Standard name | |
---|---|
Description | Gamma-glutamyltranspeptidase; major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 3 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 110 | 85 | – | 61 | 76 | 62 | 95 | 135 | 49 | 102 | 92 | 171 | 89 | 221 | 346 | 213 | 224 | 156 | 14 | 10 | 2 | 9 | 34 | 31 |
Endoplasmic Reticulum | 0 | 0 | – | 1 | 4 | 1 | 7 | 2 | 3 | 2 | 3 | 0 | 2 | 0 | 1 | 7 | 6 | 14 | 7 | 0 | 0 | 0 | 0 | 0 |
Endosome | 13 | 7 | – | 0 | 3 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 8 | 6 | 5 | 11 | 9 | 0 | 1 | 5 | 6 |
Golgi | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 25 | 1 | – | 0 | 1 | 2 | 11 | 6 | 5 | 10 | 13 | 9 | 0 | 0 | 1 | 8 | 9 | 4 | 2 | 3 | 0 | 0 | 4 | 5 |
Nucleus | 20 | 9 | – | 4 | 26 | 45 | 71 | 88 | 37 | 36 | 29 | 28 | 11 | 4 | 1 | 16 | 22 | 14 | 1 | 1 | 0 | 1 | 2 | 3 |
Nuclear Periphery | 4 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 2 |
Vac/Vac Membrane | 87 | 93 | – | 31 | 128 | 114 | 215 | 217 | 79 | 117 | 96 | 174 | 15 | 34 | 15 | 166 | 181 | 133 | 272 | 149 | 36 | 71 | 191 | 217 |
Unique Cell Count | 220 | 164 | 92 | 190 | 170 | 326 | 347 | 132 | 207 | 178 | 300 | 104 | 247 | 354 | 335 | 367 | 273 | 318 | 179 | 42 | 87 | 249 | 280 | |
Labelled Cell Count | 268 | 197 | 100 | 240 | 227 | 401 | 451 | 173 | 267 | 234 | 386 | 118 | 260 | 364 | 422 | 454 | 329 | 318 | 179 | 42 | 87 | 249 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.3 | – | 6.1 | 7.9 | 7.2 | 7.1 | 7.3 | 5.8 | 6.1 | 5.6 | 6.0 | 5.6 | 5.6 | 5.2 | 6.5 | 6.4 | 7.6 | 4.7 | 5.6 | 5.9 |
Std Deviation (1e-4) | 0.7 | 0.9 | – | 1.6 | 1.6 | 1.5 | 1.5 | 1.7 | 1.4 | 1.5 | 1.4 | 1.4 | 1.1 | 1.6 | 1.1 | 1.4 | 1.6 | 4.8 | 0.7 | 1.3 | 2.1 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |