Standard name
Description Catalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.26 0.11 0.2 0.11 0.22 0.27 0.36 0.42 0.43 0.53 0.46 0.66 0.83 0.15 0.27 0.25 0 0.06 0.05 0.08 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.08 0.05 0.05 0.05 0 0.13 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0.33 0.24 0.1 0.18 0.19 0.24 0.18 0.23 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.06 0.06 0.11 0.16 0.14 0.2 0.19 0.14 0.17 0.13 0.09 0 0 0 0.23 0.15 0.25 0 0 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.77 0.75 0.64 0.63 0.79 0.66 0.63 0.61 0.59 0.57 0.49 0.51 0.38 0.21 0.65 0.58 0.59 0.81 0.77 0.8 0.64 0.78 0.82
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 2 0 1 0 9 4 4 1 3 2 3 2 0 0 0 8 4 1 2 11 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 21 21 7 64 56 88 248 189 256 137 296 175 420 618 25 48 48 4 5 4 7 14 10
Endoplasmic Reticulum 3 0 1 0 8 4 9 3 1 1 4 8 11 2 7 4 8 2 0 0 1 3 2
Endosome 15 3 0 6 13 1 4 0 2 0 0 12 30 7 9 15 9 9 4 2 11 16 33
Golgi 0 0 0 0 3 0 0 0 0 0 0 0 1 0 0 3 5 0 0 0 0 1 1
Mitochondria 19 0 20 76 52 74 174 123 112 73 126 14 2 0 6 7 4 3 1 2 3 4 5
Nucleus 11 5 7 50 75 80 179 75 102 41 50 11 18 2 39 27 48 2 1 4 2 5 16
Nuclear Periphery 2 1 0 2 4 0 3 3 2 0 1 2 0 0 0 0 1 1 1 0 0 3 6
Nucleolus 0 0 0 3 0 0 1 0 2 1 0 1 0 0 1 2 2 0 2 0 0 1 3
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 2 1 1 0 0 1 0 0 0 3 2 3 0 1 1 4 4
Vac/Vac Membrane 140 60 39 203 413 265 584 319 357 184 270 195 246 159 108 105 116 159 74 67 56 258 431
Unique Cell Count 182 80 61 322 525 401 931 522 609 321 556 381 639 747 166 180 195 198 97 85 88 332 528
Labelled Cell Count 211 90 76 405 626 514 1215 717 839 438 750 421 731 790 195 215 243 198 97 85 88 332 528


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.4 5.3 6.0 8.2 7.1 6.6 5.9 5.8 5.5 5.3 6.4 6.1 5.4 8.9 9.5 9.2 7.3 7.5 7.6
Std Deviation (1e-4) 2.4 2.1 1.6 2.0 2.5 3.0 2.8 2.8 2.6 2.5 2.5 2.0 1.4 1.2 2.6 3.2 2.7 2.4 2.5 2.4
Intensity Change (Log2) 0.16 0.62 0.42 0.3 0.15 0.12 0.05 0.0 0.26 0.19 0.01 0.74 0.83 0.78 0.46 0.5 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 -0.2 1.9 2.6 3.9 4.7 4.6 6.2 5.1 8.3 12.8 0.7 2.4 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.5 -5.2 -2.6 -2.7 -1.6 -2.7 -1.7 -1.8 0 0 0 -6.1 -6.2 -7.2
Nucleus 0.8 0.6 1.6 1.5 0.6 1.1 0.3 -0.6 0 0 0 2.0 0.7 2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.1 2.6 0.3 -0.2 -0.4 -0.8 -1.0 -2.3 -1.9 -3.9 -7.4 0.2 -0.8 -0.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fks1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available