Standard name
Description Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.05 0.05 0.07 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.99 0.94 0.94 0.95 0.9 0.83 0.84 0.84 0.84 0.84 1.0 0.97 0.95 0.95 0.98 0.99 0.95 0.95 0.86 0.84 0.9 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0.1 0.07 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 3 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 2 2 1 1 4 4 2 0 3 1 4 5 11 0 1 0 0 1 0 0 1 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 2 3 3 2 4 1 9 4 9 8 10 3 8 5 9 7 4 0 0 1 0 0 0
Cytoplasm 125 148 121 94 111 126 158 165 65 105 81 81 180 292 245 372 270 223 117 148 108 92 192 174
Endoplasmic Reticulum 1 0 1 1 2 0 5 13 8 9 5 5 0 3 3 12 7 5 0 1 1 1 2 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0
Golgi 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3 0 4 5 5 6 4 2 0 0 0 2 0 0 0 0 1 2 0 2
Nucleus 0 0 1 0 0 2 0 4 1 6 1 6 0 1 2 8 2 0 2 1 2 2 3 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 0 7 3 3 8 16 1 1 3 0 1 6 1 14 2 1 0 0 7 2 3 8
Unique Cell Count 129 148 122 100 118 132 176 199 77 125 97 96 180 300 257 392 275 226 123 157 126 110 213 207
Labelled Cell Count 134 150 128 107 122 136 181 218 89 137 105 108 188 315 267 418 289 233 123 157 126 110 213 207


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.2 22.5 22.6 22.4 26.2 27.1 25.7 24.4 25.0 21.8 24.7 24.3 32.6 38.4 39.3 36.7 47.1 48.2 18.7 23.6 29.3
Std Deviation (1e-4) 7.4 6.0 6.1 5.5 5.9 5.8 6.7 6.4 8.5 6.8 6.6 5.5 11.6 12.9 14.1 9.9 13.6 17.2 7.3 7.6 12.3
Intensity Change (Log2) – – – -0.01 0.22 0.26 0.19 0.11 0.14 -0.05 0.13 0.11 0.53 0.77 0.8 0.7 1.06 1.09 -0.27 0.06 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1.7 0 2.5 0 0 0 0 0 0
Cytoplasm -2.2 -2.2 -1.8 -3.3 -4.5 -4.1 -4.3 -4.3 -4.2 1.2 -1.2 -1.9 -2.1 -0.8 -0.4
Endoplasmic Reticulum 0 0 0 0 2.4 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.2 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tub4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available