Standard name
Description Nucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.08 0.11 0.08 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.05 0 0 0 0.13 0.18 – 0.3 0.33 0.39 0.2 0.06 0.08 0.07 0.1 0 0.08 0 0 0 0.08 0.13 0.14
Nucleus 0.1 0.07 0 0.06 0.05 0 0.07 – 0.07 0.08 0.07 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.73 0.67 0.84 0.84 0.76 0.71 0.62 – 0.55 0.53 0.42 0.54 0.89 0.79 0.74 0.6 0.6 0.59 0.83 0.79 0.91 0.8 0.73 0.7
Nucleolus 0.23 0.18 0.08 0.13 0.13 0.23 0.31 – 0.33 0.38 0.4 0.38 0.26 0.24 0.28 0.17 0.21 0.19 0.06 0.06 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.27 0.24 0.1 0.13 0.12 0.08 – 0.08 0.07 0.07 0.07 0.08 0.11 0.11 0.32 0.4 0.32 0.05 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 1 – 0 1 0 0 0 1 1 0 0 0 0 0 0 3 3 4
Bud 0 0 0 2 0 1 4 – 0 2 4 2 0 0 0 0 0 0 0 3 2 0 2 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 0 0 1 – 2 2 1 6 1 3 4 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 8 1 8 10 8 4 – 3 5 4 6 9 27 40 32 21 21 0 2 0 0 0 0
Endoplasmic Reticulum 0 1 3 4 4 0 3 – 1 0 0 0 0 2 4 6 9 7 0 0 1 2 2 0
Endosome 0 0 3 1 3 10 14 – 5 2 2 21 1 3 13 3 5 3 0 1 1 4 12 18
Golgi 0 0 0 1 1 0 0 – 0 0 0 1 0 0 1 2 5 2 0 0 0 2 5 7
Mitochondria 11 14 4 5 13 51 74 – 113 120 154 83 29 32 38 28 11 17 2 5 11 25 57 58
Nucleus 17 19 15 19 17 18 30 – 25 29 26 29 12 6 6 4 2 10 6 4 4 0 1 1
Nuclear Periphery 126 194 310 251 255 290 262 – 206 194 168 220 433 334 393 171 148 124 155 271 380 257 319 290
Nucleolus 39 52 29 38 45 93 131 – 121 137 159 154 125 102 151 47 51 40 10 21 4 7 5 5
Peroxisomes 0 0 0 0 0 1 0 – 1 0 0 4 0 3 0 0 0 0 0 0 0 5 12 9
SpindlePole 0 0 0 1 1 3 2 – 1 2 3 1 2 7 15 1 2 2 1 1 1 0 1 2
Vac/Vac Membrane 33 77 87 30 44 48 32 – 30 25 27 29 41 47 59 91 99 68 8 28 10 11 9 10
Unique Cell Count 172 288 367 299 334 406 421 372 365 398 406 485 421 532 284 248 210 188 343 419 322 440 415
Labelled Cell Count 227 365 453 361 394 524 558 508 519 548 556 653 567 725 385 353 294 188 343 419 322 440 415


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.7 18.2 15.0 13.6 13.8 11.1 11.0 – 9.3 8.4 8.1 9.3 15.1 13.9 13.8 21.9 23.5 20.4 13.8 13.4 13.8
Std Deviation (1e-4) 3.8 5.3 3.6 3.7 3.8 2.7 2.8 – 2.5 2.1 2.1 2.8 3.9 4.3 4.6 7.8 8.0 7.8 3.6 3.1 2.8
Intensity Change (Log2) – – – -0.14 -0.12 -0.43 -0.45 – -0.69 -0.84 -0.9 -0.69 0.01 -0.11 -0.12 0.55 0.64 0.44 -0.12 -0.16 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.9 0 0 – 0 0 0 0 0 4.6 5.1 6.3 5.4 5.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 1.8 2.4 – 0 0 0 3.5 0 0 1.8 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.4 6.2 7.7 – 10.9 11.5 12.8 8.5 3.7 4.4 4.2 5.1 2.6 4.3
Nucleus 1.3 0.6 0.2 1.8 – 1.6 2.2 1.5 1.8 -1.3 -2.3 -2.9 -2.0 -2.4 0.4
Nuclear Periphery -0.2 -2.7 -4.3 -7.0 – -8.6 -9.1 -12.1 -9.1 2.1 -1.9 -3.8 -7.0 -6.9 -6.8
Nucleolus 2.1 2.4 5.7 8.1 – 8.3 9.6 10.3 9.8 6.7 6.1 7.5 3.4 4.6 4.0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 3.2 0 0 0
Vacuole -4.6 -3.6 -4.3 -6.3 – -5.8 -6.3 -6.6 -6.4 -6.2 -4.7 -5.0 2.4 4.3 2.3