Standard name
Human Ortholog
Description Major exo-1,3-beta-glucanase of the cell wall; involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; EXG1 has a paralog, SPR1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.23 0.37 0.57 0.23 0.18 0.24 0.25 0.36 0.33 0.54 0.41 0.68 0.84 0.92 0.23 0.19 0.2 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.49 0.14 0.09 0.07 0.06 0.09 0.06 0 0 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0.14 0.25 0.1 0.22 0.32 0.41 0.33 0.23 0.25 0.11 0.15 0.09 0 0 0.47 0.41 0.41 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.73 0.57 0.45 0.72 0.61 0.59 0.68 0.69 0.66 0.62 0.58 0.29 0.2 0.1 0.47 0.54 0.48 0.86 0.84 0.88 0.85 0.86 0.83
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 1 0 0 0
Bud 1 0 0 0 0 0 0 2 0 5 1 0 1 0 0 0 0 0 2 2 2 4 3 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 3 0 0 0 0 0 0 0 0 0
Cytoplasm 45 25 64 60 40 20 60 88 146 168 115 128 138 245 326 42 40 37 6 2 3 11 12 12
Endoplasmic Reticulum 1 0 1 0 4 1 2 0 3 2 3 1 7 1 1 10 7 11 0 0 1 0 0 1
Endosome 0 0 3 4 1 0 1 0 0 0 0 0 3 2 0 3 9 4 7 7 5 1 8 19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 1 0 0 2
Mitochondria 90 16 15 7 10 10 14 13 18 39 18 11 0 2 1 3 4 5 2 2 3 3 6 7
Nucleus 18 16 43 11 38 36 102 114 94 130 23 47 19 6 6 86 89 76 1 3 0 2 4 6
Nuclear Periphery 1 0 0 0 3 3 1 1 3 1 0 3 0 0 0 1 1 0 1 0 0 0 2 3
Nucleolus 0 0 0 0 3 0 1 0 2 1 0 2 0 0 0 0 3 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 0 0 1 1 0 1 1 0 1 1 0 0 1 0 1 0 1 0 2 2
Vac/Vac Membrane 104 81 99 48 125 69 147 239 275 337 132 180 59 59 35 86 116 88 168 106 173 158 275 296
Unique Cell Count 185 111 174 106 174 113 251 350 401 514 214 312 202 291 355 183 215 184 195 127 197 186 321 359
Labelled Cell Count 261 138 225 131 226 139 329 458 541 685 295 373 228 318 375 232 271 221 195 127 197 186 321 359


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.7 5.1 5.3 6.8 7.3 7.3 7.0 7.1 7.0 6.6 6.5 5.9 5.7 5.3 7.2 7.2 6.9 6.6 6.6 6.8
Std Deviation (1e-4) 0.8 1.3 1.3 2.3 1.8 2.3 2.5 2.3 2.4 2.2 2.2 2.1 1.1 1.2 1.5 1.5 1.8 1.9 1.7 1.6 1.8
Intensity Change (Log2) 0.07 0.42 0.53 0.52 0.48 0.48 0.47 0.39 0.36 0.23 0.16 0.08 0.5 0.5 0.44 0.38 0.39 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 -2.8 -3.5 -2.9 -2.8 -0.1 -1.0 3.3 0.9 6.1 10.5 13.5 -2.9 -4.0 -3.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.6 -1.0 0.1 -1.2 -2.4 -2.0 -0.4 -0.1 -2.4 -4.3 -4.4 -5.3 -3.0 -3.1 -2.4
Nucleus -2.9 -0.6 1.3 3.4 1.8 -0.3 0.2 -3.6 -2.6 -4.0 -7.7 -8.6 4.4 3.5 3.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 2.9 0.7 0.3 2.6 2.7 2.1 1.0 0.2 -5.4 -8.1 -11.7 -1.9 -0.6 -1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4242 3.2531 2.4866 2.0204 2.1704 2.7246 1.2938 2.3103 1.8234 2.7852 1.9796 2.1776 0.1792 1.7776 1.2295 1.5268 1.7651 1.5309
Actin 0.018 0.0001 0.0083 0 0.0068 0.0034 0.0284 0.0004 0.0007 0.0002 0.0062 0.0007 0.0094 0 0.0005 0 0.005 0.0005
Bud 0.0004 0.0005 0.0006 0.0003 0.0005 0.0008 0.0007 0.0007 0.0009 0.0003 0.0004 0.0005 0.0004 0.0004 0.0003 0.0003 0.0004 0.0003
Bud Neck 0.0013 0.0013 0.0012 0.0009 0.0014 0.002 0.0019 0.0025 0.0018 0.001 0.0018 0.0028 0.0009 0.0011 0.0011 0.0007 0.0008 0.0016
Bud Periphery 0.0008 0.001 0.0014 0.0009 0.0017 0.0026 0.0018 0.0016 0.0015 0.0008 0.0011 0.0016 0.0009 0.0009 0.0009 0.0008 0.0008 0.0009
Bud Site 0.0026 0.0038 0.0022 0.001 0.002 0.0018 0.0044 0.0047 0.0027 0.0008 0.0011 0.001 0.0007 0.0017 0.0016 0.0007 0.0012 0.0008
Cell Periphery 0.0016 0.0036 0.0025 0.001 0.0028 0.0037 0.0019 0.0033 0.0026 0.0016 0.0027 0.0033 0.0015 0.0032 0.0017 0.0017 0.001 0.0029
Cytoplasm 0.0134 0.0182 0.009 0.0032 0.0021 0.0087 0.0209 0.017 0.0096 0.0066 0.0096 0.0047 0.0078 0.009 0.0066 0.0008 0.0046 0.0063
Cytoplasmic Foci 0.0282 0.0071 0.015 0.0075 0.0176 0.0136 0.012 0.0091 0.0121 0.0026 0.0073 0.0069 0.013 0.0091 0.0097 0.002 0.0184 0.0075
Eisosomes 0.0003 0 0.0001 0 0.0001 0.0001 0.0003 0 0 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0046 0.0039 0.0025 0.0009 0.0017 0.0032 0.0098 0.0034 0.0025 0.0031 0.0052 0.0022 0.003 0.0019 0.0031 0.0006 0.0008 0.0017
Endosome 0.2032 0.1052 0.1918 0.2331 0.1437 0.1506 0.2293 0.128 0.2123 0.1357 0.1102 0.1347 0.2216 0.1135 0.2049 0.1658 0.1416 0.152
Golgi 0.0141 0.0013 0.0095 0.0065 0.0123 0.0072 0.0169 0.0023 0.0159 0.0031 0.0155 0.004 0.0088 0.0019 0.0057 0.0034 0.0032 0.003
Lipid Particles 0.0404 0.0033 0.0265 0.0055 0.0403 0.0211 0.0164 0.0098 0.0143 0.0058 0.0111 0.0081 0.0191 0.005 0.0114 0.0039 0.0183 0.0166
Mitochondria 0.0148 0.0013 0.0578 0.0117 0.0051 0.0149 0.0365 0.0112 0.0258 0.0482 0.0199 0.0034 0.0117 0.0053 0.0307 0.0022 0.0106 0.0022
None 0.0769 0.0113 0.0279 0.0038 0.0012 0.0051 0.0085 0.0079 0.0076 0.0001 0.0076 0.0053 0.0085 0.0086 0.0034 0.0001 0.0051 0.0005
Nuclear Periphery 0.013 0.0035 0.0042 0.0017 0.0035 0.0102 0.029 0.0056 0.0062 0.0029 0.0204 0.0095 0.0096 0.0105 0.0052 0.0014 0.0105 0.0095
Nucleolus 0.0035 0.0027 0.0068 0.0038 0.0029 0.0041 0.0044 0.0104 0.008 0.0026 0.0025 0.0057 0.0034 0.0068 0.0045 0.0026 0.0025 0.0048
Nucleus 0.0073 0.004 0.0056 0.0052 0.0038 0.0058 0.0139 0.0102 0.0149 0.0311 0.0079 0.0085 0.0052 0.0088 0.0076 0.0028 0.022 0.0124
Peroxisomes 0.0117 0.0002 0.0023 0.0011 0.0258 0.0045 0.0116 0.0007 0.0032 0.0008 0.0016 0.0031 0.003 0.0004 0.0015 0.0004 0.0092 0.0007
Punctate Nuclear 0.0085 0.0001 0.0006 0.0002 0.0045 0.0015 0.0039 0.0003 0.0017 0.0001 0.0009 0.0015 0.0009 0.0002 0.0005 0 0.0271 0.0032
Vacuole 0.4182 0.6895 0.4958 0.5987 0.6199 0.6159 0.4375 0.6624 0.5481 0.6367 0.6732 0.6729 0.5033 0.649 0.5557 0.6459 0.5663 0.6221
Vacuole Periphery 0.117 0.1381 0.1283 0.113 0.1001 0.1193 0.11 0.1084 0.1075 0.116 0.0934 0.1197 0.1672 0.1624 0.1433 0.1638 0.1506 0.1506

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 194.9594 341.2408 172.2517 391.9986 514.0432 153.9163 237.6696 160.1611 316.6733 487.0685
Translational Efficiency 1.257 1.2788 1.0772 1.1089 1.0526 1.2514 1.3001 1.0769 1.0838 1.149

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Major exo-1,3-beta-glucanase of the cell wall; involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; EXG1 has a paralog, SPR1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (94%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Exg1

Exg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Exg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available