Standard name
Human Ortholog
Description Beta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; human homolog EIF2B2 can complement yeast mutant, allows growth down-regulation of yeast gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.13 0.07 0.08 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.08 0.06 0.11 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.38 0.64 0.55 0.56 0.78 0.76 0.68 0.59 0.52 0.57 0.47 0.51 0.64 0.19 0.24 0.34 0.45 0.36 0.35 0.59 0.43 0.54 0.47 0.48
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.1 0.05 0.09 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0.26 0 0.09 0.06 0 0 0 0 0 0 0 0 0.06 0.11 0.07 0.1 0.07 0.07 0.1 0 0.05 0.09 0.08 0.09
Golgi 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.12 0.09 0.29 0.35 0.25 0.53 0.38 0.5 0.47 0 0 0 0.11 0 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.19 0.1 0.05 0 0 0 0 0 0 0 0 0.07 0.3 0.33 0.26 0.19 0.24 0 0 0 0 0 0
Peroxisomes 0.1 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0.11 0.12 0 0 0.05 0 0 0 0 0 0
SpindlePole 0.16 0 0.21 0.17 0 0 0 0 0 0 0 0 0.2 0.53 0.46 0.23 0.2 0.19 0.29 0.12 0.18 0.14 0.22 0.2
Vac/Vac Membrane 0 0.09 0 0.08 0.13 0 0 0.1 0.05 0.05 0.06 0.09 0 0 0 0.14 0.15 0.2 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 2 7 13 1 2 8 12 9 18 12 5 16 5 0 5 7 11 5 3 2 5 11 13
Bud 0 1 6 13 0 4 2 5 6 4 9 11 3 0 2 0 3 4 17 9 27 20 46 52
Bud Neck 3 1 2 6 0 0 0 0 0 0 0 0 1 25 18 3 13 2 4 4 7 7 6 9
Bud Site 4 0 2 4 0 0 0 0 0 0 0 0 1 4 2 3 3 8 – – – – – –
Cell Periphery 0 0 7 11 11 11 23 39 16 38 28 32 6 1 0 1 5 4 0 0 0 0 0 0
Cytoplasm 51 45 101 140 255 248 349 302 148 193 159 208 210 94 123 78 149 110 55 60 87 155 264 291
Endoplasmic Reticulum 1 1 2 1 5 8 27 50 14 31 21 7 7 0 0 8 7 8 2 0 10 5 12 10
Endosome 35 1 16 14 0 1 3 3 0 0 2 7 18 56 34 23 23 23 15 2 11 27 46 56
Golgi 1 4 3 5 6 1 1 8 1 0 3 1 10 2 2 7 13 10 3 0 2 2 10 9
Mitochondria 12 2 7 31 30 95 179 129 149 126 169 190 3 1 4 26 6 20 0 0 2 1 2 1
Nucleus 0 0 0 0 1 0 1 3 0 2 1 0 0 4 10 3 1 1 0 0 0 2 2 4
Nuclear Periphery 0 0 0 0 0 0 1 2 0 1 0 2 0 0 0 0 0 0 0 1 0 0 0 1
Nucleolus 19 13 19 13 1 1 1 1 0 1 0 0 23 151 171 60 62 75 2 4 5 5 11 6
Peroxisomes 13 0 5 13 0 0 0 0 0 0 0 0 20 55 63 5 8 15 1 0 0 2 5 5
SpindlePole 22 2 39 41 2 0 0 0 1 0 0 1 67 270 238 52 67 59 45 12 36 41 127 119
Vac/Vac Membrane 6 6 7 19 44 10 17 53 14 17 19 36 8 14 9 33 51 60 0 1 3 7 12 15
Unique Cell Count 135 70 183 248 327 327 512 513 282 336 336 404 327 505 518 228 333 307 157 103 205 289 567 607
Labelled Cell Count 173 78 223 324 356 381 612 607 358 431 423 500 393 682 676 307 418 410 157 103 205 289 567 607


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.5 16.3 13.4 13.6 14.9 9.9 9.2 9.7 8.5 9.0 7.9 7.8 16.7 16.5 16.0 18.1 19.0 17.7 16.8 17.7 17.7
Std Deviation (1e-4) 2.7 3.9 3.0 4.0 7.8 2.0 1.9 2.4 2.2 2.9 1.8 1.8 3.5 4.5 5.8 7.7 7.6 7.3 3.3 3.9 4.3
Intensity Change (Log2) – – – 0.03 0.16 -0.43 -0.54 -0.46 -0.65 -0.57 -0.75 -0.77 0.32 0.31 0.26 0.44 0.51 0.4 0.33 0.4 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.7 0 0 0 0 -0.4 0.8 -0.1 0 0.6 0 0 -1.0 0 -0.1
Bud 1.0 0 0 0 0 0 0 -0.4 -0.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 2.3 1.7 0 1.8 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.3 -0.3 -0.3 0.4 1.8 0.9 2.9 2.0 1.8 0 0 0 0 0 0
Cytoplasm 0.3 5.4 4.8 3.2 0.9 -0.6 0.5 -1.7 -0.8 2.0 -9.4 -7.8 -4.3 -2.3 -4.2
Endoplasmic Reticulum 0 0 0 2.4 3.8 2.2 3.6 2.7 0 0 0 0 0 0 0
Endosome -1.2 -5.4 -5.1 -5.8 0 -5.1 -5.5 -4.8 -4.1 -1.4 0.9 -1.0 0.5 -0.8 -0.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0
Mitochondria 3.1 2.2 6.8 8.2 6.2 10.9 8.4 10.7 10.3 0 0 0 2.8 0 1.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.0 -5.6 -5.6 -7.1 -7.1 -5.5 -5.7 -6.0 -6.6 -1.3 5.2 5.9 4.1 2.5 3.8
Peroxisomes 1.3 0 0 0 0 0 0 0 0 1.7 3.4 3.7 0 0 1.2
SpindlePole -1.3 -8.2 -8.7 -10.8 -10.8 -7.9 -8.8 -8.8 -9.4 -0.2 7.5 5.9 0.4 -0.3 -0.6
Vacuole 1.7 3.5 -0.5 -0.3 2.7 0.6 0.6 0.9 2.2 -0.9 -0.7 0 3.6 4.0 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.6942 17.3015 14.8976 12.9032 15.0762 14.241 7.7419 18.3664 13.6109 11.0302 13.4612 13.8542 6.4025 15.3178 12.5494 10.4912 9.4505 12.2565
Actin 0.0088 0.0064 0.0088 0.0005 0 0.0043 0.0142 0.0018 0.0108 0.0566 0.0006 0.0069 0.0172 0.0035 0.0072 0.0009 0.0011 0.0013
Bud 0.0089 0.0207 0.0133 0.001 0.0079 0.01 0.0041 0.0217 0.0222 0.0221 0.0361 0.015 0.0099 0.0179 0.0209 0.0004 0.0026 0.0101
Bud Neck 0.0087 0.0027 0.0044 0.0021 0.0038 0.0138 0.002 0.005 0.0071 0.0458 0.0037 0.0208 0.0062 0.0053 0.0045 0.0008 0.0027 0.0158
Bud Periphery 0.0053 0.0067 0.004 0.0005 0.003 0.0045 0.0021 0.0054 0.0091 0.0126 0.014 0.006 0.0034 0.0058 0.008 0.0002 0.0011 0.009
Bud Site 0.0255 0.0853 0.0235 0.0066 0.0172 0.0066 0.0156 0.101 0.0295 0.0337 0.0101 0.0101 0.019 0.0489 0.0498 0.0019 0.0109 0.0053
Cell Periphery 0.0012 0.002 0.0008 0.0007 0.0012 0.0005 0.0015 0.0033 0.0021 0.0024 0.0034 0.0028 0.0028 0.0032 0.0041 0.0005 0.0003 0.0019
Cytoplasm 0.0521 0.0911 0.1079 0.0046 0.0294 0.0429 0.0975 0.1137 0.1829 0.0966 0.0146 0.0634 0.0951 0.0823 0.0572 0.0461 0.0513 0.0731
Cytoplasmic Foci 0.6586 0.5669 0.6193 0.7132 0.6533 0.7174 0.5141 0.5125 0.4356 0.3403 0.565 0.498 0.5308 0.6198 0.5734 0.7691 0.6818 0.6647
Eisosomes 0.0001 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0001 0.0003 0 0.0001 0.0004 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0036 0.0008 0.0057 0.0012 0.0005 0.0022 0.0072 0.0004 0.0019 0.0042 0.0001 0.0173 0.0134 0.0002 0.0051 0.0002 0.003 0.0044
Endosome 0.0755 0.0629 0.0709 0.0443 0.0536 0.0776 0.0821 0.0345 0.0878 0.0886 0.1304 0.0986 0.0979 0.0323 0.049 0.0381 0.0703 0.0633
Golgi 0.0156 0.0085 0.0111 0.0078 0.0045 0.0192 0.0176 0.0078 0.0136 0.009 0.0413 0.0197 0.0136 0.0042 0.0137 0.0033 0.0086 0.0069
Lipid Particles 0.0448 0.0226 0.0372 0.0407 0.0324 0.0268 0.0327 0.0307 0.019 0.01 0.0186 0.0532 0.0534 0.0403 0.0404 0.0415 0.0641 0.0463
Mitochondria 0.0057 0.0034 0.0083 0.0025 0.0044 0.004 0.0302 0.0126 0.045 0.0574 0.0098 0.0192 0.0119 0.0122 0.0133 0.0008 0.0059 0.008
None 0.0035 0.0014 0.0024 0.0004 0.0003 0.0009 0.0076 0.0022 0.0009 0.0012 0.0003 0.001 0.0122 0.0011 0.0029 0.0002 0.0019 0.0006
Nuclear Periphery 0.001 0.0003 0.0029 0.0016 0.0002 0.0026 0.0206 0.0008 0.003 0.0036 0.0002 0.0029 0.0056 0.0003 0.0007 0.0001 0.0055 0.004
Nucleolus 0.0188 0.0245 0.0151 0.015 0.1139 0.0139 0.0336 0.0641 0.0507 0.0879 0.0591 0.0625 0.026 0.0398 0.0533 0.0634 0.0291 0.0152
Nucleus 0.0024 0.0027 0.0044 0.0029 0.0083 0.0051 0.0105 0.0062 0.0094 0.0148 0.0043 0.005 0.0088 0.0043 0.0103 0.0039 0.0087 0.0088
Peroxisomes 0.0342 0.0281 0.0199 0.0317 0.0257 0.02 0.0357 0.0242 0.015 0.0197 0.0068 0.0248 0.0282 0.0364 0.0309 0.0158 0.0261 0.0198
Punctate Nuclear 0.0097 0.0116 0.0079 0.0112 0.0174 0.011 0.0049 0.0051 0.0044 0.0062 0.0015 0.0078 0.015 0.0057 0.0036 0.0026 0.0131 0.0093
Vacuole 0.0142 0.0483 0.0295 0.1036 0.0219 0.0142 0.056 0.0431 0.0407 0.0722 0.0716 0.0544 0.0248 0.0342 0.0463 0.0094 0.0099 0.0285
Vacuole Periphery 0.0018 0.0029 0.0027 0.0077 0.0014 0.0022 0.01 0.0038 0.009 0.0149 0.0085 0.0105 0.0044 0.0023 0.0054 0.0008 0.0019 0.0038

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 62.1026 55.9463 54.2883 67.212 59.2967 45.228 49.17 49.0454 46.4503 71.2038
Translational Efficiency 0.885 0.9009 0.9145 0.7065 0.8901 1.2168 1.079 0.8426 1.039 0.9064

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Beta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; human homolog EIF2B2 can complement yeast mutant, allows growth down-regulation of yeast gene
Localization
Cell Percentages cytoplasm (74%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-8

Gcd7

Gcd7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcd7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available