Standard name
Description Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.96 0.99 0.98 0.97 0.93 0.94 0.9 0.81 0.78 0.75 0.75 1.0 0.99 0.98 0.92 0.97 0.95 0.87 0.85 0.8 0.82 0.82 0.79
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0.19 0.08 0.14 0.32 0.29 0.36 0.38 0 0 0 0 0 0 0.05 0.05 0.09 0.05 0.06 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 4 7 2 4 5
Bud – 2 0 1 0 7 1 7 7 15 8 11 0 0 0 1 0 1 0 5 5 3 9 14
Bud Neck – 1 13 6 9 10 10 10 6 7 6 1 1 0 0 0 0 1 0 0 1 0 1 5
Bud Site – 0 0 1 0 1 0 0 0 0 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery – 6 7 8 2 1 6 6 6 5 2 6 1 3 0 5 4 4 0 2 10 1 2 3
Cytoplasm – 363 548 401 434 306 480 574 289 282 233 262 226 329 322 396 365 312 47 308 425 198 313 308
Endoplasmic Reticulum – 1 0 2 0 0 0 0 1 0 0 0 1 3 2 11 13 12 0 0 1 1 1 2
Endosome – 1 0 2 0 0 1 0 0 2 0 1 1 0 0 1 0 0 0 7 6 2 2 3
Golgi – 1 1 0 0 0 0 0 0 0 0 0 0 1 2 0 1 0 1 3 1 1 1 2
Mitochondria – 15 1 8 7 63 41 89 113 104 113 131 2 2 0 9 0 6 2 16 46 11 23 13
Nucleus – 1 1 0 2 5 0 5 3 1 2 2 0 0 1 9 5 10 0 2 0 0 0 1
Nuclear Periphery – 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0
Nucleolus – 0 1 0 0 1 0 2 0 0 1 3 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 1 0 1 2 0 1 0 3 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole – 0 7 4 10 0 17 13 12 18 12 18 0 1 0 0 0 0 0 2 6 3 7 8
Vac/Vac Membrane – 1 1 0 4 0 3 6 4 4 3 3 0 0 0 41 17 16 0 3 5 4 3 8
Unique Cell Count 379 556 408 449 330 509 639 357 363 311 349 227 334 327 429 378 329 55 363 532 242 382 390
Labelled Cell Count 394 580 434 469 394 560 715 441 439 381 444 232 339 328 475 405 362 55 363 532 242 382 390


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.1 4.9 4.3 5.2 3.4 4.4 4.1 3.9 3.9 3.7 3.8 4.5 4.5 4.8 7.6 8.3 7.2 4.1 3.9 4.4
Std Deviation (1e-4) – 1.4 1.3 1.0 1.7 1.5 2.4 1.3 1.7 1.4 1.4 1.2 1.5 1.2 1.6 1.9 1.8 1.6 1.7 1.4 1.9
Intensity Change (Log2) – – – -0.19 0.08 -0.53 -0.18 -0.27 -0.36 -0.35 -0.4 -0.39 -0.15 -0.12 -0.03 0.62 0.76 0.54 -0.27 -0.32 -0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.2 4.7 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4381 -0.1602 -0.2639 -0.8465 -0.3141 -0.1792 0.731 1.1027 1.0126 0.6164 0.4894 1.0987 3.2015 3.9237 3.1479 2.9582 3.3134 3.4664
Actin 0.0684 0.0003 0.0127 0.0069 0.0043 0.0015 0.0001 0.0004 0.0014 0.0002 0.0002 0.0003 0.007 0.0012 0.0013 0.0008 0.0003 0.0004
Bud 0.0099 0.0025 0.0004 0.0043 0.001 0.004 0.0001 0.0022 0.0018 0.0002 0.0008 0.0003 0.001 0.0004 0.0005 0.0003 0.0001 0.0001
Bud Neck 0.0065 0.0003 0.0016 0.0005 0.0009 0.0009 0.0001 0.0003 0.0002 0.0001 0.0001 0.0005 0.0017 0.0006 0.0003 0.0024 0.0001 0.0022
Bud Periphery 0.0031 0.0005 0.0009 0.0014 0.0013 0.0021 0.0001 0.0008 0.0018 0.0002 0.0025 0.0004 0.0018 0.0003 0.0004 0.0004 0.0002 0.0001
Bud Site 0.0062 0.0078 0.0008 0.0027 0.0015 0.0003 0.0003 0.0035 0.0009 0.0001 0.0001 0.0001 0.0008 0.0076 0.0016 0.0002 0.0001 0.0004
Cell Periphery 0.0008 0.0003 0.0001 0.0004 0.0002 0.0001 0 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.1481 0.2168 0.2042 0.1231 0.1901 0.2925 0.2743 0.2461 0.2321 0.1993 0.2066 0.3084 0.2678 0.256 0.2549 0.1663 0.1922 0.3794
Cytoplasmic Foci 0.0196 0.0107 0.0075 0.0133 0.0147 0.0086 0.005 0.0152 0.0068 0.0047 0.0073 0.0079 0.0096 0.0102 0.0035 0.0089 0.0059 0.0057
Eisosomes 0.0009 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0066 0.0018 0.0037 0.0015 0.0032 0.0022 0.0009 0.0009 0.0008 0.0007 0.0008 0.0007 0.0022 0.0006 0.0009 0.0007 0.0006 0.0008
Endosome 0.015 0.0072 0.0033 0.0049 0.0117 0.0035 0.0009 0.0027 0.0021 0.0013 0.0019 0.0013 0.0028 0.001 0.0007 0.0012 0.001 0.001
Golgi 0.0056 0.0014 0.0004 0.0004 0.0015 0.0005 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0005 0.0002 0.0001 0.0002 0.0001 0.0001
Lipid Particles 0.0101 0.0003 0.0007 0.0004 0.0022 0.0014 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0009 0.0001 0.0001
Mitochondria 0.0035 0.0008 0.0005 0.0008 0.0025 0.001 0.0003 0.0006 0.0014 0.0003 0.0007 0.0002 0.0008 0.0002 0.0002 0.0003 0.0002 0.0001
None 0.6415 0.7293 0.748 0.8244 0.7261 0.6639 0.7135 0.7098 0.7404 0.7862 0.7722 0.6719 0.6941 0.7117 0.732 0.813 0.7951 0.6054
Nuclear Periphery 0.0091 0.0012 0.0014 0.0011 0.0118 0.0021 0.0004 0.0007 0.0007 0.0009 0.0004 0.0006 0.0019 0.0003 0.0005 0.0004 0.0004 0.0003
Nucleolus 0.0063 0.0007 0.0006 0.0019 0.0021 0.0004 0.0002 0.0006 0.0003 0.0001 0.0002 0.0001 0.0002 0.0005 0.0001 0.0002 0.0001 0.0001
Nucleus 0.0127 0.0092 0.0029 0.0041 0.012 0.0066 0.0019 0.0052 0.0036 0.0035 0.002 0.004 0.0022 0.0034 0.0011 0.0008 0.0011 0.0023
Peroxisomes 0.013 0.0002 0.0005 0.0025 0.0008 0.001 0.0001 0.0043 0.0022 0.0001 0.0001 0.0002 0.0025 0.0009 0.0001 0.0015 0.0002 0.0001
Punctate Nuclear 0.0083 0.0068 0.0087 0.004 0.0081 0.0064 0.001 0.0046 0.002 0.001 0.0008 0.0025 0.0013 0.0037 0.0009 0.0008 0.0016 0.001
Vacuole 0.0037 0.0016 0.0008 0.0009 0.0027 0.0008 0.0005 0.0013 0.0008 0.0007 0.0025 0.0004 0.0008 0.0007 0.0005 0.0005 0.0005 0.0003
Vacuole Periphery 0.001 0.0002 0.0002 0.0002 0.0013 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.5809 21.3655 24.662 30.8965 23.241 25.2143 24.6031 26.7307 30.2319 26.6134
Translational Efficiency 2.2148 1.7063 1.8502 1.2518 1.569 2.2027 2.0818 1.9244 1.7085 1.7433

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘