Standard name
Human Ortholog
Description Vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.9 0.87 0.96 0.91 0.93 0.95 0.9 0.93 0.91 0.89 0.94 0.98 0.99 0.9 0.88 0.92 0.2 0.45 0.33 0.25 0.26 0.23
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.14 0 0.05 0.09 0.12 0.13 0.12 0.14 0.17 0 0 0 0 0 0 0.05 0 0 0 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.08 0 0.08 0.14 0 0 0 0 0.05 0 0.08 0 0 0.12 0.14 0.13 0.6 0.45 0.57 0.69 0.64 0.65
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud 0 0 0 1 3 7 12 12 9 6 15 0 0 0 0 1 0 0 0 0 0 4 2
Bud Neck 0 0 0 0 0 2 1 3 1 3 1 5 0 2 1 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0
Cell Periphery 0 2 0 1 1 1 0 1 0 0 2 0 0 2 1 1 0 0 0 0 0 0 0
Cytoplasm 87 131 116 332 517 578 757 691 579 501 699 246 415 604 238 173 166 27 66 46 24 66 72
Endoplasmic Reticulum 3 2 1 0 0 1 2 0 1 0 0 0 1 0 10 8 11 2 1 2 0 2 2
Endosome 0 1 0 0 0 3 3 3 3 0 3 0 1 0 1 1 0 1 2 1 0 1 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 2 0 0 0 0 2
Mitochondria 9 1 19 7 31 55 97 98 72 74 134 0 0 0 9 3 1 7 4 3 3 7 18
Nucleus 4 0 0 1 2 6 0 14 6 3 7 1 0 2 0 1 1 2 0 0 1 0 1
Nuclear Periphery 0 0 1 2 0 1 8 6 5 0 1 0 0 0 0 1 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 1 0 1 2 6 2 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 1 2 3 3 4 7 0 0 0 0 0 0 2 0 0 0 1 1
Vac/Vac Membrane 14 12 5 29 80 19 15 13 17 30 31 20 9 7 31 28 23 81 65 80 68 162 205
Unique Cell Count 111 145 134 346 567 619 795 766 621 548 786 263 422 609 264 197 181 134 148 142 99 255 316
Labelled Cell Count 119 149 142 373 636 675 899 845 698 629 903 272 426 617 293 217 203 134 148 142 99 255 316


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.7 3.6 4.1 3.9 3.6 3.2 3.4 3.1 3.3 3.0 5.0 5.0 4.9 5.3 5.4 5.7 5.3 5.7 5.5
Std Deviation (1e-4) 0.8 1.4 1.2 4.7 1.5 1.4 1.5 1.5 1.2 1.5 0.9 0.8 1.4 1.6 1.4 1.0 1.5 0.8 2.5 1.6
Intensity Change (Log2) 0.21 0.12 0.01 -0.17 -0.06 -0.2 -0.1 -0.26 0.49 0.49 0.46 0.56 0.59 0.66 0.56 0.67 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 2.6 3.1 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2252 0.4011 0.1801 -0.2521 1.0011 0.421 0.8672 1.7489 1.6086 1.4848 1.0895 1.6107 -0.7168 0.3335 -0.2858 0.1431 -0.5865 -0.0072
Actin 0.0247 0.0001 0.0017 0.02 0.0013 0.0026 0.0166 0 0.0142 0.0001 0.0009 0.0001 0.0027 0.0001 0.0001 0 0 0
Bud 0.0005 0.0074 0.0011 0.0007 0.0004 0.0062 0.0008 0.0013 0.001 0.0007 0.0004 0.0005 0.0003 0.0007 0.003 0.0005 0.0003 0.0003
Bud Neck 0.0033 0.0021 0.0033 0.0005 0.0011 0.0034 0.0018 0.0012 0.0015 0.0014 0.0009 0.0017 0.0025 0.0007 0.0011 0.0003 0.0006 0.0012
Bud Periphery 0.0008 0.0026 0.0021 0.001 0.0007 0.0024 0.0037 0.0038 0.0026 0.0016 0.0007 0.0015 0.0005 0.0016 0.0031 0.0008 0.0003 0.001
Bud Site 0.0109 0.0244 0.0105 0.0021 0.0008 0.0017 0.0024 0.0017 0.0061 0.0016 0.0009 0.0007 0.0029 0.0017 0.004 0.0003 0.0013 0.0004
Cell Periphery 0.0013 0.001 0.0008 0.0002 0.0003 0.0008 0.0017 0.0019 0.0023 0.0009 0.0014 0.0017 0.0012 0.001 0.0008 0.0005 0.0005 0.0011
Cytoplasm 0.106 0.1556 0.1194 0.1334 0.0705 0.1232 0.0522 0.0327 0.0346 0.014 0.0408 0.0336 0.0677 0.0827 0.1084 0.0592 0.0852 0.0313
Cytoplasmic Foci 0.0673 0.0458 0.0591 0.0554 0.0753 0.0882 0.0323 0.0212 0.0267 0.0277 0.0273 0.0146 0.0224 0.0161 0.0212 0.0307 0.0153 0.0136
Eisosomes 0.0007 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0006 0 0 0 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.0082 0.0069 0.004 0.0013 0.0065 0.0034 0.0064 0.0082 0.0053 0.0021 0.0018 0.0033 0.0083 0.0113 0.0051 0.0021 0.002 0.0039
Endosome 0.218 0.1778 0.228 0.2746 0.2568 0.2133 0.2311 0.1544 0.2226 0.3027 0.2487 0.1793 0.2296 0.1545 0.1988 0.2492 0.147 0.1746
Golgi 0.0109 0.0031 0.0078 0.0141 0.0159 0.0064 0.0154 0.0033 0.0092 0.0085 0.0073 0.0046 0.0037 0.0019 0.0034 0.0016 0.0015 0.002
Lipid Particles 0.0266 0.0094 0.0241 0.043 0.0748 0.0319 0.0141 0.0108 0.0175 0.0129 0.0039 0.009 0.0193 0.0056 0.0054 0.0018 0.0022 0.0049
Mitochondria 0.015 0.0093 0.008 0.0245 0.0142 0.0052 0.0217 0.0098 0.0357 0.0335 0.0296 0.0056 0.0045 0.003 0.0199 0.0017 0.0006 0.0015
None 0.2652 0.2463 0.2292 0.1056 0.0709 0.125 0.1088 0.0683 0.0741 0.022 0.0666 0.0302 0.1526 0.0924 0.0846 0.0619 0.1211 0.0351
Nuclear Periphery 0.0077 0.0057 0.0064 0.002 0.039 0.0186 0.0115 0.0162 0.0063 0.0031 0.0025 0.009 0.0132 0.0228 0.0058 0.0036 0.0039 0.006
Nucleolus 0.0026 0.002 0.0033 0.0008 0.003 0.0038 0.0036 0.0027 0.0041 0.0057 0.0019 0.0024 0.0028 0.0018 0.0015 0.0009 0.001 0.0013
Nucleus 0.005 0.0076 0.0079 0.002 0.0257 0.0102 0.0119 0.0077 0.0082 0.0061 0.0041 0.0107 0.0052 0.0073 0.0047 0.0032 0.017 0.0049
Peroxisomes 0.0139 0.0007 0.0105 0.0332 0.0417 0.0072 0.0024 0.003 0.0041 0.0028 0.0007 0.0022 0.0017 0.0003 0.0008 0.0004 0.0003 0.0009
Punctate Nuclear 0.0061 0.0012 0.002 0.001 0.0049 0.005 0.0035 0.0005 0.0008 0.0018 0.0006 0.0003 0.0027 0.0005 0.0005 0.0008 0.0003 0.0004
Vacuole 0.1837 0.2702 0.2373 0.2385 0.2625 0.3133 0.4065 0.5663 0.4376 0.4814 0.5084 0.6094 0.3957 0.5287 0.4606 0.4739 0.5453 0.6239
Vacuole Periphery 0.0213 0.0206 0.0334 0.046 0.0337 0.0282 0.0512 0.0848 0.0848 0.0695 0.0505 0.0795 0.0601 0.0652 0.0671 0.1064 0.0543 0.0918

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.2193 21.3766 31.7247 29.9746 38.3859 26.1757 19.0408 27.7864 30.8216 24.8211
Translational Efficiency 2.6004 1.6436 1.2294 1.9689 1.568 1.7296 1.9164 1.5894 1.6749 1.8858

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1494 1678 1114 978 1453 1426 1495 1770 2947 3104 2609 2748

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 632.09 768.51 825.14 935.69 620.29 703.18 862.79 976.28 626.27 738.50 846.71 961.83
Standard Deviation 67.57 110.03 108.17 133.21 83.23 95.01 106.09 134.12 75.93 108.41 108.59 135.20
Intensity Change Log 2 0.281934 0.384509 0.565901 0.180951 0.476067 0.654352 0.232801 0.430583 0.610388

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000501 0.000276 0.000231 0.000826 0.000096 0.000570 0.000280 0.000301 0.000301 0.000411 0.000259 0.000488
Bud Neck 0.022826 0.023310 0.002994 0.011357 0.016769 0.045972 0.008362 0.011993 0.019840 0.033721 0.006070 0.011767
Bud Site 0.021815 0.015811 0.011154 0.026015 0.004604 0.018506 0.010353 0.029300 0.013329 0.017049 0.010695 0.028131
Cell Periphery 0.000941 0.000116 0.000136 0.000098 0.000119 0.000168 0.000126 0.000077 0.000536 0.000140 0.000131 0.000085
Cytoplasm 0.440784* 0.361611* 0.341368* 0.142994* 0.474563* 0.309067* 0.509642* 0.103483* 0.457438* 0.337472* 0.437792* 0.117545*
Cytoplasmic Foci 0.254301* 0.168627 0.010741 0.067234 0.230590* 0.276444* 0.032859 0.064259 0.242611* 0.218159* 0.023415 0.065318
Eisosomes 0.000397 0.000091 0.000033 0.000028 0.000164 0.000249 0.000044 0.000017 0.000282 0.000163 0.000039 0.000021
Endoplasmic Reticulum 0.001057 0.001734 0.003418 0.003749 0.000698 0.000941 0.003965 0.001955 0.000880 0.001369 0.003731 0.002594
Endosome 0.016244 0.048390 0.002642 0.086555 0.020549 0.063799 0.009023 0.135033 0.018366 0.055469 0.006299 0.117780
Golgi 0.003602 0.003608 0.000096 0.005165 0.001752 0.009210 0.000696 0.011013 0.002690 0.006181 0.000440 0.008932
Lipid Particles 0.017274 0.004584 0.001457 0.003969 0.009402 0.010746 0.002928 0.003137 0.013393 0.007415 0.002300 0.003433
Mitochondria 0.006003 0.003487 0.000542 0.000998 0.006346 0.015156 0.000853 0.004102 0.006172 0.008848 0.000720 0.002997
Mitotic Spindle 0.000574 0.002257 0.008055 0.010425 0.000739 0.008329 0.006155 0.011206 0.000655 0.005046 0.006966 0.010928
None 0.004958 0.001658 0.008112 0.000294 0.003617 0.001091 0.002305 0.000246 0.004297 0.001397 0.004784 0.000263
Nuclear Periphery 0.000487 0.000476 0.002015 0.001838 0.000564 0.000552 0.002765 0.001043 0.000525 0.000511 0.002445 0.001326
Nuclear Periphery Foci 0.000254 0.000561 0.001378 0.004941 0.000363 0.000526 0.004620 0.001680 0.000307 0.000545 0.003236 0.002840
Nucleolus 0.001275 0.001263 0.000749 0.001201 0.001895 0.001129 0.000741 0.001434 0.001581 0.001202 0.000745 0.001351
Nucleus 0.131769* 0.062056 0.551321* 0.017181 0.141320* 0.049566 0.226047* 0.007879 0.136478* 0.056318 0.364933* 0.011190
Peroxisomes 0.006511 0.005539 0.000209 0.003217 0.005667 0.024123 0.001138 0.002751 0.006095 0.014076 0.000741 0.002917
Vacuole 0.067305 0.288934* 0.053054 0.592829* 0.078436 0.159856 0.176619 0.575694* 0.072793 0.229635* 0.123859 0.581793*
Vacuole Periphery 0.001123 0.005614 0.000294 0.019088 0.001747 0.004001 0.000480 0.033395 0.001431 0.004873 0.000401 0.028303

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.74 0.62 -0.58 -1.63 -1.51 -5.05 -3.40 -3.99 2.38 -0.43 -0.67 0.04 -0.88 -0.48 -1.53
Bud Neck -0.18 12.99 6.41 6.60 -8.42 -10.17 5.56 2.97 12.24 -3.41 -7.97 12.44 6.79 13.61 -7.35
Bud Site 1.90 3.76 -0.59 -3.26 -5.33 -8.26 -2.64 -11.58 -3.96 -8.57 -1.97 2.55 -6.30 -5.50 -9.98
Cell Periphery 1.60 1.56 1.65 1.80 3.85 -4.08 -0.18 6.12 8.53 3.69 1.52 1.56 1.75 7.20 5.41
Cytoplasm 8.25 13.57 29.06 20.03 13.11 16.35 1.40 41.10 22.43 39.87* 17.14 8.83 51.50 31.83 37.95
Cytoplasmic Foci 12.38 44.88 31.55 20.16 -15.96 -5.89 37.29 29.87 34.78 -11.26 4.79 57.35 43.63 38.03 -18.96
Eisosomes 11.41 13.68 14.29 7.59 5.54 -4.35 8.82 11.27 16.19 14.44 7.18 15.98 17.73 17.70 14.89
Endoplasmic Reticulum -3.30 -14.50 -6.04 -4.56 3.59 -1.44 -15.79 -5.42 -3.64 8.71 -3.69 -20.79 -7.58 -5.17 9.33
Endosome -14.10 15.07 -13.79 -4.81 -17.78 -14.73 8.66 -25.62 -12.79 -30.29* -20.13 14.46 -29.28 -14.76 -34.76*
Golgi 0.17 8.66 -0.11 -0.22 -4.31 -9.90 3.51 -7.42 -0.28 -8.38 -7.17 8.25 -6.20 -1.59 -9.49
Lipid Particles 11.16 13.94 11.66 1.53 -7.40 -1.18 8.79 8.05 7.81 -0.93 7.41 16.21 14.19 7.91 -4.50
Mitochondria 2.12 5.15 4.82 4.05 -2.05 -4.51 4.63 1.88 6.63 -3.79 -2.34 6.85 3.76 6.58 -4.03
Mitotic Spindle -2.28 -4.46 -5.18 -4.24 -0.92 -4.56 -4.08 -6.60 -1.69 -2.97 -5.09 -6.06 -8.36 -4.41 -2.84
None 3.25 -0.93 4.95 2.88 6.38 4.61 2.43 6.46 4.13 7.57 4.91 0.54 7.39 4.15 8.56
Nuclear Periphery -0.04 -19.39 -3.61 -3.60 1.54 0.08 -13.68 -3.53 -2.25 8.69 0.09 -20.29 -5.09 -4.53 7.29
Nuclear Periphery Foci -3.72 -10.89 -8.63 -8.04 -5.67 -0.91 -7.57 -5.21 -3.96 4.84 -2.68 -9.52 -10.00 -8.74 1.24
Nucleolus 0.47 2.00 0.70 0.44 -3.95 2.72 3.78 1.21 -2.08 -3.77 2.06 3.92 1.09 -1.48 -4.89
Nucleus 15.63 -43.64 27.19 13.46 58.10 18.96 -14.99 31.01 15.58 42.18* 24.35 -37.49 42.45 22.19 61.87
Peroxisomes 1.46 12.00 5.34 4.08 -6.44 -10.48 7.15 4.85 13.02 -5.10 -8.70 12.81 7.63 13.57 -8.50
Vacuole -30.75* -3.44 -56.14 -27.40 -50.53* -13.46 -19.61 -66.05* -48.85 -40.27* -31.83 -18.35 -87.50* -50.31 -62.37*
Vacuole Periphery -9.64 8.24 -7.46 -4.78 -7.97 -6.40 5.28 -17.96 -16.49 -19.08* -11.38 7.92 -19.20 -15.99 -20.26