Standard name
Description Protein component of the synaptonemal complex axial elements; involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; promotes interhomolog recombination by phosphorylating Hop1p; also interacts with Mec3p and Ddc1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.99 0.98 0.96 0.96 0.96 0.89 0.79 0.81 0.98 0.99 0.99 0.57 0.59 0.57 0.89 0.91 0.77 0.85 0.76 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.11 0.06 0.28 0.33 0.21 0.08 0 0 0 0 0 0 0 0.07 0.06 0.09 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.21 0.18 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.28 0.28 0.3 0 0 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 1 1 3 0 3 3
Bud 0 1 0 0 1 2 2 2 4 2 4 0 0 0 0 0 0 1 1 7 0 6 7
Bud Neck 1 1 10 7 14 6 5 10 8 1 8 0 0 0 0 2 0 0 0 1 0 3 2
Bud Site 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0
Cell Periphery 3 2 1 2 2 0 4 3 1 0 0 0 1 2 1 1 0 1 1 5 0 3 3
Cytoplasm 189 302 492 332 503 328 455 362 258 142 208 114 331 321 232 198 169 174 280 338 57 252 154
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 2 0 20 12 17 0 0 3 0 5 4
Endosome 0 0 0 0 0 1 0 0 0 0 1 0 0 0 19 5 11 0 3 4 0 2 2
Golgi 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 3 0 0 0
Mitochondria 2 9 2 4 6 34 52 21 80 59 55 9 0 0 6 8 8 5 8 29 3 29 20
Nucleus 1 0 5 5 2 1 4 5 3 3 7 0 0 0 81 71 52 0 0 1 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 1 0 1 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 3 3 5 5 0 2 6 0 0 0 2 1 2 0 4 7 0 5 0
Vac/Vac Membrane 1 0 1 1 1 1 1 0 3 1 3 0 0 1 115 96 88 4 3 4 1 12 17
Unique Cell Count 193 307 498 336 511 340 474 376 289 180 258 116 333 323 410 338 297 196 308 438 68 333 222
Labelled Cell Count 197 316 515 351 532 376 529 410 358 211 296 123 335 325 478 396 348 196 308 438 68 333 222


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.0 4.8 4.3 4.9 3.8 4.2 4.5 3.4 3.1 3.6 4.1 4.2 4.3 6.4 6.6 6.1 4.0 3.9 4.4
Std Deviation (1e-4) 0.5 0.6 1.3 1.5 1.7 1.5 1.5 1.8 1.2 0.7 0.9 1.6 1.2 1.6 1.2 1.3 1.1 1.7 1.3 1.8
Intensity Change (Log2) -0.13 0.03 -0.32 -0.2 -0.09 -0.51 -0.64 -0.41 -0.21 -0.18 -0.14 0.42 0.46 0.35 -0.24 -0.29 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 12.5 12.5 12.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Red1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available