Standard name
Human Ortholog
Description Catalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.26 0.11 0.2 0.11 0.22 0.27 0.36 0.42 0.43 0.53 0.46 0.66 0.83 0.15 0.27 0.25 0 0.06 0.05 0.08 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.08 0.05 0.05 0.05 0 0.13 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0.33 0.24 0.1 0.18 0.19 0.24 0.18 0.23 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.06 0.06 0.11 0.16 0.14 0.2 0.19 0.14 0.17 0.13 0.09 0 0 0 0.23 0.15 0.25 0 0 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.77 0.75 0.64 0.63 0.79 0.66 0.63 0.61 0.59 0.57 0.49 0.51 0.38 0.21 0.65 0.58 0.59 0.81 0.77 0.8 0.64 0.78 0.82
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 2 0 1 0 9 4 4 1 3 2 3 2 0 0 0 8 4 1 2 11 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 21 21 7 64 56 88 248 189 256 137 296 175 420 618 25 48 48 4 5 4 7 14 10
Endoplasmic Reticulum 3 0 1 0 8 4 9 3 1 1 4 8 11 2 7 4 8 2 0 0 1 3 2
Endosome 15 3 0 6 13 1 4 0 2 0 0 12 30 7 9 15 9 9 4 2 11 16 33
Golgi 0 0 0 0 3 0 0 0 0 0 0 0 1 0 0 3 5 0 0 0 0 1 1
Mitochondria 19 0 20 76 52 74 174 123 112 73 126 14 2 0 6 7 4 3 1 2 3 4 5
Nucleus 11 5 7 50 75 80 179 75 102 41 50 11 18 2 39 27 48 2 1 4 2 5 16
Nuclear Periphery 2 1 0 2 4 0 3 3 2 0 1 2 0 0 0 0 1 1 1 0 0 3 6
Nucleolus 0 0 0 3 0 0 1 0 2 1 0 1 0 0 1 2 2 0 2 0 0 1 3
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 2 1 1 0 0 1 0 0 0 3 2 3 0 1 1 4 4
Vac/Vac Membrane 140 60 39 203 413 265 584 319 357 184 270 195 246 159 108 105 116 159 74 67 56 258 431
Unique Cell Count 182 80 61 322 525 401 931 522 609 321 556 381 639 747 166 180 195 198 97 85 88 332 528
Labelled Cell Count 211 90 76 405 626 514 1215 717 839 438 750 421 731 790 195 215 243 198 97 85 88 332 528


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.4 5.3 6.0 8.2 7.1 6.6 5.9 5.8 5.5 5.3 6.4 6.1 5.4 8.9 9.5 9.2 7.3 7.5 7.6
Std Deviation (1e-4) 2.4 2.1 1.6 2.0 2.5 3.0 2.8 2.8 2.6 2.5 2.5 2.0 1.4 1.2 2.6 3.2 2.7 2.4 2.5 2.4
Intensity Change (Log2) 0.16 0.62 0.42 0.3 0.15 0.12 0.05 0.0 0.26 0.19 0.01 0.74 0.83 0.78 0.46 0.5 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 -0.2 1.9 2.6 3.9 4.7 4.6 6.2 5.1 8.3 12.8 0.7 2.4 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.5 -5.2 -2.6 -2.7 -1.6 -2.7 -1.7 -1.8 0 0 0 -6.1 -6.2 -7.2
Nucleus 0.8 0.6 1.6 1.5 0.6 1.1 0.3 -0.6 0 0 0 2.0 0.7 2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.1 2.6 0.3 -0.2 -0.4 -0.8 -1.0 -2.3 -1.9 -3.9 -7.4 0.2 -0.8 -0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5138 8.3122 6.6171 5.9039 5.4512 6.5516 2.2787 8.9059 7.5526 5.8052 4.8155 6.204 4.2021 9.0912 9.8737 8.3177 8.3353 9.0068
Actin 0.0067 0 0.0021 0.0002 0.0097 0.0028 0.0063 0.0001 0.0117 0.0315 0.0048 0 0.0108 0.0102 0.0001 0 0 0
Bud 0.0029 0.0004 0.0004 0.0002 0.0004 0.0003 0.0004 0.0007 0.0003 0.0008 0.0002 0.0002 0.0008 0.0005 0.0004 0.0004 0.0004 0.0003
Bud Neck 0.001 0.0012 0.0013 0.0007 0.0013 0.003 0.001 0.0008 0.0006 0.0023 0.0012 0.0015 0.0012 0.0019 0.001 0.0037 0.0011 0.0014
Bud Periphery 0.0018 0.0011 0.0011 0.0004 0.001 0.001 0.0008 0.0016 0.0006 0.0013 0.0004 0.0007 0.0016 0.0011 0.0009 0.0011 0.0003 0.0008
Bud Site 0.002 0.0019 0.0013 0.001 0.001 0.0005 0.0017 0.0008 0.0011 0.0084 0.0009 0.0007 0.0061 0.0033 0.0015 0.002 0.0029 0.0005
Cell Periphery 0.0017 0.0041 0.0031 0.0018 0.0018 0.004 0.0024 0.005 0.0025 0.0036 0.0011 0.0037 0.0018 0.0041 0.0025 0.0043 0.0014 0.0035
Cytoplasm 0.0083 0.0029 0.0044 0.0009 0.0012 0.001 0.0026 0.0039 0.0144 0.0022 0.008 0.0057 0.0046 0.007 0.0078 0.0011 0.0033 0.0032
Cytoplasmic Foci 0.0188 0.013 0.0069 0.0038 0.0105 0.0027 0.0103 0.0029 0.0139 0.0202 0.0328 0.0057 0.0189 0.0091 0.0077 0.0047 0.0114 0.0036
Eisosomes 0.0001 0 0 0 0.0003 0.0001 0 0 0.0001 0.0004 0.0003 0 0.0002 0.0003 0 0 0 0
Endoplasmic Reticulum 0.0052 0.0053 0.0092 0.002 0.0037 0.0032 0.0034 0.0076 0.0096 0.0043 0.0035 0.0062 0.0057 0.0037 0.0099 0.0018 0.0007 0.0067
Endosome 0.2734 0.1569 0.2036 0.2123 0.2889 0.125 0.3396 0.1438 0.3381 0.2679 0.3812 0.2101 0.3197 0.1313 0.2923 0.1084 0.2966 0.192
Golgi 0.0172 0.0052 0.0096 0.012 0.0147 0.0083 0.0185 0.0068 0.0209 0.0145 0.0315 0.0108 0.0235 0.006 0.0157 0.0039 0.0079 0.0099
Lipid Particles 0.0309 0.0137 0.0108 0.0176 0.0519 0.0204 0.0334 0.0152 0.0205 0.0102 0.0523 0.0254 0.0378 0.0216 0.021 0.0244 0.0113 0.0498
Mitochondria 0.0485 0.0095 0.0159 0.0054 0.0486 0.0103 0.0176 0.0038 0.0065 0.0056 0.0082 0.0049 0.0352 0.028 0.0062 0.0026 0.0005 0.0066
None 0.0019 0.0001 0.0003 0.0001 0.0012 0.0001 0.0004 0.0001 0.0012 0.0011 0.0266 0.0002 0.001 0.0004 0.0002 0.0002 0.0001 0.0002
Nuclear Periphery 0.0135 0.006 0.0178 0.0035 0.0301 0.0155 0.0055 0.0125 0.01 0.0037 0.0322 0.0074 0.0217 0.0182 0.0071 0.0033 0.0011 0.0343
Nucleolus 0.0048 0.005 0.0032 0.0015 0.0058 0.005 0.0088 0.0026 0.0013 0.0074 0.0021 0.0025 0.0029 0.0057 0.0021 0.081 0.0031 0.0055
Nucleus 0.007 0.004 0.0097 0.0012 0.0034 0.0062 0.0046 0.0025 0.0016 0.0032 0.0041 0.0028 0.0038 0.0028 0.0027 0.0124 0.0017 0.0231
Peroxisomes 0.003 0.0009 0.0008 0.0005 0.0047 0.0005 0.0016 0.0002 0.003 0.0018 0.0052 0.0003 0.0059 0.0027 0.0004 0.0016 0.0007 0.0006
Punctate Nuclear 0.0015 0.0001 0.0003 0.0002 0.0029 0.0002 0.0005 0.0001 0.0009 0.0015 0.0218 0.0001 0.0009 0.0002 0.0001 0.0005 0.0001 0.0002
Vacuole 0.3693 0.5788 0.4938 0.5591 0.3849 0.5453 0.4018 0.5768 0.3951 0.4745 0.3111 0.5313 0.3336 0.5661 0.4728 0.5954 0.5523 0.4466
Vacuole Periphery 0.1803 0.1896 0.2041 0.1755 0.132 0.2446 0.1388 0.2125 0.1459 0.1336 0.0706 0.1796 0.1625 0.1759 0.1476 0.147 0.103 0.2112

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 483.2171 459.1177 288.3021 288.8552 440.4131 360.7434 402.9323 292.6923 236.4283 460.8302
Translational Efficiency 0.8251 0.7702 0.6771 0.5813 0.6054 0.8943 0.6781 0.5038 0.4765 0.527

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
64 620 482 1066 355 610 1840 1285 419 1230 2322 2351

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 769.59 923.75 1009.64 1160.11 795.11 949.06 1102.83 1080.90 791.21 936.30 1083.49 1116.82
Standard Deviation 135.59 196.29 118.95 205.01 201.33 159.30 185.47 184.35 192.96 179.35 177.83 197.95
Intensity Change Log 2 0.263412 0.391679 0.592100 0.255345 0.471984 0.443007 0.259318 0.433045 0.518263

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000749 0.000148 0.001259 0.009511 0.000075 0.000041 0.004514 0.007103 0.000178 0.000095 0.003838 0.008195
Bud Neck 0.010187 0.009481 0.033459 0.024949 0.030680 0.012353 0.021923 0.017908 0.027550 0.010905 0.024318 0.021100
Bud Site 0.014478 0.027733 0.031799 0.078241 0.024125 0.011134 0.052098 0.069119 0.022651 0.019501 0.047884 0.073255
Cell Periphery 0.000409 0.000910 0.000277 0.012761 0.001402 0.001169 0.005096 0.004689 0.001250 0.001038 0.004095 0.008349
Cytoplasm 0.320740* 0.039106 0.111107* 0.141773* 0.044813 0.015959 0.096648 0.166758* 0.086959 0.027627 0.099650 0.155429*
Cytoplasmic Foci 0.145919 0.035139 0.063337 0.033355 0.030888 0.015223 0.043203 0.067531 0.048458 0.025262 0.047383 0.052035
Eisosomes 0.000278 0.000022 0.000071 0.000821 0.000069 0.000034 0.000270 0.000386 0.000101 0.000028 0.000228 0.000583
Endoplasmic Reticulum 0.002838 0.003401 0.012219 0.099317 0.005468 0.006475 0.122702* 0.072259 0.005066 0.004925 0.099768 0.084527
Endosome 0.022332 0.151068 0.151687 0.063054 0.144654 0.141656 0.165860 0.106635 0.125970 0.146400 0.162918 0.086874
Golgi 0.000726 0.011360 0.001625 0.006541 0.005257 0.003693 0.011425 0.008258 0.004565 0.007558 0.009391 0.007479
Lipid Particles 0.019049 0.002996 0.013447 0.075863 0.005607 0.002433 0.075433 0.050776 0.007660 0.002717 0.062566 0.062151
Mitochondria 0.000245 0.002213 0.000407 0.001067 0.005280 0.003218 0.001455 0.003992 0.004511 0.002711 0.001237 0.002666
Mitotic Spindle 0.000084 0.000253 0.006032 0.000752 0.000251 0.000111 0.000820 0.003801 0.000226 0.000183 0.001902 0.002419
None 0.000359 0.000459 0.000562 0.000087 0.004050 0.000088 0.000120 0.000231 0.003486 0.000275 0.000211 0.000166
Nuclear Periphery 0.000612 0.000214 0.002868 0.000184 0.000829 0.001354 0.000319 0.000337 0.000796 0.000780 0.000848 0.000267
Nuclear Periphery Foci 0.001540 0.001049 0.020096 0.007727 0.001099 0.001098 0.009665 0.007968 0.001167 0.001073 0.011830 0.007859
Nucleolus 0.000371 0.000800 0.001787 0.000153 0.003824 0.000768 0.000262 0.000232 0.003296 0.000784 0.000579 0.000196
Nucleus 0.088597 0.004026 0.053325 0.005404 0.034997 0.005435 0.005097 0.006576 0.043184 0.004725 0.015108 0.006045
Peroxisomes 0.003106 0.001784 0.007066 0.003273 0.002051 0.001073 0.002123 0.005077 0.002213 0.001431 0.003149 0.004259
Vacuole 0.366963* 0.590276* 0.484760* 0.429893* 0.562934* 0.684079* 0.375434* 0.395697* 0.533001* 0.636796* 0.398128* 0.411202*
Vacuole Periphery 0.000418 0.117560* 0.002811 0.005275 0.091647 0.092608 0.005535 0.004669 0.077712 0.105186* 0.004970 0.004943

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.16 -0.65 -6.44 -7.39 -6.29 1.47 -8.70 -7.95 -8.00 -2.29 0.98 -8.66 -10.62 -10.78 -4.87
Bud Neck 0.03 -4.74 -3.66 -7.24 2.35 3.77 1.90 2.82 -2.23 2.33 4.11 0.87 1.75 -6.30 2.20
Bud Site -1.41 -1.49 -6.29 -9.51 -8.52 3.57 -6.96 -8.52 -13.38 -3.09 0.88 -6.71 -11.62 -15.65 -6.60
Cell Periphery -2.68 1.63 -9.98 -9.46 -10.13 0.85 -7.36 -5.00 -5.43 0.75 0.99 -7.01 -10.34 -10.84 -5.43
Cytoplasm 8.48 5.62 5.06 -13.94 -1.98 4.44 -7.01 -13.74 -23.06 -9.37 6.56 -1.97 -7.47 -25.60 -9.36
Cytoplasmic Foci 5.74 4.27 5.88 0.57 7.23 3.89 -2.86 -7.75 -15.51 -7.41 4.66 0.31 -0.64 -10.50 -2.01
Eisosomes 5.24 4.18 -1.65 -2.51 -2.34 2.93 -8.47 -8.23 -9.25 -2.51 6.12 -6.13 -3.28 -3.82 -2.37
Endoplasmic Reticulum -0.54 -5.05 -14.96 -15.04 -13.27 -0.53 -21.42 -12.59 -12.78 7.42 0.08 -21.15 -18.68 -19.72 3.16
Endosome -15.33 -11.73 -7.95 10.97 7.59 0.19 -1.65 3.68 4.35 9.63 -2.01 -3.07 4.44 10.24 15.05
Golgi -6.52 -1.15 -7.71 3.04 -6.16 0.85 -3.17 -1.40 -4.29 3.29 -1.77 -2.90 -1.64 0.49 2.71
Lipid Particles 1.92 0.68 -5.87 -14.19 -12.13 2.55 -20.19 -11.87 -13.38 5.49 2.86 -17.81 -15.86 -19.35 0.56
Mitochondria -5.38 -1.78 -2.09 2.39 -1.59 1.24 2.68 0.80 -0.34 -1.76 1.39 2.70 1.43 0.24 -1.70
Mitotic Spindle -3.27 -2.75 -2.97 -2.29 2.42 1.14 -0.78 -3.59 -4.17 -3.38 0.92 -2.51 -3.55 -4.46 -1.33
None -0.38 -0.83 2.93 1.55 2.07 1.76 1.74 1.69 -1.48 -1.11 1.68 1.71 1.74 0.83 0.69
Nuclear Periphery 2.72 -7.43 2.92 0.61 9.37 -0.92 1.98 1.86 1.96 -0.70 0.08 -0.87 2.42 1.96 8.63
Nuclear Periphery Foci 0.68 -7.46 -8.89 -9.96 5.09 0.04 -10.80 -7.12 -7.17 1.41 0.23 -12.98 -10.86 -11.05 4.30
Nucleolus -2.23 -4.52 2.81 3.84 5.14 2.30 2.69 2.71 4.33 1.00 2.23 2.31 2.76 5.59 5.39
Nucleus 4.56 1.42 4.46 -1.73 9.40 4.33 4.39 4.18 -1.00 -1.67 5.99 3.95 5.74 -1.92 7.46
Peroxisomes 1.10 -2.14 0.04 -2.67 3.43 2.28 -0.11 -4.46 -7.58 -5.47 1.81 -1.66 -3.71 -7.36 -2.96
Vacuole -5.35 -2.78 -1.63 10.39 3.17 -6.24 10.09 8.25 21.08 -2.41 -5.91 7.80 6.60 21.78 -2.23
Vacuole Periphery -20.21* -8.74 -8.83 19.35 -4.65 -0.16 11.21 11.32 18.79 1.76 -3.64 10.87 10.87 26.73 0.24