Standard name
Description Major exo-1,3-beta-glucanase of the cell wall; involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; EXG1 has a paralog, SPR1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.23 0.37 0.57 0.23 0.18 0.24 0.25 0.36 0.33 0.54 0.41 0.68 0.84 0.92 0.23 0.19 0.2 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.49 0.14 0.09 0.07 0.06 0.09 0.06 0 0 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0.14 0.25 0.1 0.22 0.32 0.41 0.33 0.23 0.25 0.11 0.15 0.09 0 0 0.47 0.41 0.41 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.73 0.57 0.45 0.72 0.61 0.59 0.68 0.69 0.66 0.62 0.58 0.29 0.2 0.1 0.47 0.54 0.48 0.86 0.84 0.88 0.85 0.86 0.83
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 1 0 0 0
Bud 1 0 0 0 0 0 0 2 0 5 1 0 1 0 0 0 0 0 2 2 2 4 3 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 3 0 0 0 0 0 0 0 0 0
Cytoplasm 45 25 64 60 40 20 60 88 146 168 115 128 138 245 326 42 40 37 6 2 3 11 12 12
Endoplasmic Reticulum 1 0 1 0 4 1 2 0 3 2 3 1 7 1 1 10 7 11 0 0 1 0 0 1
Endosome 0 0 3 4 1 0 1 0 0 0 0 0 3 2 0 3 9 4 7 7 5 1 8 19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 1 0 0 2
Mitochondria 90 16 15 7 10 10 14 13 18 39 18 11 0 2 1 3 4 5 2 2 3 3 6 7
Nucleus 18 16 43 11 38 36 102 114 94 130 23 47 19 6 6 86 89 76 1 3 0 2 4 6
Nuclear Periphery 1 0 0 0 3 3 1 1 3 1 0 3 0 0 0 1 1 0 1 0 0 0 2 3
Nucleolus 0 0 0 0 3 0 1 0 2 1 0 2 0 0 0 0 3 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 0 0 1 1 0 1 1 0 1 1 0 0 1 0 1 0 1 0 2 2
Vac/Vac Membrane 104 81 99 48 125 69 147 239 275 337 132 180 59 59 35 86 116 88 168 106 173 158 275 296
Unique Cell Count 185 111 174 106 174 113 251 350 401 514 214 312 202 291 355 183 215 184 195 127 197 186 321 359
Labelled Cell Count 261 138 225 131 226 139 329 458 541 685 295 373 228 318 375 232 271 221 195 127 197 186 321 359


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.7 5.1 5.3 6.8 7.3 7.3 7.0 7.1 7.0 6.6 6.5 5.9 5.7 5.3 7.2 7.2 6.9 6.6 6.6 6.8
Std Deviation (1e-4) 0.8 1.3 1.3 2.3 1.8 2.3 2.5 2.3 2.4 2.2 2.2 2.1 1.1 1.2 1.5 1.5 1.8 1.9 1.7 1.6 1.8
Intensity Change (Log2) – – – 0.07 0.42 0.53 0.52 0.48 0.48 0.47 0.39 0.36 0.23 0.16 0.08 0.5 0.5 0.44 0.38 0.39 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 -2.8 -3.5 -2.9 -2.8 -0.1 -1.0 3.3 0.9 6.1 10.5 13.5 -2.9 -4.0 -3.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.6 -1.0 0.1 -1.2 -2.4 -2.0 -0.4 -0.1 -2.4 -4.3 -4.4 -5.3 -3.0 -3.1 -2.4
Nucleus -2.9 -0.6 1.3 3.4 1.8 -0.3 0.2 -3.6 -2.6 -4.0 -7.7 -8.6 4.4 3.5 3.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 2.9 0.7 0.3 2.6 2.7 2.1 1.0 0.2 -5.4 -8.1 -11.7 -1.9 -0.6 -1.7