Standard name
Description Mitochondrial dicarboxylate carrier; integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.07 0 0 0.05 0.06 0.06 – 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.1 0.08 0.08 0.09 0 0.06 0 0 – 0.05 0.06 0.05 0.1 0.06 0.09 0.59 0.5 0.56 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.07 0.09 0 0 0 0 0 0
Endosome 0 0 0 0.05 0 0 0 0 – 0 0 0 0.14 0.22 0.14 0.1 0.13 0.08 0 0.08 0.09 0.07 0.06 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0.1 0.09 0.24 0 0 0 0 0 0 0 0 0.06
Mitochondria 0.89 0.74 0.86 0.88 0.9 0.92 0.95 0.93 – 0.94 0.95 0.95 0.53 0.49 0.43 0.12 0.1 0.07 0.82 0.71 0.74 0.67 0.6 0.59
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 – 0 0 0 0.08 0.09 0.06 0 0.06 0.05 0 0 0.06 0 0.05 0.05
SpindlePole 0 0 0.06 0 0 0 0 0 – 0.07 0.05 0 0 0 0 0.08 0.05 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0.2 0 0.05 0.07 0.07 0 0.05 – 0.05 0 0 0.25 0.29 0.28 0.05 0.1 0.08 0 0 0 0.07 0.1 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 – 1 0 1 1 0 3 4 5 6 0 4 6 3 6 11
Bud 5 1 23 7 10 25 41 42 – 32 27 31 6 2 4 4 4 9 3 1 3 3 4 4
Bud Neck 0 0 1 0 0 0 1 1 – 1 1 1 0 0 0 4 4 1 0 1 1 3 4 7
Bud Site 2 0 2 0 3 14 10 11 – 13 12 8 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 12 4 3 1 2 0 1 – 1 2 4 4 9 10 17 17 14 0 0 0 0 0 0
Cytoplasm 12 15 25 19 18 30 16 29 – 28 27 27 25 19 28 116 117 126 0 2 2 4 3 3
Endoplasmic Reticulum 0 8 0 0 3 0 1 2 – 0 0 1 2 0 6 13 16 20 0 1 1 3 2 3
Endosome 0 8 12 11 19 4 1 1 – 1 0 0 34 72 47 19 30 18 3 16 29 18 14 12
Golgi 0 8 0 1 0 0 0 0 – 0 0 0 24 31 78 5 5 4 2 7 3 5 8 15
Mitochondria 105 138 284 192 383 496 601 702 – 533 466 534 129 162 140 23 23 15 103 153 253 169 141 170
Nucleus 4 1 1 2 2 22 23 23 – 13 8 9 4 3 1 2 1 1 0 3 0 0 2 1
Nuclear Periphery 0 2 1 1 10 5 8 10 – 6 7 6 1 0 0 0 1 0 0 1 4 2 1 1
Nucleolus 1 1 1 0 10 18 17 17 – 4 12 12 1 1 1 0 0 0 0 0 0 0 2 0
Peroxisomes 0 4 22 7 7 6 8 6 – 7 5 0 19 30 19 3 14 12 5 7 21 9 11 14
SpindlePole 1 4 20 5 14 20 28 32 – 38 24 19 8 12 2 15 12 17 2 1 3 5 4 5
Vac/Vac Membrane 0 38 13 10 28 35 22 35 – 26 13 13 60 95 91 10 24 18 1 9 5 16 22 30
Unique Cell Count 118 187 331 217 425 537 633 753 567 488 560 243 332 328 198 234 224 127 215 345 254 235 287
Labelled Cell Count 130 241 409 258 508 677 777 912 704 604 666 318 437 430 235 273 261 127 215 345 254 235 287


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.3 4.0 4.5 4.4 3.8 3.3 3.3 – 3.1 3.3 3.2 6.5 7.6 7.4 5.4 5.7 5.6 6.4 6.7 6.5
Std Deviation (1e-4) 1.4 0.9 0.7 2.0 1.4 1.0 0.9 0.8 – 0.7 1.2 0.8 1.3 9.7 1.6 1.1 1.3 1.3 1.9 1.9 1.4
Intensity Change (Log2) – – – 0.17 0.15 -0.06 -0.26 -0.27 – -0.37 -0.3 -0.33 0.71 0.92 0.87 0.43 0.5 0.49 0.68 0.73 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 -2.4 -4.3 -3.7 -2.5 -2.9 -1.5
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 1.4 1.6 4.2 3.7 3.3
Cytoplasm 0.5 -2.0 -1.2 -3.7 -2.6 – -1.6 -1.2 -1.7 1.1 -0.9 0.5 12.8 11.5 12.6
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 4.8 5.5
Endosome 0 0 0 0 0 – 0 0 0 4.5 7.0 4.8 2.8 4.1 2.3
Golgi 0 0 0 0 0 – 0 0 0 5.8 5.7 9.4 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -8.6 -10.2 -11.6 -16.7 -17.9 -18.3
Nucleus 0 0 3.4 3.2 2.8 – 2.3 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0.5 1.1 -0.5 -2.7 -0.3 -0.6
SpindlePole 0 0 0 0 0 – 0 0 0 -1.5 -1.5 -3.9 0.7 -0.5 0.7
Vacuole 0.4 1.6 1.6 -0.4 0.5 – 0.5 -1.0 -1.4 7.4 8.6 8.4 0.6 3.0 2.1