Standard name
Description Beta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; human homolog EIF2B2 can complement yeast mutant, allows growth down-regulation of yeast gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.13 0.07 0.08 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.08 0.06 0.11 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.38 0.64 0.55 0.56 0.78 0.76 0.68 0.59 0.52 0.57 0.47 0.51 0.64 0.19 0.24 0.34 0.45 0.36 0.35 0.59 0.43 0.54 0.47 0.48
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.1 0.05 0.09 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0.26 0 0.09 0.06 0 0 0 0 0 0 0 0 0.06 0.11 0.07 0.1 0.07 0.07 0.1 0 0.05 0.09 0.08 0.09
Golgi 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.12 0.09 0.29 0.35 0.25 0.53 0.38 0.5 0.47 0 0 0 0.11 0 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.19 0.1 0.05 0 0 0 0 0 0 0 0 0.07 0.3 0.33 0.26 0.19 0.24 0 0 0 0 0 0
Peroxisomes 0.1 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0.11 0.12 0 0 0.05 0 0 0 0 0 0
SpindlePole 0.16 0 0.21 0.17 0 0 0 0 0 0 0 0 0.2 0.53 0.46 0.23 0.2 0.19 0.29 0.12 0.18 0.14 0.22 0.2
Vac/Vac Membrane 0 0.09 0 0.08 0.13 0 0 0.1 0.05 0.05 0.06 0.09 0 0 0 0.14 0.15 0.2 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 2 7 13 1 2 8 12 9 18 12 5 16 5 0 5 7 11 5 3 2 5 11 13
Bud 0 1 6 13 0 4 2 5 6 4 9 11 3 0 2 0 3 4 17 9 27 20 46 52
Bud Neck 3 1 2 6 0 0 0 0 0 0 0 0 1 25 18 3 13 2 4 4 7 7 6 9
Bud Site 4 0 2 4 0 0 0 0 0 0 0 0 1 4 2 3 3 8 – – – – – –
Cell Periphery 0 0 7 11 11 11 23 39 16 38 28 32 6 1 0 1 5 4 0 0 0 0 0 0
Cytoplasm 51 45 101 140 255 248 349 302 148 193 159 208 210 94 123 78 149 110 55 60 87 155 264 291
Endoplasmic Reticulum 1 1 2 1 5 8 27 50 14 31 21 7 7 0 0 8 7 8 2 0 10 5 12 10
Endosome 35 1 16 14 0 1 3 3 0 0 2 7 18 56 34 23 23 23 15 2 11 27 46 56
Golgi 1 4 3 5 6 1 1 8 1 0 3 1 10 2 2 7 13 10 3 0 2 2 10 9
Mitochondria 12 2 7 31 30 95 179 129 149 126 169 190 3 1 4 26 6 20 0 0 2 1 2 1
Nucleus 0 0 0 0 1 0 1 3 0 2 1 0 0 4 10 3 1 1 0 0 0 2 2 4
Nuclear Periphery 0 0 0 0 0 0 1 2 0 1 0 2 0 0 0 0 0 0 0 1 0 0 0 1
Nucleolus 19 13 19 13 1 1 1 1 0 1 0 0 23 151 171 60 62 75 2 4 5 5 11 6
Peroxisomes 13 0 5 13 0 0 0 0 0 0 0 0 20 55 63 5 8 15 1 0 0 2 5 5
SpindlePole 22 2 39 41 2 0 0 0 1 0 0 1 67 270 238 52 67 59 45 12 36 41 127 119
Vac/Vac Membrane 6 6 7 19 44 10 17 53 14 17 19 36 8 14 9 33 51 60 0 1 3 7 12 15
Unique Cell Count 135 70 183 248 327 327 512 513 282 336 336 404 327 505 518 228 333 307 157 103 205 289 567 607
Labelled Cell Count 173 78 223 324 356 381 612 607 358 431 423 500 393 682 676 307 418 410 157 103 205 289 567 607


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.5 16.3 13.4 13.6 14.9 9.9 9.2 9.7 8.5 9.0 7.9 7.8 16.7 16.5 16.0 18.1 19.0 17.7 16.8 17.7 17.7
Std Deviation (1e-4) 2.7 3.9 3.0 4.0 7.8 2.0 1.9 2.4 2.2 2.9 1.8 1.8 3.5 4.5 5.8 7.7 7.6 7.3 3.3 3.9 4.3
Intensity Change (Log2) – – – 0.03 0.16 -0.43 -0.54 -0.46 -0.65 -0.57 -0.75 -0.77 0.32 0.31 0.26 0.44 0.51 0.4 0.33 0.4 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.7 0 0 0 0 -0.4 0.8 -0.1 0 0.6 0 0 -1.0 0 -0.1
Bud 1.0 0 0 0 0 0 0 -0.4 -0.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 2.3 1.7 0 1.8 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.3 -0.3 -0.3 0.4 1.8 0.9 2.9 2.0 1.8 0 0 0 0 0 0
Cytoplasm 0.3 5.4 4.8 3.2 0.9 -0.6 0.5 -1.7 -0.8 2.0 -9.4 -7.8 -4.3 -2.3 -4.2
Endoplasmic Reticulum 0 0 0 2.4 3.8 2.2 3.6 2.7 0 0 0 0 0 0 0
Endosome -1.2 -5.4 -5.1 -5.8 0 -5.1 -5.5 -4.8 -4.1 -1.4 0.9 -1.0 0.5 -0.8 -0.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0
Mitochondria 3.1 2.2 6.8 8.2 6.2 10.9 8.4 10.7 10.3 0 0 0 2.8 0 1.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.0 -5.6 -5.6 -7.1 -7.1 -5.5 -5.7 -6.0 -6.6 -1.3 5.2 5.9 4.1 2.5 3.8
Peroxisomes 1.3 0 0 0 0 0 0 0 0 1.7 3.4 3.7 0 0 1.2
SpindlePole -1.3 -8.2 -8.7 -10.8 -10.8 -7.9 -8.8 -8.8 -9.4 -0.2 7.5 5.9 0.4 -0.3 -0.6
Vacuole 1.7 3.5 -0.5 -0.3 2.7 0.6 0.6 0.9 2.2 -0.9 -0.7 0 3.6 4.0 4.9