Standard name | |
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Human Ortholog | |
Description | Glycosylated integral membrane protein localized to plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles; VID22 has a paralog, ENV11, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 7 | – | 2 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 1 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 5 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 10 | 14 | 9 | – | 26 | 18 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 5 | 12 | 11 | 24 | 13 | 7 | 9 | – | 9 | 11 | 5 | 7 | 7 | 15 | 44 | 35 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 5 | 3 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 4 | 0 | 8 | 1 | 8 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 14 | 6 | 18 | 3 | 8 | 15 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 4 | 0 | 0 | 1 |
Mitochondria | 3 | 59 | 9 | 29 | 24 | 128 | 175 | 147 | – | 181 | 186 | 199 | 0 | 0 | 0 | 18 | 6 | 6 | 3 | 4 | 6 | 1 | 5 | 2 |
Nucleus | 457 | 232 | 233 | 174 | 180 | 226 | 202 | 261 | – | 208 | 170 | 225 | 32 | 32 | 43 | 293 | 217 | 84 | 368 | 214 | 167 | 28 | 65 | 74 |
Nuclear Periphery | 17 | 10 | 22 | 10 | 19 | 19 | 12 | 20 | – | 19 | 20 | 27 | 0 | 1 | 1 | 22 | 14 | 6 | 10 | 3 | 17 | 0 | 0 | 4 |
Nucleolus | 143 | 60 | 68 | 61 | 95 | 166 | 244 | 291 | – | 251 | 153 | 256 | 4 | 4 | 8 | 61 | 60 | 26 | 198 | 63 | 142 | 26 | 40 | 53 |
Peroxisomes | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 6 | 1 | 17 | 3 | 4 | 2 | 4 | 9 | – | 4 | 2 | 4 | 2 | 2 | 3 | 1 | 9 | 3 | 16 | 6 | 23 | 3 | 4 | 10 |
Vac/Vac Membrane | 6 | 10 | 3 | 1 | 3 | 1 | 5 | 2 | – | 1 | 0 | 0 | 1 | 2 | 1 | 7 | 6 | 1 | 17 | 11 | 22 | 5 | 11 | 14 |
Unique Cell Count | 576 | 293 | 326 | 247 | 313 | 416 | 477 | 567 | 467 | 374 | 506 | 42 | 43 | 66 | 390 | 297 | 120 | 644 | 320 | 419 | 75 | 141 | 184 | |
Labelled Cell Count | 642 | 377 | 376 | 291 | 359 | 566 | 668 | 762 | 701 | 564 | 727 | 46 | 48 | 73 | 452 | 352 | 144 | 644 | 320 | 419 | 75 | 141 | 184 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 6.0 | 6.1 | 5.4 | 5.6 | 4.6 | 4.5 | 4.9 | – | 4.4 | 4.4 | 4.5 | 6.9 | 6.9 | 7.6 | 7.4 | 8.3 | 8.9 | 7.2 | 7.3 | 7.7 |
Std Deviation (1e-4) | 1.3 | 1.3 | 1.2 | 1.3 | 1.6 | 1.3 | 1.1 | 1.2 | – | 1.2 | 1.1 | 1.2 | 1.3 | 1.3 | 2.3 | 1.6 | 2.0 | 2.4 | 2.0 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.12 | -0.39 | -0.44 | -0.3 | – | -0.47 | -0.47 | -0.44 | 0.2 | 0.2 | 0.33 | 0.3 | 0.46 | 0.56 | 0.26 | 0.28 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | 2.2 | -0.4 | -2.0 | -2.0 | – | -1.5 | -0.5 | -2.7 | 0 | 0 | 0 | 3.8 | 3.8 | 3.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.3 | 2.8 | 0 | 0 | 8.8 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | -0.6 | 0 |
Nucleus | -0.3 | -3.7 | -4.8 | -8.1 | -7.4 | – | -7.5 | -6.9 | -7.6 | 0.6 | 0.4 | -1.0 | 1.1 | 0.4 | -0.3 |
Nuclear Periphery | -1.4 | -0.3 | 0 | 0 | -2.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -1.1 | -0.7 |
Nucleolus | 1.1 | 2.8 | 0 | 0 | 8.9 | – | 0 | 0 | 0 | -1.7 | -1.8 | -1.6 | -1.8 | -0.2 | 0.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -2.6 | -2.8 | 0 | 0 | -3.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | -4.2 | -1.4 | -1.2 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.7967 | 3.2385 | 3.4027 | 3.5453 | 3.0427 | 3.2192 | 7.26 | 5.756 | 5.9265 | 6.1022 | 5.9249 | 5.8141 | 5.982 | 5.5006 | 5.7397 | 6.234 | 5.7117 | 5.7265 |
Actin | 0.0147 | 0.0008 | 0.0013 | 0.0001 | 0.0017 | 0.0008 | 0.0433 | 0.0001 | 0.0058 | 0.0005 | 0.025 | 0.0047 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 |
Bud | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0003 | 0.0008 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 |
Bud Neck | 0.0008 | 0.0003 | 0.0006 | 0.0001 | 0.001 | 0.0013 | 0.0043 | 0.0001 | 0.0002 | 0.001 | 0.0007 | 0.0009 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0007 | 0.0007 |
Bud Periphery | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.0019 | 0 | 0.0005 | 0.0041 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0006 | 0.0018 | 0.0011 | 0 | 0.0003 | 0.0002 | 0.0023 | 0.0001 | 0.0012 | 0.0005 | 0.0014 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0043 | 0.0007 | 0.001 | 0.0001 | 0.0023 | 0.0006 | 0.0078 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0 |
Cytoplasmic Foci | 0.0054 | 0.0006 | 0.0071 | 0 | 0.0075 | 0.0001 | 0.0095 | 0 | 0.0005 | 0.0001 | 0.0439 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0048 | 0.0006 | 0.0001 | 0.0001 | 0.0024 | 0.0013 | 0.0091 | 0 | 0.0002 | 0.0002 | 0.0008 | 0.0011 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endosome | 0.017 | 0.0005 | 0.0044 | 0.0001 | 0.0223 | 0.0013 | 0.0205 | 0 | 0.0019 | 0.0005 | 0.0093 | 0.0016 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 |
Golgi | 0.0018 | 0.0002 | 0.0036 | 0 | 0.004 | 0.0001 | 0.0063 | 0 | 0.0027 | 0.0001 | 0.0101 | 0.0005 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0016 | 0.0014 | 0.004 | 0 | 0.0306 | 0.0003 | 0.0062 | 0 | 0.0004 | 0.0001 | 0.0122 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 |
Mitochondria | 0.0055 | 0.0011 | 0.0008 | 0.0003 | 0.0018 | 0.0026 | 0.0064 | 0.0002 | 0.0027 | 0.0275 | 0.0035 | 0.0013 | 0.0006 | 0.0005 | 0.0005 | 0.0002 | 0.0005 | 0.0001 |
None | 0.0032 | 0.0003 | 0.0001 | 0 | 0.0011 | 0.0003 | 0.0141 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 |
Nuclear Periphery | 0.0709 | 0.0211 | 0.0219 | 0.0476 | 0.0263 | 0.0142 | 0.0574 | 0.0087 | 0.0163 | 0.0049 | 0.0055 | 0.0074 | 0.0067 | 0.0137 | 0.016 | 0.0019 | 0.024 | 0.0029 |
Nucleolus | 0.0525 | 0.0732 | 0.0768 | 0.0171 | 0.2346 | 0.1452 | 0.0648 | 0.0435 | 0.0653 | 0.0933 | 0.1955 | 0.0853 | 0.0648 | 0.0719 | 0.1028 | 0.0726 | 0.1857 | 0.1078 |
Nucleus | 0.781 | 0.8822 | 0.8598 | 0.932 | 0.611 | 0.8206 | 0.6916 | 0.9265 | 0.8704 | 0.8386 | 0.6285 | 0.8912 | 0.9262 | 0.8936 | 0.8639 | 0.9069 | 0.7008 | 0.8801 |
Peroxisomes | 0.0003 | 0.0006 | 0.0067 | 0 | 0.0046 | 0.0001 | 0.0034 | 0 | 0.0002 | 0.0002 | 0.0278 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0242 | 0.013 | 0.0095 | 0.0016 | 0.0438 | 0.008 | 0.0439 | 0.0202 | 0.0246 | 0.0155 | 0.0332 | 0.0038 | 0.001 | 0.0174 | 0.0152 | 0.0174 | 0.084 | 0.0079 |
Vacuole | 0.0045 | 0.0007 | 0.0003 | 0.0001 | 0.0022 | 0.0012 | 0.0028 | 0.0001 | 0.0035 | 0.006 | 0.0003 | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0002 | 0.0001 |
Vacuole Periphery | 0.005 | 0.0007 | 0.0007 | 0.0008 | 0.0018 | 0.0011 | 0.0026 | 0.0001 | 0.0024 | 0.0049 | 0.0005 | 0.0004 | 0.0002 | 0.0005 | 0.0004 | 0 | 0.0004 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.7765 | 14.2161 | 7.8441 | 11.1367 | 12.6884 | 13.4487 | 18.7044 | 15.6715 | 11.6862 | 18.9255 |
Translational Efficiency | 0.6602 | 0.9283 | 0.8225 | 0.5704 | 0.7809 | 0.9408 | 0.7296 | 0.5705 | 0.732 | 0.6248 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |