ORF | |
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Human Ortholog | |
Description | Similar to 6-phosphofructo-2-kinase enzymes; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 4 | 0 | 1 | 2 | 2 | 3 | 4 | 6 | 3 | 3 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 1 | 2 | 3 | 1 | 4 | 9 | 12 | 19 | 3 | 11 | 4 | 4 | 10 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 41 | 260 | 180 | 69 | 117 | 209 | 204 | 239 | 151 | 174 | 176 | 193 | 204 | 383 | 368 | 357 | 276 | 176 | 61 | 253 | 178 | 147 | 217 | 182 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 3 | 1 | 7 | 0 | 1 | 4 | 9 | 4 | 6 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 6 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 9 | 7 | 7 | 8 | 35 | 68 | 70 | 72 | 96 | 146 | 122 | 0 | 1 | 0 | 10 | 0 | 1 | 0 | 4 | 1 | 0 | 0 | 1 |
Nucleus | 0 | 1 | 1 | 0 | 0 | 3 | 0 | 3 | 1 | 3 | 7 | 2 | 0 | 0 | 2 | 17 | 20 | 21 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 7 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 3 | 1 | 0 | 2 | 2 | 12 | 32 | 10 | 18 | 25 | 7 | 0 | 0 | 0 | 5 | 2 | 2 | 0 | 3 | 3 | 2 | 0 | 3 |
Unique Cell Count | 41 | 272 | 183 | 69 | 121 | 231 | 258 | 317 | 206 | 251 | 303 | 299 | 204 | 385 | 373 | 378 | 295 | 195 | 65 | 277 | 189 | 158 | 227 | 198 |
Labelled Cell Count | 43 | 278 | 193 | 76 | 129 | 254 | 295 | 350 | 246 | 316 | 373 | 356 | 208 | 396 | 378 | 405 | 314 | 218 | 65 | 277 | 189 | 158 | 227 | 198 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 6.2 | 5.2 | 5.3 | 5.8 | 5.8 | 5.2 | 5.8 | 5.8 | 5.3 | 5.5 | 5.3 | 7.2 | 7.9 | 8.0 | 8.5 | 10.7 | 10.9 | 6.0 | 6.5 | 7.7 |
Std Deviation (1e-4) | 0.9 | 0.9 | 0.7 | 1.0 | 1.0 | 1.2 | 1.1 | 1.3 | 1.6 | 1.2 | 1.4 | 1.2 | 1.3 | 1.1 | 1.1 | 1.7 | 2.0 | 1.9 | 1.4 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.16 | 0.15 | 0.0 | 0.15 | 0.16 | 0.01 | 0.07 | 0.03 | 0.47 | 0.59 | 0.62 | 0.71 | 1.03 | 1.06 | 0.2 | 0.31 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 3.3 |
Cytoplasm | 1.1 | -0.9 | -3.3 | -5.9 | -6.7 | -6.9 | -7.7 | -9.7 | -8.6 | 1.8 | 1.3 | 0.3 | -2.1 | -2.4 | -3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.1 | 3.8 | 6.2 | 5.4 | 7.6 | 8.3 | 10.2 | 8.9 | 0 | 0 | 0 | -0.8 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 3.2 | 4.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.5 | 4.1 | 2.6 | 3.3 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3483 | 3.065 | 2.9091 | 2.5069 | 2.4633 | 2.9858 | 3.5607 | 3.8259 | 3.641 | 2.9951 | 3.0254 | 3.6964 | 3.4472 | 3.918 | 3.6496 | 3.2943 | 3.8765 | 3.5499 |
Actin | 0.0042 | 0.0011 | 0.0005 | 0.0006 | 0.0209 | 0.0011 | 0.0331 | 0.0004 | 0.0141 | 0.0022 | 0.0034 | 0.0083 | 0.042 | 0.0007 | 0.0035 | 0.0236 | 0.0005 | 0.0005 |
Bud | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0019 | 0.0002 | 0.0011 | 0.0004 | 0.001 | 0.0007 | 0.001 | 0.0002 | 0.0008 | 0.0004 | 0 | 0 |
Bud Neck | 0.0028 | 0.0001 | 0.0002 | 0.0044 | 0.0375 | 0.0007 | 0.0022 | 0.0001 | 0.0005 | 0.0004 | 0.0005 | 0.0034 | 0.0019 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0003 |
Bud Periphery | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0058 | 0.0001 | 0.0011 | 0.0004 | 0.0021 | 0.0009 | 0.0009 | 0.0001 | 0.0007 | 0.0006 | 0 | 0 |
Bud Site | 0.0089 | 0.0004 | 0.0003 | 0.0023 | 0.0021 | 0.0001 | 0.0108 | 0.0008 | 0.0065 | 0.0004 | 0.0055 | 0.0066 | 0.0484 | 0.0016 | 0.0013 | 0.0006 | 0.0001 | 0 |
Cell Periphery | 0.0013 | 0.0002 | 0.0002 | 0.0011 | 0.0027 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.522 | 0.8605 | 0.8048 | 0.8164 | 0.803 | 0.8458 | 0.6454 | 0.8258 | 0.7475 | 0.686 | 0.6327 | 0.7654 | 0.6603 | 0.7898 | 0.8089 | 0.7966 | 0.783 | 0.7675 |
Cytoplasmic Foci | 0.0325 | 0.0032 | 0.0024 | 0.0282 | 0.0146 | 0.0052 | 0.0315 | 0.0048 | 0.0301 | 0.0154 | 0.0568 | 0.0131 | 0.0262 | 0.0182 | 0.0102 | 0.0108 | 0.0062 | 0.0098 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0.0045 | 0 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0041 | 0.004 | 0.005 | 0.0075 | 0.0115 | 0.0047 | 0.006 | 0.0027 | 0.0037 | 0.0012 | 0.0034 | 0.0019 | 0.0154 | 0.0033 | 0.0038 | 0.0072 | 0.0044 | 0.0021 |
Endosome | 0.0913 | 0.0009 | 0.0009 | 0.0135 | 0.0072 | 0.0039 | 0.0267 | 0.0031 | 0.0241 | 0.0192 | 0.033 | 0.0059 | 0.0566 | 0.0028 | 0.003 | 0.0044 | 0.0035 | 0.0014 |
Golgi | 0.0634 | 0.0001 | 0.0002 | 0.0014 | 0.0057 | 0.0004 | 0.0043 | 0.0003 | 0.0135 | 0.0178 | 0.0213 | 0.0019 | 0.012 | 0.0007 | 0.0021 | 0.0044 | 0.0002 | 0.0003 |
Lipid Particles | 0.0179 | 0.0001 | 0.0002 | 0.0062 | 0.0093 | 0.0001 | 0.0052 | 0.0002 | 0.0045 | 0.0008 | 0.0121 | 0.0008 | 0.0311 | 0.0014 | 0.0008 | 0.006 | 0.0003 | 0.0019 |
Mitochondria | 0.0141 | 0.0002 | 0.0242 | 0.0007 | 0.0006 | 0.0009 | 0.0074 | 0.0001 | 0.0162 | 0.0059 | 0.036 | 0.0003 | 0.0035 | 0.0001 | 0.0164 | 0.0008 | 0.0001 | 0.0001 |
None | 0.1915 | 0.1235 | 0.1581 | 0.1056 | 0.0679 | 0.1293 | 0.1894 | 0.1569 | 0.1138 | 0.2447 | 0.1239 | 0.1813 | 0.0693 | 0.1724 | 0.1409 | 0.1235 | 0.197 | 0.2073 |
Nuclear Periphery | 0.0176 | 0.0018 | 0.0006 | 0.0011 | 0.0007 | 0.0011 | 0.0054 | 0.0005 | 0.0014 | 0.0005 | 0.0031 | 0.0004 | 0.0101 | 0.0011 | 0.0009 | 0.0016 | 0.0012 | 0.0004 |
Nucleolus | 0.0009 | 0 | 0 | 0.0005 | 0.0039 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0 | 0.0002 | 0 | 0 |
Nucleus | 0.0028 | 0.0022 | 0.0011 | 0.0018 | 0.0022 | 0.004 | 0.0049 | 0.0021 | 0.0022 | 0.0014 | 0.005 | 0.0016 | 0.0023 | 0.0017 | 0.0011 | 0.0041 | 0.0017 | 0.0019 |
Peroxisomes | 0.0077 | 0.0001 | 0 | 0.001 | 0.001 | 0.0002 | 0.01 | 0.0001 | 0.0161 | 0.0017 | 0.0514 | 0.0058 | 0.0084 | 0.0007 | 0.0041 | 0.0114 | 0.0001 | 0.0047 |
Punctate Nuclear | 0.0032 | 0.0003 | 0.0002 | 0.0008 | 0.0026 | 0.0007 | 0.0032 | 0.0005 | 0.0012 | 0.0004 | 0.0052 | 0.0012 | 0.0036 | 0.0042 | 0.0006 | 0.0012 | 0.001 | 0.0013 |
Vacuole | 0.0065 | 0.0008 | 0.0007 | 0.006 | 0.0012 | 0.001 | 0.0033 | 0.0011 | 0.0014 | 0.0008 | 0.0022 | 0.0003 | 0.0042 | 0.0007 | 0.0005 | 0.0014 | 0.0005 | 0.0003 |
Vacuole Periphery | 0.0055 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0014 | 0.0001 | 0.0007 | 0.0003 | 0.0008 | 0.0001 | 0.0015 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.2118 | 7.7363 | 5.7407 | 13.5738 | 7.0128 | 8.6494 | 14.9925 | 15.3983 | 15.9608 | 12.576 |
Translational Efficiency | 0.7893 | 0.7349 | 1.1828 | 0.7117 | 0.7975 | 1.3774 | 0.842 | 0.7596 | 1.0353 | 0.9332 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |