ORF
Human Ortholog
Description Similar to 6-phosphofructo-2-kinase enzymes; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 1.0 0.96 0.98 1.0 0.97 0.9 0.79 0.75 0.73 0.69 0.58 0.65 1.0 0.99 0.99 0.94 0.94 0.9 0.94 0.92 0.94 0.93 0.96 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.1 0.07 0.15 0.26 0.22 0.35 0.38 0.48 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.1 0.05 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 2 4 0 1 2 2 3 4 6 3 3 1 0 0 2 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 1 2 3 1 4 9 12 19 3 11 4 4 10 11 0 0 0 0 0 0
Cytoplasm 41 260 180 69 117 209 204 239 151 174 176 193 204 383 368 357 276 176 61 253 178 147 217 182
Endoplasmic Reticulum 0 1 0 0 0 1 3 2 2 3 1 7 0 1 4 9 4 6 0 0 1 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 6 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 9 7 7 8 35 68 70 72 96 146 122 0 1 0 10 0 1 0 4 1 0 0 1
Nucleus 0 1 1 0 0 3 0 3 1 3 7 2 0 0 2 17 20 21 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 3 0 2 7 2 1 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Vac/Vac Membrane 0 3 1 0 2 2 12 32 10 18 25 7 0 0 0 5 2 2 0 3 3 2 0 3
Unique Cell Count 41 272 183 69 121 231 258 317 206 251 303 299 204 385 373 378 295 195 65 277 189 158 227 198
Labelled Cell Count 43 278 193 76 129 254 295 350 246 316 373 356 208 396 378 405 314 218 65 277 189 158 227 198


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.2 5.2 5.3 5.8 5.8 5.2 5.8 5.8 5.3 5.5 5.3 7.2 7.9 8.0 8.5 10.7 10.9 6.0 6.5 7.7
Std Deviation (1e-4) 0.9 0.9 0.7 1.0 1.0 1.2 1.1 1.3 1.6 1.2 1.4 1.2 1.3 1.1 1.1 1.7 2.0 1.9 1.4 1.3 1.6
Intensity Change (Log2) – – – 0.01 0.16 0.15 0.0 0.15 0.16 0.01 0.07 0.03 0.47 0.59 0.62 0.71 1.03 1.06 0.2 0.31 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 3.5 0 0 0 0 0 3.3
Cytoplasm 1.1 -0.9 -3.3 -5.9 -6.7 -6.9 -7.7 -9.7 -8.6 1.8 1.3 0.3 -2.1 -2.4 -3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.1 3.8 6.2 5.4 7.6 8.3 10.2 8.9 0 0 0 -0.8 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 2.5 3.2 4.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.5 4.1 2.6 3.3 3.7 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3483 3.065 2.9091 2.5069 2.4633 2.9858 3.5607 3.8259 3.641 2.9951 3.0254 3.6964 3.4472 3.918 3.6496 3.2943 3.8765 3.5499
Actin 0.0042 0.0011 0.0005 0.0006 0.0209 0.0011 0.0331 0.0004 0.0141 0.0022 0.0034 0.0083 0.042 0.0007 0.0035 0.0236 0.0005 0.0005
Bud 0.0007 0.0001 0.0001 0.0002 0.0002 0.0003 0.0019 0.0002 0.0011 0.0004 0.001 0.0007 0.001 0.0002 0.0008 0.0004 0 0
Bud Neck 0.0028 0.0001 0.0002 0.0044 0.0375 0.0007 0.0022 0.0001 0.0005 0.0004 0.0005 0.0034 0.0019 0.0001 0.0001 0.0007 0.0002 0.0003
Bud Periphery 0.001 0.0001 0.0001 0.0003 0.0004 0.0003 0.0058 0.0001 0.0011 0.0004 0.0021 0.0009 0.0009 0.0001 0.0007 0.0006 0 0
Bud Site 0.0089 0.0004 0.0003 0.0023 0.0021 0.0001 0.0108 0.0008 0.0065 0.0004 0.0055 0.0066 0.0484 0.0016 0.0013 0.0006 0.0001 0
Cell Periphery 0.0013 0.0002 0.0002 0.0011 0.0027 0.0001 0.001 0.0001 0.0002 0 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.522 0.8605 0.8048 0.8164 0.803 0.8458 0.6454 0.8258 0.7475 0.686 0.6327 0.7654 0.6603 0.7898 0.8089 0.7966 0.783 0.7675
Cytoplasmic Foci 0.0325 0.0032 0.0024 0.0282 0.0146 0.0052 0.0315 0.0048 0.0301 0.0154 0.0568 0.0131 0.0262 0.0182 0.0102 0.0108 0.0062 0.0098
Eisosomes 0.0001 0 0 0.0001 0.0045 0 0.0007 0.0001 0.0001 0 0.0001 0 0.0005 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0041 0.004 0.005 0.0075 0.0115 0.0047 0.006 0.0027 0.0037 0.0012 0.0034 0.0019 0.0154 0.0033 0.0038 0.0072 0.0044 0.0021
Endosome 0.0913 0.0009 0.0009 0.0135 0.0072 0.0039 0.0267 0.0031 0.0241 0.0192 0.033 0.0059 0.0566 0.0028 0.003 0.0044 0.0035 0.0014
Golgi 0.0634 0.0001 0.0002 0.0014 0.0057 0.0004 0.0043 0.0003 0.0135 0.0178 0.0213 0.0019 0.012 0.0007 0.0021 0.0044 0.0002 0.0003
Lipid Particles 0.0179 0.0001 0.0002 0.0062 0.0093 0.0001 0.0052 0.0002 0.0045 0.0008 0.0121 0.0008 0.0311 0.0014 0.0008 0.006 0.0003 0.0019
Mitochondria 0.0141 0.0002 0.0242 0.0007 0.0006 0.0009 0.0074 0.0001 0.0162 0.0059 0.036 0.0003 0.0035 0.0001 0.0164 0.0008 0.0001 0.0001
None 0.1915 0.1235 0.1581 0.1056 0.0679 0.1293 0.1894 0.1569 0.1138 0.2447 0.1239 0.1813 0.0693 0.1724 0.1409 0.1235 0.197 0.2073
Nuclear Periphery 0.0176 0.0018 0.0006 0.0011 0.0007 0.0011 0.0054 0.0005 0.0014 0.0005 0.0031 0.0004 0.0101 0.0011 0.0009 0.0016 0.0012 0.0004
Nucleolus 0.0009 0 0 0.0005 0.0039 0.0001 0.0004 0 0.0001 0 0.0002 0 0.0003 0.0001 0 0.0002 0 0
Nucleus 0.0028 0.0022 0.0011 0.0018 0.0022 0.004 0.0049 0.0021 0.0022 0.0014 0.005 0.0016 0.0023 0.0017 0.0011 0.0041 0.0017 0.0019
Peroxisomes 0.0077 0.0001 0 0.001 0.001 0.0002 0.01 0.0001 0.0161 0.0017 0.0514 0.0058 0.0084 0.0007 0.0041 0.0114 0.0001 0.0047
Punctate Nuclear 0.0032 0.0003 0.0002 0.0008 0.0026 0.0007 0.0032 0.0005 0.0012 0.0004 0.0052 0.0012 0.0036 0.0042 0.0006 0.0012 0.001 0.0013
Vacuole 0.0065 0.0008 0.0007 0.006 0.0012 0.001 0.0033 0.0011 0.0014 0.0008 0.0022 0.0003 0.0042 0.0007 0.0005 0.0014 0.0005 0.0003
Vacuole Periphery 0.0055 0.0001 0.0001 0.0004 0.0002 0.0002 0.0014 0.0001 0.0007 0.0003 0.0008 0.0001 0.0015 0.0001 0.0001 0.0003 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2118 7.7363 5.7407 13.5738 7.0128 8.6494 14.9925 15.3983 15.9608 12.576
Translational Efficiency 0.7893 0.7349 1.1828 0.7117 0.7975 1.3774 0.842 0.7596 1.0353 0.9332

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘