Standard name
Human Ortholog
Description Cytoplasmic 60S subunit biogenesis factor; associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.05 0.06 0.05 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.96 0.96 0.87 0.8 0.73 0.78 0.75 0.69 0.65 0.63 0.99 0.98 0.98 0.94 0.95 0.97 0.95 0.96 0.93 0.96 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.1 0.25 0.32 0.22 0.26 0.38 0.4 0.43 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0.07 0.05 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 2 4 5 4 0 6 3 7 0 1 0 0 0 0 0 0 1 0 1 1
Bud 2 1 3 2 3 9 36 10 15 24 11 20 0 0 0 0 0 0 0 0 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 5 1 28 8 19 23 24 39 32 35 35 40 12 24 7 12 16 6 0 0 1 0 0 0
Cytoplasm 221 340 606 374 414 517 538 565 389 449 365 383 447 665 555 289 361 345 223 326 609 437 526 493
Endoplasmic Reticulum 1 2 10 3 9 12 7 10 15 8 5 11 2 15 12 8 12 7 0 1 17 0 2 6
Endosome 1 0 1 0 0 1 5 4 3 2 0 3 2 1 0 1 0 1 0 2 2 3 1 3
Golgi 0 0 1 0 0 0 0 0 1 0 0 0 1 0 1 2 1 0 0 1 0 1 0 0
Mitochondria 2 2 5 13 48 162 235 158 138 248 223 261 0 0 0 2 3 1 1 1 3 1 1 2
Nucleus 1 1 1 1 2 2 3 1 0 3 0 3 0 2 1 7 3 5 1 0 1 1 2 1
Nuclear Periphery 0 0 1 0 0 0 1 0 2 1 0 1 0 0 0 0 0 0 0 0 2 0 0 4
Nucleolus 1 1 0 0 0 0 2 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Vac/Vac Membrane 2 0 18 7 19 24 53 51 25 42 32 34 0 4 0 11 10 12 3 2 7 2 4 2
Unique Cell Count 230 344 633 390 477 643 741 723 522 654 561 606 450 677 565 308 379 354 235 339 657 457 546 526
Labelled Cell Count 237 349 674 408 516 754 909 842 621 818 675 763 464 713 576 332 406 377 235 339 657 457 546 526


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.9 12.6 12.6 11.4 10.4 8.5 7.7 8.0 6.8 6.7 6.2 6.1 14.7 14.6 13.8 17.5 16.0 15.4 14.7 14.9 16.1
Std Deviation (1e-4) 2.0 2.3 2.1 1.7 1.6 1.8 1.6 1.7 1.3 1.5 1.6 1.5 2.3 2.0 2.0 4.9 3.6 4.0 2.6 2.7 3.3
Intensity Change (Log2) -0.14 -0.27 -0.56 -0.71 -0.65 -0.88 -0.91 -1.02 -1.04 0.23 0.22 0.14 0.48 0.35 0.3 0.22 0.25 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.7 4.9 1.7 3.3 4.0 2.4 3.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.0 -0.4 -0.8 -1.1 0.8 1.3 0.8 1.4 1.7 -1.5 -0.8 -3.3 -0.4 -0.2 -2.3
Cytoplasm 0.1 -5.4 -8.4 -11.4 -9.4 -10.4 -12.6 -13.6 -14.3 3.6 2.7 2.5 -1.3 -0.4 1.4
Endoplasmic Reticulum 0 0.4 0.4 -1.1 -0.3 1.5 -0.5 -1.1 0.3 0 0.8 0.7 1.1 1.7 0.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.0 7.2 12.9 15.0 11.9 13.2 16.8 17.1 18.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.1 1.0 0.9 3.6 3.5 1.7 3.0 2.5 2.4 -3.6 -3.2 -4.0 0.6 -0.2 0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.8698 13.3069 12.7785 12.2312 11.725 12.9753 7.1523 11.9897 11.1002 11.2567 9.8522 11.2095 9.9754 13.0727 11.9885 11.467 10.9115 11.7253
Actin 0.0096 0.0085 0.0022 0.0001 0.0004 0.0011 0.0251 0.0074 0.0011 0.0025 0.0006 0.0027 0.0153 0.0006 0.0103 0.0021 0.0054 0.0022
Bud 0.0003 0.0003 0.0005 0.0003 0.0005 0.0002 0.0012 0.0012 0.0002 0.0063 0.0002 0.0024 0.0014 0.0036 0.0044 0.004 0.0097 0.0017
Bud Neck 0.0009 0.0002 0.0005 0.0003 0.0005 0.0008 0.0047 0.0001 0.0004 0.0007 0.0003 0.0009 0.0006 0.0005 0.0016 0.0005 0.0012 0.0017
Bud Periphery 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0012 0.0002 0.0003 0.0027 0.0001 0.001 0.001 0.0005 0.0017 0.0011 0.0057 0.0007
Bud Site 0.0008 0.0009 0.0131 0.0002 0.0002 0.0001 0.023 0.0007 0.0002 0.0039 0.0002 0.0004 0.0031 0.0015 0.0052 0.0014 0.0089 0.0021
Cell Periphery 0.0005 0.0002 0.0002 0 0 0.0001 0.0006 0.0001 0 0.0005 0 0.0001 0.0002 0.0002 0.0004 0.0002 0.0006 0.0001
Cytoplasm 0.7538 0.9532 0.908 0.9279 0.9549 0.9131 0.6293 0.9733 0.9604 0.8664 0.8381 0.9041 0.8405 0.9602 0.9168 0.9139 0.9057 0.9259
Cytoplasmic Foci 0.0309 0.0062 0.0133 0.027 0.0096 0.0089 0.0479 0.0042 0.0064 0.0185 0.0127 0.0129 0.0257 0.0064 0.0115 0.0087 0.0156 0.0105
Eisosomes 0 0.0001 0 0 0 0 0.0004 0 0 0 0 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0753 0.0038 0.023 0.0054 0.0031 0.0422 0.0335 0.0025 0.0026 0.0031 0.0013 0.0213 0.0204 0.0058 0.0073 0.0048 0.0044 0.0164
Endosome 0.0252 0.0084 0.0182 0.026 0.0202 0.0225 0.0532 0.0027 0.0078 0.0416 0.0662 0.0266 0.0292 0.0114 0.0134 0.0298 0.0193 0.0222
Golgi 0.0113 0.0024 0.0029 0.0011 0.0023 0.0028 0.0096 0.0011 0.0006 0.0008 0.0203 0.0035 0.0081 0.0015 0.0036 0.0065 0.0071 0.0039
Lipid Particles 0.0021 0.0027 0.0039 0.0003 0 0.0001 0.0249 0.0001 0.0001 0.0006 0.0171 0.0001 0.0081 0.0002 0.0034 0.0003 0.0003 0.0002
Mitochondria 0.0027 0.0021 0.0004 0.0003 0.001 0.0018 0.008 0.0004 0.0145 0.0007 0.0178 0.0034 0.0102 0.0002 0.0042 0.0045 0.0039 0.006
None 0.0081 0.0017 0.0009 0.0019 0.0007 0.0009 0.0135 0.0019 0.0007 0.0025 0.0004 0.0053 0.0053 0.0008 0.0016 0.0015 0.0014 0.0006
Nuclear Periphery 0.0222 0.0008 0.0022 0.0009 0.0007 0.0012 0.0385 0.0005 0.0007 0.0014 0.0011 0.0022 0.0065 0.001 0.0019 0.0021 0.001 0.0013
Nucleolus 0.0001 0.0001 0 0 0 0 0.0077 0 0 0.0002 0.0001 0.0003 0.0014 0.0002 0.0003 0.0002 0.0003 0
Nucleus 0.0442 0.0012 0.0013 0.0011 0.0013 0.0011 0.0289 0.0007 0.0007 0.0019 0.0011 0.0073 0.0029 0.002 0.0021 0.0021 0.0025 0.0009
Peroxisomes 0.0011 0.0026 0.0032 0.0001 0.0001 0 0.0257 0.001 0.0002 0.0003 0.0046 0.0002 0.0047 0.0001 0.0041 0.0001 0.0006 0.0002
Punctate Nuclear 0.0073 0.0005 0.0017 0.0007 0.0001 0.0001 0.0142 0.0001 0.0002 0.0004 0.0001 0.0012 0.0056 0.0005 0.0013 0.0003 0.0007 0.0001
Vacuole 0.0026 0.0036 0.0036 0.0051 0.003 0.0021 0.0059 0.0014 0.0021 0.0355 0.0125 0.003 0.0068 0.0024 0.0039 0.0139 0.0045 0.002
Vacuole Periphery 0.0007 0.0004 0.0007 0.001 0.0011 0.0008 0.0028 0.0002 0.0005 0.0096 0.0053 0.0011 0.0029 0.0003 0.0009 0.0021 0.0011 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.842 37.9668 43.9498 48.3064 34.1409 52.6353 50.0965 58.2962 49.2292 45.4758
Translational Efficiency 1.9992 2.2992 1.6319 1.6347 2.014 2.0826 1.7706 1.3511 1.6194 1.4554

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1175 1321 1729 1538 2057 2072 756 1300 3232 3393 2485 2838

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1095.35 1418.12 1380.35 1408.41 1157.47 1321.16 1358.50 1432.73 1134.89 1358.91 1373.70 1419.55
Standard Deviation 176.77 235.86 187.57 193.57 168.84 197.17 192.91 239.11 174.35 218.25 189.48 215.97
Intensity Change Log 2 0.372588 0.333642 0.362675 0.190830 0.231040 0.307792 0.282065 0.281838 0.334738

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000104 0.000865 0.000227 0.000345 0.000153 0.000252 0.000275 0.000873 0.000135 0.000491 0.000241 0.000587
Bud Neck 0.000669 0.000334 0.000695 0.001179 0.000297 0.000266 0.001824 0.001244 0.000432 0.000292 0.001039 0.001209
Bud Site 0.010990 0.011323 0.007624 0.015570 0.011170 0.010389 0.013149 0.020296 0.011104 0.010753 0.009305 0.017734
Cell Periphery 0.000112 0.000185 0.000117 0.000210 0.000132 0.000107 0.000118 0.000574 0.000125 0.000138 0.000118 0.000377
Cytoplasm 0.866247* 0.864275* 0.910729* 0.804922* 0.862727* 0.890104* 0.645988* 0.893869* 0.864007* 0.880048* 0.830188* 0.845666*
Cytoplasmic Foci 0.076076 0.075612 0.030692 0.032367 0.086489 0.060881 0.066669 0.026878 0.082703 0.066616 0.041637 0.029852
Eisosomes 0.000006 0.000004 0.000003 0.000004 0.000004 0.000003 0.000014 0.000005 0.000005 0.000003 0.000006 0.000004
Endoplasmic Reticulum 0.001329 0.001946 0.000394 0.000519 0.002751 0.000911 0.001310 0.000162 0.002234 0.001314 0.000672 0.000355
Endosome 0.002719 0.004749 0.001995 0.004849 0.003158 0.003454 0.009700 0.001967 0.002998 0.003958 0.004339 0.003529
Golgi 0.013340 0.008935 0.001122 0.003442 0.010061 0.010061 0.000615 0.003393 0.011253 0.009623 0.000968 0.003420
Lipid Particles 0.000681 0.001555 0.001484 0.002316 0.000773 0.001042 0.002165 0.003155 0.000739 0.001242 0.001691 0.002701
Mitochondria 0.000673 0.000903 0.000034 0.000204 0.000827 0.000534 0.000036 0.000146 0.000771 0.000678 0.000034 0.000177
Mitotic Spindle 0.000932 0.003457 0.001474 0.005715 0.000697 0.002975 0.001172 0.002990 0.000783 0.003163 0.001382 0.004466
None 0.000854 0.001238 0.000750 0.000181 0.000775 0.000625 0.000297 0.000390 0.000804 0.000864 0.000612 0.000277
Nuclear Periphery 0.000010 0.000035 0.000042 0.000059 0.000013 0.000012 0.000169 0.000021 0.000011 0.000021 0.000080 0.000042
Nuclear Periphery Foci 0.000231 0.001224 0.002182 0.001452 0.000326 0.000492 0.004714 0.001688 0.000291 0.000777 0.002952 0.001560
Nucleolus 0.000068 0.000087 0.000062 0.000112 0.000033 0.000030 0.000468 0.000081 0.000046 0.000052 0.000186 0.000097
Nucleus 0.004232 0.003361 0.011925 0.005528 0.002448 0.002729 0.015017 0.004876 0.003096 0.002975 0.012866 0.005229
Peroxisomes 0.000387 0.000346 0.000179 0.000524 0.000201 0.000226 0.000513 0.000866 0.000268 0.000273 0.000281 0.000680
Vacuole 0.015177 0.014940 0.027900 0.117179 0.010904 0.011694 0.234687* 0.035529 0.012457 0.012958 0.090810 0.079778
Vacuole Periphery 0.005163 0.004625 0.000371 0.003325 0.006063 0.003212 0.001101 0.000997 0.005736 0.003762 0.000593 0.002259

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.60 -5.23 -7.22 1.08 -2.41 -1.98 -4.48 -2.98 -2.55 -2.45 -1.89 -5.59 -3.99 -0.53 -2.88
Bud Neck 4.40 -1.98 -6.44 -11.96 -5.31 0.76 -14.33 -5.84 -6.02 2.31 3.74 -12.45 -9.23 -10.88 -1.90
Bud Site -0.36 3.23 -3.81 -3.23 -6.18 0.62 -2.13 -5.18 -5.65 -3.51 0.39 1.43 -6.13 -6.45 -7.47
Cell Periphery -1.54 -0.96 -4.47 -0.08 -3.92 1.86 1.34 -2.51 -2.66 -2.59 -0.69 0.56 -3.03 -2.74 -3.09
Cytoplasm 0.38 -5.14 9.65 8.95 15.48 -4.64 19.76 -3.26 1.15 -21.23 -3.35 8.04 6.25 9.10 -2.02
Cytoplasmic Foci 0.08 11.84 11.16 11.23 -0.30 6.77 4.93 16.80 10.59 11.55 5.41 15.36 20.07 15.10 6.63
Eisosomes 1.86 2.66 1.61 -0.81 -3.71 1.02 -10.25 -2.25 -2.73 8.37 2.07 -3.62 -0.10 -2.73 4.48
Endoplasmic Reticulum -1.19 2.15 1.97 5.11 -0.99 4.05 3.53 6.00 5.39 6.95 2.67 5.01 5.99 6.91 4.68
Endosome -4.34 1.63 -4.22 0.34 -5.68 -0.80 -9.42 3.57 3.71 11.01 -3.31 -3.95 -1.32 1.77 2.36
Golgi 2.82 8.49 6.49 6.43 -2.52 0.02 18.14 7.28 5.68 -3.02 1.88 16.61 10.11 8.15 -3.76
Lipid Particles -1.51 -7.00 -8.99 -1.61 -3.82 -0.61 -3.81 -5.70 -3.70 -1.93 -1.42 -7.36 -9.20 -4.00 -3.96
Mitochondria -0.37 6.09 4.07 1.17 -2.10 0.75 2.14 1.86 2.79 -1.60 0.26 3.10 2.56 2.02 -2.66
Mitotic Spindle -2.84 -1.74 -3.64 -1.49 -3.09 -3.10 -0.93 -2.48 -0.41 -1.58 -4.23 -2.58 -4.50 -1.66 -3.42
None -0.85 0.75 3.86 2.44 5.39 0.81 3.01 2.54 2.01 -0.98 -0.31 1.71 4.55 3.21 4.35
Nuclear Periphery -3.28 -11.17 -5.40 -2.27 -0.79 0.05 -10.70 -3.41 -3.93 9.56 -2.78 -14.24 -6.04 -3.98 5.45
Nuclear Periphery Foci -6.83 -13.75 -9.20 -1.58 4.71 -2.30 -9.36 -6.15 -5.26 5.75 -6.67 -15.49 -10.10 -5.88 6.93
Nucleolus -0.73 0.17 -6.38 -0.99 -6.73 0.87 -1.28 -6.17 -6.26 1.14 -0.67 -1.38 -10.08 -3.72 0.83
Nucleus 2.04 -17.51 -6.01 -7.32 11.00 -2.61 -14.46 -10.47 -9.29 10.54 0.41 -25.24 -12.34 -12.07 15.96
Peroxisomes 0.28 2.87 -2.16 -1.97 -5.98 -0.91 -5.22 -3.53 -3.40 -1.82 -0.22 -0.36 -4.60 -4.35 -4.35
Vacuole 0.10 -9.83 -23.33 -23.09 -18.42 -0.75 -24.59 -13.85 -13.17 20.70 -0.61 -23.30 -26.08 -25.64 1.51
Vacuole Periphery 0.61 7.44 2.53 2.02 -3.67 4.10 8.64 8.10 4.89 0.15 3.60 11.86 6.55 3.59 -3.64