Standard name
Description Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.11 0.14 0.25 0.25 0.18 0.26 0 0.09 0.14 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.09 0.07 0.08 0.09 0 0 0 0 0 0 0.06 0.05 0 0 0.07 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0.09 0.11 0.05 0.09 0 0 0.07 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.98 0.94 0.97 0.95 0.9 0.66 0.56 0.29 0.27 0.16 0.19 0.9 0.76 0.72 0.91 0.92 0.82 0.87 0.8 0.84 0.83 0.76 0.78
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.11 0 0.09 0.07 0.15 0 0.06 0.06 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0.06 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.16 0.14 0.4 0.35 0.55 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.08 0.11 0.12 0.11 0.14 0.17 0.18 0.06 0.06 0 0 0.08 0.13 0 0 0 0.05 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 8 9 66 109 153 158 110 153 1 24 34 0 0 0 1 3 2 0 3 1
Bud 0 0 2 4 6 14 26 56 45 47 54 4 9 9 1 0 0 8 11 15 5 16 5
Bud Neck 0 0 2 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0
Bud Site 0 0 0 1 0 2 1 7 2 3 1 1 0 0 0 0 0
Cell Periphery 1 0 4 5 7 23 41 52 68 29 56 0 10 17 4 5 2 0 1 1 0 0 0
Cytoplasm 129 210 465 412 452 396 439 179 168 97 116 211 201 175 90 94 37 117 190 411 143 175 164
Endoplasmic Reticulum 0 2 10 5 3 32 83 26 58 43 88 1 17 15 3 3 1 0 3 1 3 6 6
Endosome 0 1 0 0 3 12 12 16 13 6 15 3 4 2 2 0 0 0 4 3 1 0 0
Golgi 1 1 1 2 1 3 13 33 35 20 58 0 10 10 0 1 0 0 1 2 0 1 2
Mitochondria 0 2 0 8 15 99 111 242 219 338 141 2 5 5 4 2 2 0 2 14 4 4 6
Nucleus 0 1 4 3 1 8 12 3 8 7 6 0 1 0 1 1 1 0 5 4 1 2 2
Nuclear Periphery 0 0 0 0 1 1 4 2 5 8 5 1 1 0 0 0 0 0 0 2 0 0 0
Nucleolus 0 0 2 0 2 1 5 2 3 12 5 1 1 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 11 7 29 10 1 5 2 3 5 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 2 3 4 6 7 2 3 10 0 1 2 0 0 0 2 2 12 1 2 2
Vac/Vac Membrane 1 11 3 12 42 65 93 69 84 108 107 15 15 3 3 8 6 1 8 9 8 11 13
Unique Cell Count 131 224 478 432 503 602 780 611 622 618 596 234 263 242 99 102 45 135 240 489 173 231 212
Labelled Cell Count 132 228 494 464 546 739 962 876 878 832 820 242 302 278 109 114 49 135 240 489 173 231 212


Cytoplasm, Nucleus, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 9.0 6.5 5.8 7.2 6.5 7.5 8.0 8.4 7.8 9.1 6.9 10.5 11.5 10.2 9.6 9.4 5.8 6.5 7.1
Std Deviation (1e-4) 2.4 4.1 1.5 1.5 1.9 1.9 2.1 2.7 2.6 2.5 2.9 2.0 3.5 3.9 4.2 2.7 2.6 1.7 2.5 2.8
Intensity Change (Log2) -0.18 0.14 -0.01 0.2 0.29 0.36 0.25 0.47 0.07 0.68 0.82 0.65 0.56 0.53 -0.18 0.0 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 7.3 8.4 11.7 11.8 9.6 11.8 0 6.4 8.2 0 0 0
Bud 0 0 2.6 3.4 6.4 5.5 5.7 6.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.8 3.1 4.1 5.7 6.7 3.7 6.1 0 0 4.7 0 0 0
Cytoplasm -1.5 -4.7 -12.8 -15.7 -22.6 -23.4 -26.8 -25.4 -4.1 -9.0 -10.1 -3.0 -2.5 -4.9
Endoplasmic Reticulum -1.1 -2.0 2.7 5.6 2.0 4.9 3.7 7.2 0 3.0 2.8 0 0 0
Endosome 0 0 3.1 0 3.6 3.2 0 3.5 0 0 0 0 0 0
Golgi 0 0 0 2.4 4.9 5.0 3.6 6.8 0 0 0 0 0 0
Mitochondria 0 3.8 9.3 8.6 15.6 14.5 19.4 11.4 0 0 0 0 0 0
Nucleus 0 0 0.8 1.1 0 0.7 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2.2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 4.8 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.5 5.8 6.8 7.3 7.0 7.8 9.2 9.3 4.6 4.3 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gsy2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available