Standard name | |
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Human Ortholog | |
Description | Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; homologous to S. pombe rad18 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 2 | 2 | 12 | 5 | 5 | 10 | 10 | – | 14 | 20 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 7 | 2 | 3 | 2 | 6 |
Bud Neck | 0 | 0 | 3 | 1 | 3 | 0 | 0 | 4 | 1 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 4 |
Bud Site | 0 | 1 | 1 | 8 | 7 | 8 | 15 | 14 | 13 | – | 14 | 16 | 0 | 1 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 26 | 27 | 21 | 33 | 25 | 29 | 45 | 26 | – | 21 | 20 | 43 | 53 | 79 | 10 | 8 | 10 | 2 | 10 | 8 | 9 | 9 | 16 |
Endoplasmic Reticulum | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 2 | 4 | 3 | 3 | 0 | 0 | 1 | 0 | 2 | 4 |
Endosome | 10 | 20 | 10 | 2 | 27 | 9 | 5 | 9 | 1 | – | 1 | 2 | 14 | 12 | 4 | 7 | 5 | 2 | 10 | 15 | 28 | 18 | 47 | 27 |
Golgi | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 |
Mitochondria | 1 | 43 | 113 | 131 | 111 | 181 | 204 | 268 | 222 | – | 276 | 280 | 21 | 17 | 75 | 11 | 5 | 5 | 3 | 13 | 20 | 12 | 18 | 23 |
Nucleus | 50 | 175 | 119 | 96 | 122 | 116 | 134 | 185 | 115 | – | 128 | 128 | 118 | 94 | 74 | 65 | 59 | 32 | 52 | 185 | 104 | 69 | 72 | 43 |
Nuclear Periphery | 2 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 3 | – | 2 | 5 | 1 | 3 | 4 | 5 | 2 | 0 | 4 | 7 | 13 | 1 | 4 | 5 |
Nucleolus | 3 | 10 | 5 | 15 | 24 | 30 | 26 | 36 | 16 | – | 28 | 32 | 12 | 4 | 6 | 4 | 7 | 6 | 10 | 36 | 28 | 24 | 22 | 16 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5 | 7 | 22 | 15 | 35 | 23 | 13 | 21 | 8 | – | 12 | 12 | 5 | 8 | 4 | 1 | 3 | 3 | 11 | 36 | 44 | 28 | 44 | 35 |
Vac/Vac Membrane | 7 | 61 | 9 | 4 | 13 | 10 | 14 | 19 | 4 | – | 8 | 14 | 24 | 18 | 33 | 13 | 9 | 4 | 7 | 59 | 63 | 35 | 76 | 78 |
Unique Cell Count | 76 | 293 | 257 | 223 | 288 | 291 | 322 | 425 | 288 | 333 | 354 | 214 | 196 | 241 | 101 | 86 | 55 | 110 | 383 | 334 | 214 | 326 | 277 | |
Labelled Cell Count | 82 | 346 | 316 | 297 | 389 | 410 | 449 | 612 | 420 | 506 | 529 | 239 | 212 | 283 | 121 | 103 | 66 | 110 | 383 | 334 | 214 | 326 | 277 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 4.4 | 3.8 | 3.7 | 4.1 | 3.7 | 3.7 | 3.7 | 3.1 | – | 3.1 | 3.0 | 4.6 | 4.6 | 3.9 | 6.9 | 7.4 | 7.6 | 4.6 | 4.7 | 5.3 |
Std Deviation (1e-4) | 1.3 | 0.7 | 0.9 | 1.1 | 1.4 | 1.4 | 1.6 | 1.4 | 0.9 | – | 1.1 | 1.1 | 1.1 | 1.1 | 0.9 | 1.6 | 2.0 | 2.3 | 1.2 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.09 | -0.04 | -0.04 | -0.05 | -0.28 | – | -0.3 | -0.37 | 0.27 | 0.27 | 0.04 | 0.86 | 0.96 | 0.98 | 0.28 | 0.29 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.3 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.607 | 0.9691 | 1.023 | 0.4159 | 1.0984 | 1.2964 | -0.1833 | -0.4664 | -0.1874 | -0.8513 | -0.4629 | -0.4092 | 3.4876 | 3.7254 | 3.171 | 3.8078 | 3.4493 | 3.4275 |
Actin | 0.0802 | 0.0004 | 0.0214 | 0.0007 | 0.0092 | 0.0091 | 0.0123 | 0.0001 | 0.0006 | 0.0001 | 0.0023 | 0.0011 | 0.0152 | 0.0002 | 0.0008 | 0.0014 | 0.0003 | 0.0001 |
Bud | 0.0007 | 0.0007 | 0.0002 | 0.0007 | 0.0004 | 0.0002 | 0.0006 | 0.0012 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0046 | 0.0012 | 0.0021 | 0.0025 | 0.0037 | 0.0076 | 0.0022 | 0.0008 | 0.0015 | 0.0012 | 0.0017 | 0.005 | 0.0032 | 0.0006 | 0.001 | 0.0017 | 0.0012 | 0.0063 |
Bud Periphery | 0.0014 | 0.001 | 0.0002 | 0.0008 | 0.0008 | 0.0006 | 0.0012 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0007 | 0.0002 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.003 | 0.003 | 0.0022 | 0.0009 | 0.0021 | 0.0006 | 0.0016 | 0.0039 | 0.0034 | 0.0001 | 0.0003 | 0.0003 | 0.0085 | 0.0011 | 0.0022 | 0.0011 | 0.0003 | 0.0003 |
Cell Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0189 | 0.0512 | 0.0122 | 0.0311 | 0.0338 | 0.0193 | 0.0147 | 0.0241 | 0.0046 | 0.0062 | 0.0069 | 0.0043 | 0.0101 | 0.0263 | 0.0309 | 0.0275 | 0.0219 | 0.0094 |
Cytoplasmic Foci | 0.0149 | 0.0056 | 0.0032 | 0.0016 | 0.0077 | 0.0015 | 0.0068 | 0.0027 | 0.0015 | 0.0008 | 0.0009 | 0.0018 | 0.0116 | 0.0051 | 0.0012 | 0.0047 | 0.0171 | 0.0004 |
Eisosomes | 0.001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0069 | 0.0034 | 0.0009 | 0.0025 | 0.0038 | 0.0036 | 0.004 | 0.0011 | 0.0003 | 0.0004 | 0.0036 | 0.0008 | 0.0008 | 0.0027 | 0.001 | 0.002 | 0.001 | 0.0005 |
Endosome | 0.02 | 0.0061 | 0.0036 | 0.0024 | 0.0189 | 0.0091 | 0.0159 | 0.0017 | 0.0014 | 0.0003 | 0.0047 | 0.0015 | 0.0035 | 0.0031 | 0.0029 | 0.0074 | 0.0107 | 0.0006 |
Golgi | 0.0113 | 0.0002 | 0.0011 | 0.0002 | 0.0041 | 0.0047 | 0.0048 | 0 | 0.0004 | 0 | 0.0009 | 0.0003 | 0.0017 | 0.0001 | 0.0001 | 0.0006 | 0.0008 | 0 |
Lipid Particles | 0.0199 | 0.0018 | 0.0016 | 0.0002 | 0.0094 | 0.0058 | 0.0062 | 0.0002 | 0.0006 | 0.0001 | 0.001 | 0.0009 | 0.0063 | 0.0005 | 0.0006 | 0.0035 | 0.0375 | 0.0001 |
Mitochondria | 0.0067 | 0.0007 | 0.0007 | 0.0005 | 0.0077 | 0.0072 | 0.0048 | 0.0003 | 0.0004 | 0.0002 | 0.0348 | 0.0055 | 0.0006 | 0.0067 | 0.0004 | 0.0009 | 0.0007 | 0.0003 |
None | 0.0642 | 0.054 | 0.0164 | 0.0425 | 0.0543 | 0.0317 | 0.0064 | 0.0196 | 0.0029 | 0.0337 | 0.0013 | 0.0104 | 0.0289 | 0.0797 | 0.0348 | 0.1529 | 0.0496 | 0.036 |
Nuclear Periphery | 0.0465 | 0.0241 | 0.0202 | 0.0313 | 0.0691 | 0.0336 | 0.0498 | 0.0281 | 0.0179 | 0.0148 | 0.0553 | 0.0285 | 0.0062 | 0.0119 | 0.0254 | 0.0169 | 0.0064 | 0.0062 |
Nucleolus | 0.0191 | 0.0243 | 0.0515 | 0.0616 | 0.0316 | 0.065 | 0.0205 | 0.0281 | 0.0262 | 0.0396 | 0.0399 | 0.0526 | 0.039 | 0.0262 | 0.0315 | 0.0551 | 0.1078 | 0.0877 |
Nucleus | 0.6114 | 0.746 | 0.8201 | 0.7376 | 0.5197 | 0.7091 | 0.7506 | 0.8141 | 0.8644 | 0.7853 | 0.6675 | 0.7907 | 0.8122 | 0.7539 | 0.8284 | 0.6056 | 0.7134 | 0.806 |
Peroxisomes | 0.0117 | 0.0003 | 0.0008 | 0.0002 | 0.002 | 0.0011 | 0.0019 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0025 | 0.0002 | 0.0002 | 0.0016 | 0.0044 | 0 |
Punctate Nuclear | 0.0528 | 0.0702 | 0.039 | 0.0802 | 0.217 | 0.0866 | 0.0897 | 0.0715 | 0.0721 | 0.1169 | 0.1755 | 0.0925 | 0.0481 | 0.0753 | 0.036 | 0.1123 | 0.0248 | 0.045 |
Vacuole | 0.0028 | 0.0048 | 0.0019 | 0.0018 | 0.0025 | 0.0019 | 0.0031 | 0.0014 | 0.0008 | 0.0001 | 0.0012 | 0.0013 | 0.0008 | 0.0028 | 0.0015 | 0.0032 | 0.0015 | 0.0007 |
Vacuole Periphery | 0.0015 | 0.0007 | 0.0004 | 0.0006 | 0.0018 | 0.0016 | 0.0025 | 0.0004 | 0.0003 | 0.0001 | 0.0012 | 0.0013 | 0.0002 | 0.0022 | 0.0004 | 0.0008 | 0.0003 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.8529 | 14.1543 | 3.501 | 7.7201 | 19.2459 | 22.7482 | 11.628 | 7.4456 | 8.2983 | 23.0441 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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