Standard name
Description Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; homologous to S. pombe rad18
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.05 – 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.09 0.11 0.09 0.11 0.09 0.09 0.11 0.09 – 0.06 0.06 0.2 0.27 0.33 0.1 0.09 0.18 0 0 0 0.05 0 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.13 0.07 0 0 0.09 0 0 0 0 – 0 0 0.07 0.06 0 0.07 0.06 0 0.1 0 0.08 0.09 0.15 0.1
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0.44 0.59 0.39 0.62 0.63 0.63 0.77 – 0.83 0.79 0.1 0.09 0.31 0.11 0.06 0.09 0 0 0.06 0.06 0.06 0.08
Nucleus 0.66 0.6 0.46 0.43 0.42 0.4 0.42 0.44 0.4 – 0.38 0.36 0.55 0.48 0.31 0.64 0.69 0.58 0.47 0.49 0.31 0.32 0.22 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0.08 0.1 0.08 0.08 0.06 – 0.08 0.09 0.06 0 0 0 0.08 0.11 0.09 0.1 0.08 0.11 0.07 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0.09 0.07 0.12 0.08 0 0.05 0 – 0 0 0 0 0 0 0 0.05 0.11 0.09 0.13 0.13 0.14 0.13
Vac/Vac Membrane 0.09 0.21 0 0 0.05 0 0 0 0 – 0 0 0.11 0.09 0.14 0.13 0.1 0.07 0.07 0.16 0.19 0.17 0.23 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 2 2 12 5 5 10 10 – 14 20 0 0 0 0 1 1 0 7 2 3 2 6
Bud Neck 0 0 3 1 3 0 0 4 1 – 2 0 0 0 0 0 0 0 0 0 1 1 2 4
Bud Site 0 1 1 8 7 8 15 14 13 – 14 16 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 26 27 21 33 25 29 45 26 – 21 20 43 53 79 10 8 10 2 10 8 9 9 16
Endoplasmic Reticulum 0 0 2 2 0 1 0 0 0 – 0 0 0 2 2 4 3 3 0 0 1 0 2 4
Endosome 10 20 10 2 27 9 5 9 1 – 1 2 14 12 4 7 5 2 10 15 28 18 47 27
Golgi 0 0 2 0 1 0 1 0 0 – 0 0 1 0 1 1 0 0 1 0 1 0 2 1
Mitochondria 1 43 113 131 111 181 204 268 222 – 276 280 21 17 75 11 5 5 3 13 20 12 18 23
Nucleus 50 175 119 96 122 116 134 185 115 – 128 128 118 94 74 65 59 32 52 185 104 69 72 43
Nuclear Periphery 2 3 1 0 1 1 2 1 3 – 2 5 1 3 4 5 2 0 4 7 13 1 4 5
Nucleolus 3 10 5 15 24 30 26 36 16 – 28 32 12 4 6 4 7 6 10 36 28 24 22 16
Peroxisomes 0 0 0 0 0 1 1 0 1 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 5 7 22 15 35 23 13 21 8 – 12 12 5 8 4 1 3 3 11 36 44 28 44 35
Vac/Vac Membrane 7 61 9 4 13 10 14 19 4 – 8 14 24 18 33 13 9 4 7 59 63 35 76 78
Unique Cell Count 76 293 257 223 288 291 322 425 288 333 354 214 196 241 101 86 55 110 383 334 214 326 277
Labelled Cell Count 82 346 316 297 389 410 449 612 420 506 529 239 212 283 121 103 66 110 383 334 214 326 277


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.4 3.8 3.7 4.1 3.7 3.7 3.7 3.1 – 3.1 3.0 4.6 4.6 3.9 6.9 7.4 7.6 4.6 4.7 5.3
Std Deviation (1e-4) 1.3 0.7 0.9 1.1 1.4 1.4 1.6 1.4 0.9 – 1.1 1.1 1.1 1.1 0.9 1.6 2.0 2.3 1.2 1.5 1.7
Intensity Change (Log2) – – – -0.04 0.09 -0.04 -0.04 -0.05 -0.28 – -0.3 -0.37 0.27 0.27 0.04 0.86 0.96 0.98 0.28 0.29 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 3.3 – –