Standard name
Human Ortholog
Description Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.97 0.97 0.95 0.84 0.78 0.76 0.68 0.66 0.53 0.98 0.99 1.0 0.81 0.84 0.69 0.77 0.8 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.08 0.1 0.32 0.4 0.36 0.53 0.57 0.66 0 0 0 0.08 0.07 0.15 0.1 0.09 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 4 3 7 2 2 3
Bud 0 2 1 1 1 3 11 6 10 10 13 0 0 0 8 5 7 0 5 8
Bud Neck 2 1 4 2 4 0 4 9 2 4 2 0 0 0 2 1 2 1 2 5
Bud Site 0 0 0 0 0 2 2 0 3 1 1 0 0 0
Cell Periphery 2 1 2 2 0 1 0 1 1 1 0 2 0 0 1 1 2 3 4 2
Cytoplasm 421 384 372 255 346 366 453 361 356 280 264 125 272 250 346 332 264 215 258 302
Endoplasmic Reticulum 1 2 1 1 0 0 0 0 0 0 3 0 0 1 0 0 4 2 1 1
Endosome 2 0 1 0 2 1 0 2 1 0 0 0 1 0 5 3 4 1 1 2
Golgi 2 0 1 0 0 0 0 0 0 0 0 0 0 0 3 0 2 3 0 0
Mitochondria 7 21 12 21 38 138 232 174 277 243 325 0 0 0 33 28 55 28 28 23
Nucleus 0 0 0 1 0 2 0 3 1 2 1 1 1 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 1 1 1 2 0 3 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 1 0 0 0 0 2 0 0 0 1 0 1 0 0 0
SpindlePole 1 1 2 1 1 7 11 7 16 13 16 1 0 0 8 2 7 3 3 6
Vac/Vac Membrane 2 1 2 0 3 3 7 3 0 1 2 0 0 0 4 4 7 6 4 7
Unique Cell Count 434 397 385 263 364 436 581 477 526 427 494 127 274 251 430 394 381 280 323 374
Labelled Cell Count 441 415 398 284 397 525 721 568 670 558 629 129 274 252 430 394 381 280 323 374


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.4 3.4 3.2 3.1 2.6 2.4 3.0 2.2 2.2 2.4 4.4 5.0 4.9 3.9 4.0 4.6 4.9 4.9 5.1
Std Deviation (1e-4) 0.8 0.5 1.6 1.3 1.1 1.0 0.9 1.9 1.0 0.6 0.9 0.8 1.5 1.0 1.2 0.9 1.3 1.5 1.3 1.4
Intensity Change (Log2) -0.12 -0.12 -0.42 -0.52 -0.19 -0.62 -0.64 -0.53 0.37 0.55 0.51 0.18 0.21 0.43 0.5 0.52 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0793 0.0013 0.0325 0.0037 0.0065 0.0074 0.0271 0.0001 0.0252 0.0043 0.0017 0.0006
Bud 0.0032 0.0025 0.0064 0.0009 0.0003 0.0079 0.0011 0.0002 0.0029 0.0007 0.001 0.0001
Bud Neck 0.0075 0.0001 0.0039 0.0003 0.0002 0.0071 0.0039 0.0001 0.0006 0.0005 0 0.0001
Bud Periphery 0.0076 0.0054 0.0086 0.0055 0.0011 0.0018 0.0038 0.0003 0.0033 0.0045 0.0028 0.0001
Bud Site 0.0088 0.004 0.0073 0.0007 0.0003 0.0009 0.0055 0.0014 0.0075 0.0014 0.0001 0
Cell Periphery 0.001 0.0003 0.0018 0.0004 0.0001 0.0001 0.0014 0.0001 0.0002 0.0005 0.0001 0
Cytoplasm 0.0188 0.0247 0.0299 0.0172 0.0369 0.0289 0.0224 0.024 0.0307 0.0696 0.0367 0.0184
Cytoplasmic Foci 0.0208 0.0048 0.006 0.0025 0.0154 0.005 0.0449 0.0016 0.004 0.0023 0.0067 0.0024
Eisosomes 0.0009 0.0003 0.0004 0.0004 0.0001 0.0001 0.0007 0.0002 0.0003 0.0008 0.0002 0.0001
Endoplasmic Reticulum 0.0041 0.001 0.0015 0.0028 0.002 0.0004 0.0171 0.0003 0.001 0.0006 0.0005 0.0005
Endosome 0.0192 0.0017 0.0044 0.0039 0.0216 0.0018 0.0363 0.0001 0.0024 0.0004 0.0011 0.0053
Golgi 0.0163 0.0003 0.0032 0.0026 0.0226 0.0015 0.0072 0 0.0024 0.0001 0.0002 0.0009
Lipid Particles 0.0195 0.0005 0.0024 0.0054 0.0046 0.0022 0.0224 0.0001 0.0029 0.0002 0.0042 0.0002
Mitochondria 0.0144 0.0011 0.0071 0.0191 0.0269 0.0004 0.004 0.0002 0.0093 0.0004 0.0004 0.0003
None 0.7334 0.9414 0.8684 0.9289 0.8566 0.93 0.7406 0.9702 0.8927 0.9118 0.9394 0.9698
Nuclear Periphery 0.0143 0.0009 0.0017 0.0007 0.0011 0.0004 0.0238 0.0001 0.0013 0.0003 0.0013 0.0003
Nucleolus 0.0021 0.0009 0.0022 0.0004 0.0001 0.0008 0.0017 0.0002 0.0003 0.0002 0.0002 0
Nucleus 0.0114 0.0032 0.0038 0.0004 0.0002 0.0014 0.0034 0.0003 0.0037 0.0003 0.0002 0.0001
Peroxisomes 0.0062 0.0002 0.003 0.0019 0.0014 0.0007 0.0158 0.0001 0.0072 0.0005 0.0007 0.0001
Punctate Nuclear 0.0068 0.004 0.0027 0.0003 0.0005 0.0007 0.0096 0.0003 0.0005 0.0003 0.0019 0.0002
Vacuole 0.0032 0.0011 0.0024 0.0014 0.001 0.0006 0.0042 0.0002 0.0011 0.0003 0.0002 0.0003
Vacuole Periphery 0.0014 0.0002 0.0005 0.0006 0.0005 0.0001 0.0031 0 0.0004 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.7221 50.1408 71.6685 84.0963 48.5812 32.1648 53.2945 59.0388 61.0499 64.3821
Translational Efficiency 0.9027 0.9706 1.0487 0.9829 0.9945 1.0549 0.7817 0.8198 0.9065 0.7503

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
426 1441 1205 1312 1530 1841 1551 1854 1956 3282 2756 3166

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 786.38 707.90 796.92 784.08 609.01 691.78 719.49 820.03 647.64 698.86 753.34 805.13
Standard Deviation 127.69 92.17 102.05 140.57 61.52 85.72 94.57 126.73 108.95 88.97 105.17 133.82
Intensity Change Log 2 -0.151681 0.019208 -0.004226 0.183847 0.240509 0.429211 0.004429 0.119991 0.201105

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001980 0.000270 0.000234 0.004456 0.000105 0.000335 0.000218 0.001141 0.000513 0.000306 0.000225 0.002515
Bud Neck 0.004903 0.028015 0.002980 0.007491 0.020404 0.051716 0.004780 0.010934 0.017028 0.041310 0.003993 0.009508
Bud Site 0.043962 0.008659 0.007481 0.031495 0.002855 0.013136 0.008279 0.034557 0.011808 0.011170 0.007930 0.033288
Cell Periphery 0.000346 0.000122 0.000202 0.000638 0.000144 0.000403 0.000246 0.000650 0.000188 0.000280 0.000227 0.000645
Cytoplasm 0.374887* 0.478119* 0.273501* 0.446757* 0.431756* 0.337181* 0.460111* 0.483193* 0.419371* 0.399062* 0.378520* 0.468094*
Cytoplasmic Foci 0.069503 0.216890* 0.014751 0.046226 0.261310* 0.240670* 0.015474 0.033108 0.219536* 0.230229* 0.015158 0.038544
Eisosomes 0.000102 0.000140 0.000049 0.000159 0.000266 0.000285 0.000038 0.000217 0.000231 0.000221 0.000043 0.000193
Endoplasmic Reticulum 0.002219 0.000571 0.007657 0.019732 0.000774 0.001130 0.009957 0.031506 0.001088 0.000885 0.008952 0.026627
Endosome 0.017694 0.017676 0.003913 0.013276 0.011690 0.034467 0.001291 0.006960 0.012998 0.027095 0.002437 0.009577
Golgi 0.018126 0.003134 0.000814 0.006034 0.002038 0.005696 0.000166 0.002410 0.005542 0.004571 0.000449 0.003912
Lipid Particles 0.003449 0.008504 0.002709 0.004794 0.019208 0.025613 0.001087 0.003512 0.015775 0.018101 0.001796 0.004043
Mitochondria 0.010066 0.006720 0.000586 0.007385 0.006930 0.020051 0.000646 0.003440 0.007613 0.014198 0.000620 0.005074
Mitotic Spindle 0.024209 0.002946 0.003372 0.040558 0.000354 0.002484 0.004258 0.032476 0.005549 0.002687 0.003871 0.035825
None 0.001431 0.004673 0.005677 0.004707 0.003549 0.004249 0.004816 0.002783 0.003088 0.004435 0.005192 0.003580
Nuclear Periphery 0.000929 0.000416 0.002931 0.002500 0.000737 0.000905 0.001451 0.002124 0.000778 0.000690 0.002098 0.002279
Nuclear Periphery Foci 0.001133 0.000372 0.002487 0.001994 0.000197 0.000272 0.001195 0.002266 0.000401 0.000316 0.001760 0.002153
Nucleolus 0.000816 0.002440 0.000945 0.000741 0.001313 0.002650 0.000317 0.000835 0.001205 0.002557 0.000592 0.000796
Nucleus 0.212159* 0.145788* 0.631682* 0.213448* 0.186759* 0.161344* 0.445038* 0.230934* 0.192291* 0.154514* 0.526644* 0.223687*
Peroxisomes 0.002336 0.011821 0.000566 0.002765 0.009385 0.014964 0.000212 0.002125 0.007850 0.013584 0.000367 0.002390
Vacuole 0.207322* 0.061399 0.037175 0.141205 0.039341 0.079225 0.040271 0.112546 0.075926 0.071398 0.038917 0.124422
Vacuole Periphery 0.002426 0.001326 0.000289 0.003639 0.000885 0.003224 0.000147 0.002284 0.001221 0.002391 0.000209 0.002845

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.59 2.59 -1.34 -2.49 -2.48 -2.90 -3.59 -2.90 -1.76 -2.24 0.55 1.14 -2.76 -2.93 -3.05
Bud Neck -11.12 1.15 -2.59 10.35 -4.93 -10.35 9.24 6.75 15.58 -4.10 -11.60 9.87 6.84 18.48 -5.93
Bud Site 3.77 4.04 1.34 -5.19 -5.51 -6.57 -3.27 -9.59 -4.13 -6.78 -1.52 1.11 -6.96 -6.45 -8.74
Cell Periphery 2.76 1.02 -2.08 -4.52 -3.24 -5.22 -1.38 -5.28 -0.03 -3.18 -3.78 -1.28 -5.94 -2.66 -4.52
Cytoplasm -9.56 4.53 -4.76 6.71 -13.55 10.59 1.94 0.04 -10.38 -1.87 1.35 8.04 -1.29 -2.93 -10.09
Cytoplasmic Foci -22.40 8.85 4.17 30.69 -9.28 2.84 44.73 41.67 41.82 -7.61 -2.38 41.90 37.90 51.59 -11.90
Eisosomes -4.44 6.50 -1.21 0.68 -3.23 -0.98 14.14 1.76 2.06 -1.57 0.61 14.54 2.22 2.07 -2.23
Endoplasmic Reticulum 4.39 -3.49 -4.28 -5.08 -1.85 -2.21 -4.74 -6.85 -6.71 -3.38 1.51 -6.24 -8.26 -8.40 -3.86
Endosome -2.53 4.08 1.54 5.54 -4.40 -12.06 10.87 6.69 17.65 -6.79 -11.08 10.64 5.62 17.46 -7.23
Golgi 2.10 2.83 1.99 -0.26 -2.98 -5.53 7.03 1.62 6.21 -3.19 -0.82 4.42 1.94 4.20 -4.12
Lipid Particles -4.99 0.13 -0.66 4.31 -0.91 -3.31 12.77 11.48 17.23 -5.09 -1.70 12.23 10.92 16.52 -3.43
Mitochondria -0.29 2.25 0.57 1.65 -4.13 -7.41 6.99 4.71 11.24 -4.10 -5.78 7.01 3.60 10.38 -5.68
Mitotic Spindle 3.17 3.08 -2.00 -7.48 -7.40 -3.63 -3.53 -8.78 -7.85 -7.00 1.23 0.29 -9.49 -10.81 -10.13
None -5.42 -7.43 -5.71 0.73 1.52 -0.80 -1.06 1.25 2.15 4.67 -2.04 -2.84 -0.41 2.21 3.95
Nuclear Periphery 6.95 -4.01 -2.40 -4.50 0.74 -1.32 -6.78 -8.37 -7.42 -3.19 2.14 -7.38 -7.08 -7.94 -0.84
Nuclear Periphery Foci 2.67 -2.51 -1.60 -6.78 1.69 -1.37 -4.44 -4.93 -4.59 -1.74 0.90 -5.34 -6.27 -6.75 0.00
Nucleolus -4.07 -1.32 -0.14 4.65 3.14 -5.20 6.90 2.71 6.95 -1.87 -5.78 4.49 3.00 8.04 -0.59
Nucleus 5.59 -26.06 -1.65 -12.91 35.64 4.11 -33.30 -11.57 -15.34 21.58 7.30 -47.41 -10.64 -20.14 38.91
Peroxisomes -9.16 1.93 -0.11 8.98 -1.98 -4.18 11.11 8.88 12.38 -2.59 -6.01 11.08 8.51 15.30 -3.20
Vacuole 15.62 16.23 5.52 -20.57 -21.53 -11.50 -6.79 -24.81 -15.81 -18.34 5.13 7.85 -15.48 -25.48 -28.01
Vacuole Periphery 0.34 2.66 -1.05 -1.78 -4.26 -7.94 8.09 -1.28 4.68 -3.81 -6.56 6.69 -2.22 2.48 -5.69