Standard name
Description Component of the exomer complex; the exomer which also contains Csh6p, Bch1p, Bch2p, and Bud7, is involved in the export of select proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane; interacts selectively with the activated, GTP-bound form of Arf1p; Chs5p is the only protein with a BRCT domain that is not localized to the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.09 0.11 0.06 0 0 0.08 0 0 0 0 0.11 0.06 0 0.1 0.15 0.1 0.11 0.17 0.12 0.09 0.11 0.14
Bud 0 0 0 0 0 0 0 0.06 0 0.05 0.07 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.1 0.07 0 0.08 0.05 0.05 0 0 0.07 0.05 0.05 0.14 0.14 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0 0 0 0 0.08 0 0.05 0 0.06 0 0 0 0 0 0.05 0 0.05 0.08 0.06 0 0
Golgi 0.11 0.39 0.68 0.37 0.4 0.06 0.08 0.12 0 0 0 0 0.64 0.47 0.31 0.65 0.68 0.67 0.61 0.59 0.56 0.62 0.72 0.68
Mitochondria 0.91 0.81 0.44 0.73 0.56 0.78 0.69 0.66 0.77 0.69 0.76 0.67 0.62 0.66 0.52 0.07 0.07 0.08 0 0.06 0 0.1 0.05 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0.07 0.07 0 0.06 0.08 0.11 0.09 0.13 0.11 0.13 0.12 0 0 0.05 0.11 0.05 0.08 0 0 0 0 0 0
Peroxisomes 0.06 0 0 0.1 0.12 0.08 0.21 0.15 0.13 0.17 0.18 0.24 0 0.06 0.22 0 0 0 0.18 0.08 0.15 0.05 0 0
SpindlePole 0 0 0 0 0 0 0.05 0.05 0 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.09 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0.07 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 9 11 13 9 3 9 15 2 3 5 5 22 18 3 10 14 4 35 29 18 12 29 34
Bud 6 2 2 1 2 4 9 11 4 12 17 17 4 12 5 2 4 2 2 1 0 2 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 3 1 1 3 3 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 5 5 4 8 16 11 10 15 9 12 8 8 15 13 12 14 13 3 0 2 1 3 0 4
Endoplasmic Reticulum 2 1 0 0 0 0 0 0 0 1 0 0 4 3 0 2 2 1 0 0 0 0 0 0
Endosome 2 0 6 2 3 4 5 15 4 14 5 15 6 4 7 2 0 2 9 8 12 8 9 7
Golgi 33 65 79 42 63 9 23 24 2 6 2 8 129 135 77 63 62 26 189 101 84 81 196 173
Mitochondria 271 133 51 83 88 114 192 130 133 176 178 169 125 189 129 7 6 3 9 9 5 13 13 17
Nucleus 1 1 0 0 0 1 1 0 0 1 4 0 1 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 1 2 3 3 1 0 4 2 2 0 2 1 0 0 0 0 0 0 0 1 0
Nucleolus 15 12 8 4 9 12 31 17 23 27 30 29 7 8 13 11 5 3 2 0 1 0 0 0
Peroxisomes 18 3 2 11 19 12 58 29 23 43 42 61 2 18 56 2 3 0 54 14 22 6 10 7
SpindlePole 0 0 1 1 2 4 14 10 6 15 11 17 0 1 5 1 2 0 3 2 0 0 0 0
Vac/Vac Membrane 4 3 10 0 4 1 6 5 2 6 2 2 10 6 9 6 6 4 2 2 1 1 4 1
Unique Cell Count 299 165 116 114 157 147 280 198 172 256 235 251 201 287 250 97 91 39 310 174 151 131 273 254
Labelled Cell Count 365 235 174 166 217 179 364 273 209 324 309 338 326 409 319 120 117 48 310 174 151 131 273 254


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.0 21.1 17.5 16.1 16.5 14.2 14.0 14.1 11.6 11.9 10.8 11.4 18.8 19.5 19.0 20.4 22.1 21.9 16.7 17.6 17.6
Std Deviation (1e-4) 4.0 4.3 3.4 3.3 3.6 3.0 3.3 3.7 2.9 3.1 3.0 2.9 4.7 6.3 6.2 6.1 6.0 6.6 4.0 3.5 3.6
Intensity Change (Log2) – – – -0.12 -0.09 -0.3 -0.32 -0.32 -0.6 -0.56 -0.69 -0.62 0.1 0.15 0.11 0.22 0.33 0.32 -0.07 0.01 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 -1.2 -2.7 -2.6 -0.6 -3.3 0 -3.1 -3.3 0.4 -1.1 0 0.2 1.3 0
Bud 0 0 0 0 0 0 0 2.1 2.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 2.1 1.4 0 1.5 0.7 0 0 0 1.5 0 0.6 2.9 2.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.8 0 0.1 0 0.3 0 0 0 0 0 0
Golgi -4.7 -4.6 -10.6 -12.4 -10.2 -12.4 -14.0 -14.1 -13.6 -0.7 -3.8 -6.7 -0.5 0 -0.2
Mitochondria 4.4 2.0 5.6 4.6 3.8 5.8 4.5 5.9 4.2 3.1 4.1 1.4 -6.0 -6.0 -4.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.2 -0.4 0.4 1.3 0.5 1.7 1.1 1.7 1.4 -1.4 0 -0.6 1.1 -0.4 0
Peroxisomes 2.6 3.2 2.3 4.8 3.7 3.4 4.1 4.3 5.3 0 1.9 5.0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 2.4 0 0 0 0 0 0
Vacuole 0 -2.2 0 0 -2.4 0 0 0 0 -1.3 0 -2.0 -0.7 -0.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.1803 13.4778 12.9879 11.9058 11.5727 12.5777 12.0488 13.6938 12.4623 12.7865 11.0364 13.17 16.0695 15.5209 15.3816 14.3427 14.9338 15.2884
Actin 0.0512 0.0609 0.0876 0.0121 0.0341 0.1194 0.0579 0.0338 0.048 0.0082 0.0158 0.0581 0.0564 0.0344 0.0442 0.1044 0.0609 0.0522
Bud 0.0005 0.0003 0.0007 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0003 0 0
Bud Neck 0.0004 0.0004 0.0003 0.0005 0.0003 0.0016 0.0002 0.0001 0.0002 0.0002 0.0001 0.0003 0.0003 0.0001 0.0002 0.0013 0.0001 0.0002
Bud Periphery 0.001 0.0003 0.0005 0.0005 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0003 0 0
Bud Site 0.0015 0.001 0.0005 0.001 0.0006 0.0002 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0018 0.0001 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0.0018 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0
Cytoplasm 0.0035 0.0004 0.0017 0.0157 0.0002 0.0002 0.0005 0.0002 0.0004 0.0003 0.0003 0.0001 0.0012 0.0003 0.0002 0.0015 0.0002 0.0003
Cytoplasmic Foci 0.068 0.068 0.0479 0.044 0.0307 0.0328 0.0701 0.0582 0.0507 0.0534 0.0717 0.0353 0.0569 0.0533 0.0403 0.0578 0.0438 0.0282
Eisosomes 0.0002 0.0001 0.0002 0.0006 0.0001 0.0003 0.0002 0.0001 0.0002 0 0.0001 0.0002 0.0003 0 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0016 0.0001 0.0002 0.011 0.0002 0.0001 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0.0014 0.0001 0.0001
Endosome 0.0592 0.0868 0.0688 0.0989 0.0522 0.0404 0.0515 0.0515 0.061 0.108 0.0468 0.0344 0.0493 0.0644 0.0471 0.0554 0.0561 0.0385
Golgi 0.5309 0.6634 0.644 0.6997 0.7308 0.6842 0.4807 0.6444 0.6357 0.6662 0.7131 0.6001 0.6648 0.7677 0.7684 0.6928 0.7579 0.7843
Lipid Particles 0.1244 0.0373 0.0639 0.0391 0.0432 0.0455 0.1384 0.054 0.0652 0.0786 0.0481 0.0994 0.064 0.0398 0.0386 0.0396 0.0326 0.0289
Mitochondria 0.0655 0.0283 0.0544 0.034 0.0894 0.0271 0.0728 0.055 0.0531 0.0113 0.0625 0.0652 0.0427 0.0172 0.0308 0.0045 0.0222 0.0272
None 0.0052 0 0.0003 0.0199 0.0001 0 0.0004 0 0.0002 0 0.0001 0.0001 0.0013 0 0 0.0022 0 0.0001
Nuclear Periphery 0.0014 0.0001 0.0001 0.002 0.0001 0 0.0004 0 0.001 0.0001 0.0001 0.0003 0.0038 0 0 0.002 0 0.0001
Nucleolus 0.0005 0 0.0001 0.0003 0.0001 0 0.0003 0 0.0011 0 0.0001 0.0001 0.0002 0 0 0.0007 0 0
Nucleus 0.0008 0 0.0001 0.0012 0.0001 0 0.0001 0 0.0002 0 0 0 0.0006 0 0 0.0013 0 0.0001
Peroxisomes 0.0773 0.05 0.0251 0.0111 0.0142 0.0465 0.1165 0.1009 0.0761 0.0713 0.0374 0.1033 0.0509 0.0184 0.0272 0.0268 0.0248 0.0362
Punctate Nuclear 0.0025 0.0001 0.0005 0.0004 0.0002 0.0001 0.006 0.0001 0.0021 0.0001 0.0006 0.0001 0.0043 0 0 0.0035 0 0.002
Vacuole 0.002 0.0007 0.0011 0.0024 0.0007 0.0003 0.001 0.0002 0.0011 0.0007 0.0005 0.0004 0.0004 0.0007 0.0005 0.0009 0.0002 0.0002
Vacuole Periphery 0.0021 0.0016 0.0017 0.0035 0.0017 0.0009 0.0019 0.001 0.0026 0.0012 0.0022 0.0022 0.0013 0.0031 0.0019 0.001 0.0007 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.4793 64.5842 70.071 66.4071 74.1562 73.8478 76.0608 76.1274 91.3274 68.9633
Translational Efficiency 1.5395 1.4022 1.1035 1.3949 1.1558 1.0915 0.9819 1.1613 0.9701 1.1433

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘