Standard name
Human Ortholog
Description Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0.06 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0.06 0.05 0.08 0.06 0.06 0 0.09 0.1 0.14 0.1 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.11 0.09 0.05 0.1 0.12 0.13 0.19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.23 0.68 0.61 0.48 0.68 0.67 0.67 0.75 0.72 0.76 0.78 0.08 0.07 0.22 0.06 0 0.12 0 0 0 0 0 0.05
Nucleus 0.63 0.68 0.6 0.6 0.41 0.38 0.35 0.44 0.32 0.42 0.44 0.37 0.77 0.73 0.6 0.62 0.49 0.38 0.39 0.64 0.43 0.39 0.29 0.15
Nuclear Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0
Nucleolus 0.31 0.16 0.18 0.18 0.23 0.2 0.21 0.17 0.19 0.2 0.16 0.17 0.11 0.15 0.18 0.21 0.29 0.45 0.25 0.13 0.17 0.25 0.19 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0.08 0.09 0.16 0.14 0.13 0.11 0 0.06 0 0.08 0 0 0 0.05 0.1 0.12 0.12 0.05 0.13 0.13 0.11 0.18
Vac/Vac Membrane 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0.06 0.07 0.07 0.16 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 2
Bud 2 0 3 7 5 8 16 15 14 18 7 13 0 1 2 0 0 0 4 0 2 0 4 2
Bud Neck 0 0 0 1 2 4 3 4 0 0 1 1 0 0 0 0 0 0 1 2 1 0 1 2
Bud Site 3 1 5 7 14 16 24 27 17 23 13 16 0 0 0 0 1 0
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 8 3 5 9 9 23 28 29 30 32 13 17 17 23 34 16 12 11 2 1 0 0 1 4
Endoplasmic Reticulum 0 0 0 2 1 0 0 0 0 0 0 1 0 0 0 1 2 2 1 0 0 0 0 1
Endosome 21 1 1 0 9 4 1 3 3 1 0 1 2 3 2 8 9 15 46 6 20 14 28 47
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 2 2 0 0 0 0 2
Mitochondria 19 23 133 161 151 269 334 363 282 368 173 308 15 15 54 9 6 16 6 1 4 0 6 13
Nucleus 293 67 117 159 128 153 175 238 122 213 99 146 142 166 150 95 73 52 207 78 90 47 61 38
Nuclear Periphery 19 5 2 1 4 3 7 4 6 10 6 9 3 3 4 4 2 2 25 1 10 0 1 7
Nucleolus 143 16 36 48 73 78 104 91 73 104 37 68 20 35 46 33 43 62 133 15 35 29 39 31
Peroxisomes 2 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 33 0 15 25 49 56 64 61 14 28 8 30 0 1 5 7 15 16 66 6 26 15 23 44
Vac/Vac Membrane 9 9 1 1 5 5 4 2 6 2 4 3 6 4 8 9 9 5 19 6 14 8 34 43
Unique Cell Count 465 99 196 264 312 398 499 538 377 509 227 394 184 227 249 154 148 138 535 123 211 121 211 254
Labelled Cell Count 553 125 318 421 450 619 760 838 567 801 362 613 205 251 305 185 174 184 535 123 211 121 211 254


Cytoplasm, Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 4.8 3.8 3.8 4.0 3.5 3.4 3.6 3.1 3.2 3.1 2.9 5.8 5.7 4.8 8.3 8.8 8.9 5.4 5.2 6.1
Std Deviation (1e-4) 0.9 1.5 0.7 1.0 1.1 0.8 0.9 1.1 1.1 1.2 1.0 1.0 1.7 1.3 1.3 2.0 2.2 2.1 1.4 1.1 2.1
Intensity Change (Log2) -0.0 0.07 -0.12 -0.16 -0.1 -0.27 -0.23 -0.28 -0.39 0.61 0.59 0.35 1.12 1.21 1.23 0.51 0.47 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8407 0.9471 1.1426 1.0642 0.6149 1.0407 2.0196 2.3106 2.5171 2.0349 2.6575 2.4438 1.2606 1.3137 1.2597 1.3541 1.1142 1.3024
Actin 0.0002 0.0005 0.0003 0.0007 0.0025 0.0004 0.0055 0.0003 0.0075 0.0001 0.0065 0.0068 0.0175 0.0003 0.0003 0.0005 0.0001 0.0005
Bud 0 0.0002 0.0001 0.0003 0.0003 0.0002 0.0003 0.0002 0.0014 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0011 0.0022 0.0025 0.0106 0.2169 0.0074 0.0012 0.0023 0.0022 0.0032 0.0025 0.0061 0.0009 0.0016 0.0019 0.0024 0.0249 0.0044
Bud Periphery 0.0001 0.0003 0.0003 0.0013 0.0008 0.0005 0.0005 0.0004 0.0024 0.0002 0.001 0.0008 0.0003 0.0003 0.0002 0.0003 0.0002 0.0005
Bud Site 0.0003 0.0032 0.0015 0.0018 0.0037 0.0007 0.0023 0.0014 0.0132 0.0007 0.001 0.0008 0.0007 0.0018 0.0009 0.001 0.0009 0.0003
Cell Periphery 0.0001 0.0002 0.0002 0.0005 0.0005 0.0001 0.0001 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0.0003 0.0003
Cytoplasm 0.0075 0.009 0.0046 0.0004 0.0006 0.0018 0.007 0.0043 0.0019 0.0001 0.0128 0.0014 0.0026 0.0125 0.0013 0.0005 0.0002 0.0028
Cytoplasmic Foci 0.0005 0.0005 0.002 0.0026 0.0105 0.0009 0.0169 0.0022 0.0197 0.0062 0.0176 0.0023 0.0052 0.0032 0.0017 0.0125 0.0037 0.003
Eisosomes 0 0 0 0.0001 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0007 0.002 0.0012 0.0009 0.0002 0.0003 0.0018 0.0006 0.003 0 0.0028 0.0056 0.0007 0.0016 0.0003 0.0004 0 0.0002
Endosome 0.0021 0.0023 0.0034 0.0017 0.0051 0.0011 0.017 0.0024 0.0117 0.0008 0.0279 0.0128 0.0077 0.0067 0.002 0.0078 0.0012 0.0017
Golgi 0.0001 0.0002 0.0005 0.0007 0.0037 0.0002 0.0042 0.0006 0.006 0.0008 0.0167 0.0062 0.0039 0.0007 0.0005 0.0046 0.0009 0.0004
Lipid Particles 0.001 0.0007 0.0072 0.005 0.0351 0.0013 0.026 0.0066 0.0278 0.013 0.0277 0.0123 0.0288 0.0106 0.0104 0.0651 0.0134 0.0059
Mitochondria 0.0004 0.0006 0.0005 0.0002 0.0012 0.0006 0.0116 0.0014 0.0133 0.0001 0.0085 0.0032 0.0058 0.0023 0.0003 0.0039 0.0003 0.0006
None 0.0062 0.0053 0.0069 0.0002 0.001 0.0045 0.0089 0.0049 0.0012 0.0001 0.04 0.0006 0.0063 0.0671 0.0015 0.0016 0.0002 0.0165
Nuclear Periphery 0.0436 0.0813 0.0774 0.0145 0.0299 0.017 0.093 0.046 0.0552 0.0046 0.0272 0.0188 0.0243 0.0746 0.0364 0.0249 0.0271 0.0201
Nucleolus 0.0964 0.1354 0.2569 0.4917 0.3285 0.1871 0.1221 0.162 0.3147 0.5305 0.3505 0.159 0.0941 0.207 0.3353 0.4394 0.3599 0.1703
Nucleus 0.7681 0.6483 0.4768 0.0868 0.0797 0.5798 0.54 0.6222 0.4134 0.1508 0.0708 0.5655 0.7139 0.5375 0.4778 0.076 0.143 0.6026
Peroxisomes 0.0001 0.0001 0.0003 0.0004 0.0055 0.0002 0.0085 0.0004 0.0088 0.001 0.0064 0.0054 0.0056 0.0018 0.0004 0.0093 0.0011 0.0011
Punctate Nuclear 0.0702 0.1042 0.1543 0.3775 0.2719 0.1945 0.1283 0.1386 0.0923 0.287 0.3763 0.1897 0.0792 0.0658 0.127 0.3428 0.4213 0.1662
Vacuole 0.001 0.0025 0.002 0.0018 0.0014 0.0009 0.0021 0.0021 0.0024 0.0005 0.0017 0.0012 0.001 0.0031 0.0012 0.0027 0.001 0.0014
Vacuole Periphery 0.0005 0.0009 0.0009 0.0004 0.0008 0.0003 0.0024 0.0009 0.0016 0.0001 0.0015 0.001 0.0008 0.0013 0.0005 0.0037 0.0002 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.4992 16.2022 12.4373 15.3006 10.9393 14.9912 20.2656 18.2055 14.4288 22.6443
Translational Efficiency 0.4913 0.7031 0.5723 0.4625 0.7435 0.7186 0.4935 0.3462 0.441 0.4267

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
848 19 2257 1406 1574 1110 438 102 2422 1129 2695 1508

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 701.13 315.17 1076.96 851.15 697.57 784.89 966.04 878.38 698.82 776.99 1058.93 852.99
Standard Deviation 390.97 6.82 142.20 104.40 106.14 99.70 111.02 96.95 246.66 115.86 143.57 104.14
Intensity Change Log 2 -1.153552 0.619211 0.279731 0.170152 0.469745 0.332507 -0.346504 0.546603 0.306293

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000448 0.000040 0.000584 0.000845 0.000385 0.000736 0.000799 0.001115 0.000407 0.000724 0.000619 0.000863
Bud Neck 0.003998 0.000018 0.028087 0.021009 0.006726 0.005457 0.006985 0.006394 0.005771 0.005366 0.024658 0.020021
Bud Site 0.002273 0.000031 0.003215 0.012015 0.000975 0.002045 0.001456 0.007513 0.001429 0.002011 0.002929 0.011710
Cell Periphery 0.000171 0.000069 0.000063 0.000314 0.000103 0.000076 0.000064 0.000161 0.000127 0.000076 0.000063 0.000303
Cytoplasm 0.016027 0.000039 0.016516 0.109430* 0.014600 0.008678 0.006216 0.040424 0.015100 0.008533 0.014842 0.104762*
Cytoplasmic Foci 0.007956 0.000020 0.000132 0.009068 0.003629 0.007555 0.001210 0.015835 0.005144 0.007428 0.000307 0.009526
Eisosomes 0.000072 0.000052 0.000017 0.000058 0.000049 0.000060 0.000064 0.000100 0.000057 0.000060 0.000025 0.000061
Endoplasmic Reticulum 0.002150 0.000001 0.003545 0.004920 0.002629 0.004890 0.004715 0.005609 0.002461 0.004807 0.003735 0.004966
Endosome 0.003561 0.000002 0.000487 0.012135 0.001790 0.005954 0.000615 0.007447 0.002410 0.005854 0.000508 0.011818
Golgi 0.002709 0.000005 0.000193 0.006253 0.001030 0.002814 0.000319 0.011532 0.001618 0.002767 0.000214 0.006610
Lipid Particles 0.004119 0.000040 0.000136 0.001402 0.002042 0.007057 0.000810 0.002894 0.002769 0.006939 0.000245 0.001503
Mitochondria 0.003542 0.000011 0.002184 0.014072 0.006910 0.012241 0.001232 0.006370 0.005731 0.012036 0.002029 0.013551
Mitotic Spindle 0.015037 0.000433 0.029265 0.215100* 0.013932 0.051681 0.035730 0.171549* 0.014319 0.050819 0.030316 0.212155*
None 0.027747 0.999044* 0.004056 0.013358 0.018089 0.005928 0.006506 0.006222 0.021471 0.022641 0.004454 0.012875
Nuclear Periphery 0.000921 0.000000 0.001566 0.004012 0.004579 0.008718 0.001300 0.001851 0.003298 0.008571 0.001523 0.003866
Nuclear Periphery Foci 0.041005 0.000058 0.003141 0.003436 0.034917 0.028630 0.007826 0.003530 0.037048 0.028149 0.003902 0.003443
Nucleolus 0.449098* 0.000039 0.074284 0.082883 0.358031* 0.286360* 0.179642 0.200617 0.389916* 0.281542* 0.091407 0.090846
Nucleus 0.411464* 0.000012 0.828647* 0.452658* 0.526354* 0.548384* 0.737709* 0.482852* 0.486128* 0.539155* 0.813867* 0.454700*
Peroxisomes 0.006369 0.000084 0.000263 0.001657 0.002062 0.006547 0.001175 0.004218 0.003570 0.006438 0.000411 0.001830
Vacuole 0.000888 0.000002 0.003347 0.028746 0.000745 0.005171 0.004960 0.017357 0.000795 0.005084 0.003609 0.027976
Vacuole Periphery 0.000444 0.000000 0.000274 0.006627 0.000422 0.001016 0.000669 0.006410 0.000430 0.000999 0.000338 0.006613

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 11.23 -5.99 -10.77 -18.87 -5.96 -3.75 -8.58 -6.08 -4.85 -3.19 -3.72 -8.76 -12.49 -5.53 -6.03
Bud Neck 12.47 -26.04 -19.67 -22.49 3.48 1.63 -5.83 -3.83 -4.42 -0.29 0.14 -24.80 -18.55 -18.53 2.15
Bud Site 4.23 -3.34 -7.73 -10.44 -5.98 -1.40 -2.93 -3.33 -2.91 -2.23 -0.73 -6.18 -9.31 -8.00 -6.37
Cell Periphery 2.68 3.89 0.18 -2.09 -2.69 2.20 2.04 -2.04 -3.49 -3.40 3.77 4.99 -0.90 -2.44 -2.80
Cytoplasm 7.21 1.15 -10.82 -16.32 -12.65 2.78 5.15 -2.03 -2.55 -2.91 3.31 2.32 -11.99 -13.41 -13.20
Cytoplasmic Foci 6.59 6.48 3.84 -7.44 -7.13 -1.86 3.82 -0.39 0.35 -1.60 -0.19 8.84 3.04 2.78 -6.90
Eisosomes 2.60 17.82 1.65 -2.02 -16.56 -3.23 -4.99 -4.20 -3.49 -2.22 -0.18 14.65 -4.22 -3.42 -13.75
Endoplasmic Reticulum 15.54 -7.66 -14.36 -21.32 -9.81 -4.51 -7.10 -5.16 -3.90 -3.12 -4.86 -9.14 -14.88 -6.24 -9.75
Endosome 4.60 3.65 -2.54 -9.69 -8.51 -3.32 2.78 -1.85 0.53 -2.34 -2.74 5.60 -5.18 -0.32 -8.69
Golgi 7.21 6.45 2.11 -4.38 -3.60 -2.56 3.48 -0.90 -0.49 -1.07 -1.20 8.17 -0.31 0.73 -3.60
Lipid Particles 3.44 3.32 2.20 -9.74 -8.74 -4.16 3.52 0.06 3.91 -2.00 -3.41 5.69 2.89 4.81 -8.20
Mitochondria 6.79 1.22 -2.51 -6.13 -3.38 -2.33 4.05 1.26 3.30 -1.98 -3.13 3.57 -0.45 2.74 -3.67
Mitotic Spindle 6.46 -2.63 -18.95 -23.02* -18.06 -6.85 -5.20 -4.87 -3.48 -3.26 -6.75 -5.58 -20.66 -13.78 -17.94
None -351.20* 9.40 7.33 1180.78* -5.89 6.94 5.70 5.70 -0.59 -0.38 -0.05 12.18 8.30 3.52 -5.49
Nuclear Periphery 7.36 -8.89 -14.21 -17.22 -10.44 -3.52 3.33 1.23 4.12 -1.70 -4.84 2.66 -6.87 1.74 -10.48
Nuclear Periphery Foci 12.48* 11.55 10.90 -9.49 -3.04 1.44 6.59 9.76 7.72 2.58 3.46 15.63 15.15 8.56 -1.28
Nucleolus 46.46* 29.98* 29.45* -22.33* 0.80 5.75 8.92 8.00 5.23 2.28 9.97 33.43* 33.40* 16.71 2.71
Nucleus 33.49* -31.94 -8.00 -49.67 24.75 -1.28 -7.08 -0.07 0.42 3.51 -4.72 -31.29 -2.04 2.55 23.86
Peroxisomes 6.86 6.53 5.39 -7.61 -5.75 -3.66 1.55 -0.36 2.71 -1.00 -2.18 8.32 5.60 4.53 -4.90
Vacuole 4.60 -6.28 -13.53 -13.81 -12.06 -3.98 -3.64 -3.94 -3.43 -3.14 -3.96 -7.43 -14.08 -11.85 -12.36
Vacuole Periphery 3.25 0.48 -3.38 -4.28 -3.59 -3.70 -0.50 -1.09 -0.83 -1.02 -3.69 0.52 -3.60 -2.39 -3.68