Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.12 0.1 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.16 0.16 0.09 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0.05 0 0.06 0.05 0.08 0 0 0 0 0 0
Golgi 0 0 0.21 0.16 0.05 0 0 0 0 0 0 0 0.17 0.31 0.33 0.1 0.29 0.22 0 0.07 0 0.05 0.11 0.07
Mitochondria 0.97 0.93 0.78 0.88 0.87 0.81 0.87 0.72 0.94 0.89 0.92 0.91 0.85 0.79 0.75 0.05 0.05 0.08 0.84 0.78 0.81 0.65 0.56 0.64
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Nuclear Periphery 0.07 0 0 0 0.05 0.08 0.08 0.11 0.08 0.1 0.08 0.1 0.07 0.08 0 0 0.06 0.05 0 0 0 0 0 0
Nucleolus 0.08 0 0 0.05 0.06 0.07 0.1 0.08 0.07 0.08 0.06 0.05 0 0 0 0.06 0 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.11 0.14 0.05 0 0.07 0 0 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.13 0.12 0 0.07 0.1 0.09 0.09 0.05 0.06 0.07 0.06 0.09 0.09 0.15 0.23 0.24 0.26 0 0.06 0 0.06 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 6 6 3 3 0 1 1 4 1 1 1 2 0 3 0 3 4 4 6 3 4 5
Bud 0 9 3 6 5 4 12 6 10 12 16 22 2 1 0 9 2 6 0 1 2 5 9 5
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 1 2 2
Bud Site 0 0 0 0 1 5 3 3 3 1 0 4 0 0 0 0 1 0
Cell Periphery 1 10 18 3 2 8 1 4 3 6 4 2 4 1 2 20 17 19 0 0 0 0 0 0
Cytoplasm 0 7 7 6 14 9 20 18 5 5 3 8 3 7 6 21 22 18 0 1 0 9 11 6
Endoplasmic Reticulum 0 9 1 2 3 2 1 2 9 5 2 6 0 4 3 3 6 4 0 0 2 0 2 1
Endosome 0 2 15 5 3 26 3 52 3 14 11 5 4 10 5 8 7 15 4 6 10 8 7 8
Golgi 5 1 80 45 15 20 1 13 0 10 2 7 25 66 64 13 40 42 4 21 19 10 30 18
Mitochondria 147 275 293 252 268 429 475 444 485 619 495 604 122 167 145 6 7 15 127 234 361 133 159 163
Nucleus 0 1 3 0 5 2 15 6 3 3 2 8 3 2 2 3 1 5 0 1 0 6 13 6
Nuclear Periphery 11 7 14 9 14 42 44 67 39 67 42 70 10 17 8 4 8 9 1 2 2 1 3 3
Nucleolus 12 6 7 13 18 38 54 48 35 56 32 36 4 1 3 8 3 16 0 0 1 1 3 4
Peroxisomes 3 1 20 11 4 10 2 16 2 11 6 3 5 0 5 26 16 27 7 6 32 5 11 14
SpindlePole 0 0 3 1 2 10 10 20 4 9 8 11 2 0 0 1 0 11 0 0 1 2 3 5
Vac/Vac Membrane 14 39 45 12 22 53 51 53 26 40 39 37 13 20 29 30 34 50 2 16 4 12 19 4
Unique Cell Count 152 296 377 288 309 530 545 616 515 697 540 667 144 211 194 132 140 192 153 301 446 206 286 257
Labelled Cell Count 193 367 515 371 379 661 692 754 628 862 663 825 198 298 272 155 164 240 153 301 446 206 286 257


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.9 7.4 10.3 9.9 7.3 8.2 5.3 8.1 7.0 7.6 7.4 6.9 9.0 9.2 9.0 9.0 11.1 11.3 10.2 10.2 10.6
Std Deviation (1e-4) 2.0 1.6 1.8 1.8 1.7 1.6 1.4 1.6 1.5 1.6 1.7 1.6 1.8 1.4 1.6 2.4 2.8 3.2 2.6 2.9 2.9
Intensity Change (Log2) -0.05 -0.5 -0.33 -0.95 -0.34 -0.55 -0.45 -0.48 -0.59 -0.2 -0.17 -0.2 -0.19 0.11 0.13 -0.01 -0.01 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1.7 0 1.4 1.2 2.3 2.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.7 -3.2 -2.9 -4.8 -4.3 -4.1 -4.1 -3.9 -5.1 -1.0 -2.8 -2.3 3.9 3.0 2.3
Cytoplasm 0.2 2.0 -0.2 1.6 1.0 -1.1 0 0 -0.9 0 1.1 0 6.1 6.1 4.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.7 -2.5 0.7 -3.7 2.7 -3.6 -1.9 -1.7 -3.7 -0.7 0.4 -0.9 1.0 0.5 1.9
Golgi -1.8 -6.2 -8.3 -11.1 -10.0 -11.0 -11.2 -10.9 -11.3 -1.0 2.7 3.1 -2.9 1.8 0.2
Mitochondria 3.2 3.0 1.2 3.8 -2.0 7.3 4.8 6.0 5.7 1.8 0.4 -0.8 -14.7 -14.9 -15.8
Nucleus 0 0 0 2.1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.4 0.5 2.6 2.7 4.0 2.4 3.5 2.5 3.9 1.6 2.3 0.2 0 1.0 0.6
Nucleolus 2.0 2.8 3.6 4.8 4.0 3.4 4.1 3.0 2.8 0 0 0 0 0 3.7
Peroxisomes -0.9 -2.8 -2.8 -4.8 -2.2 -4.7 -3.5 -3.8 -5.1 -0.9 -3.4 -1.5 5.0 2.4 3.6
SpindlePole 0 0 1.4 1.3 2.5 0 0 0 1.2 0 0 0 0 0 0
Vacuole -3.5 -2.1 -0.9 -1.3 -1.7 -3.8 -3.6 -2.4 -3.7 -0.9 -0.9 1.0 3.0 3.5 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1972 8.9386 8.9807 8.5167 7.7368 8.9142 9.8507 11.7513 10.427 9.3735 8.6505 10.2607 6.4669 7.0758 6.984 6.3097 5.2429 6.7558
Actin 0.0325 0.009 0.0267 0 0.0067 0.0024 0.0141 0.0579 0.0193 0.0003 0.003 0.0059 0.0117 0.0065 0.0083 0.0009 0.005 0.0081
Bud 0.0003 0.0005 0.0003 0 0.0005 0 0.0004 0.0015 0.0003 0 0.0002 0.0001 0.0003 0.0006 0.0003 0.0001 0.0003 0.0001
Bud Neck 0.0003 0.0002 0.0003 0 0.0014 0 0.0003 0.0004 0.0002 0 0.0002 0 0.0003 0.0004 0.0001 0 0.0003 0.0003
Bud Periphery 0.0008 0.0013 0.0005 0 0.0009 0.0001 0.0005 0.0015 0.0005 0.0001 0.0004 0.0001 0.0005 0.0016 0.0007 0.0004 0.0011 0.0003
Bud Site 0.0007 0.0024 0.0016 0 0.0079 0.0001 0.0012 0.0141 0.001 0 0.0007 0.0001 0.0011 0.005 0.0006 0.0001 0.0003 0.0003
Cell Periphery 0.0002 0.0003 0.0003 0 0.0006 0.0001 0.0003 0.0002 0.0002 0 0.0001 0 0.0002 0.0003 0.0002 0.0003 0.0003 0.0001
Cytoplasm 0.0008 0.0004 0.0008 0 0.0058 0.0003 0.0192 0.0002 0.0002 0 0.0001 0 0.0107 0.0002 0.0001 0 0.0002 0
Cytoplasmic Foci 0.007 0.0033 0.0075 0 0.0141 0.0101 0.0093 0.0059 0.0011 0.0001 0.0244 0.0005 0.0162 0.0097 0.0042 0.0023 0.004 0.0025
Eisosomes 0.0006 0.0002 0.0014 0 0.0007 0.0002 0.0005 0.0006 0.0002 0 0.0001 0.0002 0.0003 0.0003 0.0003 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.0007 0.0004 0.0003 0 0.003 0.0002 0.0011 0.0001 0.0003 0 0.0001 0 0.0089 0.0001 0.0001 0 0.0003 0
Endosome 0.0112 0.0142 0.0167 0 0.0329 0.0069 0.0272 0.0074 0.0037 0.0002 0.0292 0.0031 0.0228 0.0149 0.0153 0.0038 0.0613 0.0046
Golgi 0.0222 0.0162 0.0142 0.0001 0.0336 0.0079 0.0507 0.0199 0.0086 0.0004 0.0393 0.0071 0.0256 0.0425 0.01 0.0095 0.0444 0.0148
Lipid Particles 0.0158 0.0047 0.0079 0.0001 0.0375 0.011 0.074 0.0438 0.0046 0.0001 0.0702 0.0027 0.0367 0.0075 0.021 0.012 0.0259 0.0062
Mitochondria 0.8829 0.9254 0.9079 0.9994 0.7828 0.9465 0.6638 0.7953 0.9499 0.9976 0.7173 0.9715 0.8032 0.8711 0.8966 0.9271 0.7788 0.9525
None 0.0009 0.0002 0.0003 0 0.0333 0.0003 0.0847 0.0001 0.0002 0 0.0002 0 0.0046 0.0001 0.0001 0 0.0001 0
Nuclear Periphery 0.0017 0.0003 0.0002 0 0.0037 0.0009 0.0026 0 0.0003 0 0.0003 0 0.0021 0.0001 0.0003 0 0.0003 0.0001
Nucleolus 0.0002 0.0002 0.0001 0 0.0037 0.0001 0.0013 0.0002 0.0001 0 0.0003 0 0.0005 0.0005 0.0003 0.0002 0.0002 0.0001
Nucleus 0.0002 0.0003 0.0001 0 0.0031 0.0001 0.0017 0.0001 0.0001 0 0.0002 0 0.001 0.0001 0.0001 0.0001 0.0002 0
Peroxisomes 0.0149 0.0101 0.007 0.0001 0.0106 0.0035 0.0307 0.0485 0.0031 0.0009 0.1082 0.0055 0.0408 0.0343 0.0308 0.0335 0.0576 0.0053
Punctate Nuclear 0.0021 0.0001 0.0006 0 0.0092 0.0003 0.0007 0.0001 0.0001 0 0.0011 0 0.0061 0.0004 0.0003 0 0.0001 0
Vacuole 0.0013 0.0055 0.0022 0 0.0048 0.0005 0.0061 0.0008 0.0014 0 0.0015 0.0011 0.0025 0.0015 0.0058 0.0028 0.0084 0.0005
Vacuole Periphery 0.0027 0.0047 0.0029 0.0003 0.0031 0.0085 0.0097 0.0014 0.0047 0.0002 0.0029 0.002 0.0038 0.0024 0.0044 0.0067 0.0107 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.6524 25.8891 17.2898 24.7177 18.7744 26.0502 23.886 23.8509 27.6667 28.0995
Translational Efficiency 1.6681 1.0272 1.5981 1.1979 1.4436 0.9914 1.3752 1.5226 1.4204 1.2474

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl15

Mrpl15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available