Standard name
Human Ortholog
Description Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0.1 0.1 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.13 0.11 0.2 0.22 0.22 0.23 0.2 0.21 0.16 0.27 0.1 0.12 0.2 0.14 0.12 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.1 0.05 0 0.05 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.31 0.16 0.38 0.24 0.31 0.2 0.09 0.07 0 0 0.07 0.24 0.34 0.26 0.15 0.27 0.23 0.52 0.39 0.48 0.47 0.46 0.55
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0
Mitochondria 0.09 0.09 0 0.24 0.07 0.36 0.46 0.65 0.65 0.78 0.6 0.16 0 0.05 0.07 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0.06 0.05
Nucleolus 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.54 0.71 0.66 0.38 0.53 0.34 0.24 0.18 0.2 0.16 0.17 0.69 0.72 0.62 0.73 0.76 0.76 0.28 0.5 0.36 0.39 0.38 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 1 2 0 9 0 3 3 4 0 0 1 3 3 1 2 2 1 1 1 2
Bud 1 1 1 3 1 6 15 9 21 22 13 3 3 1 0 0 0 1 1 2 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 0 2 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 27 34 26 17 46 43 64 49 43 34 57 26 40 67 18 25 16 1 0 1 1 2 1
Endoplasmic Reticulum 9 7 4 2 3 9 29 12 8 10 7 2 15 8 1 5 7 3 0 3 2 5 4
Endosome 68 40 88 21 65 40 24 16 2 3 14 59 108 88 19 54 29 127 118 126 113 153 162
Golgi 3 1 2 0 1 0 3 2 0 0 2 0 0 0 3 16 6 7 5 4 2 3 7
Mitochondria 19 24 4 21 14 70 129 161 133 167 124 40 12 17 9 3 5 9 11 11 8 9 11
Nucleus 3 1 1 1 4 3 4 2 7 1 2 0 1 4 3 1 1 1 1 0 1 2 1
Nuclear Periphery 6 6 2 0 1 2 3 7 3 2 1 2 1 1 3 0 0 14 6 10 11 20 15
Nucleolus 11 7 3 5 5 4 1 1 3 1 0 2 10 5 5 9 8 1 0 0 0 1 1
Peroxisomes 0 0 1 0 0 0 0 1 0 0 0 0 3 1 0 0 0 0 1 0 0 1 0
SpindlePole 5 3 10 9 9 2 3 0 2 4 5 1 6 5 0 5 4 3 1 2 1 3 3
Vac/Vac Membrane 117 182 152 33 111 66 67 44 42 35 35 171 231 207 95 154 96 68 151 93 94 126 78
Unique Cell Count 217 258 230 87 210 197 279 246 205 214 208 249 320 333 130 202 126 246 305 261 242 336 297
Labelled Cell Count 269 306 297 113 262 245 352 304 269 285 264 306 430 405 159 275 173 246 305 261 242 336 297


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 7.1 7.5 6.2 7.0 5.9 5.8 5.4 4.9 4.9 5.4 6.3 6.8 7.0 10.8 11.9 12.0 6.2 6.5 6.9
Std Deviation (1e-4) 1.4 1.3 1.6 1.5 1.5 1.4 1.3 1.2 1.0 1.1 1.1 1.0 1.3 1.3 2.5 2.7 2.5 1.3 1.6 1.5
Intensity Change (Log2) -0.28 -0.1 -0.34 -0.37 -0.46 -0.62 -0.61 -0.46 -0.25 -0.13 -0.09 0.53 0.67 0.68 -0.27 -0.21 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 1.4 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 3.2 0 0 4.7 3.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 3.0 2.9 3.4 2.6 2.8 1.4 4.3 -0.3 0.4 2.8 0.7 0.3 0.4
Endoplasmic Reticulum 0 0 1.7 3.9 1.9 0 1.8 1.1 0 1.9 0 0 0 0
Endosome -2.4 -1.6 -4.0 -8.0 -8.4 0 -9.6 -7.8 -3.5 -1.1 -3.0 -4.7 -2.5 -2.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.7 0
Mitochondria 6.6 2.6 9.2 11.4 14.6 0 16.5 13.3 5.4 1.4 2.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1.4 0 0 2.0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.0 0 -2.1 -2.3 0 0 -1.5 -1.1 -2.9 -1.7 -2.1 0 -1.1 0
Vacuole -4.5 -2.8 -6.7 -9.5 -10.7 -9.6 -10.6 -10.4 0.6 1.5 -1.0 1.4 2.3 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1435 4.6287 4.5996 3.7414 4.0252 4.2936 2.5715 2.9904 3.0112 2.54 2.9657 2.5813 2.334 2.1971 2.5192 2.8047 1.9759 2.3294
Actin 0.0134 0.0003 0.0058 0.0001 0.0033 0.0015 0.0122 0.0001 0.0142 0 0.0187 0.0006 0.0125 0.0003 0.0062 0.0232 0 0.0115
Bud 0.0017 0.0044 0.0009 0.0004 0.0026 0.0022 0.0005 0.0009 0.0015 0.0001 0.0045 0.0005 0.0004 0.0004 0.0009 0.0004 0.0001 0.0004
Bud Neck 0.0022 0.0003 0.0012 0.0027 0.0002 0.0015 0.0007 0.0005 0.0005 0.0001 0.0008 0.0005 0.0013 0.0003 0.0037 0.0009 0.0001 0.001
Bud Periphery 0.0017 0.007 0.0016 0.0008 0.0068 0.0058 0.0008 0.0012 0.0018 0.0001 0.0041 0.0014 0.0004 0.0002 0.0008 0.0005 0.0001 0.0008
Bud Site 0.0021 0.0022 0.0077 0.0005 0.0006 0.001 0.0014 0.0015 0.002 0.0001 0.0086 0.0003 0.0018 0.0019 0.0025 0.001 0.0001 0.0006
Cell Periphery 0.0008 0.0003 0.0005 0.0005 0.0001 0.0004 0.0003 0.0004 0.0002 0.0001 0.0003 0.0002 0.0003 0.0001 0.0003 0.0001 0 0.0002
Cytoplasm 0.0185 0.0475 0.0195 0.0047 0.0078 0.0172 0.0079 0.0292 0.0305 0.0037 0.0078 0.0061 0.014 0.0232 0.0245 0.0065 0.0028 0.0144
Cytoplasmic Foci 0.0438 0.0289 0.0361 0.0237 0.0273 0.0323 0.0442 0.0366 0.0391 0.0087 0.045 0.0311 0.0483 0.0232 0.0466 0.0164 0.0132 0.0249
Eisosomes 0.0001 0 0.0002 0 0 0 0.0001 0 0 0 0.0001 0 0.0004 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.004 0.0041 0.0059 0.0019 0.0009 0.0055 0.0062 0.0046 0.0031 0.0033 0.0023 0.0033 0.0046 0.0046 0.0023 0.0012 0.001 0.0038
Endosome 0.534 0.5797 0.5424 0.5444 0.6175 0.5546 0.5691 0.5662 0.5847 0.5795 0.6095 0.6102 0.4897 0.5547 0.5414 0.5967 0.6267 0.5326
Golgi 0.0276 0.0208 0.0403 0.0186 0.0559 0.0323 0.0392 0.027 0.0417 0.0108 0.0858 0.0283 0.0217 0.0135 0.0335 0.0256 0.0236 0.0282
Lipid Particles 0.0432 0.0072 0.0282 0.0057 0.0483 0.006 0.0383 0.0087 0.0103 0.0024 0.0145 0.014 0.0234 0.0049 0.0172 0.0047 0.0012 0.0081
Mitochondria 0.023 0.0101 0.0386 0.0124 0.0195 0.0344 0.0164 0.0075 0.025 0.004 0.0363 0.0176 0.0133 0.0019 0.0126 0.006 0.0013 0.007
None 0.0095 0.0118 0.0197 0.0006 0.0002 0.003 0.0028 0.0012 0.0005 0.0002 0.0003 0.0003 0.0222 0.0015 0.0063 0.0003 0.0001 0.0007
Nuclear Periphery 0.0152 0.0118 0.0089 0.0048 0.0024 0.0104 0.0174 0.0087 0.0041 0.0131 0.0025 0.0067 0.0121 0.0107 0.01 0.0042 0.0023 0.0118
Nucleolus 0.0035 0.0006 0.0009 0.0005 0.0001 0.0007 0.0007 0.0007 0.001 0.0001 0.0003 0.0012 0.003 0.0003 0.0018 0.0004 0 0.001
Nucleus 0.0046 0.0021 0.0031 0.0008 0.0006 0.0025 0.0018 0.0012 0.0013 0.0006 0.0009 0.0012 0.0026 0.0013 0.0021 0.0006 0.0002 0.002
Peroxisomes 0.011 0.001 0.0049 0.0007 0.0075 0.0021 0.0178 0.0011 0.003 0.0001 0.0074 0.0016 0.0129 0.0012 0.0065 0.0045 0.0002 0.0011
Punctate Nuclear 0.0016 0.0003 0.0011 0.0001 0.0001 0.0006 0.003 0.0002 0.0045 0 0.0007 0.0004 0.0042 0.0002 0.0026 0.0011 0 0.0028
Vacuole 0.1718 0.1923 0.1668 0.2418 0.1382 0.1823 0.1592 0.2102 0.1593 0.2167 0.0979 0.1795 0.2225 0.2515 0.1907 0.1786 0.1776 0.2185
Vacuole Periphery 0.0664 0.0674 0.0656 0.1344 0.06 0.104 0.0601 0.0921 0.0717 0.1564 0.0516 0.0951 0.0886 0.1042 0.0873 0.1271 0.1494 0.1287

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.3451 14.1817 12.7389 18.1159 25.711 20.4453 18.5843 14.1349 17.6149 30.9404
Translational Efficiency 1.2075 1.1925 0.8767 0.8241 1.0008 1.6144 0.7799 0.7989 0.8856 0.9322

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1883 1536 354 202 2050 1448 273 207 3933 2984 627 409

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 785.04 829.99 1199.62 1141.25 841.93 904.78 1131.57 1026.70 814.69 866.28 1169.99 1083.27
Standard Deviation 93.72 121.07 144.48 158.92 103.68 619.47 160.36 183.72 103.03 441.76 155.30 181.21
Intensity Change Log 2 0.080328 0.611739 0.539777 0.103867 0.426554 0.286242 0.092557 0.518879 0.414144

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000134 0.000138 0.000315 0.000763 0.000052 0.000207 0.000213 0.000340 0.000091 0.000171 0.000271 0.000549
Bud Neck 0.003463 0.002721 0.017428 0.012189 0.001982 0.003767 0.016313 0.008511 0.002691 0.003228 0.016943 0.010327
Bud Site 0.009471 0.011866 0.014344 0.064234 0.005642 0.011083 0.021746 0.034013 0.007475 0.011486 0.017567 0.048939
Cell Periphery 0.000136 0.000098 0.000094 0.000109 0.000092 0.000190 0.000089 0.000116 0.000113 0.000143 0.000091 0.000112
Cytoplasm 0.006844 0.012724 0.002121 0.020211 0.006829 0.016433 0.011862 0.012939 0.006836 0.014524 0.006362 0.016531
Cytoplasmic Foci 0.075188 0.112361 0.028908 0.053510 0.056251 0.089055 0.032852 0.039110 0.065317 0.101052 0.030625 0.046222
Eisosomes 0.000032 0.000019 0.000037 0.000013 0.000015 0.000024 0.000027 0.000020 0.000023 0.000021 0.000033 0.000017
Endoplasmic Reticulum 0.001154 0.001449 0.002575 0.000581 0.001174 0.000962 0.001121 0.001678 0.001164 0.001213 0.001942 0.001136
Endosome 0.502114* 0.413967* 0.213275* 0.192930 0.351234* 0.478408* 0.179144 0.230027* 0.423471* 0.445237* 0.198414 0.211705*
Golgi 0.090515 0.071466 0.023757 0.039263 0.052798 0.068565 0.020724 0.025922 0.070856 0.070058 0.022436 0.032511
Lipid Particles 0.004214 0.002989 0.003097 0.000998 0.002932 0.006434 0.001557 0.001950 0.003546 0.004661 0.002427 0.001480
Mitochondria 0.057987 0.020923 0.002370 0.003040 0.028660 0.031213 0.003658 0.004839 0.042701 0.025916 0.002931 0.003950
Mitotic Spindle 0.001420 0.008484 0.030109 0.071850 0.002145 0.008089 0.038537 0.013604 0.001798 0.008292 0.033778 0.042371
None 0.000147 0.000189 0.000085 0.000117 0.000116 0.000922 0.000226 0.000107 0.000131 0.000545 0.000147 0.000112
Nuclear Periphery 0.000150 0.000474 0.011448 0.001741 0.000498 0.000381 0.004729 0.002565 0.000331 0.000429 0.008523 0.002158
Nuclear Periphery Foci 0.000827 0.003207 0.027880 0.004188 0.001335 0.001697 0.007012 0.003582 0.001092 0.002474 0.018794 0.003882
Nucleolus 0.000264 0.000497 0.019031 0.001238 0.000488 0.000827 0.015907 0.005256 0.000380 0.000657 0.017671 0.003272
Nucleus 0.000228 0.000576 0.014790 0.001613 0.000437 0.004340 0.013592 0.004730 0.000337 0.002403 0.014268 0.003190
Peroxisomes 0.003274 0.003858 0.009232 0.007747 0.001510 0.004539 0.006178 0.005378 0.002354 0.004189 0.007902 0.006548
Vacuole 0.132291 0.192674 0.429242* 0.409122* 0.275005* 0.161878 0.469611* 0.482753* 0.206678* 0.177730 0.446819* 0.446387*
Vacuole Periphery 0.110147* 0.139319* 0.149863* 0.114545* 0.210808* 0.110988* 0.154904* 0.122560* 0.162614* 0.125571* 0.152058* 0.118602*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.13 -2.65 -1.42 -1.42 -1.02 -1.93 -3.31 -1.71 -0.73 -0.55 -1.86 -4.15 -1.90 -1.59 -1.12
Bud Neck 1.12 -6.26 -4.49 -4.85 1.80 -2.70 -8.17 -6.31 -4.07 4.37 -1.20 -9.73 -6.83 -6.22 3.92
Bud Site -1.73 -2.50 -5.71 -5.47 -5.06 -4.10 -6.70 -4.58 -3.64 -1.69 -4.23 -6.51 -7.22 -6.51 -5.20
Cell Periphery 2.17 2.30 0.90 -1.06 -1.16 -3.40 -0.19 -1.26 2.20 -1.21 -1.95 1.58 -0.37 1.46 -1.66
Cytoplasm -4.03 5.40 -4.62 -2.90 -5.89 -4.52 -2.15 -2.49 0.90 0.25 -5.99 -0.66 -5.14 -1.56 -3.85
Cytoplasmic Foci -9.63 13.66 3.31 8.65 -3.98 -9.19 5.58 3.50 8.98 -0.92 -13.54 13.28 4.69 12.48 -3.60
Eisosomes 3.52 -1.42 4.88 2.70 6.25 -2.24 -4.21 -1.74 0.91 2.40 0.72 -3.75 2.74 1.83 6.17
Endoplasmic Reticulum -0.84 -4.08 2.78 2.37 5.44 1.02 -0.72 -1.40 -1.87 -0.91 -0.25 -4.06 0.05 0.23 3.47
Endosome 9.54 23.05 21.80 15.46 1.29 -13.05 14.82 8.05 15.52 -3.04 -3.08 25.70 20.26 21.67 -1.11
Golgi 4.55 15.00 8.44 5.47 -2.13 -4.26 8.86 5.19 7.75 -0.97 0.34 16.61 9.63 9.24 -2.17
Lipid Particles 1.62 1.13 3.97 3.73 2.97 -3.02 1.84 1.40 3.98 -0.38 -1.66 1.75 3.77 5.05 2.03
Mitochondria 11.43 19.80 18.62 9.34 -0.54 -0.89 13.58 11.50 10.70 -0.80 7.96 23.95 21.63 14.12 -1.08
Mitotic Spindle -5.40 -5.87 -5.60 -5.08 -3.13 -5.29 -5.57 -3.27 -1.58 3.57 -7.48 -8.06 -6.10 -5.19 -1.07
None -1.05 1.63 0.38 1.35 -0.94 -3.63 -3.08 0.37 3.68 3.32 -3.77 -1.45 0.50 3.87 1.86
Nuclear Periphery -4.26 -6.27 -3.52 -2.81 5.16 1.40 -2.91 -1.57 -1.66 1.30 -1.71 -6.75 -2.65 -2.51 4.59
Nuclear Periphery Foci -2.52 -6.05 -2.83 -1.07 4.95 -0.85 -3.50 -1.33 -1.06 1.79 -2.59 -6.74 -2.84 -1.47 5.23
Nucleolus -1.81 -6.49 -2.63 -2.11 6.03 -1.10 -4.82 -2.77 -2.49 3.23 -1.58 -8.04 -3.35 -2.97 6.38
Nucleus -2.54 -4.99 -2.80 -2.33 4.36 -4.06 -4.47 -3.82 0.18 3.20 -4.33 -6.66 -4.72 -0.99 5.34
Peroxisomes -0.92 -4.87 -2.64 -2.31 0.53 -5.14 -4.23 -3.28 -0.59 0.66 -4.27 -6.64 -4.01 -2.25 0.97
Vacuole -10.02 -22.48 -14.47 -11.07 1.03 16.95 -12.35 -11.37 -17.52 -1.07 6.22 -23.46 -17.81 -19.94 -0.23
Vacuole Periphery -5.29 -3.74 0.24 2.90 2.80 17.24* 6.08 8.20 -0.90 2.00 9.07 2.40 6.33 1.46 3.35