0113 | 0.0012 | 0.0009 | 0.0007 | 0.0004 | 0.0012 | – | – | – | – | – | – |
Vacuole | 0.0084 | 0.0117 | 0.0156 | 0.0137 | 0.0096 | 0.0086 | 0.0079 | 0.0266 | 0.0088 | 0.0129 | 0.0017 | 0.0053 | – | – | – | – | – | – |
Vacuole Periphery | 0.0052 | 0.0041 | 0.0112 | 0.0034 | 0.0056 | 0.0039 | 0.0024 | 0.0071 | 0.0027 | 0.0087 | 0.0009 | 0.0024 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.5769 | 33.5406 | 22.7853 | 27.299 | 33.9154 | 25.3516 | 28.7781 | 27.5634 | 31.1316 | 40.1197 |
Translational Efficiency | 0.4514 | 0.3731 | 0.4667 | 0.4251 | 0.422 | 0.658 | 0.3603 | 0.3515 | 0.3363 | 0.3498 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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