Standard name | |
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Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 6 | 6 | 3 | 3 | 0 | 1 | 1 | 4 | 1 | 1 | 1 | 2 | 0 | 3 | 0 | 3 | 4 | 4 | 6 | 3 | 4 | 5 |
Bud | 0 | 9 | 3 | 6 | 5 | 4 | 12 | 6 | 10 | 12 | 16 | 22 | 2 | 1 | 0 | 9 | 2 | 6 | 0 | 1 | 2 | 5 | 9 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 3 | 3 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 10 | 18 | 3 | 2 | 8 | 1 | 4 | 3 | 6 | 4 | 2 | 4 | 1 | 2 | 20 | 17 | 19 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 7 | 7 | 6 | 14 | 9 | 20 | 18 | 5 | 5 | 3 | 8 | 3 | 7 | 6 | 21 | 22 | 18 | 0 | 1 | 0 | 9 | 11 | 6 |
Endoplasmic Reticulum | 0 | 9 | 1 | 2 | 3 | 2 | 1 | 2 | 9 | 5 | 2 | 6 | 0 | 4 | 3 | 3 | 6 | 4 | 0 | 0 | 2 | 0 | 2 | 1 |
Endosome | 0 | 2 | 15 | 5 | 3 | 26 | 3 | 52 | 3 | 14 | 11 | 5 | 4 | 10 | 5 | 8 | 7 | 15 | 4 | 6 | 10 | 8 | 7 | 8 |
Golgi | 5 | 1 | 80 | 45 | 15 | 20 | 1 | 13 | 0 | 10 | 2 | 7 | 25 | 66 | 64 | 13 | 40 | 42 | 4 | 21 | 19 | 10 | 30 | 18 |
Mitochondria | 147 | 275 | 293 | 252 | 268 | 429 | 475 | 444 | 485 | 619 | 495 | 604 | 122 | 167 | 145 | 6 | 7 | 15 | 127 | 234 | 361 | 133 | 159 | 163 |
Nucleus | 0 | 1 | 3 | 0 | 5 | 2 | 15 | 6 | 3 | 3 | 2 | 8 | 3 | 2 | 2 | 3 | 1 | 5 | 0 | 1 | 0 | 6 | 13 | 6 |
Nuclear Periphery | 11 | 7 | 14 | 9 | 14 | 42 | 44 | 67 | 39 | 67 | 42 | 70 | 10 | 17 | 8 | 4 | 8 | 9 | 1 | 2 | 2 | 1 | 3 | 3 |
Nucleolus | 12 | 6 | 7 | 13 | 18 | 38 | 54 | 48 | 35 | 56 | 32 | 36 | 4 | 1 | 3 | 8 | 3 | 16 | 0 | 0 | 1 | 1 | 3 | 4 |
Peroxisomes | 3 | 1 | 20 | 11 | 4 | 10 | 2 | 16 | 2 | 11 | 6 | 3 | 5 | 0 | 5 | 26 | 16 | 27 | 7 | 6 | 32 | 5 | 11 | 14 |
SpindlePole | 0 | 0 | 3 | 1 | 2 | 10 | 10 | 20 | 4 | 9 | 8 | 11 | 2 | 0 | 0 | 1 | 0 | 11 | 0 | 0 | 1 | 2 | 3 | 5 |
Vac/Vac Membrane | 14 | 39 | 45 | 12 | 22 | 53 | 51 | 53 | 26 | 40 | 39 | 37 | 13 | 20 | 29 | 30 | 34 | 50 | 2 | 16 | 4 | 12 | 19 | 4 |
Unique Cell Count | 152 | 296 | 377 | 288 | 309 | 530 | 545 | 616 | 515 | 697 | 540 | 667 | 144 | 211 | 194 | 132 | 140 | 192 | 153 | 301 | 446 | 206 | 286 | 257 |
Labelled Cell Count | 193 | 367 | 515 | 371 | 379 | 661 | 692 | 754 | 628 | 862 | 663 | 825 | 198 | 298 | 272 | 155 | 164 | 240 | 153 | 301 | 446 | 206 | 286 | 257 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.9 | 7.4 | 10.3 | 9.9 | 7.3 | 8.2 | 5.3 | 8.1 | 7.0 | 7.6 | 7.4 | 6.9 | 9.0 | 9.2 | 9.0 | 9.0 | 11.1 | 11.3 | 10.2 | 10.2 | 10.6 |
Std Deviation (1e-4) | 2.0 | 1.6 | 1.8 | 1.8 | 1.7 | 1.6 | 1.4 | 1.6 | 1.5 | 1.6 | 1.7 | 1.6 | 1.8 | 1.4 | 1.6 | 2.4 | 2.8 | 3.2 | 2.6 | 2.9 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.5 | -0.33 | -0.95 | -0.34 | -0.55 | -0.45 | -0.48 | -0.59 | -0.2 | -0.17 | -0.2 | -0.19 | 0.11 | 0.13 | -0.01 | -0.01 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1.7 | 0 | 1.4 | 1.2 | 2.3 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.7 | -3.2 | -2.9 | -4.8 | -4.3 | -4.1 | -4.1 | -3.9 | -5.1 | -1.0 | -2.8 | -2.3 | 3.9 | 3.0 | 2.3 |
Cytoplasm | 0.2 | 2.0 | -0.2 | 1.6 | 1.0 | -1.1 | 0 | 0 | -0.9 | 0 | 1.1 | 0 | 6.1 | 6.1 | 4.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.7 | -2.5 | 0.7 | -3.7 | 2.7 | -3.6 | -1.9 | -1.7 | -3.7 | -0.7 | 0.4 | -0.9 | 1.0 | 0.5 | 1.9 |
Golgi | -1.8 | -6.2 | -8.3 | -11.1 | -10.0 | -11.0 | -11.2 | -10.9 | -11.3 | -1.0 | 2.7 | 3.1 | -2.9 | 1.8 | 0.2 |
Mitochondria | 3.2 | 3.0 | 1.2 | 3.8 | -2.0 | 7.3 | 4.8 | 6.0 | 5.7 | 1.8 | 0.4 | -0.8 | -14.7 | -14.9 | -15.8 |
Nucleus | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.4 | 0.5 | 2.6 | 2.7 | 4.0 | 2.4 | 3.5 | 2.5 | 3.9 | 1.6 | 2.3 | 0.2 | 0 | 1.0 | 0.6 |
Nucleolus | 2.0 | 2.8 | 3.6 | 4.8 | 4.0 | 3.4 | 4.1 | 3.0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 3.7 |
Peroxisomes | -0.9 | -2.8 | -2.8 | -4.8 | -2.2 | -4.7 | -3.5 | -3.8 | -5.1 | -0.9 | -3.4 | -1.5 | 5.0 | 2.4 | 3.6 |
SpindlePole | 0 | 0 | 1.4 | 1.3 | 2.5 | 0 | 0 | 0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.5 | -2.1 | -0.9 | -1.3 | -1.7 | -3.8 | -3.6 | -2.4 | -3.7 | -0.9 | -0.9 | 1.0 | 3.0 | 3.5 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.1972 | 8.9386 | 8.9807 | 8.5167 | 7.7368 | 8.9142 | 9.8507 | 11.7513 | 10.427 | 9.3735 | 8.6505 | 10.2607 | 6.4669 | 7.0758 | 6.984 | 6.3097 | 5.2429 | 6.7558 |
Actin | 0.0325 | 0.009 | 0.0267 | 0 | 0.0067 | 0.0024 | 0.0141 | 0.0579 | 0.0193 | 0.0003 | 0.003 | 0.0059 | 0.0117 | 0.0065 | 0.0083 | 0.0009 | 0.005 | 0.0081 |
Bud | 0.0003 | 0.0005 | 0.0003 | 0 | 0.0005 | 0 | 0.0004 | 0.0015 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Bud Neck | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0014 | 0 | 0.0003 | 0.0004 | 0.0002 | 0 | 0.0002 | 0 | 0.0003 | 0.0004 | 0.0001 | 0 | 0.0003 | 0.0003 |
Bud Periphery | 0.0008 | 0.0013 | 0.0005 | 0 | 0.0009 | 0.0001 | 0.0005 | 0.0015 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0016 | 0.0007 | 0.0004 | 0.0011 | 0.0003 |
Bud Site | 0.0007 | 0.0024 | 0.0016 | 0 | 0.0079 | 0.0001 | 0.0012 | 0.0141 | 0.001 | 0 | 0.0007 | 0.0001 | 0.0011 | 0.005 | 0.0006 | 0.0001 | 0.0003 | 0.0003 |
Cell Periphery | 0.0002 | 0.0003 | 0.0003 | 0 | 0.0006 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0001 |
Cytoplasm | 0.0008 | 0.0004 | 0.0008 | 0 | 0.0058 | 0.0003 | 0.0192 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0107 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 |
Cytoplasmic Foci | 0.007 | 0.0033 | 0.0075 | 0 | 0.0141 | 0.0101 | 0.0093 | 0.0059 | 0.0011 | 0.0001 | 0.0244 | 0.0005 | 0.0162 | 0.0097 | 0.0042 | 0.0023 | 0.004 | 0.0025 |
Eisosomes | 0.0006 | 0.0002 | 0.0014 | 0 | 0.0007 | 0.0002 | 0.0005 | 0.0006 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0007 | 0.0004 | 0.0003 | 0 | 0.003 | 0.0002 | 0.0011 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0089 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 |
Endosome | 0.0112 | 0.0142 | 0.0167 | 0 | 0.0329 | 0.0069 | 0.0272 | 0.0074 | 0.0037 | 0.0002 | 0.0292 | 0.0031 | 0.0228 | 0.0149 | 0.0153 | 0.0038 | 0.0613 | 0.0046 |
Golgi | 0.0222 | 0.0162 | 0.0142 | 0.0001 | 0.0336 | 0.0079 | 0.0507 | 0.0199 | 0.0086 | 0.0004 | 0.0393 | 0.0071 | 0.0256 | 0.0425 | 0.01 | 0.0095 | 0.0444 | 0.0148 |
Lipid Particles | 0.0158 | 0.0047 | 0.0079 | 0.0001 | 0.0375 | 0.011 | 0.074 | 0.0438 | 0.0046 | 0.0001 | 0.0702 | 0.0027 | 0.0367 | 0.0075 | 0.021 | 0.012 | 0.0259 | 0.0062 |
Mitochondria | 0.8829 | 0.9254 | 0.9079 | 0.9994 | 0.7828 | 0.9465 | 0.6638 | 0.7953 | 0.9499 | 0.9976 | 0.7173 | 0.9715 | 0.8032 | 0.8711 | 0.8966 | 0.9271 | 0.7788 | 0.9525 |
None | 0.0009 | 0.0002 | 0.0003 | 0 | 0.0333 | 0.0003 | 0.0847 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0046 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0017 | 0.0003 | 0.0002 | 0 | 0.0037 | 0.0009 | 0.0026 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0021 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 |
Nucleolus | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0037 | 0.0001 | 0.0013 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Nucleus | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0031 | 0.0001 | 0.0017 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 |
Peroxisomes | 0.0149 | 0.0101 | 0.007 | 0.0001 | 0.0106 | 0.0035 | 0.0307 | 0.0485 | 0.0031 | 0.0009 | 0.1082 | 0.0055 | 0.0408 | 0.0343 | 0.0308 | 0.0335 | 0.0576 | 0.0053 |
Punctate Nuclear | 0.0021 | 0.0001 | 0.0006 | 0 | 0.0092 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0011 | 0 | 0.0061 | 0.0004 | 0.0003 | 0 | 0.0001 | 0 |
Vacuole | 0.0013 | 0.0055 | 0.0022 | 0 | 0.0048 | 0.0005 | 0.0061 | 0.0008 | 0.0014 | 0 | 0.0015 | 0.0011 | 0.0025 | 0.0015 | 0.0058 | 0.0028 | 0.0084 | 0.0005 |
Vacuole Periphery | 0.0027 | 0.0047 | 0.0029 | 0.0003 | 0.0031 | 0.0085 | 0.0097 | 0.0014 | 0.0047 | 0.0002 | 0.0029 | 0.002 | 0.0038 | 0.0024 | 0.0044 | 0.0067 | 0.0107 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.6524 | 25.8891 | 17.2898 | 24.7177 | 18.7744 | 26.0502 | 23.886 | 23.8509 | 27.6667 | 28.0995 |
Translational Efficiency | 1.6681 | 1.0272 | 1.5981 | 1.1979 | 1.4436 | 0.9914 | 1.3752 | 1.5226 | 1.4204 | 1.2474 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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