Standard name
Description Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.92 0.85 0.88 0.73 0.71 0.42 0.25 0.29 0.15 0.15 0.24 0.9 0.9 0.79 0.88 0.78 0.81 0.68 0.58 0.7 0.76 0.73 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.12 0.09 0.16 0.36 0.3 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.06 0.11 0.18 0.11 0.14 0.13 0.14 0 0 0 0 0 0 0.21 0.28 0.08 0.12 0.12 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.1 0.2 0.13 0.28 0.26 0.44 0.55 0.5 0.49 0.52 0.46 0.14 0.18 0.33 0.25 0.38 0.32 0.05 0.09 0.08 0.09 0.07 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 4 0 0 3 4 5 0 0 0 0 1 0 1 1 1 1 5 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 1 2 1 2 0 3 1 1 0 1 1 0 1 0 0 0 0 0 0 0
Cytoplasm 126 84 204 175 151 200 156 102 62 45 30 56 239 414 356 21 56 30 97 63 174 155 367 293
Endoplasmic Reticulum 1 0 3 3 2 4 2 2 0 1 2 2 3 1 2 1 5 2 2 0 7 0 5 2
Endosome 0 0 2 0 2 2 3 12 5 4 2 0 0 0 0 0 1 1 0 0 6 1 7 7
Golgi 1 1 7 15 15 4 3 7 1 2 0 0 6 11 25 1 3 2 0 0 0 0 0 0
Mitochondria 1 0 1 0 3 14 46 37 35 109 60 71 0 0 0 0 0 0 0 0 1 0 0 0
Nucleus 0 0 4 0 3 16 43 74 23 41 26 33 2 1 3 0 0 0 30 31 19 25 58 46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 6 0 9 9
Nucleolus 0 0 0 1 3 4 5 10 3 3 1 2 0 0 2 0 1 2 0 0 1 0 2 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 6 5
Vac/Vac Membrane 13 9 49 26 58 73 165 225 107 148 103 106 38 84 150 6 27 12 6 9 20 17 34 39
Unique Cell Count 136 91 240 200 207 282 374 410 216 303 199 232 267 461 448 24 72 37 144 109 250 206 501 416
Labelled Cell Count 142 94 270 221 240 319 429 475 236 360 230 276 288 512 539 29 95 49 144 109 250 206 501 416


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.0 32.5 22.8 22.7 18.2 14.5 12.5 12.3 10.8 9.7 9.2 9.3 28.9 28.0 25.9 30.8 31.8 34.2 28.7 30.0 31.0
Std Deviation (1e-4) 4.9 5.8 5.4 4.0 4.1 3.2 3.3 3.6 2.6 2.9 2.4 3.0 6.1 5.8 5.8 12.7 9.4 11.4 6.5 6.2 6.5
Intensity Change (Log2) – – – -0.01 -0.33 -0.65 -0.86 -0.89 -1.08 -1.23 -1.32 -1.29 0.34 0.3 0.19 0.43 0.48 0.59 0.34 0.4 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 -3.1 -3.8 -10.6 -14.8 -12.2 -16.3 -14.6 -13.3 1.5 1.9 -1.8 0.3 -1.4 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 1.8 0 0 0 0 0 0 0 0 0 0
Golgi 2.2 2.1 -1.2 0 -1.0 0 0 0 0 -0.5 -0.4 1.6 0 0 0
Mitochondria 0 0 3.1 5.4 4.5 6.2 10.2 9.0 9.1 0 0 0 0 0 0
Nucleus 0 0 2.4 4.5 6.2 4.1 5.0 4.7 5.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 1.9 1.5 6.0 8.6 6.5 6.8 6.9 5.8 -1.8 -0.7 3.6 0.5 3.0 1.6