Standard name | |
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Description | Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 2 | 1 | 1 | 4 | 4 | 2 | 0 | 3 | 1 | 4 | 5 | 11 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 2 | 3 | 3 | 2 | 4 | 1 | 9 | 4 | 9 | 8 | 10 | 3 | 8 | 5 | 9 | 7 | 4 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 125 | 148 | 121 | 94 | 111 | 126 | 158 | 165 | 65 | 105 | 81 | 81 | 180 | 292 | 245 | 372 | 270 | 223 | 117 | 148 | 108 | 92 | 192 | 174 |
Endoplasmic Reticulum | 1 | 0 | 1 | 1 | 2 | 0 | 5 | 13 | 8 | 9 | 5 | 5 | 0 | 3 | 3 | 12 | 7 | 5 | 0 | 1 | 1 | 1 | 2 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 5 | 5 | 6 | 4 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 4 | 1 | 6 | 1 | 6 | 0 | 1 | 2 | 8 | 2 | 0 | 2 | 1 | 2 | 2 | 3 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 0 | 0 | 7 | 3 | 3 | 8 | 16 | 1 | 1 | 3 | 0 | 1 | 6 | 1 | 14 | 2 | 1 | 0 | 0 | 7 | 2 | 3 | 8 |
Unique Cell Count | 129 | 148 | 122 | 100 | 118 | 132 | 176 | 199 | 77 | 125 | 97 | 96 | 180 | 300 | 257 | 392 | 275 | 226 | 123 | 157 | 126 | 110 | 213 | 207 |
Labelled Cell Count | 134 | 150 | 128 | 107 | 122 | 136 | 181 | 218 | 89 | 137 | 105 | 108 | 188 | 315 | 267 | 418 | 289 | 233 | 123 | 157 | 126 | 110 | 213 | 207 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 25.2 | 22.5 | 22.6 | 22.4 | 26.2 | 27.1 | 25.7 | 24.4 | 25.0 | 21.8 | 24.7 | 24.3 | 32.6 | 38.4 | 39.3 | 36.7 | 47.1 | 48.2 | 18.7 | 23.6 | 29.3 |
Std Deviation (1e-4) | 7.4 | 6.0 | 6.1 | 5.5 | 5.9 | 5.8 | 6.7 | 6.4 | 8.5 | 6.8 | 6.6 | 5.5 | 11.6 | 12.9 | 14.1 | 9.9 | 13.6 | 17.2 | 7.3 | 7.6 | 12.3 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.22 | 0.26 | 0.19 | 0.11 | 0.14 | -0.05 | 0.13 | 0.11 | 0.53 | 0.77 | 0.8 | 0.7 | 1.06 | 1.09 | -0.27 | 0.06 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.2 | -2.2 | -1.8 | -3.3 | -4.5 | -4.1 | -4.3 | -4.3 | -4.2 | 1.2 | -1.2 | -1.9 | -2.1 | -0.8 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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