Standard name
Human Ortholog
Description Glycosylated integral membrane protein localized to plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles; VID22 has a paralog, ENV11, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0.06 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0 0 0 – 0 0 0 0.17 0.16 0.23 0.11 0.12 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.2 0 0.12 0.08 0.31 0.37 0.26 – 0.39 0.5 0.39 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nucleus 0.79 0.79 0.71 0.7 0.58 0.54 0.42 0.46 – 0.45 0.45 0.44 0.76 0.74 0.65 0.75 0.73 0.7 0.57 0.67 0.4 0.38 0.46 0.41
Nuclear Periphery 0 0 0.07 0 0.06 0.05 0 0 – 0 0.05 0.05 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Nucleolus 0.25 0.2 0.21 0.25 0.3 0.4 0.51 0.51 – 0.54 0.41 0.51 0.1 0.09 0.12 0.16 0.2 0.22 0.31 0.2 0.34 0.35 0.29 0.29
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 – 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0.06 0.05 0 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.07 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 1 0 3 7 – 2 4 1 0 0 0 1 0 0 3 3 1 1 0 2
Bud Neck 0 0 2 1 0 0 2 5 – 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Bud Site 0 0 0 0 1 10 14 9 – 26 18 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 12 11 24 13 7 9 – 9 11 5 7 7 15 44 35 14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 – 0 0 0 0 0 1 5 5 3 0 0 1 0 0 1
Endosome 4 0 8 1 8 1 0 1 – 0 0 0 0 0 0 0 0 1 14 6 18 3 8 15
Golgi 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 5 0 4 0 0 1
Mitochondria 3 59 9 29 24 128 175 147 – 181 186 199 0 0 0 18 6 6 3 4 6 1 5 2
Nucleus 457 232 233 174 180 226 202 261 – 208 170 225 32 32 43 293 217 84 368 214 167 28 65 74
Nuclear Periphery 17 10 22 10 19 19 12 20 – 19 20 27 0 1 1 22 14 6 10 3 17 0 0 4
Nucleolus 143 60 68 61 95 166 244 291 – 251 153 256 4 4 8 61 60 26 198 63 142 26 40 53
Peroxisomes 2 0 0 0 0 0 0 1 – 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0
SpindlePole 6 1 17 3 4 2 4 9 – 4 2 4 2 2 3 1 9 3 16 6 23 3 4 10
Vac/Vac Membrane 6 10 3 1 3 1 5 2 – 1 0 0 1 2 1 7 6 1 17 11 22 5 11 14
Unique Cell Count 576 293 326 247 313 416 477 567 467 374 506 42 43 66 390 297 120 644 320 419 75 141 184
Labelled Cell Count 642 377 376 291 359 566 668 762 701 564 727 46 48 73 452 352 144 644 320 419 75 141 184


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 6.0 6.1 5.4 5.6 4.6 4.5 4.9 – 4.4 4.4 4.5 6.9 6.9 7.6 7.4 8.3 8.9 7.2 7.3 7.7
Std Deviation (1e-4) 1.3 1.3 1.2 1.3 1.6 1.3 1.1 1.2 – 1.2 1.1 1.2 1.3 1.3 2.3 1.6 2.0 2.4 2.0 1.5 1.6
Intensity Change (Log2) – – – -0.16 -0.12 -0.39 -0.44 -0.3 – -0.47 -0.47 -0.44 0.2 0.2 0.33 0.3 0.46 0.56 0.26 0.28 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 2.2 -0.4 -2.0 -2.0 – -1.5 -0.5 -2.7 0 0 0 3.8 3.8 3.2
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0.1 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 4.3 2.8 0 0 8.8 – 0 0 0 0 0 0 1.3 -0.6 0
Nucleus -0.3 -3.7 -4.8 -8.1 -7.4 – -7.5 -6.9 -7.6 0.6 0.4 -1.0 1.1 0.4 -0.3
Nuclear Periphery -1.4 -0.3 0 0 -2.2 – 0 0 0 0 0 0 -0.6 -1.1 -0.7
Nucleolus 1.1 2.8 0 0 8.9 – 0 0 0 -1.7 -1.8 -1.6 -1.8 -0.2 0.2
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole -2.6 -2.8 0 0 -3.1 – 0 0 0 0 0 0 -4.2 -1.4 -1.2
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7967 3.2385 3.4027 3.5453 3.0427 3.2192 7.26 5.756 5.9265 6.1022 5.9249 5.8141 5.982 5.5006 5.7397 6.234 5.7117 5.7265
Actin 0.0147 0.0008 0.0013 0.0001 0.0017 0.0008 0.0433 0.0001 0.0058 0.0005 0.025 0.0047 0.0001 0.0001 0.0001 0.0001 0.0004 0
Bud 0.0004 0.0001 0.0001 0 0.0001 0.0001 0.0009 0 0.0003 0.0008 0.0003 0.0001 0 0.0001 0.0001 0 0.0002 0
Bud Neck 0.0008 0.0003 0.0006 0.0001 0.001 0.0013 0.0043 0.0001 0.0002 0.001 0.0007 0.0009 0.0001 0.0003 0.0002 0.0002 0.0007 0.0007
Bud Periphery 0.0011 0.0001 0.0001 0 0.0003 0.0004 0.0019 0 0.0005 0.0041 0.0006 0.0004 0.0001 0.0002 0.0001 0 0.0001 0.0001
Bud Site 0.0006 0.0018 0.0011 0 0.0003 0.0002 0.0023 0.0001 0.0012 0.0005 0.0014 0.0001 0 0.0004 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0002 0 0 0 0.0001 0.0001 0.0004 0 0.0002 0.0009 0.0001 0.0001 0 0.0001 0 0 0 0
Cytoplasm 0.0043 0.0007 0.001 0.0001 0.0023 0.0006 0.0078 0.0002 0.0004 0.0001 0.0003 0.0002 0 0.0002 0.0001 0.0001 0.0009 0
Cytoplasmic Foci 0.0054 0.0006 0.0071 0 0.0075 0.0001 0.0095 0 0.0005 0.0001 0.0439 0.0001 0 0 0 0.0001 0.0002 0
Eisosomes 0.0001 0 0 0 0 0 0.0004 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0048 0.0006 0.0001 0.0001 0.0024 0.0013 0.0091 0 0.0002 0.0002 0.0008 0.0011 0 0 0 0 0.0001 0
Endosome 0.017 0.0005 0.0044 0.0001 0.0223 0.0013 0.0205 0 0.0019 0.0005 0.0093 0.0016 0 0.0001 0.0001 0.0001 0.0005 0
Golgi 0.0018 0.0002 0.0036 0 0.004 0.0001 0.0063 0 0.0027 0.0001 0.0101 0.0005 0 0 0 0 0.0001 0
Lipid Particles 0.0016 0.0014 0.004 0 0.0306 0.0003 0.0062 0 0.0004 0.0001 0.0122 0.0002 0 0.0001 0.0001 0 0.0004 0
Mitochondria 0.0055 0.0011 0.0008 0.0003 0.0018 0.0026 0.0064 0.0002 0.0027 0.0275 0.0035 0.0013 0.0006 0.0005 0.0005 0.0002 0.0005 0.0001
None 0.0032 0.0003 0.0001 0 0.0011 0.0003 0.0141 0.0001 0.0002 0.0001 0.0004 0.0001 0 0.0001 0.0001 0.0001 0.0004 0
Nuclear Periphery 0.0709 0.0211 0.0219 0.0476 0.0263 0.0142 0.0574 0.0087 0.0163 0.0049 0.0055 0.0074 0.0067 0.0137 0.016 0.0019 0.024 0.0029
Nucleolus 0.0525 0.0732 0.0768 0.0171 0.2346 0.1452 0.0648 0.0435 0.0653 0.0933 0.1955 0.0853 0.0648 0.0719 0.1028 0.0726 0.1857 0.1078
Nucleus 0.781 0.8822 0.8598 0.932 0.611 0.8206 0.6916 0.9265 0.8704 0.8386 0.6285 0.8912 0.9262 0.8936 0.8639 0.9069 0.7008 0.8801
Peroxisomes 0.0003 0.0006 0.0067 0 0.0046 0.0001 0.0034 0 0.0002 0.0002 0.0278 0.0001 0 0 0 0 0.0001 0
Punctate Nuclear 0.0242 0.013 0.0095 0.0016 0.0438 0.008 0.0439 0.0202 0.0246 0.0155 0.0332 0.0038 0.001 0.0174 0.0152 0.0174 0.084 0.0079
Vacuole 0.0045 0.0007 0.0003 0.0001 0.0022 0.0012 0.0028 0.0001 0.0035 0.006 0.0003 0.0004 0.0001 0.0007 0.0001 0 0.0002 0.0001
Vacuole Periphery 0.005 0.0007 0.0007 0.0008 0.0018 0.0011 0.0026 0.0001 0.0024 0.0049 0.0005 0.0004 0.0002 0.0005 0.0004 0 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.7765 14.2161 7.8441 11.1367 12.6884 13.4487 18.7044 15.6715 11.6862 18.9255
Translational Efficiency 0.6602 0.9283 0.8225 0.5704 0.7809 0.9408 0.7296 0.5705 0.732 0.6248

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘