Standard name | |
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Human Ortholog | |
Description | Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 2 | 1 | 1 | 4 | 4 | 2 | 0 | 3 | 1 | 4 | 5 | 11 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 2 | 3 | 3 | 2 | 4 | 1 | 9 | 4 | 9 | 8 | 10 | 3 | 8 | 5 | 9 | 7 | 4 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 125 | 148 | 121 | 94 | 111 | 126 | 158 | 165 | 65 | 105 | 81 | 81 | 180 | 292 | 245 | 372 | 270 | 223 | 117 | 148 | 108 | 92 | 192 | 174 |
Endoplasmic Reticulum | 1 | 0 | 1 | 1 | 2 | 0 | 5 | 13 | 8 | 9 | 5 | 5 | 0 | 3 | 3 | 12 | 7 | 5 | 0 | 1 | 1 | 1 | 2 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 5 | 5 | 6 | 4 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 4 | 1 | 6 | 1 | 6 | 0 | 1 | 2 | 8 | 2 | 0 | 2 | 1 | 2 | 2 | 3 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 0 | 0 | 7 | 3 | 3 | 8 | 16 | 1 | 1 | 3 | 0 | 1 | 6 | 1 | 14 | 2 | 1 | 0 | 0 | 7 | 2 | 3 | 8 |
Unique Cell Count | 129 | 148 | 122 | 100 | 118 | 132 | 176 | 199 | 77 | 125 | 97 | 96 | 180 | 300 | 257 | 392 | 275 | 226 | 123 | 157 | 126 | 110 | 213 | 207 |
Labelled Cell Count | 134 | 150 | 128 | 107 | 122 | 136 | 181 | 218 | 89 | 137 | 105 | 108 | 188 | 315 | 267 | 418 | 289 | 233 | 123 | 157 | 126 | 110 | 213 | 207 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 25.2 | 22.5 | 22.6 | 22.4 | 26.2 | 27.1 | 25.7 | 24.4 | 25.0 | 21.8 | 24.7 | 24.3 | 32.6 | 38.4 | 39.3 | 36.7 | 47.1 | 48.2 | 18.7 | 23.6 | 29.3 |
Std Deviation (1e-4) | 7.4 | 6.0 | 6.1 | 5.5 | 5.9 | 5.8 | 6.7 | 6.4 | 8.5 | 6.8 | 6.6 | 5.5 | 11.6 | 12.9 | 14.1 | 9.9 | 13.6 | 17.2 | 7.3 | 7.6 | 12.3 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.22 | 0.26 | 0.19 | 0.11 | 0.14 | -0.05 | 0.13 | 0.11 | 0.53 | 0.77 | 0.8 | 0.7 | 1.06 | 1.09 | -0.27 | 0.06 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.2 | -2.2 | -1.8 | -3.3 | -4.5 | -4.1 | -4.3 | -4.3 | -4.2 | 1.2 | -1.2 | -1.9 | -2.1 | -0.8 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.4823 | 20.2919 | 19.6388 | 17.51 | 16.9083 | 17.9331 | 17.6971 | 23.9426 | 21.7452 | 19.3114 | 17.558 | 19.3791 | 14.8495 | 21.4918 | 20.7739 | 17.8628 | 19.8526 | 18.7459 |
Actin | 0.0141 | 0.0005 | 0.0002 | 0.0002 | 0.0029 | 0.0012 | 0.0178 | 0.0004 | 0.0052 | 0.002 | 0.015 | 0.0022 | 0.0586 | 0.0002 | 0.0076 | 0.0932 | 0.0042 | 0.0023 |
Bud | 0.0003 | 0.0025 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0006 | 0.0005 | 0.0021 | 0.0016 | 0.0001 | 0.0003 | 0.001 | 0.0001 | 0.0016 |
Bud Neck | 0.0006 | 0.0002 | 0.0002 | 0.0004 | 0.0006 | 0.0009 | 0.001 | 0.0002 | 0.0007 | 0.0007 | 0.0007 | 0.0011 | 0.0151 | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.0008 |
Bud Periphery | 0.0002 | 0.0013 | 0 | 0 | 0.0011 | 0 | 0.0004 | 0 | 0.0003 | 0.0004 | 0.0003 | 0.0026 | 0.0019 | 0 | 0.0004 | 0.0013 | 0 | 0.0004 |
Bud Site | 0.0003 | 0.0014 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0043 | 0.0003 | 0.0117 | 0.0056 | 0.0008 | 0.0002 | 0.0352 | 0.0001 | 0.001 | 0.0015 | 0.0001 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0002 | 0 | 0 | 0.0001 |
Cytoplasm | 0.8825 | 0.9806 | 0.9764 | 0.9742 | 0.9595 | 0.9116 | 0.8583 | 0.9865 | 0.9646 | 0.87 | 0.8253 | 0.9327 | 0.6822 | 0.9897 | 0.8789 | 0.8756 | 0.9027 | 0.9022 |
Cytoplasmic Foci | 0.0132 | 0.0004 | 0.0006 | 0.0004 | 0.001 | 0.0025 | 0.0226 | 0.001 | 0.0013 | 0.0152 | 0.0136 | 0.0072 | 0.0238 | 0.0006 | 0.0107 | 0.0018 | 0.0069 | 0.0099 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0047 | 0.0005 | 0.0005 | 0.0003 | 0.0049 | 0.0022 | 0.0036 | 0.0005 | 0.0009 | 0.0043 | 0.0089 | 0.0019 | 0.0187 | 0.0002 | 0.0034 | 0.0002 | 0.0011 | 0.0054 |
Endosome | 0.0105 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0.0037 | 0.0149 | 0.0008 | 0.0008 | 0.0235 | 0.0311 | 0.002 | 0.0306 | 0.0001 | 0.0107 | 0.0006 | 0.0021 | 0.0088 |
Golgi | 0.0031 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0094 | 0.0001 | 0.0002 | 0.0116 | 0.0175 | 0.0014 | 0.017 | 0 | 0.0022 | 0.0048 | 0.0022 | 0.0009 |
Lipid Particles | 0.0057 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0078 | 0 | 0.0002 | 0.0162 | 0.0061 | 0.0001 | 0.0181 | 0 | 0.0082 | 0.0006 | 0.0196 | 0.0014 |
Mitochondria | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.015 | 0.0001 | 0.0002 | 0.0234 | 0.0083 | 0.0055 | 0.0089 | 0.0001 | 0.0225 | 0.0021 | 0.0017 | 0.005 |
None | 0.0125 | 0.0005 | 0.0003 | 0.0004 | 0.0006 | 0.0013 | 0.0032 | 0.0006 | 0.0011 | 0.001 | 0.0011 | 0.0028 | 0.007 | 0.0006 | 0.001 | 0.0003 | 0.0012 | 0.0014 |
Nuclear Periphery | 0.0136 | 0.0016 | 0.0022 | 0.0018 | 0.0067 | 0.0087 | 0.0048 | 0.0008 | 0.0011 | 0.0042 | 0.0159 | 0.0036 | 0.0255 | 0.0008 | 0.0017 | 0.001 | 0.0027 | 0.008 |
Nucleolus | 0.0005 | 0 | 0 | 0 | 0 | 0.0007 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0007 | 0 | 0.0002 | 0 | 0 | 0.0001 |
Nucleus | 0.0219 | 0.0094 | 0.0184 | 0.0214 | 0.0177 | 0.054 | 0.0218 | 0.0077 | 0.0095 | 0.0136 | 0.0226 | 0.0184 | 0.0229 | 0.007 | 0.0139 | 0.0108 | 0.0188 | 0.018 |
Peroxisomes | 0.007 | 0 | 0 | 0 | 0 | 0.0001 | 0.007 | 0 | 0.0002 | 0.0015 | 0.0275 | 0.0002 | 0.0195 | 0 | 0.0022 | 0.0042 | 0.0347 | 0.0005 |
Punctate Nuclear | 0.0062 | 0.0002 | 0.0002 | 0.0003 | 0.0011 | 0.0107 | 0.0044 | 0.0003 | 0.0003 | 0.0027 | 0.0015 | 0.0147 | 0.0049 | 0.0002 | 0.0333 | 0.0002 | 0.0009 | 0.0307 |
Vacuole | 0.001 | 0.0004 | 0.0003 | 0.0002 | 0.0011 | 0.0009 | 0.0015 | 0.0003 | 0.0009 | 0.0022 | 0.0022 | 0.0007 | 0.0033 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0015 |
Vacuole Periphery | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0007 | 0.0008 | 0.0002 | 0.0032 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.9014 | 18.7287 | 22.133 | 27.885 | 32.6968 | 28.6943 | 23.1446 | 24.1417 | 21.8677 | 37.2519 |
Translational Efficiency | 0.6838 | 0.7434 | 0.5416 | 0.6558 | 0.7238 | 1.0973 | 0.7417 | 0.5972 | 0.9088 | 0.7722 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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