Standard name
Human Ortholog
Description Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0.05 0.05 0 0.05 0 0 0 0 0 0 0 0 0.66 0.68 0.67 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0.09 0 0 0 0 0 0
Endosome 0.13 0 0.08 0.16 0.16 0 0.16 0.18 0.06 0 0.05 0.05 0.08 0.06 0.1 0.15 0.09 0.09 0.07 0 0.06 0.06 0.09 0.07
Golgi 0.06 0.09 0.26 0 0.13 0 0 0 0 0 0 0 0.11 0.18 0.19 0 0 0 0.08 0.11 0 0.09 0.09 0.09
Mitochondria 0.78 0.89 0.55 0.83 0.64 0.89 0.69 0.73 0.87 0.84 0.92 0.87 0.81 0.77 0.72 0.09 0.1 0.07 0.71 0.67 0.82 0.69 0.65 0.58
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.18 0.08 0 0 0.05 0 0 0 0 0 0.07 0 0 0 0 0.05 0 0 0.07 0.07 0.06
SpindlePole 0 0 0.06 0.07 0 0 0.06 0.06 0 0 0 0.05 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.15 0.11 0.05 0.14 0.12 0.17 0.19 0.1 0.1 0.07 0.08 0.13 0.13 0.13 0.1 0.08 0.1 0 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 5 7 2 2 2 0 0 0 1 0 2 2 4 1 3 5 17 4 5 1 2 14 20
Bud 1 0 4 5 6 7 7 20 9 8 9 6 4 4 3 4 4 8 0 1 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 0 2 3 6
Bud Site 0 0 0 0 1 0 0 1 4 1 2 4 0 0 0 1 0 0
Cell Periphery 8 6 3 11 13 3 1 4 2 2 1 3 10 13 6 5 10 5 0 0 0 0 1 0
Cytoplasm 0 6 6 11 14 9 17 19 6 12 5 7 5 9 10 206 213 215 0 0 0 0 0 2
Endoplasmic Reticulum 3 2 6 0 1 7 0 0 2 2 1 3 7 2 16 19 26 28 1 1 0 0 3 2
Endosome 35 4 8 38 45 4 56 94 17 15 13 16 16 21 34 45 28 28 21 6 8 15 37 27
Golgi 17 12 27 10 38 0 5 5 0 5 1 6 22 62 62 1 2 2 23 15 6 22 41 35
Mitochondria 217 116 58 194 185 230 237 384 261 308 251 269 164 260 239 29 31 22 217 98 128 174 283 218
Nucleus 1 0 1 2 3 4 3 13 5 8 2 2 0 2 3 2 2 6 0 0 0 0 2 0
Nuclear Periphery 9 3 0 0 1 9 7 19 4 13 7 17 5 1 3 0 0 0 3 1 0 1 1 1
Nucleolus 5 0 1 1 1 9 12 20 15 9 9 12 3 6 0 0 0 1 0 0 0 1 2 1
Peroxisomes 6 4 3 42 22 2 8 26 9 9 1 7 2 22 15 9 7 0 14 5 2 18 28 22
SpindlePole 3 0 6 16 11 5 22 31 12 8 4 14 2 1 1 18 15 10 3 0 0 0 3 7
Vac/Vac Membrane 54 19 12 11 40 30 57 102 29 35 18 25 26 43 42 30 26 32 12 6 4 8 9 22
Unique Cell Count 278 130 105 235 288 257 343 528 299 366 272 308 203 338 334 310 315 321 309 146 157 251 438 376
Labelled Cell Count 360 177 142 343 383 321 432 738 375 436 324 393 268 450 435 374 370 374 309 146 157 251 438 376


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.1 7.0 6.9 7.6 5.0 6.6 6.4 6.0 6.1 5.7 6.2 6.9 7.1 7.2 5.3 5.5 5.4 5.9 5.9 6.1
Std Deviation (1e-4) 0.9 2.1 1.3 1.7 1.8 1.2 1.6 1.4 1.5 1.6 1.5 1.6 1.2 1.2 1.1 1.0 0.9 1.0 1.2 1.0 1.7
Intensity Change (Log2) -0.02 0.12 -0.48 -0.09 -0.13 -0.23 -0.2 -0.29 -0.18 -0.02 0.02 0.04 -0.4 -0.34 -0.37 -0.26 -0.25 -0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.5
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -0.3 0 -0.3 0 0 0 0 0 0 0 0 10.8 11.0 10.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 -0.4 0.2 0.8 1.0
Endosome 2.1 2.1 0 2.2 2.6 -0.7 -1.5 -1.1 -0.9 0.1 -0.5 0.8 1.8 0.4 0.4
Golgi -5.9 -3.0 -8.5 -8.4 -10.6 -9.1 -8.7 -8.4 -7.8 -3.4 -1.6 -1.6 -9.0 -8.8 -8.9
Mitochondria 5.3 1.6 7.3 2.6 3.6 6.9 6.3 8.4 7.0 4.7 4.3 3.1 -10.0 -9.9 -11.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 3.8 1.7 0 0 0 0 0 0 0 0 1.4 0 0 0 0
SpindlePole 0.4 0 0 0.3 0.1 0 0 0 -0.5 0 0 0 0 -0.4 0
Vacuole -2.3 0.6 0.1 1.3 1.9 -0.5 -0.6 -1.5 -1.0 0.3 0.4 0.3 -0.5 -1.0 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1512 2.4559 2.3953 2.1524 2.4542 2.4545 3.5777 4.0503 4.2322 3.651 3.7653 3.9292 5.0281 5.909 5.6455 5.6252 5.1337 5.863
Actin 0.0218 0.0136 0.0023 0.0002 0.0151 0.0015 0.0117 0.0049 0.0117 0.0006 0.0034 0.0036 0.0093 0.0278 0.04 0.0495 0.0067 0.0102
Bud 0.0005 0.0005 0.0002 0 0.0005 0.0002 0.0006 0.0014 0.0003 0.0003 0.0009 0.0002 0.0008 0.002 0.0012 0.0018 0.0032 0.0019
Bud Neck 0.0003 0.0002 0.0019 0.0001 0.0002 0.0003 0.0013 0.0007 0.0002 0.0002 0.0006 0.0004 0.0008 0.001 0.0004 0.0004 0.0002 0.0014
Bud Periphery 0.0013 0.001 0.0004 0.0001 0.0009 0.0004 0.0012 0.0034 0.0005 0.0008 0.0017 0.0005 0.0013 0.0027 0.0035 0.0029 0.0062 0.007
Bud Site 0.0012 0.0014 0.0005 0.0001 0.0003 0.0002 0.0053 0.0105 0.001 0.0003 0.0011 0.0002 0.0097 0.0163 0.0047 0.0048 0.001 0.0004
Cell Periphery 0.0004 0.0002 0.0002 0 0.0002 0.0001 0.0005 0.0006 0.0002 0.0001 0.0002 0.0001 0.0008 0.001 0.0006 0.0003 0.0002 0.0007
Cytoplasm 0.0007 0.0029 0.0003 0 0.0007 0.0039 0.0009 0.0011 0.0004 0.0002 0.0015 0.0001 0.0005 0.0065 0.0021 0.0005 0.0025 0.0104
Cytoplasmic Foci 0.0175 0.0146 0.0074 0.0034 0.0051 0.017 0.0186 0.0271 0.0068 0.0061 0.021 0.0032 0.0196 0.0359 0.0156 0.0067 0.011 0.0081
Eisosomes 0.0007 0.0002 0.0001 0.0001 0.0002 0.0001 0.0003 0.0003 0.0004 0.0001 0.0001 0.0003 0.0003 0.0004 0.001 0.0005 0.0002 0.0003
Endoplasmic Reticulum 0.0005 0.0014 0.0001 0 0.0002 0.0002 0.0023 0.0009 0.0005 0.0002 0.0007 0.0003 0.0003 0.0014 0.001 0.0005 0.0005 0.0003
Endosome 0.035 0.041 0.0301 0.0138 0.0181 0.0226 0.0583 0.0545 0.0257 0.059 0.1 0.025 0.0392 0.0909 0.0433 0.0671 0.0712 0.0196
Golgi 0.0467 0.0371 0.0447 0.016 0.0448 0.0411 0.0652 0.0702 0.0463 0.0505 0.0682 0.0299 0.0578 0.0782 0.0642 0.0176 0.0651 0.0396
Lipid Particles 0.015 0.0107 0.0036 0.0028 0.0128 0.0082 0.0317 0.0124 0.0088 0.022 0.0052 0.0038 0.0329 0.0067 0.0076 0.0029 0.0088 0.0017
Mitochondria 0.8207 0.8433 0.8916 0.9551 0.8851 0.8664 0.7535 0.7845 0.8811 0.8483 0.7663 0.9095 0.7704 0.6694 0.7815 0.7787 0.7588 0.8776
None 0.0013 0.0002 0.0001 0 0.0001 0.0018 0.0007 0.0002 0.0011 0.0001 0.0003 0.0001 0.0005 0.0012 0.001 0.0002 0.0003 0.005
Nuclear Periphery 0.0005 0.0014 0.0001 0 0 0.0001 0.0019 0.0003 0.0016 0.0002 0.0007 0.0004 0.0002 0.0004 0.0013 0.0002 0.0003 0.0001
Nucleolus 0.0003 0.0002 0.0002 0 0.0001 0.0004 0.0021 0.0003 0.0002 0.0002 0.0002 0.0001 0.0004 0.0012 0.0002 0.0001 0.0001 0.0001
Nucleus 0.0003 0.0002 0.0001 0 0.0001 0.0002 0.0023 0.0003 0.0002 0.0001 0.0003 0.0001 0.0002 0.0007 0.0002 0.0001 0.0002 0.0001
Peroxisomes 0.023 0.0185 0.008 0.0069 0.0114 0.0311 0.0208 0.0179 0.0079 0.0042 0.0127 0.0087 0.0478 0.0334 0.0255 0.0595 0.0499 0.0128
Punctate Nuclear 0.0026 0.0001 0 0 0 0.0001 0.0014 0.0001 0.0005 0.0001 0.0001 0 0.0008 0.0002 0.0002 0.0004 0.0001 0
Vacuole 0.0049 0.0045 0.0043 0.0004 0.0019 0.0018 0.0111 0.0048 0.002 0.0032 0.0072 0.004 0.0033 0.0154 0.0029 0.0034 0.0074 0.0012
Vacuole Periphery 0.0049 0.0068 0.0039 0.0007 0.0022 0.0022 0.0082 0.0036 0.0027 0.0032 0.0078 0.0095 0.0033 0.0072 0.0021 0.0022 0.0061 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.0325 24.2858 22.1798 25.1583 23.0307 27.1521 30.2219 28.6844 26.9514 28.9979
Translational Efficiency 1.6239 1.1538 1.1706 1.1024 1.5269 1.3633 1.4478 1.2468 1.3452 1.4501

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency