Standard name
Human Ortholog
Description Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.11 0.14 – 0.25 0.25 0.18 0.26 0 0.09 0.14 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0.09 0.07 0.08 0.09 0 0 0 0 0 0 0.06 0.05 0 0 0.07 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 – 0.09 0.11 0.05 0.09 0 0 0.07 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.98 0.94 0.97 0.95 0.9 0.66 0.56 – 0.29 0.27 0.16 0.19 0.9 0.76 0.72 0.91 0.92 0.82 0.87 0.8 0.84 0.83 0.76 0.78
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.11 – 0 0.09 0.07 0.15 0 0.06 0.06 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0.05 0.06 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.16 0.14 – 0.4 0.35 0.55 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.08 0.11 0.12 – 0.11 0.14 0.17 0.18 0.06 0.06 0 0 0.08 0.13 0 0 0 0.05 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 8 9 66 109 – 153 158 110 153 1 24 34 0 0 0 1 3 2 0 3 1
Bud 0 0 2 4 6 14 26 – 56 45 47 54 4 9 9 1 0 0 8 11 15 5 16 5
Bud Neck 0 0 2 2 1 2 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0
Bud Site 0 0 0 1 0 2 1 – 7 2 3 1 1 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 4 5 7 23 41 – 52 68 29 56 0 10 17 4 5 2 0 1 1 0 0 0
Cytoplasm 129 210 465 412 452 396 439 – 179 168 97 116 211 201 175 90 94 37 117 190 411 143 175 164
Endoplasmic Reticulum 0 2 10 5 3 32 83 – 26 58 43 88 1 17 15 3 3 1 0 3 1 3 6 6
Endosome 0 1 0 0 3 12 12 – 16 13 6 15 3 4 2 2 0 0 0 4 3 1 0 0
Golgi 1 1 1 2 1 3 13 – 33 35 20 58 0 10 10 0 1 0 0 1 2 0 1 2
Mitochondria 0 2 0 8 15 99 111 – 242 219 338 141 2 5 5 4 2 2 0 2 14 4 4 6
Nucleus 0 1 4 3 1 8 12 – 3 8 7 6 0 1 0 1 1 1 0 5 4 1 2 2
Nuclear Periphery 0 0 0 0 1 1 4 – 2 5 8 5 1 1 0 0 0 0 0 0 2 0 0 0
Nucleolus 0 0 2 0 2 1 5 – 2 3 12 5 1 1 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 11 7 – 29 10 1 5 2 3 5 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 2 3 4 6 – 7 2 3 10 0 1 2 0 0 0 2 2 12 1 2 2
Vac/Vac Membrane 1 11 3 12 42 65 93 – 69 84 108 107 15 15 3 3 8 6 1 8 9 8 11 13
Unique Cell Count 131 224 478 432 503 602 780 611 622 618 596 234 263 242 99 102 45 135 240 489 173 231 212
Labelled Cell Count 132 228 494 464 546 739 962 876 878 832 820 242 302 278 109 114 49 135 240 489 173 231 212


Cytoplasm, Nucleus, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 9.0 6.5 5.8 7.2 6.5 7.5 – 8.0 8.4 7.8 9.1 6.9 10.5 11.5 10.2 9.6 9.4 5.8 6.5 7.1
Std Deviation (1e-4) 2.4 4.1 1.5 1.5 1.9 1.9 2.1 – 2.7 2.6 2.5 2.9 2.0 3.5 3.9 4.2 2.7 2.6 1.7 2.5 2.8
Intensity Change (Log2) – – – -0.18 0.14 -0.01 0.2 – 0.29 0.36 0.25 0.47 0.07 0.68 0.82 0.65 0.56 0.53 -0.18 0.0 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 7.3 8.4 – 11.7 11.8 9.6 11.8 0 6.4 8.2 0 0 0
Bud 0 0 2.6 3.4 – 6.4 5.5 5.7 6.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.8 3.1 4.1 – 5.7 6.7 3.7 6.1 0 0 4.7 0 0 0
Cytoplasm -1.5 -4.7 -12.8 -15.7 – -22.6 -23.4 -26.8 -25.4 -4.1 -9.0 -10.1 -3.0 -2.5 -4.9
Endoplasmic Reticulum -1.1 -2.0 2.7 5.6 – 2.0 4.9 3.7 7.2 0 3.0 2.8 0 0 0
Endosome 0 0 3.1 0 – 3.6 3.2 0 3.5 0 0 0 0 0 0
Golgi 0 0 0 2.4 – 4.9 5.0 3.6 6.8 0 0 0 0 0 0
Mitochondria 0 3.8 9.3 8.6 – 15.6 14.5 19.4 11.4 0 0 0 0 0 0
Nucleus 0 0 0.8 1.1 – 0 0.7 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 2.2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 4.8 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.5 5.8 6.8 7.3 – 7.0 7.8 9.2 9.3 4.6 4.3 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4592 6.5276 5.1009 4.6468 4.1725 6.0527 6.6363 6.4148 5.3232 4.2167 4.3574 5.5207 8.8368 9.6254 8.2633 7.4699 7.6395 7.9929
Actin 0.0187 0.0104 0.013 0.0016 0.0076 0.0072 0.0059 0.0194 0.013 0.0088 0.0682 0.0067 0.0113 0.0328 0.0156 0.0139 0.0002 0.0116
Bud 0.0016 0.0123 0.0049 0.0137 0.0066 0.0016 0.0003 0.0084 0.0034 0.0039 0.0076 0.0019 0.0026 0.0042 0.0016 0.0053 0.0001 0.0004
Bud Neck 0.007 0.0007 0.0013 0.0009 0.002 0.0043 0.0005 0.0005 0.0019 0.0013 0.0016 0.0035 0.0032 0.001 0.0038 0.0007 0.0006 0.0023
Bud Periphery 0.0016 0.0072 0.0029 0.0051 0.0091 0.0017 0.0002 0.004 0.0026 0.0039 0.0061 0.0018 0.0021 0.0031 0.0008 0.0033 0 0.0003
Bud Site 0.0065 0.0094 0.0052 0.0007 0.0023 0.001 0.0012 0.0122 0.0133 0.0006 0.0027 0.0003 0.0036 0.0136 0.0068 0.0007 0 0.0005
Cell Periphery 0.0009 0.0006 0.0006 0.0001 0.0007 0.0002 0.0002 0.0003 0.0007 0.0002 0.0003 0.0001 0.0005 0.0136 0.0002 0.0001 0 0.0061
Cytoplasm 0.78 0.884 0.8544 0.8797 0.8548 0.8951 0.8607 0.8849 0.8693 0.8615 0.8226 0.91 0.8526 0.8734 0.8941 0.9034 0.9792 0.928
Cytoplasmic Foci 0.0193 0.0063 0.0102 0.0035 0.007 0.0077 0.0271 0.0123 0.0174 0.0111 0.0164 0.0141 0.0189 0.0054 0.0286 0.0151 0.0028 0.0126
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0.0051 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0066 0.003 0.0064 0.003 0.0085 0.0041 0.0028 0.0017 0.0033 0.0075 0.0036 0.0027 0.0044 0.0029 0.0034 0.0077 0.0009 0.0031
Endosome 0.0183 0.0036 0.0144 0.0024 0.0206 0.007 0.0065 0.0034 0.0054 0.011 0.0202 0.0055 0.0194 0.002 0.0085 0.0107 0.0008 0.0024
Golgi 0.004 0.0003 0.0046 0.0001 0.0041 0.0006 0.0015 0.0014 0.0007 0.0008 0.0049 0.0003 0.0034 0.0013 0.0039 0.0017 0 0.0004
Lipid Particles 0.0079 0.0002 0.0032 0 0.0037 0.0004 0.0041 0.0001 0.0012 0.0003 0.0021 0.0001 0.012 0.0002 0.0029 0.0007 0 0.0007
Mitochondria 0.0073 0.0005 0.0081 0.0005 0.0079 0.0006 0.0081 0.0013 0.0005 0.0012 0.0031 0.0005 0.0021 0.0005 0.0026 0.0006 0.0001 0.0051
None 0.0253 0.0401 0.0268 0.0236 0.0352 0.0225 0.0323 0.0195 0.0329 0.0672 0.008 0.0165 0.007 0.0223 0.0064 0.0242 0.0072 0.0032
Nuclear Periphery 0.0205 0.0031 0.0055 0.0066 0.005 0.0056 0.004 0.0021 0.0031 0.0034 0.007 0.0075 0.0096 0.0019 0.0021 0.003 0.0009 0.0031
Nucleolus 0.0009 0.0001 0.0027 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0005 0.0001 0.0003 0.0002 0.0003 0 0 0
Nucleus 0.0536 0.0139 0.0194 0.0501 0.0159 0.0339 0.0361 0.0179 0.0233 0.009 0.0104 0.0204 0.0328 0.0134 0.0109 0.0051 0.0064 0.0168
Peroxisomes 0.005 0.0002 0.0069 0.0001 0.0005 0.0002 0.0031 0.0011 0.0025 0.0012 0.002 0.0004 0.005 0.0002 0.0025 0.0004 0 0.0002
Punctate Nuclear 0.0096 0.001 0.0046 0.0054 0.0013 0.0027 0.0035 0.0081 0.0032 0.0027 0.0096 0.0038 0.0056 0.0011 0.0026 0.0014 0.0004 0.0021
Vacuole 0.0035 0.0025 0.004 0.0024 0.0055 0.0027 0.0013 0.001 0.0018 0.0033 0.0024 0.0025 0.0028 0.0018 0.0017 0.0017 0.0003 0.0005
Vacuole Periphery 0.0018 0.0003 0.0008 0.0004 0.0013 0.0006 0.0003 0.0002 0.0003 0.0008 0.0007 0.0014 0.0007 0.0002 0.0005 0.0004 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.6798 9.7799 9.6762 38.0284 31.0709 26.0338 11.8897 16.5262 43.7964 43.983
Translational Efficiency 0.6398 0.2236 0.497 1.0281 0.9278 0.5537 0.6296 0.6009 0.7643 0.6828

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress
Localization
Cell Percentages cytoplasm (65%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gsy2

Gsy2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gsy2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available