Standard name | |
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Description | Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 4 | 3 | 7 | 2 | 2 | 3 |
Bud | 0 | 2 | 1 | 1 | 1 | 3 | 11 | 6 | – | 10 | 10 | 13 | 0 | 0 | 0 | – | – | – | 8 | 5 | 7 | 0 | 5 | 8 |
Bud Neck | 2 | 1 | 4 | 2 | 4 | 0 | 4 | 9 | – | 2 | 4 | 2 | 0 | 0 | 0 | – | – | – | 2 | 1 | 2 | 1 | 2 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | – | 3 | 1 | 1 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 2 | 2 | 0 | 1 | 0 | 1 | – | 1 | 1 | 0 | 2 | 0 | 0 | – | – | – | 1 | 1 | 2 | 3 | 4 | 2 |
Cytoplasm | 421 | 384 | 372 | 255 | 346 | 366 | 453 | 361 | – | 356 | 280 | 264 | 125 | 272 | 250 | – | – | – | 346 | 332 | 264 | 215 | 258 | 302 |
Endoplasmic Reticulum | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 0 | 0 | 1 | – | – | – | 0 | 0 | 4 | 2 | 1 | 1 |
Endosome | 2 | 0 | 1 | 0 | 2 | 1 | 0 | 2 | – | 1 | 0 | 0 | 0 | 1 | 0 | – | – | – | 5 | 3 | 4 | 1 | 1 | 2 |
Golgi | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 0 | 2 | 3 | 0 | 0 |
Mitochondria | 7 | 21 | 12 | 21 | 38 | 138 | 232 | 174 | – | 277 | 243 | 325 | 0 | 0 | 0 | – | – | – | 33 | 28 | 55 | 28 | 28 | 23 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 3 | – | 1 | 2 | 1 | 1 | 1 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 2 | – | 0 | 3 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 2 | 1 | 1 | 7 | 11 | 7 | – | 16 | 13 | 16 | 1 | 0 | 0 | – | – | – | 8 | 2 | 7 | 3 | 3 | 6 |
Vac/Vac Membrane | 2 | 1 | 2 | 0 | 3 | 3 | 7 | 3 | – | 0 | 1 | 2 | 0 | 0 | 0 | – | – | – | 4 | 4 | 7 | 6 | 4 | 7 |
Unique Cell Count | 434 | 397 | 385 | 263 | 364 | 436 | 581 | 477 | 526 | 427 | 494 | 127 | 274 | 251 | 430 | 394 | 381 | 280 | 323 | 374 | ||||
Labelled Cell Count | 441 | 415 | 398 | 284 | 397 | 525 | 721 | 568 | 670 | 558 | 629 | 129 | 274 | 252 | 430 | 394 | 381 | 280 | 323 | 374 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 3.9 | 3.4 | 3.4 | 3.2 | 3.1 | 2.6 | 2.4 | 3.0 | – | 2.2 | 2.2 | 2.4 | 4.4 | 5.0 | 4.9 | 3.9 | 4.0 | 4.6 | 4.9 | 4.9 | 5.1 |
Std Deviation (1e-4) | 0.8 | 0.5 | 1.6 | 1.3 | 1.1 | 1.0 | 0.9 | 1.9 | – | 1.0 | 0.6 | 0.9 | 0.8 | 1.5 | 1.0 | 1.2 | 0.9 | 1.3 | 1.5 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.12 | -0.42 | -0.52 | -0.19 | – | -0.62 | -0.64 | -0.53 | 0.37 | 0.55 | 0.51 | 0.18 | 0.21 | 0.43 | 0.5 | 0.52 | 0.57 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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