Standard name
Description DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity is stimulated by RPA and Sgs1p-Top3p-Rmi1p; involved in telomere stability and regulation of mitotic recombination

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.06 0.12 0.17 0.12 0.11 0.14 0.12 0.06 0.1 0.27 0.34 0.07 0.11 0.12 0.06 0 0 0.17 0.24 0.35
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0 0.07 0 0.14 0.17 0.41 0.31 0.72 0.4 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.64 0.7 0.72 0.63 0.68 0.68 0.57 0.65 0.56 0.62 0.61 0.58 0.75 0.71 0.74 0.63 0.63 0.49 0.37 0.29 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0
Vac/Vac Membrane 0.14 0.29 0.09 0.16 0.12 0.12 0.08 0.1 0.05 0.07 0.15 0.1 0.18 0.14 0.13 0.18 0.19 0.27 0.31 0.34 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 0 3 0 0 2 0 3 2 2 0 0 0 0 0 1 2 1 0 1 2
Bud Neck 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 2
Bud Site 0 0 0 0 0 0 5 2 2 2 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 11 16 54 37 38 35 41 8 19 30 43 17 34 32 5 6 2 2 25 58
Endoplasmic Reticulum 0 2 2 0 1 0 0 0 1 0 0 1 8 13 9 0 0 0 0 0 1
Endosome 0 3 1 9 5 10 2 4 0 2 6 2 4 6 3 1 8 1 0 1 2
Golgi 0 1 0 0 0 0 0 1 0 0 0 0 2 5 3 0 1 0 0 0 0
Mitochondria 27 3 9 0 41 57 107 108 104 78 0 0 0 5 7 2 2 0 0 2 6
Nucleus 53 135 99 196 206 227 148 223 81 121 67 73 171 217 195 62 130 33 5 31 23
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 1 3 0 0 0 1 0 1 0 0 0 0 4 3 2 2 8 1 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0
SpindlePole 1 4 5 11 5 9 5 8 1 3 1 2 3 3 1 4 7 4 0 3 7
Vac/Vac Membrane 12 56 12 50 35 39 20 33 7 13 17 13 40 44 35 17 39 18 4 36 56
Unique Cell Count 83 192 137 311 301 336 258 343 145 194 110 125 227 306 264 100 209 68 16 107 171
Labelled Cell Count 99 218 148 320 331 383 322 425 206 242 121 134 250 333 287 100 209 68 16 107 171


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.6 5.5 6.5 5.1 5.3 4.7 4.7 4.0 4.3 6.3 6.0 10.2 10.2 9.1 5.7 5.7
Std Deviation (1e-4) 0.6 0.8 1.1 2.0 0.9 1.3 1.3 1.4 1.1 0.9 1.4 1.0 1.6 1.8 1.6 1.3 1.3
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Top3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available