Standard name
Human Ortholog
Description Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; homologous to S. pombe rad18
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.05 – 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.09 0.11 0.09 0.11 0.09 0.09 0.11 0.09 – 0.06 0.06 0.2 0.27 0.33 0.1 0.09 0.18 0 0 0 0.05 0 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.13 0.07 0 0 0.09 0 0 0 0 – 0 0 0.07 0.06 0 0.07 0.06 0 0.1 0 0.08 0.09 0.15 0.1
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0.44 0.59 0.39 0.62 0.63 0.63 0.77 – 0.83 0.79 0.1 0.09 0.31 0.11 0.06 0.09 0 0 0.06 0.06 0.06 0.08
Nucleus 0.66 0.6 0.46 0.43 0.42 0.4 0.42 0.44 0.4 – 0.38 0.36 0.55 0.48 0.31 0.64 0.69 0.58 0.47 0.49 0.31 0.32 0.22 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0.08 0.1 0.08 0.08 0.06 – 0.08 0.09 0.06 0 0 0 0.08 0.11 0.09 0.1 0.08 0.11 0.07 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0.09 0.07 0.12 0.08 0 0.05 0 – 0 0 0 0 0 0 0 0.05 0.11 0.09 0.13 0.13 0.14 0.13
Vac/Vac Membrane 0.09 0.21 0 0 0.05 0 0 0 0 – 0 0 0.11 0.09 0.14 0.13 0.1 0.07 0.07 0.16 0.19 0.17 0.23 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 2 2 12 5 5 10 10 – 14 20 0 0 0 0 1 1 0 7 2 3 2 6
Bud Neck 0 0 3 1 3 0 0 4 1 – 2 0 0 0 0 0 0 0 0 0 1 1 2 4
Bud Site 0 1 1 8 7 8 15 14 13 – 14 16 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 26 27 21 33 25 29 45 26 – 21 20 43 53 79 10 8 10 2 10 8 9 9 16
Endoplasmic Reticulum 0 0 2 2 0 1 0 0 0 – 0 0 0 2 2 4 3 3 0 0 1 0 2 4
Endosome 10 20 10 2 27 9 5 9 1 – 1 2 14 12 4 7 5 2 10 15 28 18 47 27
Golgi 0 0 2 0 1 0 1 0 0 – 0 0 1 0 1 1 0 0 1 0 1 0 2 1
Mitochondria 1 43 113 131 111 181 204 268 222 – 276 280 21 17 75 11 5 5 3 13 20 12 18 23
Nucleus 50 175 119 96 122 116 134 185 115 – 128 128 118 94 74 65 59 32 52 185 104 69 72 43
Nuclear Periphery 2 3 1 0 1 1 2 1 3 – 2 5 1 3 4 5 2 0 4 7 13 1 4 5
Nucleolus 3 10 5 15 24 30 26 36 16 – 28 32 12 4 6 4 7 6 10 36 28 24 22 16
Peroxisomes 0 0 0 0 0 1 1 0 1 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 5 7 22 15 35 23 13 21 8 – 12 12 5 8 4 1 3 3 11 36 44 28 44 35
Vac/Vac Membrane 7 61 9 4 13 10 14 19 4 – 8 14 24 18 33 13 9 4 7 59 63 35 76 78
Unique Cell Count 76 293 257 223 288 291 322 425 288 333 354 214 196 241 101 86 55 110 383 334 214 326 277
Labelled Cell Count 82 346 316 297 389 410 449 612 420 506 529 239 212 283 121 103 66 110 383 334 214 326 277


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.4 3.8 3.7 4.1 3.7 3.7 3.7 3.1 – 3.1 3.0 4.6 4.6 3.9 6.9 7.4 7.6 4.6 4.7 5.3
Std Deviation (1e-4) 1.3 0.7 0.9 1.1 1.4 1.4 1.6 1.4 0.9 – 1.1 1.1 1.1 1.1 0.9 1.6 2.0 2.3 1.2 1.5 1.7
Intensity Change (Log2) – – – -0.04 0.09 -0.04 -0.04 -0.05 -0.28 – -0.3 -0.37 0.27 0.27 0.04 0.86 0.96 0.98 0.28 0.29 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 3.3 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.607 0.9691 1.023 0.4159 1.0984 1.2964 -0.1833 -0.4664 -0.1874 -0.8513 -0.4629 -0.4092 3.4876 3.7254 3.171 3.8078 3.4493 3.4275
Actin 0.0802 0.0004 0.0214 0.0007 0.0092 0.0091 0.0123 0.0001 0.0006 0.0001 0.0023 0.0011 0.0152 0.0002 0.0008 0.0014 0.0003 0.0001
Bud 0.0007 0.0007 0.0002 0.0007 0.0004 0.0002 0.0006 0.0012 0.0002 0.0001 0.0002 0.0002 0.0003 0.0004 0.0003 0.0001 0.0001 0.0001
Bud Neck 0.0046 0.0012 0.0021 0.0025 0.0037 0.0076 0.0022 0.0008 0.0015 0.0012 0.0017 0.005 0.0032 0.0006 0.001 0.0017 0.0012 0.0063
Bud Periphery 0.0014 0.001 0.0002 0.0008 0.0008 0.0006 0.0012 0.0005 0.0002 0.0001 0.0003 0.0007 0.0002 0.001 0.0002 0.0002 0.0001 0.0001
Bud Site 0.003 0.003 0.0022 0.0009 0.0021 0.0006 0.0016 0.0039 0.0034 0.0001 0.0003 0.0003 0.0085 0.0011 0.0022 0.0011 0.0003 0.0003
Cell Periphery 0.0003 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.0189 0.0512 0.0122 0.0311 0.0338 0.0193 0.0147 0.0241 0.0046 0.0062 0.0069 0.0043 0.0101 0.0263 0.0309 0.0275 0.0219 0.0094
Cytoplasmic Foci 0.0149 0.0056 0.0032 0.0016 0.0077 0.0015 0.0068 0.0027 0.0015 0.0008 0.0009 0.0018 0.0116 0.0051 0.0012 0.0047 0.0171 0.0004
Eisosomes 0.001 0 0.0002 0 0.0002 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0069 0.0034 0.0009 0.0025 0.0038 0.0036 0.004 0.0011 0.0003 0.0004 0.0036 0.0008 0.0008 0.0027 0.001 0.002 0.001 0.0005
Endosome 0.02 0.0061 0.0036 0.0024 0.0189 0.0091 0.0159 0.0017 0.0014 0.0003 0.0047 0.0015 0.0035 0.0031 0.0029 0.0074 0.0107 0.0006
Golgi 0.0113 0.0002 0.0011 0.0002 0.0041 0.0047 0.0048 0 0.0004 0 0.0009 0.0003 0.0017 0.0001 0.0001 0.0006 0.0008 0
Lipid Particles 0.0199 0.0018 0.0016 0.0002 0.0094 0.0058 0.0062 0.0002 0.0006 0.0001 0.001 0.0009 0.0063 0.0005 0.0006 0.0035 0.0375 0.0001
Mitochondria 0.0067 0.0007 0.0007 0.0005 0.0077 0.0072 0.0048 0.0003 0.0004 0.0002 0.0348 0.0055 0.0006 0.0067 0.0004 0.0009 0.0007 0.0003
None 0.0642 0.054 0.0164 0.0425 0.0543 0.0317 0.0064 0.0196 0.0029 0.0337 0.0013 0.0104 0.0289 0.0797 0.0348 0.1529 0.0496 0.036
Nuclear Periphery 0.0465 0.0241 0.0202 0.0313 0.0691 0.0336 0.0498 0.0281 0.0179 0.0148 0.0553 0.0285 0.0062 0.0119 0.0254 0.0169 0.0064 0.0062
Nucleolus 0.0191 0.0243 0.0515 0.0616 0.0316 0.065 0.0205 0.0281 0.0262 0.0396 0.0399 0.0526 0.039 0.0262 0.0315 0.0551 0.1078 0.0877
Nucleus 0.6114 0.746 0.8201 0.7376 0.5197 0.7091 0.7506 0.8141 0.8644 0.7853 0.6675 0.7907 0.8122 0.7539 0.8284 0.6056 0.7134 0.806
Peroxisomes 0.0117 0.0003 0.0008 0.0002 0.002 0.0011 0.0019 0 0.0002 0 0.0003 0.0001 0.0025 0.0002 0.0002 0.0016 0.0044 0
Punctate Nuclear 0.0528 0.0702 0.039 0.0802 0.217 0.0866 0.0897 0.0715 0.0721 0.1169 0.1755 0.0925 0.0481 0.0753 0.036 0.1123 0.0248 0.045
Vacuole 0.0028 0.0048 0.0019 0.0018 0.0025 0.0019 0.0031 0.0014 0.0008 0.0001 0.0012 0.0013 0.0008 0.0028 0.0015 0.0032 0.0015 0.0007
Vacuole Periphery 0.0015 0.0007 0.0004 0.0006 0.0018 0.0016 0.0025 0.0004 0.0003 0.0001 0.0012 0.0013 0.0002 0.0022 0.0004 0.0008 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.8529 14.1543 3.501 7.7201 19.2459 22.7482 11.628 7.4456 8.2983 23.0441
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
151 90 1398 857 1457 1700 1086 76 1608 1790 2484 933

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 546.00 782.51 888.85 810.21 644.38 718.18 883.86 899.45 635.14 721.41 886.67 817.48
Standard Deviation 92.54 105.57 125.81 129.15 84.03 94.78 121.67 146.75 89.58 96.38 124.04 132.94
Intensity Change Log 2 — 0.519208 0.703039 0.569395 — 0.156434 0.455906 0.481131 — 0.334204 0.574534 0.522287

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000142 0.001045 0.000749 0.000979 0.000239 0.000477 0.000894 0.001022 0.000230 0.000506 0.000812 0.000983
Bud Neck 0.007375 0.015029 0.014995 0.017281 0.005977 0.011285 0.009336 0.002993 0.006108 0.011473 0.012521 0.016117
Bud Site 0.002409 0.004450 0.002779 0.029575 0.001078 0.006969 0.005055 0.002522 0.001203 0.006842 0.003774 0.027372
Cell Periphery 0.000333 0.000141 0.000091 0.000360 0.000117 0.000157 0.000124 0.000144 0.000137 0.000157 0.000105 0.000342
Cytoplasm 0.261633* 0.067376 0.025211 0.187617* 0.150188* 0.140818* 0.027124 0.069987 0.160653* 0.137126* 0.026047 0.178035*
Cytoplasmic Foci 0.128753 0.051109 0.001011 0.026632 0.029388 0.036637 0.000775 0.009791 0.038719 0.037365 0.000908 0.025260
Eisosomes 0.000137 0.000108 0.000026 0.000059 0.000045 0.000061 0.000035 0.000079 0.000054 0.000064 0.000030 0.000060
Endoplasmic Reticulum 0.002230 0.006388 0.004942 0.004701 0.001782 0.002564 0.007133 0.012551 0.001824 0.002756 0.005900 0.005341
Endosome 0.004466 0.003344 0.000781 0.020693 0.001749 0.006136 0.001833 0.007152 0.002004 0.005995 0.001241 0.019590
Golgi 0.000845 0.004847 0.000469 0.012409 0.000444 0.001735 0.000872 0.012536 0.000482 0.001891 0.000645 0.012419
Lipid Particles 0.022561 0.007385 0.000384 0.002413 0.008152 0.010694 0.000544 0.002480 0.009505 0.010528 0.000454 0.002418
Mitochondria 0.004541 0.009065 0.002053 0.017366 0.004989 0.013983 0.002145 0.045547 0.004947 0.013736 0.002093 0.019662
Mitotic Spindle 0.002750 0.052726 0.023542 0.114375* 0.006420 0.024798 0.014440 0.084370 0.006076 0.026202 0.019563 0.111931*
None 0.041696 0.010664 0.007947 0.022491 0.034943 0.020007 0.010704 0.029437 0.035577 0.019537 0.009152 0.023057
Nuclear Periphery 0.002193 0.002710 0.002068 0.002710 0.004677 0.005772 0.001698 0.003554 0.004444 0.005618 0.001907 0.002779
Nuclear Periphery Foci 0.003553 0.015297 0.001021 0.002306 0.006272 0.004385 0.001016 0.026492 0.006017 0.004934 0.001019 0.004276
Nucleolus 0.020543 0.043685 0.025506 0.011615 0.049445 0.039752 0.013448 0.030709 0.046731 0.039950 0.020234 0.013170
Nucleus 0.475313* 0.678034* 0.882888* 0.479212* 0.688505* 0.654367* 0.896068* 0.540301* 0.668485* 0.655557* 0.888650* 0.484188*
Peroxisomes 0.003341 0.015961 0.000441 0.003243 0.002583 0.006191 0.000433 0.025215 0.002654 0.006683 0.000438 0.005033
Vacuole 0.014130 0.009886 0.002752 0.038837 0.002701 0.011413 0.006021 0.041091 0.003775 0.011336 0.004181 0.039021
Vacuole Periphery 0.001056 0.000750 0.000344 0.005126 0.000305 0.001798 0.000303 0.052027 0.000376 0.001745 0.000326 0.008947

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.27 -14.12 -9.94 -0.94 -2.97 -1.38 -8.67 -6.69 -5.36 -1.74 -1.79 -9.77 -8.70 -6.08 -2.53
Bud Neck -1.70 -8.15 -5.52 -1.48 2.87 -3.82 -8.69 -1.71 1.38 5.48 -4.11 -14.56 -8.58 -5.30 1.66
Bud Site -1.99 -2.23 -6.56 -4.49 -5.97 -4.40 -4.69 -4.34 0.66 0.43 -4.48 -7.37 -7.68 -4.71 -5.67
Cell Periphery 1.63 2.10 0.82 -3.23 -6.46 -2.55 -0.96 -2.12 -0.49 -1.48 -0.91 1.91 -4.04 -3.52 -5.96
Cytoplasm 8.33 12.30 6.79 -3.62 -13.21 1.39 20.10 4.72 4.21 -2.28 3.33 24.18 5.63 3.06 -13.24
Cytoplasmic Foci 4.75 9.40 8.63 3.62 -5.96 -1.77 14.19 8.96 10.98 -1.87 0.90 16.33 10.95 11.22 -6.22
Eisosomes 0.08 6.95 3.70 2.71 -10.50 -4.21 0.54 -3.96 -3.25 -4.04 -2.62 5.78 -5.99 -3.85 -10.40
Endoplasmic Reticulum -2.86 -3.99 -7.37 -2.23 -6.31 -2.22 -14.65 -6.75 -6.48 -3.60 -2.84 -16.83 -14.75 -13.62 -5.74
Endosome -0.34 4.08 -2.48 -1.90 -5.24 -6.36 1.10 -2.10 1.32 -2.41 -6.16 3.98 -4.49 -1.61 -5.22
Golgi -1.18 0.90 -2.98 -0.05 -3.42 -3.68 -0.18 -1.35 -0.88 -1.32 -3.80 0.19 -3.58 -2.19 -3.58
Lipid Particles 2.42 4.23 3.89 3.36 -6.67 -2.19 9.39 5.94 8.84 -3.61 -1.03 10.35 8.37 11.33 -6.93
Mitochondria -1.12 1.31 -1.60 -0.01 -2.87 -5.66 3.15 -1.10 -0.18 -1.39 -5.83 3.35 -1.69 2.28 -3.21
Mitotic Spindle -3.14 -7.33 -11.52 -1.76 -6.66 -5.06 -5.49 -2.81 -2.10 -1.75 -5.85 -9.58 -12.34 -9.44 -7.79
None 4.05 4.69 3.93 -0.72 -3.15 5.25 9.54 2.26 0.43 -0.94 5.92 11.22 8.11 2.92 -3.09
Nuclear Periphery -1.08 -1.41 -3.65 -1.55 -4.22 -1.62 3.62 -1.01 -0.02 -3.09 -1.91 2.77 -1.40 0.79 -5.93
Nuclear Periphery Foci -2.04 1.59 0.26 2.11 -2.46 1.95 3.68 -1.17 -1.45 -1.69 1.10 4.18 1.15 0.20 -2.88
Nucleolus -2.22 -2.71 0.84 3.12 6.99 2.64 9.22 3.16 1.98 -0.73 1.96 5.11 8.55 7.15 4.92
Nucleus -3.26 -12.93 -3.70 1.00 17.30 2.06 -16.93 1.14 0.56 5.68 0.59 -19.34 4.94 4.54 20.12
Peroxisomes -2.11 4.56 2.94 2.58 -2.51 -4.47 5.12 -0.69 -0.11 -1.04 -4.87 6.73 1.45 5.16 -2.16
Vacuole -0.55 2.63 -6.76 -3.59 -12.12 -7.63 -6.09 -3.78 -3.33 -3.30 -6.03 -4.09 -12.92 -11.11 -12.06
Vacuole Periphery 0.38 1.29 -1.52 -2.36 -3.40 -3.64 0.58 -1.40 -1.28 -1.40 -3.51 1.00 -2.68 -1.34 -2.75
Standard name
Human Ortholog
Description Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; homologous to S. pombe rad18
Localization
Cell Percentages nucleus (78%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Smc6

Smc6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smc6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available