Standard name
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0.06 0.08 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0.09 0.05 0 0.08 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.97 0.98 0.98 0.95 0.85 0.75 0.75 0.74 0.66 0.62 0.63 0.98 0.99 0.99 0.98 0.96 0.98 0.84 0.96 0.95 0.92 0.9 0.92
Endoplasmic Reticulum – 0 0 0 0 0.06 0.17 0.17 0.11 0.18 0.19 0.17 0 0 0 0 0 0 0.06 0 0 0 0.06 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0.12 0 0.13 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 1 2 12 16 8 17 14 31 0 0 0 1 0 0 0 0 0 0 0 1
Bud – 1 0 1 1 0 0 0 0 2 2 1 1 1 2 0 0 1 0 0 0 0 3 6
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 1 5 1 28 18 5 16 24 35 2 5 4 3 4 6 0 0 0 0 0 0
Cytoplasm – 103 47 130 201 97 223 273 92 139 163 201 191 405 447 363 357 417 22 114 67 275 405 437
Endoplasmic Reticulum – 2 1 1 7 7 51 63 14 39 50 55 5 4 7 13 16 14 1 1 1 9 25 18
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi – 0 1 0 1 1 13 15 5 11 18 23 0 0 0 2 2 1 0 0 0 1 0 0
Mitochondria – 0 0 0 0 5 0 6 15 4 34 33 0 0 0 1 1 0 0 0 0 2 2 0
Nucleus – 0 0 0 0 0 0 2 1 1 3 0 0 0 0 0 0 0 2 0 0 1 1 1
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 1 4 2 5 4 6 2 11 0 2 0 3 8 1 0 2 0 6 5 4
Unique Cell Count 106 48 133 212 114 299 366 124 211 262 320 195 411 453 372 370 425 27 120 71 300 451 477
Labelled Cell Count 106 49 133 217 117 329 398 144 235 310 391 199 417 460 386 388 441 27 120 71 300 451 477


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 92.8 76.2 79.0 60.3 31.5 36.5 32.0 23.9 22.7 20.7 19.0 84.8 88.3 85.9 85.3 82.9 93.8 75.2 81.7 85.1
Std Deviation (1e-4) 1.6 19.7 19.7 21.1 14.9 12.2 9.1 7.9 6.7 6.7 6.6 5.6 19.7 25.5 23.0 26.9 26.3 31.3 19.2 21.4 23.7
Intensity Change (Log2) – – – 0.05 -0.34 -1.28 -1.06 -1.25 -1.67 -1.75 -1.88 -2.01 0.15 0.21 0.17 0.16 0.12 0.3 -0.02 0.1 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -0.9 -2.4 -3.6 -3.6 -3.5 -4.5 -4.9 -4.8 0 0.3 0.4 -0.1 -0.5 0.1
Endoplasmic Reticulum 0 0 0 2.7 2.7 0 2.8 2.9 2.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.6 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0